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| When these Sim1-Cre mice are bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the flanked sequences in Sim1-expressing tissues (including hypothalamus). As such, Sim1-Cre transgenic mice may be useful in studying body weight homeostasis, obesity, leptin metabolism, or as a reporter strain for Sim1-transcription factor activity. | |||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Noncarrier x Hemizygote (Female x Male) 25-MAR-08 Species laboratory mouse Generation N6F2 (18-DEC-08) Donating Investigator Bradford Lowell, Beth Israel Deaconess Medical Center Description
Hemizygous mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Transgene expression is observed in all areas that endogenously express Sim1, including paraventricular hypothalamus and other parts of the brain. When these Sim1-Cre mice are bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the flanked sequences in Sim1-expressing tissues (including hypothalamus). As such, Sim1-Cre transgenic mice may be useful in studying body weight homeostasis, obesity, leptin metabolism, or as a reporter strain for Sim1-transcription factor activity.In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Development
A bacterial artificial chromosome (BAC) was modified by inserting a Cre recombinase sequence into the translational start site of the mouse Sim1 gene (removing the first 31 basepairs of Sim1. Correctly transformed and targeted bacteria had the selection cassette transiently removed (leaving a single frt site downstream of the Cre recombinase). The resulting construct, called "Sim1-Cre," was used for pro-nuclear injection into fertilized one-cell stage FVB embryos. Transgenic offspring (founder line 1) were established and maintained on a mixed genetic background (C57BL/6, 129/SvJ, and FVB) prior to arrival at The Jackson Laboratory (as Stock No. 006395). Upon arrival, mice were backcrossed to C57BL/6J for at least 5 generations to establish this congenic strain.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying Tg(Sim1-cre)1Lowl allele
006395 STOCK Tg(Sim1-cre)1Lowl/J View Strains carrying Tg(Sim1-cre)1Lowl (1 strain)
007570 STOCK Sim1tm1.2Az/J
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
cre relatedEndocrine Deficiency Research
Hypothalamus/Pituitary Defects
Neurobiology Research
Cre-lox System
Cre Recombinase expression in neural tissue
Research Tools
Cre-lox System
Cre Recombinase Expression
Diabetes and Obesity Research
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Metabolism Research
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Sim1-cre)1Lowl | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Bradford B Lowell | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | Sim1-Cre; | ||
| Mutation Made By | Bradford Lowell, Beth Israel Deaconess Medical Center | ||
| Strain of Origin | FVB | ||
| Site of Expression | all areas that endogenously express Sim1, including paraventricular hypothalamus and other parts of the brain | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Sim1, single-minded homolog 1 (Drosophila), mouse, laboratory | ||
| Driver Note | Sim1 | ||
| Molecular Note | The cre ATG was inserted via recombineering techniques, into the Sim1 ATG site of a BAC containing 137.1 kb of genomic C57BL/6 Sim1 sequences up to and including exon 8. The inserted cre sequence deleted the first 31 bp of the Sim1 gene. [MGI Ref ID J:115192] | ||
| Gene Symbol and Name | Tg(Sim1-cre)1Lowl, transgene insertion 1, Bradford B Lowell | ||
| Chromosome | UN | ||
| Gene Common Name(s) | Sim1-Cre; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic Cre Melt Curve Analysis, Melt Curve Analysis
Generic Cre, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Balthasar N; Dalgaard LT; Lee CE; Yu J; Funahashi H; Williams T; Ferreira M; Tang V; McGovern RA; Kenny CD; Christiansen LM; Edelstein E; Choi B; Boss O; Aschkenasi C; Zhang CY; Mountjoy K; Kishi T; Elmquist JK; Lowell BB. 2005. Divergence of melanocortin pathways in the control of food intake and energy expenditure. Cell 123(3):493-505. [PubMed: 16269339] [MGI Ref ID J:115192]
Additional References
Tg(Sim1-cre)1Lowl relatedFyffe SL; Neul JL; Samaco RC; Chao HT; Ben-Shachar S; Moretti P; McGill BE; Goulding EH; Sullivan E; Tecott LH; Zoghbi HY. 2008. Deletion of Mecp2 in Sim1-expressing neurons reveals a critical role for MeCP2 in feeding behavior, aggression, and the response to stress. Neuron 59(6):947-58. [PubMed: 18817733] [MGI Ref ID J:143360]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, hemizygous mice are bred to C57BL/6J or wildtype siblings. Mating System Noncarrier x Hemizygote (Female x Male) 25-MAR-08 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $243.50 Female or Male Hemizygous for Tg(Sim1-cre)1Lowl
Pairs /Price (US dollars $) Pair Genotype $297.85 Hemizygous for Tg(Sim1-cre)1Lowl x Noncarrier $297.85 Noncarrier x Hemizygous for Tg(Sim1-cre)1Lowl
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $316.60 Female or Male Hemizygous for Tg(Sim1-cre)1Lowl
Pairs /Price (US dollars $) Pair Genotype $387.30 Hemizygous for Tg(Sim1-cre)1Lowl x Noncarrier $387.30 Noncarrier x Hemizygous for Tg(Sim1-cre)1Lowl
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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