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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Homozygote x Homozygote (Female x Male) 11-JUN-08 Species laboratory mouse Generation N10+N1F4 (18-DEC-08) Donating Investigator Richard Locksley, Univ of California San Francisco-HHMI Description
Mice homozygous for this bicistronic "yet40" allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The IRES-EYFP is inserted downstream of the endogenous stop codon, allowing for normal expression of the endogenous gene and simultaneous EYFP reporter expression. ELISA assays confirm that p40 protein is expressed at similar levels in homozygous mutant and wildtype mice. The EYFP reporter marks dendritic cell (DC) and macrophage lineage cells that produce p40 following stimulation with toll-like receptor (TLR) ligands both in vivo and in vitro. These mice may be useful for labeling activated IL12/23 p40 expressing cells in studies of immunology and immunity, TLR signal cascades, cancer immunity, and vaccine development.Development
A targeting vector was designed to insert an internal ribosomal entry site-enhanced yellow fluorescent protein (EYFP; Clontech) sequence followed by a bovine growth hormone poly(A) signal and a loxP-flanked neomycin resistance cassette downstream of the stop codon in exon 8 of the endogenous gene. The construct was transfected into 129Sv-derived PrmCre embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. Because the ES cells express Cre recombinase under the direction of the mouse protamine 1 promoter (expresses in testis/male germline), the neomycin resistance cassette was deleted in the male germline. Chimeric males were bred to wild-type C57BL/6 females, and heterozygotes were backcrossed to C57BL/6 for at least 10 generations prior to arrival at The Jackson Laboratory. During the backcrossing, the Prm-cre transgene was eliminated from the strain.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (225 strains)
009618 NOD.129(B6)-Il12btm1Lky/JbsJ
002693 B6.129S1-Il12btm1Jm/J 002694 C.129S1-Il12btm1Jm/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Il12btm1Lky/Il12btm1Lky
involves: 129S4/SvJae * BALB/c * C57BL/6
- immune system phenotype
- abnormal dendritic cell physiology (MGI Ref ID J:112745)
- migration following CpG stimulation is improved
- increased T cell proliferation (MGI Ref ID J:112745)
- following CpG stimulation, OVA-specific T cells numbers and IL-2 producing cells are increased in lymph nodes
- increased interleukin-2 secretion (MGI Ref ID J:112745)
- IL-2 production is increased following stimulation
- hematopoietic system phenotype
- increased T cell proliferation (MGI Ref ID J:112745)
- following CpG stimulation, OVA-specific T cells numbers and IL-2 producing cells are increased in lymph nodes
This mouse can be used to support research in many areas including:
Cancer Research
Growth Factors/Receptors/Cytokines
Immunology and Inflammation Research
Growth Factors/Receptors/Cytokines
Immunodeficiency
Inflammation
Research Tools
Cancer Research
Fluorescent Proteins
Genetics Research
Tissue/Cell Markers: multiple
Immunology and Inflammation Research
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Il12btm1Lky | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Richard M Locksley | ||
| Allele Type | Targeted (Reporter) | ||
| Common Name(s) | IL-12/p40 y; Il12btm1(EYFP)Lky; p40 (y); p40-IRES-eYFP; yet40; | ||
| Mutation Made By | Richard Locksley, Univ of California San Francisco-HHMI | ||
| Strain of Origin | 129/Sv | ||
| Site of Expression | EYFP is expressed in dendritic cell (DC) and macrophage lineage cells that produce Il12b (p40) following stimulation with toll-like receptor (TLR) ligands both in vivo and in vitro. | ||
| Gene Symbol and Name | Il12b, interleukin 12b | ||
| Chromosome | 11 | ||
| Gene Common Name(s) | CLMF; CLMF2; IL-12 p40; IL-12B; IL-23 subunit p40; Il-12b; Il-12p40; Il12; NKSF; NKSF2; | ||
| Molecular Note | A targeting vector was designed to insert an internal ribosomal entry site-enhanced yellow fluorescent protein (EYFP; Clontech) sequence (followed by a bovine growth hormone poly(A) signal and a loxP-flanked neomycin resistance cassette) downstream of the stop codon in exon 8 of the endogenous gene. [MGI Ref ID J:112745] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Il12btm1Lky STD PCR, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Reinhardt RL; Hong S; Kang SJ; Wang ZE; Locksley RM. 2006. Visualization of IL-12/23p40 in vivo reveals immunostimulatory dendritic cell migrants that promote Th1 differentiation. J Immunol 177(3):1618-27. [PubMed: 16849470] [MGI Ref ID J:112745]
Additional References
Il12btm1Lky relatedKhader SA; Bell GK; Pearl JE; Fountain JJ; Rangel-Moreno J; Cilley GE; Shen F; Eaton SM; Gaffen SL; Swain SL; Locksley RM; Haynes L; Randall TD; Cooper AM. 2007. IL-23 and IL-17 in the establishment of protective pulmonary CD4+ T cell responses after vaccination and during Mycobacterium tuberculosis challenge. Nat Immunol 8(4):369-77. [PubMed: 17351619] [MGI Ref ID J:120806]
Kim S; Yang JY; Lee K; Oh KH; Gi M; Kim JM; Paik DJ; Hong S; Youn J. 2009. Bacillus subtilis-specific poly-gamma-glutamic acid regulates development pathways of naive CD4(+) T cells through antigen-presenting cell-dependent and -independent mechanisms. Int Immunol 21(8):977-90. [PubMed: 19561046] [MGI Ref ID J:151667]
Robinson RT; Khader SA; Locksley RM; Lien E; Smiley ST; Cooper AM. 2008. Yersinia pestis evades TLR4-dependent induction of IL-12(p40)2 by dendritic cells and subsequent cell migration. J Immunol 181(8):5560-7. [PubMed: 18832714] [MGI Ref ID J:140760]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, homozygous mice are bred. Mating System Homozygote x Homozygote (Female x Male) 11-JUN-08 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $160.40 Female or Male Homozygous for Il12btm1Lky
Pairs /Price (US dollars $) Pair Genotype $320.80 Homozygous for Il12btm1Lky x Homozygous for Il12btm1Lky
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $208.60 Female or Male Homozygous for Il12btm1Lky
Pairs /Price (US dollars $) Pair Genotype $417.10 Homozygous for Il12btm1Lky x Homozygous for Il12btm1Lky
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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