Strain Name: |
B6.129-Il12btm1Lky/J |
|---|---|
Stock Number: |
006412 |
Availability: | Repository- Live |
General Terms and Conditions |
| Genes & Alleles | Il12b; Il12btm1Lky; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Mating System Heterozygote x Heterozygote (Female x Male) Species laboratory mouse Donating Investigator Richard Locksley, Univ of California San Francisco-HHMI Generation N10+N1F2 (25-APR-08) Strain Description
Mice homozygous for this bicistronic "yet40" allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The IRES-EYFP is inserted downstream of the endogenous stop codon, allowing for normal expression of the endogenous gene and simultaneous EYFP reporter expression. ELISA assays confirm that p40 protein is expressed at similar levels in homozygous mutant and wildtype mice. The EYFP reporter marks dendritic cell (DC) and macrophage lineage cells that produce p40 following stimulation with toll-like receptor (TLR) ligands both in vivo and in vitro. These mice may be useful for labeling activated IL12/23 p40 expressing cells in studies of immunology and immunity, TLR signal cascades, cancer immunity, and vaccine development.Strain Development
A targeting vector was designed to insert an internal ribosomal entry site-enhanced yellow fluorescent protein (EYFP; Clontech) sequence followed by a bovine growth hormone poly(A) signal and a loxP-flanked neomycin resistance cassette downstream of the stop codon in exon 8 of the endogenous gene. The construct was transfected into 129Sv-derived PrmCre embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. Because the ES cells express Cre recombinase under the direction of the mouse protamine 1 promoter (expresses in testis/male germline), the neomycin resistance cassette was deleted in the male germline. Chimeric males were bred to wild-type C57BL/6 females, and heterozygotes were backcrossed to C57BL/6 for at least 10 generations prior to arrival at The Jackson Laboratory. During the backcrossing, the Prm-cre transgene was eliminated from the strain.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Il12btm1Lky | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Richard M Locksley | ||
| Common Name(s) | IL-12/p40 y; Il12btm1(EYFP)Lky; p40 (y); p40-IRES-eYFP; yet40; | ||
| Mutation Made By | Richard Locksley, Univ of California San Francisco-HHMI | ||
| Strain of Origin | 129/Sv | ||
| Gene Symbol and Name | Il12b, interleukin 12b | ||
| Chromosome | 11 | ||
| Gene Common Name(s) | CLMF; CLMF2; IL-12 p40; IL-12B; IL-23 subunit p40; Il-12b; Il-12p40; NKSF; NKSF2; | ||
| Molecular Note | A targeting vector was designed to insert an internal ribosomal entry site-enhanced yellow fluorescent protein (EYFP; Clontech) sequence (followed by a bovine growth hormone poly(A) signal and a loxP-flanked neomycin resistance cassette) downstream of the stop codon in exon 8 of the endogenous gene. [J:112745] | ||
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, homozygous mice are bred. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (135 strains)
002693 B6.129S1-Il12btm1Jm/J 002694 C.129S1-Il12btm1Jm/J
Additional Web Information
Congenic Nomenclature
Fluorescent Proteins/lacZ Systems
Animal Health Reports
Room Number AX11
Research Applications
This mouse can be used to support research in many areas including:
Cancer Research
Growth Factors/Receptors/Cytokines
Immunology and Inflammation Research
Growth Factors/Receptors/Cytokines
Immunodeficiency
Inflammation
Research Tools
Cancer Research
Fluorescent Proteins
Genetics Research (Tissue/Cell Markers: multiple)
Immunology and Inflammation Research
References
Selected Reference(s)
Additional ReferencesReinhardt RL; Hong S; Kang SJ; Wang ZE; Locksley RM. 2006. Visualization of IL-12/23p40 in vivo reveals immunostimulatory dendritic cell migrants that promote Th1 differentiation. J Immunol 177(3):1618-27. [PubMed: 16849470] [J:112745]
Price and Supply Information
| Strain Name: | B6.129-Il12btm1Lky/J |
| Stock Number: | 006412 |
Price Details
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Supply Details
| Standard Supply | Repository-Live. No age specifications accepted. Colony sized to produce minimal quantities (typically up to 6 mice) upon order receipt; one order per strain. Expected delivery: 1-3 months. Larger quantities or custom orders arranged upon request. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
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- Use of these MICE by for-profit entities requires a license from INSTITUTION.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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