Strain Name: |
B6;129S6-Chattm1(cre)Lowl/J |
|---|---|
Stock Number: |
006410 |
Availability: | Repository- Live |
Price and Supply Information | |
General Terms and Conditions |
| Genes & Alleles | Chat; Chattm1(cre)Lowl; cre; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Donating Investigator Bradford Lowell, Beth Israel Deaconess Medical Center Generation Nv?+F2 (03-DEC-07) Strain Description
Homozygous mice maintained at The Jackson Laboratory are viable and fertile. An "IRES-Cre" sequence is inserted downstream of the stop codon such that cre expression is controlled by the endogenous Chat gene promoter. Chat gene expression, however, is unaffected. Cre recombinase activity is reported in all cholinergic neurons. These mice may be useful for "Cre-lox" technology applications in neurobiology, including studies of motor function, learning and memory, Alzheimer's disease, and Down syndrome, and in obesity and diabetes research.Strain Development
A targeting vector was designed to insert an internal ribosome entry site-linked Cre recombinase gene (IRES-Cre) downstream of the stop codon of the endogenous gene. The construct was electroporated into 129S6/SvEvTac-derived W4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. Chimeric mice were bred to C57BL/6 mice. The resulting mutant offspring were bred to wildtype siblings, and maintained as such prior to arrival at The Jackson Laboratory.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Chattm1(cre)Lowl | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Bradford B Lowell | ||
| Common Name(s) | ChAT-IRES-Cre; | ||
| Mutation Made By | Bradford Lowell, Beth Israel Deaconess Medical Center | ||
| Strain of Origin | 129S6/SvEvTac | ||
| ES Cell Line Name | W4 | ||
| ES Cell Line Strain | 129S6/SvEvTac | ||
| Site of Expression | Cre recombinase activity is reported in all cholinergic neurons. | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Gene Symbol and Name | Chat, choline acetyltransferase | ||
| Chromosome | 14 | ||
| Gene Common Name(s) | B230380D24Rik; CMS1A; CMS1A2; RIKEN cDNA B230380D24 gene; | ||
| Molecular Note | An internal ribosome entry site-linked Cre recombinase gene (IRES-cre) was inserted downstream of the termination codon of the endogenous choline acetyltransferase gene. Cre recombinase activity is reported in all cholinergic neurons. [MGI Ref ID J:114556] | ||
Control Information
| Control | ||
|---|---|---|
| 101043 B6129SF1/J | (approximate) | |
| 101045 B6129SF2/J | (approximate) | |
| Considerations for Choosing Controls | ||
| Control Pricing Information for JAX® GEMM® Strains | ||
Genotyping Protocols
Chattm1(cre)Lowl
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, homozygous mice may be bred. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying other alleles of Chat
007902 B6.Cg-Tg(RP23-268L19-EGFP)2Mik/J View Strains carrying other alleles of Chat (1 strain)
Additional Web Information
Cre-lox or FLP-FRT Systems
Visit the Alzheimer's Disease Mouse Model Resource site for helpful information on Alzheimer's Disease and research resources.
Animal Health Reports
Room Number AX11
Research Applications
This mouse can be used to support research in many areas including:
cre relatedNeurobiology Research
Alzheimer's Disease
Behavioral and Learning Defects (Down syndrome)
Cre-lox System (Cre Recombinase expression in neural tissue)
Research Tools
Cre-lox System (Cre Recombinase Expression)
Diabetes and Obesity Research
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
Genetics Research (Tissue/Cell Markers: Cre-lox System)
Neurobiology Research
Research Tools
Cre-lox System
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
References
Selected Reference(s)
Lowell BB; Olson D; Yu J. 2006. Development and phenotype of ChAT-IRES-Cre mice MGI Direct Data Submission :. [MGI Ref ID J:114556]
Price and Supply Information
| Strain Name: | B6;129S6-Chattm1(cre)Lowl/J |
| Stock Number: | 006410 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. The shipping destinations are:
- USA, Canada and Mexico
- International
*Pricing for Shipping Destination selected:
International
| Price(s) in US dollars ($) | Genotype(s) Provided | ||||
|---|---|---|---|---|---|
| Individual Mouse Price | $202.50 | Homozygous for Chattm1(cre)Lowl | |||
| Pair | $404.90 | Homozygous for Chattm1(cre)Lowl x Homozygous for Chattm1(cre)Lowl | |||
Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Histology and Tissue Collection Services are available for all JAX® Mice strains. For more information, please contact Customer Service at orderquest@jax.org or 1-207-288-5845. Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. View Control Pricing Information for JAX® Strains. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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Contact Information
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