Strain Name: |
B6.129S6(CBA)-Cebpatm1Dgt/J |
|---|---|
Stock Number: |
006447 |
Availability: | Repository- Live |
General Terms and Conditions |
| Former Name |
B6.129S6-Cebpatm1Dgt/J (Changed: 11-JUL-07
) |
|
B6.Cg-Cebpatm1Dgt/J (Changed: 29-JUN-07
) | |
| Genes & Alleles | Cebpa; Cebpatm1Dgt; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Donating Investigator Daniel Tenen, Beth Israel Deaconess Medical Center Generation N9PN1F1 (03-JUN-07) Strain Description
Mice carrying this C/EBPalpha "floxed" allele (C/EBPalphaF) are viable and fertile. The floxed allele functions similarly to the wildtype allele. In mice homozygous for C/EBPalphaF and expressing an interferon-inducible Cre recombinase (introduced by breeding to a cre-expressing strain; see Stock No. 003556), C/EBPalpha activity is disrupted, leading to defective myeloid cell development, increased hematopoietic stem cell repopulating activity, and significantly increased myeloblast population in the bone marrow compartment. In combination with an appropriate Cre transgenic strain, these mutant mice may be useful in studies of hematopoietic cell (e.g. myeloid and basophil progenitor cell) development and function, cancer (e.g. acute myeloid leukemia), and alveolar cell differentiation.Strain Development
A targeting vector was designed to flank the single Cebpa exon with loxP sites. A neomycin resistance cassette was also inserted between the exon and the downstream loxP site. The targeting vector was introduced into 129S6/SvEvTac-derived TC1 embryonic stem (ES) cells. Correctly targeted cells were injected into C57BL/6 blastocysts and implanted into foster mothers. Mutant offspring were backcrossed for 9 generations to C57BL/6 mice. Prior to arrival at The Jackson Laboratory, mutant mice were crossed with "Mx1-Cre" transgenic mice also backcrossed 9 generations to C57BL/6 mice (B6.CBA genetic background, see Stock No. 006230). Upon arrival, double mutant mice were bred to C57BL/6J and the resulting offspring carrying only the C/EBPalphaF floxed allele were selectively bred to establish this strain.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Cebpatm1Dgt | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Daniel G Tenen | ||
| Common Name(s) | C/EBPalphaF; | ||
| Mutation Made By | Daniel Tenen, Beth Israel Deaconess Medical Center | ||
| Strain of Origin | 129S6/SvEvTac | ||
| ES Cell Line Name | TC-1 | ||
| ES Cell Line Strain | 129S6/SvEvTac | ||
| Gene Symbol and Name | Cebpa, CCAAT/enhancer binding protein (C/EBP), alpha | ||
| Chromosome | 7 | ||
| Gene Common Name(s) | C/EBP alpha; C/EBP-alpha; C/ebpalpha; CCAAT/enhancer binding protein (C/EBP); CEBP; Cebp; | ||
| Molecular Note | LoxP sites were inserted to flank the single exon and a neo cassette inserted downstream of the exon. Western blot of whole-cell lysates of adult liver and total bone marrow cells showed that protein was present in mutants. [MGI Ref ID J:94682] | ||
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Cebpatm1Dgt
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, mice homozygous for the "floxed" mutation are bred together. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying Cebpatm1Dgt allele
006230 B6.Cg-Cebpatm1Dgt Tg(Mx1-cre)1Cgn/J View Strains carrying Cebpatm1Dgt (1 strain)
Additional Web Information
Congenic Nomenclature
Cre-lox or FLP-FRT Systems
Animal Health Reports
Room Number AX11
Research Applications
This mouse can be used to support research in many areas including:
Cancer Research
Increased Tumor Incidence (Leukemia)
Increased Tumor Incidence (Leukemia: lymphocytic)
Cell Biology Research
Transcriptional Regulation
Developmental Biology Research
Lymphoid Tissue Defects (hematopoietic defects)
Hematological Research
Hematopoietic Defects
Immunological Defects
Immunology and Inflammation Research
Lymphoid Tissue Defects (hematopoietic development)
Lymphoid Tissue Defects (myeloid hyperplasia)
Internal/Organ Research
Lymphoid Tissue Defects
Research Tools
Cancer Research (Leukemia)
Cancer Research (myeloma and hybridoma production)
Cre-lox System (loxP-flanked Sequences)
Hematological Research
References
Selected Reference(s)
Additional ReferencesZhang P; Iwasaki-Arai J; Iwasaki H; Fenyus ML; Dayaram T; Owens BM; Shigematsu H; Levantini E; Huettner CS; Lekstrom-Himes JA; Akashi K; Tenen DG. 2004. Enhancement of hematopoietic stem cell repopulating capacity and self-renewal in the absence of the transcription factor C/EBP alpha. Immunity 21(6):853-63. [PubMed: 15589173] [MGI Ref ID J:94682]
Price and Supply Information
| Strain Name: | B6.129S6(CBA)-Cebpatm1Dgt/J |
| Stock Number: | 006447 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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Contact Information
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