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Strain Name:

B6;CBA-Tg(CAG-lacZ-WGA)330Bbm/J

Stock Number:

006465

Availability:

Repository- Live

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General Terms and Conditions
Additional Licensing and Use Restrictions

Former Name      B6;CBA-Tg(ACTB-lacZ-WGA)330Bbm/J    (Changed: 08-MAY-08 )
Genes & Alleles   ACTB;   WGA;   lacZ;   Tg(CAG-lacZ-WGA)330Bbm;

Product Information

Strain Details

Type JAX® GEMM® Strain - Mutant Stock
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Transgenic
Mating System+/+ sibling x Hemizygote         (Female x Male)
Specieslaboratory mouse
Donating Investigator Allan Basbaum,   Univ. California, San Francisco
Generation?N1+F1 (03-JUN-07)

Strain Description
These ZW transgenic mice constitutively express lacZ under the control of the CMV enhancer/chicken actin promoter. Expression is widespread, but mosaic, throughout the central and peripheral nervous systems. Purkinje cells display intense beta-galactosidase activity. Approximately 50% of the total neuron population express the transgene, as detected by beta-galactosidase activity. Newborn mice exhibit widespread beta-galactosidase activity. When crossed with a Cre recombinase-expressing strain, lacZ expression is replaced with wheat germ agglutinin (plant lectin) expression in tissues expressing cre. The double reporter system makes it possible to distinguish a lack of reporter (lacZ) expression from a lack of Cre recombinase expression while providing a means to assess Cre excision activity at the individual cell level. This transgenic mouse strain may be useful in tracing transneuronal or trans-synaptic connections and circuits in brain regions or in the spinal cord. Mice homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.

Mammalian Phenotype Terms assigned by genotype

Tg(CAG-lacZ-WGA)330Bbm/Tg(CAG-lacZ-WGA)330Bbm

        involves: C57BL/6 * CBA/J
  • normal phenotype
  • no abnormal phenotype detected (MGI Ref ID J:92042)
    • mice homozygous for the transgene are viable and exhibit no physical or behavioral abnormalities

Gene & Allele Details

Allele Symbol Tg(CAG-lacZ-WGA)330Bbm
Allele Name transgene insertion 330, Allan Basbaum
Common Name(s) Tg(ACTB-lacZ-WGA)330Bbm; Tg(CAG-lacZ-WGA)330Bbm; ZW; ZW19;
Mutation Made By Joao Braz,   University of California San Francisco
Strain of Origin(C57BL/6J x CBA/J)F1
Site of ExpressionlacZ is expressed throughout central and peripheral nervous system; when crossed with a cre recombinase-expressing strain, lacZ expression is replaced with wheat germ agglutinin expression
Expressed Gene WGA, Wheat Germ Agglutin, truncated,
Expressed Gene lacZ, beta-galactosidase, E. coli
Promoter ACTB, actin, beta, chicken
General Note Of three transgenic lines generated - called ZW5, ZW16 and ZW19 by Braz and colleagues (2002) - only ZW19, which exhibited the highest transmission rates, was propagated.
Molecular Note The transgene comprises a minimal immediate-early CMV enhancer/chicken beta-actin promoter driving ubiquitous expression of a lox-P flanked beta-galactosidase/neomycin phosphotransferase II (beta-geo) fusion gene that terminates in a strong, triple polyadenylation signal, followed by a C-terminally truncated wheat germ agglutinin coding sequence. Neuron-specific excision of the beta-geo gene by Cre recombinase permits expression of the lectin, whose migration allows tracing of synapses involving the expressing cells. [MGI Ref ID J:92042]

Control Information

  Control
   Noncarrier
   100011 B6CBAF1/J
 
  Considerations for Choosing Controls

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, hemizygous mice are bred to wildtype siblings or to B6CBAF1/J (Stock No. 100011).
Diet Information LabDiet® 5K52/5K67

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View lacZ Expression Strains     (174 strains)

Strains carrying   Tg(CAG-lacZ-WGA)330Bbm allele
006477   B6.Cg-Tg(CAG-lacZ-WGA)330Bbm/J
View Strains carrying   Tg(CAG-lacZ-WGA)330Bbm     (1 strain)

View Strains carrying other alleles of ACTB     (30 strains)

View Strains carrying other alleles of lacZ     (35 strains)

Additional Web Information

Cre-lox or FLP-FRT Systems
Fluorescent Proteins/lacZ Systems

Animal Health Reports

Room Number           AX11

Research Applications

This mouse can be used to support research in many areas including:

Neurobiology Research
lacZ expression in neural tissue

Research Tools
lacZ Expression
Cell Biology Research
Cre-lox System (loxP-flanked Sequences: Test/Reporter)
Genetics Research (Tissue/Cell Markers: multiple)
Genetics Research (Tissue/Cell Markers: neurons)
Neurobiology Research (cell marker)

lacZ related

Research Tools
lacZ Expression

References

Selected Reference(s)

Braz JM; Rico B; Basbaum AI. 2002. Transneuronal tracing of diverse CNS circuits by Cre-mediated induction of wheat germ agglutinin in transgenic mice. Proc Natl Acad Sci U S A 99(23):15148-53. [PubMed: 12391304]  [MGI Ref ID J:92042]

Additional References

Price and Supply Information

Strain Name: B6;CBA-Tg(CAG-lacZ-WGA)330Bbm/J
Stock Number: 006465

Price Details

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Supply Details

Standard SupplyRepository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes Usually shipped between four and eight weeks of age.
This strain is included in the Induced Mutant Resource Colony collection.
LicensingSee General Terms and Conditions below for Licensing and Use Restrictions  
Control InformationView Control Information in Strain Details.

General Terms and Conditions

View JAX® Mice & Services Conditions of Use.

Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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