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| These ZW mice harbor a Cre-conditional pCZW transgene and have widespread expression of lacZ under the control of the CMV enhancer/chicken actin promoter. When bred to mice expressing Cre recombinase, the "floxed" lacZ sequence is removed in the resulting offspring, and lacZ expression is replaced by wheat germ agglutinin (plant lectin) expression in all cre-expressing cells. These ZW mice may be useful for global expression of lacZ or, when crossed to a cre-expressing strain, for tracing transneuronal or trans-synaptic connections and circuits in brain regions or in the spinal cord. | |||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Former Names B6;CBA-Tg(ACTB-lacZ-WGA)330Bbm/J (Changed: 08-MAY-08 ) Type Mutant Stock; Transgenic; Additional information on Genetically Engineered Mutant Mice. Mating System +/+ sibling x Hemizygote (Female x Male) Species laboratory mouse Generation ?N1+F1 (03-JUN-07) Donating Investigator Allan Basbaum, Univ. California, San Francisco Description
These ZW transgenic mice constitutively express lacZ under the control of the CMV enhancer/chicken actin promoter. Expression is widespread, but mosaic, throughout the central and peripheral nervous systems. Purkinje cells display intense beta-galactosidase activity. Approximately 50% of the total neuron population express the transgene, as detected by beta-galactosidase activity. Newborn mice exhibit widespread beta-galactosidase activity. When crossed with a Cre recombinase-expressing strain, lacZ expression is replaced with wheat germ agglutinin (plant lectin) expression in tissues expressing cre. The double reporter system makes it possible to distinguish a lack of reporter (lacZ) expression from a lack of Cre recombinase expression while providing a means to assess Cre excision activity at the individual cell level. This transgenic mouse strain may be useful in tracing transneuronal or trans-synaptic connections and circuits in brain regions or in the spinal cord. Mice homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 100011 B6CBAF1/J | ||
| Considerations for Choosing Controls | ||
Related Strains
lacZ Expression Strains
View lacZ Expression Strains (175 strains)
006477 B6.Cg-Tg(CAG-lacZ-WGA)330Bbm/J
Additional Web Information
Cre-lox Systems
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
assigned by genotype
Tg(CAG-lacZ-WGA)330Bbm/Tg(CAG-lacZ-WGA)330Bbm
involves: C57BL/6 * CBA/J
- normal phenotype
- no abnormal phenotype detected (MGI Ref ID J:92042)
- mice homozygous for the transgene are viable and exhibit no physical or behavioral abnormalities
This mouse can be used to support research in many areas including:
lacZ relatedNeurobiology Research
lacZ expression in neural tissue
Cre-lox System (loxP-flanked Sequences: Test/Reporter)
Research Tools
lacZ Expression
Cell Biology Research
Cre-lox System (loxP-flanked Sequences: Test/Reporter)
Genetics Research (Tissue/Cell Markers: multiple)
Genetics Research (Tissue/Cell Markers: neurons)
Neurobiology Research (cell marker)
Research Tools
lacZ Expression
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(CAG-lacZ-WGA)330Bbm | ||
|---|---|---|---|
| Allele Name | transgene insertion 330, Allan Basbaum | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | Tg(ACTB-lacZ-WGA)330Bbm; Tg(CAG-lacZ-WGA)330Bbm; ZW; ZW19; | ||
| Mutation Made By | Joao Braz, University of California San Francisco | ||
| Strain of Origin | (C57BL/6J x CBA/J)F1 | ||
| Site of Expression | lacZ is expressed throughout central and peripheral nervous system; when crossed with a cre recombinase-expressing strain, lacZ expression is replaced with wheat germ agglutinin expression | ||
| Expressed Gene | WGA, Wheat Germ Agglutin, truncated, | ||
| Expressed Gene | lacZ, beta-galactosidase, E. coli | ||
| Promoter | ACTB, actin, beta, chicken | ||
| General Note | Of three transgenic lines generated - called ZW5, ZW16 and ZW19 by Braz and colleagues (2002) - only ZW19, which exhibited the highest transmission rates, was propagated. | ||
| Molecular Note | The transgene comprises a minimal immediate-early CMV enhancer/chicken beta-actin promoter driving ubiquitous expression of a lox-P flanked beta-galactosidase/neomycin phosphotransferase II (beta-geo) fusion gene that terminates in a strong, triple polyadenylation signal, followed by a C-terminally truncated wheat germ agglutinin coding sequence. Neuron-specific excision of the beta-geo gene by Cre recombinase permits expression of the lectin, whose migration allows tracing of synapses involving the expressing cells. [MGI Ref ID J:92042] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic LacZ Melt Curve Analysis, MCA, vers. 2
Generic LacZ, STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Braz JM; Rico B; Basbaum AI. 2002. Transneuronal tracing of diverse CNS circuits by Cre-mediated induction of wheat germ agglutinin in transgenic mice. Proc Natl Acad Sci U S A 99(23):15148-53. [PubMed: 12391304] [MGI Ref ID J:92042]
Additional References
Tg(CAG-lacZ-WGA)330Bbm relatedBraz JM; Nassar MA; Wood JN; Basbaum AI. 2005. Parallel 'pain' pathways arise from subpopulations of primary afferent nociceptor. Neuron 47(6):787-93. [PubMed: 16157274] [MGI Ref ID J:100993]
Kim IJ; Zhang Y; Yamagata M; Meister M; Sanes JR. 2008. Molecular identification of a retinal cell type that responds to upward motion. Nature 452(7186):478-82. [PubMed: 18368118] [MGI Ref ID J:134167]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, hemizygous mice are bred to wildtype siblings or to B6CBAF1/J (Stock No. 100011). Mating System +/+ sibling x Hemizygote (Female x Male) Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $203.80 Female or Male Hemizygous for Tg(CAG-lacZ-WGA)330Bbm *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $256.05 Hemizygous for Tg(CAG-lacZ-WGA)330Bbm x Noncarrier $256.05 Noncarrier x Hemizygous for Tg(CAG-lacZ-WGA)330Bbm
Additional Supply Details
| Supply Notes |
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| Pricing for International shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $265.00 Female or Male Hemizygous for Tg(CAG-lacZ-WGA)330Bbm *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $332.90 Hemizygous for Tg(CAG-lacZ-WGA)330Bbm x Noncarrier $332.90 Noncarrier x Hemizygous for Tg(CAG-lacZ-WGA)330Bbm
Additional Supply Details
| Supply Notes |
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 100011 B6CBAF1/J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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Contact information
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