Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation N11F4pN1 Generation Definitions   Donating Investigator Eva Eicher,   The Jackson Laboratory

Description
Heterozygous female and homozygous male mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Female homozygous mice are infertile due to absence of primordial follicles in the ovary. No gene product (mRNA) is detected by RNase protection analysis of total ovarian RNA. This mutant mouse strain may be useful in studies of female infertility.

Development
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt the transcription and translation start site sequence. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6N blastocysts. The resulting chimeric animals were crossed to CF-1 mice, and then backcrossed to B6Ei for at least 5 generations.

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI

- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested. Premature Ovarian Failure 6; POF6   (FIGLA)

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Figla^tm1Dean/Figla^tm1Dean

        involves: 129S1/Sv * 129X1/SvJ * C57BL/6

• endocrine/exocrine gland phenotype
• abnormal ovarian folliculogenesis   (MGI Ref ID J:64677)
• absent ovarian follicles
  • absence of primordial follicles   (MGI Ref ID J:64677)
• small ovary
  • 1/20 the size of wild-type   (MGI Ref ID J:64677)
• reproductive system phenotype
• abnormal ovarian folliculogenesis   (MGI Ref ID J:64677)
• absent ovarian follicles
  • absence of primordial follicles   (MGI Ref ID J:64677)
• decreased oocyte number
  • perinatal decrease in the number of oocytes   (MGI Ref ID J:64677)
• female infertility
  • females are infertile but female Mullerian structures are normal   (MGI Ref ID J:64677)
  • males are fertile and show no apparent morphological abnormalities   (MGI Ref ID J:64677)
• small ovary
  • 1/20 the size of wild-type   (MGI Ref ID J:64677)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Reproductive Biology Research
Fertility Defects

Research Tools
Reproductive Biology Research

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Figlatm1Dean
Allele Name		targeted mutation 1, Jurrien Dean
Allele Type		Targeted (knock-out)
Common Name(s)		Figalphatm1Nih; Figalphatm2Nih; Figalphatm;
Mutation Made By		Dr. Jurrien Dean,   NIH
Strain of Origin		(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
ES Cell Line Name		R1
ES Cell Line Strain		(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Gene Symbol and Name		Figla, folliculogenesis specific basic helix-loop-helix
Chromosome		6
Gene Common Name(s)		BHLHC8; FIG alpha; FIGALPHA; POF6; RGD1564754;
Molecular Note		A 0.8 kb genomic fragment containing the transcription and translation start sites was replaced with a neomycin selection cassette. RNAse protection assays on RNA derived from ovaries of female homozygous mice demonstrated that no detectable transcript was present. [MGI Ref ID J:64677]

Genotyping

Genotyping Information

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Soyal SM; Amleh A; Dean J. 2000. FIGalpha, a germ cell-specific transcription factor required for ovarian follicle formation. Development 127(21):4645-54. [PubMed: 11023867]  [MGI Ref ID J:64677]

Additional References

Figla^tm1Dean related

Choi Y; Rajkovic A. 2006. Genetics of early mammalian folliculogenesis. Cell Mol Life Sci 63(5):579-90. [PubMed: 16416028]  [MGI Ref ID J:108336]

Hu W; Gauthier L; Baibakov B; Jimenez-Movilla M; Dean J. 2010. FIGLA, a basic helix-loop-helix transcription factor, balances sexually dimorphic gene expression in postnatal oocytes. Mol Cell Biol 30(14):3661-71. [PubMed: 20479125]  [MGI Ref ID J:162655]

Joshi S; Davies H; Sims LP; Levy SE; Dean J. 2007. Ovarian gene expression in the absence of FIGLA, an oocyte-specific transcription factor. BMC Dev Biol 7:67. [PubMed: 17567914]  [MGI Ref ID J:125833]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, heterozygous females and homozygous males are bred. Homozygous females are infertile due to absence of primordial follicles in the ovary.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

See Terms of Use tab for General Terms and Conditions

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Terms of Use

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