Strain Name:

B6;129S1-Map2k3tm1Flv/J

Stock Number:

006416

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Availability:

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Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names B6.129S1-Map2k3tm1Flv/J    (Changed: 24-MAY-10 )
Type Congenic; Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
 
Donating InvestigatorDr. Richard A. Flavell,   Yale University School of Medicine

Description
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. However, the donating investigator reports that reproductive performance is poor for unknown reasons. No gene product (mRNA or protein) is detected by Northern blot analysis of liver and kidney or Western blot analysis of peritoneal macrophages. Mutant mice exhibit an impaired type I cytokine immune response with reduced induced interferon gamma production. Macrophages from mutant mice have a reduced p39 MAPK activation and IL-12 production when challenged with LPS lipopolysaccharide). Dendritic cells also have reduced IL-12 production response. Mutant mouse embryo fibroblasts (MEF) have a reduced cytokine expression response to tumor necrosis factor (TNF). This mutant mouse strain may be useful in studies of inflammatory response.

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.

Development
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt exons 8 and 9. The construct was electroporated into 129S1/Sv-p+ Tyr+ Kitl+ derived W9.5 embryonic stem (ES) cells, and correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were crossed to C57BL/6N mice for at least 10 generations. Upon arriving at The Jackson Laboratory, the mice were crossed with C57BL/6J at least once to establish the colony. SNP (single nucleotide polymorphism) analysis performed by The Jackson Laboratory revealed that this strain is a mixed genetic background (5 out of 27 markers were segregating for non-C57BL/6 alleles).

Control Information

  Control
   Wild-type from the colony
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Map2k3
010582   FVB-Tg(Myh6-Map2k3*)1Jmol/J
View Strains carrying other alleles of Map2k3     (1 strain)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Map2k3tm1Flv/Map2k3tm1Flv

        B6.129S1-Map2k3tm1Flv
  • mortality/aging
  • increased sensitivity to induced morbidity/mortality
    • following infection with live Streptococcus pneumoniae, 80% of mice are dead by day 5 and 100% at day 7 compared to 25% and 66% at the same time points for infected wild-type mice   (MGI Ref ID J:124327)
    • following infection with S. pneumoniae wild-type lysate and S. pneumoniae pneumolysin (PLY), mice begin to die within hours of infection and all mice are dead by 6 hours   (MGI Ref ID J:124327)
  • immune system phenotype
  • increased susceptibility to bacterial infection
    • following infection with live Streptococcus pneumoniae, 80% of mice are dead by day 5 and 100% at day 7 compared to 25% and 66% at the same time points for infected wild-type mice   (MGI Ref ID J:124327)
    • following infection with S. pneumoniae wild-type lysate and S. pneumoniae pneumolysin (PLY), mice begin to die within hours of infection and all mice are dead by 6 hours   (MGI Ref ID J:124327)
    • following infection with S. pneumoniae, mice exhibit more massive alveolar hemorrhage compared to infected wild-type mice   (MGI Ref ID J:124327)

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Map2k3tm1Flv/Map2k3tm1Flv

        involves: 129S1/Sv
  • immune system phenotype
  • abnormal CD4-positive, alpha-beta T cell physiology
    • CD4+T cells are resistant to activation-induced cell death and cytokine-withdrawal-induced apoptosis   (MGI Ref ID J:85266)
  • decreased T cell apoptosis
    • CD4+T cells are resistant to activation-induced cell death and cytokine-withdrawal-induced apoptosis   (MGI Ref ID J:85266)
  • cellular phenotype
  • decreased T cell apoptosis
    • CD4+T cells are resistant to activation-induced cell death and cytokine-withdrawal-induced apoptosis   (MGI Ref ID J:85266)
  • hematopoietic system phenotype
  • abnormal CD4-positive, alpha-beta T cell physiology
    • CD4+T cells are resistant to activation-induced cell death and cytokine-withdrawal-induced apoptosis   (MGI Ref ID J:85266)
  • decreased T cell apoptosis
    • CD4+T cells are resistant to activation-induced cell death and cytokine-withdrawal-induced apoptosis   (MGI Ref ID J:85266)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Immunology, Inflammation and Autoimmunity Research
Growth Factors/Receptors/Cytokines

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Map2k3tm1Flv
Allele Name targeted mutation 1, Richard A Flavell
Allele Type Targeted (Null/Knockout)
Common Name(s) MKK3 KO; Mkk3-;
Strain of Origin129S1/Sv-Oca2<+> Tyr<+> Kitl<+>
ES Cell Line NameW9.5/W95
ES Cell Line Strain129S1/Sv-Oca2<+> Tyr<+> Kitl<+>
Gene Symbol and Name Map2k3, mitogen-activated protein kinase kinase 3
Chromosome 11
Gene Common Name(s) AW212142; MAP kinase kinase 3; MAPKK3; MEK3; MKK3; PRKMK3; Prkmk3; SAPKK-2; SAPKK2; expressed sequence AW212142; protein kinase, mitogen-activated, kinase 3;
General Note Homozygous mutant mice are viable and appear normal, healthy, and fertile with no defects in lymphocyte development (thymocytes, splenocytes, and bone-marrow derived dendritic cells). Compared to control sibs, however, macrophages and primary embryonic fibroblasts from mutant mice show reduced activation of p38 MAPK. Cytokine IL-12 (encoded for by Il12a and Il12b) is strongly reduced in mutant macrophages and dendritic cells. Mutant fibroblasts have a defective response to Tnf. Mutant mice also have defective T-helper cells with respect to the ability to produce Ifng in response to antigen stimulation of keyhole limpet hemocyanin (KLH) both in vitro and in vivo (J:54078, J:54309).
Molecular Note A neomycin resistance cassette replaced a 1.5kb region that includes exons 8 and 9, which encode amino acids 217-221 of the protein. This region includes the sequences containing the dual phosphorylation sites (serine and threonine) that are required foractivation of the protein. Northern blot and Western blot analyses did not detect expression from this allele in homozygous mutant mice. [MGI Ref ID J:54309]

Genotyping

Genotyping Information

Genotyping Protocols

Map2k3tm1Flv, Separated PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Lu HT; Yang DD; Wysk M; Gatti E; Mellman I; Davis RJ; Flavell RA. 1999. Defective IL-12 production in mitogen-activated protein (MAP) kinase kinase 3 (Mkk3)-deficient mice. EMBO J 18(7):1845-57. [PubMed: 10202148]  [MGI Ref ID J:54309]

Wysk M; Yang DD; Lu HT; Flavell RA; Davis RJ. 1999. Requirement of mitogen-activated protein kinase kinase 3 (MKK3) for tumor necrosis factor-induced cytokine expression. Proc Natl Acad Sci U S A 96(7):3763-8. [PubMed: 10097111]  [MGI Ref ID J:54078]

Additional References

Map2k3tm1Flv related

Brancho D; Tanaka N; Jaeschke A; Ventura JJ; Kelkar N; Tanaka Y; Kyuuma M; Takeshita T; Flavell RA; Davis RJ. 2003. Mechanism of p38 MAP kinase activation in vivo. Genes Dev 17(16):1969-78. [PubMed: 12893778]  [MGI Ref ID J:84877]

Bulat N; Waeber G; Widmann C. 2009. LDLs stimulate p38 MAPKs and wound healing through SR-BI independently of Ras and PI3 kinase. J Lipid Res 50(1):81-9. [PubMed: 18757346]  [MGI Ref ID J:146100]

Cecil DL; Appleton CT; Polewski MD; Mort JS; Schmidt AM; Bendele A; Beier F; Terkeltaub R. 2009. The pattern recognition receptor CD36 is a chondrocyte hypertrophy marker associated with suppression of catabolic responses and promotion of repair responses to inflammatory stimuli. J Immunol 182(8):5024-31. [PubMed: 19342682]  [MGI Ref ID J:147493]

Clark JE; Flavell RA; Faircloth ME; Davis RJ; Heads RJ; Marber MS. 2007. Post-infarction remodeling is independent of mitogen-activated protein kinase kinase 3 (MKK3). Cardiovasc Res 74(3):466-70. [PubMed: 17399693]  [MGI Ref ID J:162733]

Dolinay T; Wu W; Kaminski N; Ifedigbo E; Kaynar AM; Szilasi M; Watkins SC; Ryter SW; Hoetzel A; Choi AM. 2008. Mitogen-activated protein kinases regulate susceptibility to ventilator-induced lung injury. PLoS ONE 3(2):e1601. [PubMed: 18270588]  [MGI Ref ID J:132187]

Escolano A; Martinez-Martinez S; Alfranca A; Urso K; Izquierdo HM; Delgado M; Martin F; Sabio G; Sancho D; Gomez-del Arco P; Redondo JM. 2014. Specific calcineurin targeting in macrophages confers resistance to inflammation via MKP-1 and p38. EMBO J 33(10):1117-33. [PubMed: 24596247]  [MGI Ref ID J:210829]

Fukuda K; Tesch GH; Yap FY; Forbes JM; Flavell RA; Davis RJ; Nikolic-Paterson DJ. 2008. MKK3 signalling plays an essential role in leukocyte-mediated pancreatic injury in the multiple low-dose streptozotocin model. Lab Invest 88(4):398-407. [PubMed: 18283273]  [MGI Ref ID J:133320]

Gao Y; Tao J; Li MO; Zhang D; Chi H; Henegariu O; Kaech SM; Davis RJ; Flavell RA; Yin Z. 2005. JNK1 is essential for CD8+ T cell-mediated tumor immune surveillance. J Immunol 175(9):5783-9. [PubMed: 16237070]  [MGI Ref ID J:119340]

Gonzalez-Teran B; Cortes JR; Manieri E; Matesanz N; Verdugo A; Rodriguez ME; Gonzalez-Rodriguez A; Valverde A; Martin P; Davis RJ; Sabio G. 2013. Eukaryotic elongation factor 2 controls TNF-alpha translation in LPS-induced hepatitis. J Clin Invest 123(1):164-78. [PubMed: 23202732]  [MGI Ref ID J:194180]

Hegazi RA; Rao KN; Mayle A; Sepulveda AR; Otterbein LE; Plevy SE. 2005. Carbon monoxide ameliorates chronic murine colitis through a heme oxygenase 1-dependent pathway. J Exp Med 202(12):1703-13. [PubMed: 16365149]  [MGI Ref ID J:118826]

Inoue T; Boyle DL; Corr M; Hammaker D; Davis RJ; Flavell RA; Firestein GS. 2006. Mitogen-activated protein kinase kinase 3 is a pivotal pathway regulating p38 activation in inflammatory arthritis. Proc Natl Acad Sci U S A 103(14):5484-9. [PubMed: 16567640]  [MGI Ref ID J:108294]

Kang Y; Wang F; Lu Z; Ying H; Zhang H; Ding W; Wang C; Shi L. 2013. MAPK kinase 3 potentiates Chlamydia HSP60-induced inflammatory response through distinct activation of NF-kappaB. J Immunol 191(1):386-94. [PubMed: 23729445]  [MGI Ref ID J:205361]

Kim L; Del Rio L; Butcher BA; Mogensen TH; Paludan SR; Flavell RA; Denkers EY. 2005. p38 MAPK autophosphorylation drives macrophage IL-12 production during intracellular infection. J Immunol 174(7):4178-84. [PubMed: 15778378]  [MGI Ref ID J:97936]

Kim SI; Na HJ; Ding Y; Wang Z; Lee SJ; Choi ME. 2012. Autophagy promotes intracellular degradation of type I collagen induced by transforming growth factor (TGF)-beta1. J Biol Chem 287(15):11677-88. [PubMed: 22351764]  [MGI Ref ID J:184288]

Kontoyiannis D; Kotlyarov A; Carballo E; Alexopoulou L; Blackshear PJ; Gaestel M; Davis R; Flavell R; Kollias G. 2001. Interleukin-10 targets p38 MAPK to modulate ARE-dependent TNF mRNA translation and limit intestinal pathology. EMBO J 20(14):3760-70. [PubMed: 11447117]  [MGI Ref ID J:108402]

Lim AK; Nikolic-Paterson DJ; Ma FY; Ozols E; Thomas MC; Flavell RA; Davis RJ; Tesch GH. 2009. Role of MKK3-p38 MAPK signalling in the development of type 2 diabetes and renal injury in obese db/db mice. Diabetologia 52(2):347-58. [PubMed: 19066844]  [MGI Ref ID J:144365]

Lim JH; Stirling B; Derry J; Koga T; Jono H; Woo CH; Xu H; Bourne P; Ha UH; Ishinaga H; Xu H; Andalibi A; Feng XH; Zhu H; Huang Y; Zhang W; Weng X; Yan C; Yin Z; Briles DE; Davis RJ; Flavell RA; Li JD. 2007. Tumor Suppressor CYLD Regulates Acute Lung Injury in Lethal Streptococcus pneumoniae Infections. Immunity 27(2):349-60. [PubMed: 17723219]  [MGI Ref ID J:124327]

Ma FY; Tesch GH; Flavell RA; Davis RJ; Nikolic-Paterson DJ. 2007. MKK3-p38 signaling promotes apoptosis and the early inflammatory response in the obstructed mouse kidney. Am J Physiol Renal Physiol 293(5):F1556-63. [PubMed: 17686961]  [MGI Ref ID J:145121]

MacNeil AJ; Junkins RD; Wu Z; Lin TJ. 2014. Stem cell factor induces AP-1-dependent mast cell IL-6 production via MAPK kinase 3 activity. J Leukoc Biol 95(6):903-15. [PubMed: 24453276]  [MGI Ref ID J:211932]

MacNeil AJ; Yang YJ; Lin TJ. 2011. MAPK kinase 3 specifically regulates Fc epsilonRI-mediated IL-4 production by mast cells. J Immunol 187(6):3374-82. [PubMed: 21841136]  [MGI Ref ID J:179249]

Mannam P; Shinn AS; Srivastava A; Neamu RF; Walker WE; Bohanon M; Merkel J; Kang MJ; Dela Cruz CS; Ahasic AM; Pisani MA; Trentalange M; West AP; Shadel GS; Elias JA; Lee PJ. 2014. MKK3 regulates mitochondrial biogenesis and mitophagy in sepsis-induced lung injury. Am J Physiol Lung Cell Mol Physiol 306(7):L604-19. [PubMed: 24487387]  [MGI Ref ID J:210185]

Mannam P; Zhang X; Shan P; Zhang Y; Shinn AS; Zhang Y; Lee PJ. 2013. Endothelial MKK3 is a critical mediator of lethal murine endotoxemia and acute lung injury. J Immunol 190(3):1264-75. [PubMed: 23275604]  [MGI Ref ID J:193033]

Mikkelsen SS; Jensen SB; Chiliveru S; Melchjorsen J; Julkunen I; Gaestel M; Arthur JS; Flavell RA; Ghosh S; Paludan SR. 2009. RIG-I-mediated activation of p38 MAPK is essential for viral induction of interferon and activation of dendritic cells: dependence on TRAF2 and TAK1. J Biol Chem 284(16):10774-82. [PubMed: 19224920]  [MGI Ref ID J:149257]

Nagaleekar VK; Sabio G; Aktan I; Chant A; Howe IW; Thornton TM; Benoit PJ; Davis RJ; Rincon M; Boyson JE. 2011. Translational Control of NKT Cell Cytokine Production by p38 MAPK. J Immunol 186(7):4140-6. [PubMed: 21368234]  [MGI Ref ID J:170817]

Nagelin MH; Srinivasan S; Nadler JL; Hedrick CC. 2009. Murine 12/15-lipoxygenase regulates ATP-binding cassette transporter G1 protein degradation through p38- and JNK2-dependent pathways. J Biol Chem 284(45):31303-14. [PubMed: 19713213]  [MGI Ref ID J:156345]

Otterbein LE; Otterbein SL; Ifedigbo E; Liu F; Morse DE; Fearns C; Ulevitch RJ; Knickelbein R; Flavell RA; Choi AM. 2003. MKK3 mitogen-activated protein kinase pathway mediates carbon monoxide-induced protection against oxidant-induced lung injury. Am J Pathol 163(6):2555-63. [PubMed: 14633627]  [MGI Ref ID J:86602]

Riffo-Vasquez Y; Coates AR; Page CP; Spina D. 2012. Mycobacterium tuberculosis chaperonin 60.1 inhibits leukocyte diapedesis in a murine model of allergic lung inflammation. Am J Respir Cell Mol Biol 47(2):245-52. [PubMed: 22447969]  [MGI Ref ID J:199771]

Seimon TA; Obstfeld A; Moore KJ; Golenbock DT; Tabas I. 2006. Combinatorial pattern recognition receptor signaling alters the balance of life and death in macrophages. Proc Natl Acad Sci U S A 103(52):19794-9. [PubMed: 17167049]  [MGI Ref ID J:118240]

Sorkin LS; Boyle DL; Hammaker D; Herman DS; Vail E; Firestein GS. 2009. MKK3, an upstream activator of p38, contributes to formalin phase 2 and late allodynia in mice. Neuroscience 162(2):462-71. [PubMed: 19427893]  [MGI Ref ID J:152926]

Tanaka N; Kamanaka M; Enslen H; Dong C; Wysk M; Davis RJ; Flavell RA. 2002. Differential involvement of p38 mitogen-activated protein kinase kinases MKK3 and MKK6 in T-cell apoptosis. EMBO Rep 3(8):785-91. [PubMed: 12151339]  [MGI Ref ID J:85266]

Wang XM; Kim HP; Nakahira K; Ryter SW; Choi AM. 2009. The heme oxygenase-1/carbon monoxide pathway suppresses TLR4 signaling by regulating the interaction of TLR4 with caveolin-1. J Immunol 182(6):3809-18. [PubMed: 19265160]  [MGI Ref ID J:145914]

Wang XM; Kim HP; Song R; Choi AM. 2006. Caveolin-1 confers antiinflammatory effects in murine macrophages via the MKK3/p38 MAPK pathway. Am J Respir Cell Mol Biol 34(4):434-42. [PubMed: 16357362]  [MGI Ref ID J:120188]

Wen HC; Avivar-Valderas A; Sosa MS; Girnius N; Farias EF; Davis RJ; Aguirre-Ghiso JA. 2011. p38alpha Signaling Induces Anoikis and Lumen Formation During Mammary Morphogenesis. Sci Signal 4(174):ra34. [PubMed: 21610252]  [MGI Ref ID J:185994]

Yang Z; Zhang X; Darrah PA; Mosser DM. 2010. The Regulation of Th1 Responses by the p38 MAPK. J Immunol 185(10):6205-13. [PubMed: 20937847]  [MGI Ref ID J:165637]

Yoshizawa T; Hammaker D; Boyle DL; Corr M; Flavell R; Davis R; Schett G; Firestein GS. 2009. Role of MAPK kinase 6 in arthritis: distinct mechanism of action in inflammation and cytokine expression. J Immunol 183(2):1360-7. [PubMed: 19561096]  [MGI Ref ID J:151663]

Zhang X; Shan P; Alam J; Davis RJ; Flavell RA; Lee PJ. 2003. Carbon monoxide modulates Fas/Fas ligand, caspases, and Bcl-2 family proteins via the p38alpha mitogen-activated protein kinase pathway during ischemia-reperfusion lung injury. J Biol Chem 278(24):22061-70. [PubMed: 12690100]  [MGI Ref ID J:211469]

Zuckerbraun BS; Billiar TR; Otterbein SL; Kim PK; Liu F; Choi AM; Bach FH; Otterbein LE. 2003. Carbon monoxide protects against liver failure through nitric oxide-induced heme oxygenase 1. J Exp Med 198(11):1707-16. [PubMed: 14657222]  [MGI Ref ID J:86806]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2525.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3283.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   Wild-type from the colony
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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