Strain Name:

B6;129S4-Mc4rtm1Lowl/J

Stock Number:

006414

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Homozygous mice exhibit severe early-onset obesity, accompanied by hyperphagia, increased snout-anus length and hyperinsulinemia. The function of this disrupted allele can be restored by the enzymatic activity of Cre-recombinase. These mutant mice may be useful in studies of neurobiology, obesity, diabetes, hunger/appetite, and fat and energy metabolism.

Description

Strain Information

Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Mating SystemHeterozygote x Heterozygote         (Female x Male)   02-MAY-07
Specieslaboratory mouse
GenerationF?+N1F1pN1F14 (24-JAN-14)
Generation Definitions
 
Donating Investigator Bradford B. Lowell,   Beth Israel Deaconess Med Cntr (Harvard)

Description
The mice have a loxp-flanked transcriptional blocking (loxTB) sequence that prevents normal endogenous gene transcription and translation from the endogenous locus. As such, homozygous mice are devoid of functional mRNA in all tested regions of the brain. Homozygous mice exhibit severe early-onset obesity, accompanied by hyperphagia, increased snout-anus length and hyperinsulinemia. The function of this disrupted allele can be restored by the enzymatic activity of Cre-recombinase. These mutant mice may be useful in studies of neurobiology, obesity, diabetes, hunger/appetite, and fat and energy metabolism.

When bred to a strain expressing Cre recombinase in the hypothalamus see Stock No. 006395 for example), this mutant mouse strain exhibits as intermediate phenotype in comparison to homozygous null mice.

Development
A targeting vector was designed to insert a loxp-flanked transcriptional blocker sequence (loxTB) between the transcriptional start site and ATG of the endogenous gene. The blocker sequence contains an SV40 enhancer, neomycin resistance gene, two HSV-TK polyA sequences, and an additional transcriptional pause signal from the pGL3-control vector. The construct was electroporated into 129S4/SvJae-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. Chimeric mice were bred to C57BL/6 mice. Mutant mice heterozygous for the "loxTB Mc4r" allele were bred together and maintained as such prior to arrival at The Jackson Laboratory.

Control Information

  Control
   Wild-type from the colony
   101043 B6129SF1/J (approximate)
   101045 B6129SF2/J (approximate)
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Mc4r
008323   B6.Cg-Tg(Mc4r-MAPT/Sapphire)21Rck/J
008330   B6.Cg-Tg(Mc4r-cre)25Rck/J
View Strains carrying other alleles of Mc4r     (2 strains)

Additional Web Information

Introduction to Cre-lox technology

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
Models with phenotypic similarity to human diseases where etiology is unknown or involving genes where ortholog is unknown.
Fatty Liver Disease, Nonalcoholic, Susceptibility to, 1; NAFLD1
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Obesity   (MC4R)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Mc4rtm1Lowl/Mc4r+

        involves: 129S4/SvJae * C57BL/6
  • growth/size/body phenotype
  • obese   (MGI Ref ID J:199365)

Mc4rtm1Lowl/Mc4rtm1Lowl

        involves: 129S4/SvJae * C57BL/6J
  • growth/size/body phenotype
  • increased body length
    • at 12 weeks, homozygotes show increased snout-anus length   (MGI Ref ID J:115192)
  • increased susceptibility to weight gain
    • mutants exhibit increased weight gain compared to wild-type mice when fed either the standard diet or a high-fat diet   (MGI Ref ID J:177386)
    • increased susceptibility to diet-induced obesity
      • mutants exhibit increased weight gain compared to wild-type mice when fed a high-fat diet   (MGI Ref ID J:177386)
  • increased total body fat amount
    • mutants fed the standard diet exhibit increased adiposity compared to wild-type mice on the same diet   (MGI Ref ID J:177386)
    • mutants fed the high-fat diet also exhibit increased adiposity, but only up to 8 weeks of high-fat feeding with no further increase after 8 weeks   (MGI Ref ID J:177386)
  • obese   (MGI Ref ID J:177386)
    • male and female homozygotes display severe early-onset obesity   (MGI Ref ID J:115192)
  • homeostasis/metabolism phenotype
  • abnormal oxygen consumption
    • mice do not show an increase in oxygen consumption from stimulation by MTII as wild-type mice do   (MGI Ref ID J:115192)
    • decreased oxygen consumption
      • oxygen consumption is reduced in the dark and light cycles compared to wild-type and is similar to mutants also expressing Tg(Sim1-cre)1Lowl   (MGI Ref ID J:115192)
  • decreased adiponectin level
    • mutants fed the high-fat diet exhibit decreased serum adiponectin levels compared to wild-type mice on the same diet   (MGI Ref ID J:177386)
  • increased circulating alanine transaminase level
    • mutants fed the high-fat diet show increased serum alanine aminotransferase concentrations compared to wild-type mice   (MGI Ref ID J:177386)
  • increased circulating free fatty acid level
    • mutants fed the high-fat diet show an increase in serum free fatty acid concentrations relative to wild-type mice   (MGI Ref ID J:177386)
  • increased circulating insulin level
    • hyperinsulinemia is displayed by homozygotes   (MGI Ref ID J:115192)
  • increased circulating interleukin-6 level
    • mutants fed the high-fat diet exhibit increased serum IL-6 levels compared to wild-type mice on the same diet   (MGI Ref ID J:177386)
  • increased circulating leptin level
    • mutants fed the high-fat diet exhibit increased serum leptin levels compared to wild-type mice on the same diet   (MGI Ref ID J:177386)
  • increased liver triglyceride level
    • mutants exhibit increased hepatic triglyceride content compared to wild-type mice on either the standard or high-fat diet   (MGI Ref ID J:177386)
    • livers of mutants fed the high-fat diet exhibit increased triglyceride secretion compared to wild-type livers   (MGI Ref ID J:177386)
  • increased susceptibility to diet-induced obesity
    • mutants exhibit increased weight gain compared to wild-type mice when fed a high-fat diet   (MGI Ref ID J:177386)
  • liver fibrosis
    • livers from mutants fed the high-fat diet exhibit increased pericellular fibrosis compared to wild-type mice at 8 and 20 weeks of high-fat feeding   (MGI Ref ID J:177386)
  • behavior/neurological phenotype
  • abnormal food intake
    • unlike wild-type mice, mutants do not exhibit a reduction in food intake after treatment with the Mc4r inhibitor, MTII   (MGI Ref ID J:115192)
    • polyphagia
      • obesity is accompanied by polyphagia   (MGI Ref ID J:115192)
  • adipose tissue phenotype
  • abnormal white adipose tissue morphology
    • white adipose tissue of mutants fed the high-fat diet for 8 weeks exhibits increased macrophage infiltration and pro-inflammatory cytokine expression compared to wild-type white adipose tissue   (MGI Ref ID J:177386)
  • increased total body fat amount
    • mutants fed the standard diet exhibit increased adiposity compared to wild-type mice on the same diet   (MGI Ref ID J:177386)
    • mutants fed the high-fat diet also exhibit increased adiposity, but only up to 8 weeks of high-fat feeding with no further increase after 8 weeks   (MGI Ref ID J:177386)
  • immune system phenotype
  • increased circulating interleukin-6 level
    • mutants fed the high-fat diet exhibit increased serum IL-6 levels compared to wild-type mice on the same diet   (MGI Ref ID J:177386)
  • liver inflammation
    • livers from mutants fed the high-fat diet exhibit ballooning degeneration and massive infiltration of inflammatory cells   (MGI Ref ID J:177386)
    • livers from mutants fed the high-fat diet exhibit increased inflammatory cell infiltration compared to wild-type livers   (MGI Ref ID J:177386)
  • liver/biliary system phenotype
  • hepatic steatosis
    • mutants fed the high-fat diet for 8 weeks exhibit massive microvesicular steatosis in the centrilobular and portal areas while wild-type mice only have minimal lipid accumulation in the liver   (MGI Ref ID J:177386)
    • mutants fed the high-fat diet develop liver steatosis faster (by 8 weeks of high-fat diet) than wild-type mice (by 20 weeks of high-fat diet)   (MGI Ref ID J:177386)
  • increased liver triglyceride level
    • mutants exhibit increased hepatic triglyceride content compared to wild-type mice on either the standard or high-fat diet   (MGI Ref ID J:177386)
    • livers of mutants fed the high-fat diet exhibit increased triglyceride secretion compared to wild-type livers   (MGI Ref ID J:177386)
  • increased liver weight
    • mutants exhibit increased liver weight compared to wild-type mice on either the standard or high-fat diet   (MGI Ref ID J:177386)
  • liver fibrosis
    • livers from mutants fed the high-fat diet exhibit increased pericellular fibrosis compared to wild-type mice at 8 and 20 weeks of high-fat feeding   (MGI Ref ID J:177386)
  • liver inflammation
    • livers from mutants fed the high-fat diet exhibit ballooning degeneration and massive infiltration of inflammatory cells   (MGI Ref ID J:177386)
    • livers from mutants fed the high-fat diet exhibit increased inflammatory cell infiltration compared to wild-type livers   (MGI Ref ID J:177386)
  • tumorigenesis
  • increased hepatocellular carcinoma incidence
    • mutants fed the high-fat diet, but not the standard diet, for a year develop liver tumors   (MGI Ref ID J:177386)
    • tumors resemble hepatocellular carcinoma   (MGI Ref ID J:177386)

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Mc4rtm1Lowl/Mc4rtm1Lowl

        involves: 129S4/SvJae
  • growth/size/body phenotype
  • obese
    • become obese but not hypertensive   (MGI Ref ID J:193452)

The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.

Mc4rtm1Lowl/Mc4rtm1Lowl Tg(Nes-cre)1Kln/0

        involves: 129S4/SvJae * C57BL/6 * SJL   (conditional)
  • normal phenotype
  • no abnormal phenotype detected
    • mice homozygous for reactivated Mc4r in neurons show normal body weights and rescue of the increased snout-anus length observed in Mc4r homozygotes   (MGI Ref ID J:115192)

Mc4rtm1Lowl/Mc4rtm1Lowl Tg(Sim1-cre)1Lowl/0

        involves: 129S4/SvJae * C57BL/6J * FVB   (conditional)
  • growth/size/body phenotype
  • *normal* growth/size/body phenotype
    • at 12 weeks, transgenic mice and wild-type littermates have the same snout-anus length, compared to increased length observed in nontransgenic Mc4rtm1Lowl mice   (MGI Ref ID J:115192)
    • increased body weight
      • transgenic mice have increased fat mass compared with wild-type littermates, but significantly decreased fat mass compared to Mc4rtm1Lowl littermates   (MGI Ref ID J:115192)
      • obese
        • at 12 weeks of age, male and female mutants have only 37% and 46% respectively, of the obesity observed in mice homozygous for Mc4r disruption   (MGI Ref ID J:115192)
    • increased lean body mass
      • transgenic mice have increased lean mass compared with wild-type littermates, but significantly decreased lean mass compared to Mc4rtm1Lowl littermates   (MGI Ref ID J:115192)
  • homeostasis/metabolism phenotype
  • abnormal oxygen consumption
    • mice do not show an increase in oxygen consumption from stimulation by MTII as wild-type mice do   (MGI Ref ID J:115192)
    • decreased oxygen consumption
      • oxygen consumption is reduced in the dark and light cycles compared to wild-type and is similar to Mc4rtm1Lowl homozygotes   (MGI Ref ID J:115192)
  • behavior/neurological phenotype
  • *normal* behavior/neurological phenotype
    • transgenic mice are obese, yet show food intake comparable to wild-type mice at 11 weeks of age   (MGI Ref ID J:115192)
    • abnormal food intake
      • when treated with MTII an Mc4r inhibitor, wild-type mice show a 50% reduction in food intake, while transgenic mice display no effect on intake   (MGI Ref ID J:115192)

Mc4rtm1Lowl/Mc4rtm1Lowl Tg(Zp3-cre)3Mrt/0

        involves: 129S4/SvJae * C57BL/6J * FVB/N   (conditional)
  • normal phenotype
  • no abnormal phenotype detected
    • mice homozygous for reactivated Mc4r in neurons show normal body weights and rescue of the increased snout-anus length observed in Mc4r homozygotes mice homozygous for reactivated Mc4r in the germline show normal body weights and rescue of the increased snout-anus length observed in Mc4r homozygotes   (MGI Ref ID J:115192)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Diabetes and Obesity Research
Hyperinsulinemia
Obesity With Diabetes
Obesity Without Diabetes

Neurobiology Research
Metabolic Defects

Research Tools
Cre-lox System
      loxP-flanked Sequences
Diabetes and Obesity Research
      loxP
Metabolism Research
Neurobiology Research

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Mc4rtm1Lowl
Allele Name targeted mutation 1, Bradford B Lowell
Allele Type Targeted (Conditional ready (e.g. floxed), No functional change)
Common Name(s) Mc4rtb; loxTB Mc4r;
Mutation Made By Bradford Lowell,   Beth Israel Deaconess Med Cntr (Harvard)
Strain of Origin129S4/SvJae
ES Cell Line NameJ1
ES Cell Line Strain129S4/SvJae
Gene Symbol and Name Mc4r, melanocortin 4 receptor
Chromosome 18
Gene Common Name(s) Fatboy; Glu3; glucose 3;
Molecular Note A loxP flanked PGK-neomycin selection marker inserted in the 5' UTR created a null allele. Cre recombinase deletes the selection marker, restoring normal activity of the allele. Mice fail to respond to MC4R agonist MTII. This is a null allele that canbe "reactivated" by cre recombinase. [MGI Ref ID J:115192]

Genotyping

Genotyping Information

Genotyping Protocols

Mc4rtm1Lowl, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Additional References

Mc4rtm1Lowl related

Arteaga-Solis E; Zee T; Emala CW; Vinson C; Wess J; Karsenty G. 2013. Inhibition of leptin regulation of parasympathetic signaling as a cause of extreme body weight-associated asthma. Cell Metab 17(1):35-48. [PubMed: 23312282]  [MGI Ref ID J:195073]

Asai M; Ramachandrappa S; Joachim M; Shen Y; Zhang R; Nuthalapati N; Ramanathan V; Strochlic DE; Ferket P; Linhart K; Ho C; Novoselova TV; Garg S; Ridderstrale M; Marcus C; Hirschhorn JN; Keogh JM; O'Rahilly S; Chan LF; Clark AJ; Farooqi IS; Majzoub JA. 2013. Loss of function of the melanocortin 2 receptor accessory protein 2 is associated with mammalian obesity. Science 341(6143):275-8. [PubMed: 23869016]  [MGI Ref ID J:199365]

Balthasar N; Dalgaard LT; Lee CE; Yu J; Funahashi H; Williams T; Ferreira M; Tang V; McGovern RA; Kenny CD; Christiansen LM; Edelstein E; Choi B; Boss O; Aschkenasi C; Zhang CY; Mountjoy K; Kishi T; Elmquist JK; Lowell BB. 2005. Divergence of melanocortin pathways in the control of food intake and energy expenditure. Cell 123(3):493-505. [PubMed: 16269339]  [MGI Ref ID J:115192]

Cui H; Lutter M. 2013. The expression of MC4Rs in D1R neurons regulates food intake and locomotor sensitization to cocaine. Genes Brain Behav 12(6):658-65. [PubMed: 23786641]  [MGI Ref ID J:213389]

Gao Y; Ottaway N; Schriever SC; Legutko B; Garcia-Caceres C; de la Fuente E; Mergen C; Bour S; Thaler JP; Seeley RJ; Filosa J; Stern JE; Perez-Tilve D; Schwartz MW; Tschop MH; Yi CX. 2014. Hormones and diet, but not body weight, control hypothalamic microglial activity. Glia 62(1):17-25. [PubMed: 24166765]  [MGI Ref ID J:203061]

Goncalves GH; Li W; Garcia AV; Figueiredo MS; Bjorbaek C. 2014. Hypothalamic agouti-related peptide neurons and the central melanocortin system are crucial mediators of leptin's antidiabetic actions. Cell Rep 7(4):1093-103. [PubMed: 24813890]  [MGI Ref ID J:211797]

Guan X; Shi X; Li X; Chang B; Wang Y; Li D; Chan L. 2012. GLP-2 receptor in POMC neurons suppresses feeding behavior and gastric motility. Am J Physiol Endocrinol Metab 303(7):E853-64. [PubMed: 22829581]  [MGI Ref ID J:189593]

Itoh M; Kato H; Suganami T; Konuma K; Marumoto Y; Terai S; Sakugawa H; Kanai S; Hamaguchi M; Fukaishi T; Aoe S; Akiyoshi K; Komohara Y; Takeya M; Sakaida I; Ogawa Y. 2013. Hepatic crown-like structure: a unique histological feature in non-alcoholic steatohepatitis in mice and humans. PLoS One 8(12):e82163. [PubMed: 24349208]  [MGI Ref ID J:209729]

Itoh M; Suganami T; Nakagawa N; Tanaka M; Yamamoto Y; Kamei Y; Terai S; Sakaida I; Ogawa Y. 2011. Melanocortin 4 receptor-deficient mice as a novel mouse model of nonalcoholic steatohepatitis. Am J Pathol 179(5):2454-63. [PubMed: 21906580]  [MGI Ref ID J:177386]

Karlsson-Lindahl L; Schmidt L; Haage D; Hansson C; Taube M; Egeciouglu E; Tan YX; Admyre T; Jansson JO; Vlodavsky I; Li JP; Lindahl U; Dickson SL. 2012. Heparanase affects food intake and regulates energy balance in mice. PLoS One 7(3):e34313. [PubMed: 22479599]  [MGI Ref ID J:187121]

Kawahara Y; Grimberg A; Teegarden S; Mombereau C; Liu S; Bale TL; Blendy JA; Nishikura K. 2008. Dysregulated editing of serotonin 2C receptor mRNAs results in energy dissipation and loss of fat mass. J Neurosci 28(48):12834-44. [PubMed: 19036977]  [MGI Ref ID J:142503]

Krashes MJ; Shah BP; Koda S; Lowell BB. 2013. Rapid versus delayed stimulation of feeding by the endogenously released AgRP neuron mediators GABA, NPY, and AgRP. Cell Metab 18(4):588-95. [PubMed: 24093681]  [MGI Ref ID J:206003]

Nogueiras R; Wiedmer P; Perez-Tilve D; Veyrat-Durebex C; Keogh JM; Sutton GM; Pfluger PT; Castaneda TR; Neschen S; Hofmann SM; Howles PN; Morgan DA; Benoit SC; Szanto I; Schrott B; Schurmann A; Joost HG; Hammond C; Hui DY; Woods SC; Rahmouni K; Butler AA; Farooqi IS; O'Rahilly S; Rohner-Jeanrenaud F; Tschop MH. 2007. The central melanocortin system directly controls peripheral lipid metabolism. J Clin Invest 117(11):3475-88. [PubMed: 17885689]  [MGI Ref ID J:127528]

Pillot B; Duraffourd C; Begeot M; Joly A; Luquet S; Houberdon I; Naville D; Vigier M; Gautier-Stein A; Magnan C; Mithieux G. 2011. Role of hypothalamic melanocortin system in adaptation of food intake to food protein increase in mice. PLoS One 6(4):e19107. [PubMed: 21544212]  [MGI Ref ID J:172374]

Rossi J; Balthasar N; Olson D; Scott M; Berglund E; Lee CE; Choi MJ; Lauzon D; Lowell BB; Elmquist JK. 2011. Melanocortin-4 receptors expressed by cholinergic neurons regulate energy balance and glucose homeostasis. Cell Metab 13(2):195-204. [PubMed: 21284986]  [MGI Ref ID J:169562]

Sohn JW; Harris LE; Berglund ED; Liu T; Vong L; Lowell BB; Balthasar N; Williams KW; Elmquist JK. 2013. Melanocortin 4 receptors reciprocally regulate sympathetic and parasympathetic preganglionic neurons. Cell 152(3):612-9. [PubMed: 23374353]  [MGI Ref ID J:193452]

Thiebaud N; Johnson MC; Butler JL; Bell GA; Ferguson KL; Fadool AR; Fadool JC; Gale AM; Gale DS; Fadool DA. 2014. Hyperlipidemic diet causes loss of olfactory sensory neurons, reduces olfactory discrimination, and disrupts odor-reversal learning. J Neurosci 34(20):6970-84. [PubMed: 24828650]  [MGI Ref ID J:211263]

Vella KR; Ramadoss P; Lam FS; Harris JC; Ye FD; Same PD; O'Neill NF; Maratos-Flier E; Hollenberg AN. 2011. NPY and MC4R signaling regulate thyroid hormone levels during fasting through both central and peripheral pathways. Cell Metab 14(6):780-90. [PubMed: 22100407]  [MGI Ref ID J:179670]

Voss-Andreae A; Murphy JG; Ellacott KL; Stuart RC; Nillni EA; Cone RD; Fan W. 2007. Role of the central melanocortin circuitry in adaptive thermogenesis of brown adipose tissue. Endocrinology 148(4):1550-60. [PubMed: 17194736]  [MGI Ref ID J:129583]

Xu Y; Jones JE; Lauzon DA; Anderson JG; Balthasar N; Heisler LK; Zinn AR; Lowell BB; Elmquist JK. 2010. A serotonin and melanocortin circuit mediates D-fenfluramine anorexia. J Neurosci 30(44):14630-4. [PubMed: 21048120]  [MGI Ref ID J:166702]

Xu Y; Wu Z; Sun H; Zhu Y; Kim ER; Lowell BB; Arenkiel BR; Xu Y; Tong Q. 2013. Glutamate mediates the function of melanocortin receptor 4 on Sim1 neurons in body weight regulation. Cell Metab 18(6):860-70. [PubMed: 24315371]  [MGI Ref ID J:206137]

do Carmo JM; da Silva AA; Rushing JS; Pace B; Hall JE. 2013. Differential control of metabolic and cardiovascular functions by melanocortin-4 receptors in proopiomelanocortin neurons. Am J Physiol Regul Integr Comp Physiol 305(4):R359-68. [PubMed: 23842677]  [MGI Ref ID J:201516]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX12

Colony Maintenance

Breeding & HusbandryAlthough homozygous mice are viable and fertile, the donating investigator breeds heterozygous mice together to generate homozygous, heterozygous, and wildtype mice.
Mating SystemHeterozygote x Heterozygote         (Female x Male)   02-MAY-07
Diet Information LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $199.90Female or MaleHeterozygous for Mc4rtm1Lowl  
$199.90Female or MaleHomozygous for Mc4rtm1Lowl  
Price per Pair (US dollars $)Pair Genotype
$399.80Heterozygous for Mc4rtm1Lowl x Heterozygous for Mc4rtm1Lowl  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $259.90Female or MaleHeterozygous for Mc4rtm1Lowl  
$259.90Female or MaleHomozygous for Mc4rtm1Lowl  
Price per Pair (US dollars $)Pair Genotype
$519.80Heterozygous for Mc4rtm1Lowl x Heterozygous for Mc4rtm1Lowl  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Control Information

  Control
   Wild-type from the colony
   101043 B6129SF1/J (approximate)
   101045 B6129SF2/J (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


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