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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
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Description
Strain Information
Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System +/+ sibling x Hemizygote (Female x Male) 18-APR-08 Species laboratory mouse Generation N9F6 (19-AUG-09) Donating Investigator Bradford Lowell, Beth Israel Deaconess Medical Center Description
Hemizygous mice are viable and fertile. These mice express humanized Renilla Green Fluorescent Protein (hrGFP, Stratagene) under control of the mouse neuropeptide Y (Npy) promoter. As such, UV light-exposed transgenic brain tissues show GFP fluorescence patterns consistent with the (Npy) gene. The donating investigator reports that the hrGFP expressed from this transgene is more stable and resistant to signal fading compared to other GFP’s. These transgenic mice may be useful for studies of neurobiology, energy metabolism, obesity, seizures, and epilepsy.Development
A bacterial artificial chromosome (BAC) was modified by inserting a humanized Renilla GFP (hrGFP, Stratagene) sequence into the translational start site of the mouse neuropeptide Y (Npy) gene. Correctly transformed and targeted bacteria were used to isolate the transgenic construct (called "NPY-GFP"). This NPY-GFP transgene was used for pronuclear injection into fertilized one-cell stage FVB embryos. Transgenic offspring were bred to C57BL/6 mice producing offspring with germline transmission of the transgene (founder line 1). Transgenic mice were backcrossed to C57BL/6 mice for at least 8 generations prior to arrival at The Jackson Laboratory.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (225 strains)
004545 129S-Npytm1Rpa/J 008321 B6.Cg-Tg(Npy-MAPT/GFP*)1Rck/J 007585 B6;129S4-Npytm2Rpa/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
assigned by genotype
Tg(Npy-hrGFP)1Lowl/0
B6.FVB-Tg(Npy-hrGFP)1Lowl
- normal phenotype
- no abnormal phenotype detected (MGI Ref ID J:120993)
- hemizygotes are viable and fertile
- when exposed to UV light, brain tissues show GFP fluorescence in patterns consistent with the endogenous mouse Npy gene
This mouse can be used to support research in many areas including:
GFP relatedNeurobiology Research
Epilepsy
Fluorescent protein expression in neural tissue
Research Tools
Diabetes and Obesity Research
Fluorescent Proteins
Metabolism Research
Neurobiology Research
cell marker
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Npy-hrGFP)1Lowl | ||
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| Allele Name | transgene insertion 1, Bradford B Lowell | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | NPY-GFP; | ||
| Mutation Made By | Bradford Lowell, Beth Israel Deaconess Medical Center | ||
| Strain of Origin | FVB | ||
| Site of Expression | brain tissues in patterns consistent with the neuropeptide Y gene; humanized Renilla Green Fluorescent Protein (hrGFP) is more stable and resistant to signal fading compared to other GFP’s | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Promoter | Npy, neuropeptide Y, mouse, laboratory | ||
| Molecular Note | A bacterial artificial chromosome (BAC) was modified by inserting a humanized Renilla GFP (hrGFP, Stratagene) sequence into the translational start site of the mouse neuropeptide Y (Npy) gene. Correctly transformed and targeted bacteria were used to isolate the ~200 kb transgenic construct (called "NPY-GFP"). This NPY-GFP transgene was used for pronuclear injection into fertilized one-cell stage FVB embryos. Founder line 1 showing germline transmission was established. Brain tissues show GFP fluorescencewhen exposed to UV light. [MGI Ref ID J:120993] | ||
| Gene Symbol and Name | Tg(Npy-hrGFP)1Lowl, transgene insertion 1, Bradford B Lowell | ||
| Chromosome | UN | ||
| Gene Common Name(s) | NPY-GFP; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(Npy-hrGFP)1Lowl, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Lowell BB. 2007. Generation of transgenic reporter mice with umanized Renilla GFP under control of the NPY promoter MGI Direct Data Submission :. [MGI Ref ID J:120993]
Additional References
Tg(Npy-hrGFP)1Lowl relatedOlofsson LE; Pierce AA; Xu AW. 2009. Functional requirement of AgRP and NPY neurons in ovarian cycle-dependent regulation of food intake. Proc Natl Acad Sci U S A 106(37):15932-7. [PubMed: 19805233] [MGI Ref ID J:153242]
van den Pol AN. 2009. Brain trauma enhances transient cytomegalovirus invasion of the brain only in mice that are immunodeficient. J Virol 83(1):420-7. [PubMed: 18945784] [MGI Ref ID J:153392]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, hemizygous mice can be bred to wildtype siblings or C57BL/6J. Alternatively, transgenic mice may be bred together, although the phenotype of homozygous mice is not known (October 2006). Mating System +/+ sibling x Hemizygote (Female x Male) 18-APR-08 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $243.50 Female or Male Hemizygous for Tg(Npy-hrGFP)1Lowl
Pairs /Price (US dollars $) Pair Genotype $297.85 Hemizygous for Tg(Npy-hrGFP)1Lowl x Noncarrier $297.85 Noncarrier x Hemizygous for Tg(Npy-hrGFP)1Lowl
Additional Supply Details
| Pricing for International shipping destinations |
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Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $316.60 Female or Male Hemizygous for Tg(Npy-hrGFP)1Lowl
Pairs /Price (US dollars $) Pair Genotype $387.30 Hemizygous for Tg(Npy-hrGFP)1Lowl x Noncarrier $387.30 Noncarrier x Hemizygous for Tg(Npy-hrGFP)1Lowl
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
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Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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