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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Coisogenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System +/+ sibling x Hemizygote (Female x Male) 20-JUL-08 Species laboratory mouse Generation ?+F5 (18-NOV-09)
Generation DefinitionsDonating Investigator Susumu Tonegawa, Massachusetts Institute of Technology Description
Mice hemizygous for this "G32-4" transgene are viable, fertile, and do not display any gross physical or behavioral abnormalities. Transgene expression (cre activity) is detectable at 14 days old in area CA3 of the hippocampus, and at 8 weeks of age, recombination is observed in nearly 100% of pyramidal cells in area CA3. Recombination is also observed in other brain areas, but at distinctly lower frequencies. If bred with mice containing a loxP-flanked sequence of interest, tissue-specific deletion of that sequence results in the offspring. Specifically, when these mice were bred with a conditional CaMKII allele (see Stock No. 006575), the resulting offspring exhibited altered neurotransmitter release. The donating investigator reports that G32-4 females may confer global Cre-mediated deletion of loxP-flanked sequences in some offspring, and recommend using male G32-4 mice exclusively for such trials. These Cre-expressing mice may be useful in neurological studies involving hippocampus learning and memory, area CA3, and pyramidal cells.Development
The entire bacterial artificial chromosome (BAC1)-DNA fragment containing the mouse Grik4 (or KA-1) genomic sequence (including the putative promoter, exon 1 (~338 bp) and exon 2 (45 bp), but not exon 3 (105 bp) carrying the translation initiation codon) was coinjected into fertilized C57BL/6 mouse eggs along with a DNA construct carrying both the minimal heat shock promoter and the Cre-recombinase cDNA sequence with nuclear localization signal. Transgenic offspring in which both cre and BAC1 sequences cointegrated into the genome (founder line G32-4) were established and maintained on the C57BL/6 genetic background prior to arrival at The Jackson Laboratory.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of cre
View Strains carrying other alleles of cre (167 strains)
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
cre relatedNeurobiology Research
Behavioral and Learning Defects
Cre-lox System
Cre Recombinase expression in neural tissue
Research Tools
Cre-lox System
Cre Recombinase Expression
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Grik4-cre)G32-4Stl | ||
|---|---|---|---|
| Allele Name | transgene insertion G32-4, Susumu Tonegawa | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | G32-4 Cre; Grik4-Cre; KA1-Cre; | ||
| Mutation Made By | Susumu Tonegawa, Massachusetts Institute of Technology | ||
| Strain of Origin | C57BL/6 | ||
| Site of Expression | detectable at 14 days old in area CA3 of the hippocampus, and at 8 weeks of age, recombination is observed in nearly 100% of pyramidal cells in area CA3; recombination is also observed in other brain areas, but at distinctly lower frequencies | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Grik4, glutamate receptor, ionotropic, kainate 4, mouse, laboratory | ||
| Driver Note | Grik4 | ||
| Molecular Note | The transgenic constuct consisted of the Grik4 promoter and exons 1 and 2 adjoined to the cre recombinase coding region. The Grik4 promoter was shown to drive expression in the CA3 pyramidal cell layer by staining of a mouse also harboring a lacZ reporter transgene that is expressed upon cre mediated recombination. [MGI Ref ID J:77718] | ||
| Gene Symbol and Name | Tg(Grik4-cre)G32-4Stl, transgene insertion G32-4, Susumu Tonegawa | ||
| Chromosome | UN | ||
| Gene Common Name(s) | G32-4 Cre; Grik4-Cre; KA1-Cre; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic Cre Melt Curve Analysis, Fast MCA
Generic Cre Melt Curve Analysis, Melt Curve Analysis
Generic Cre, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Nakazawa K; Quirk MC; Chitwood RA; Watanabe M; Yeckel MF; Sun LD; Kato A; Carr CA; Johnston D; Wilson MA; Tonegawa S. 2002. Requirement for hippocampal CA3 NMDA receptors in associative memory recall. Science 297(5579):211-8. [PubMed: 12040087] [MGI Ref ID J:77718]
Additional References
Tg(Grik4-cre)G32-4Stl relatedCravens CJ; Vargas-Pinto N; Christian KM; Nakazawa K. 2006. CA3 NMDA receptors are crucial for rapid and automatic representation of context memory. Eur J Neurosci 24(6):1771-80. [PubMed: 17004940] [MGI Ref ID J:112944]
Hinds HL; Goussakov I; Nakazawa K; Tonegawa S; Bolshakov VY. 2003. Essential function of alpha-calcium/calmodulin-dependent protein kinase II in neurotransmitter release at a glutamatergic central synapse. Proc Natl Acad Sci U S A 100(7):4275-80. [PubMed: 12629219] [MGI Ref ID J:82744]
Jinde S; Belforte JE; Yamamoto J; Wilson MA; Tonegawa S; Nakazawa K. 2009. Lack of kainic acid-induced gamma oscillations predicts subsequent CA1 excitotoxic cell death. Eur J Neurosci 30(6):1036-55. [PubMed: 19735292] [MGI Ref ID J:153511]
Kamsler A; McHugh TJ; Gerber D; Huang SY; Tonegawa S. 2010. Presynaptic m1 muscarinic receptors are necessary for mGluR long-term depression in the hippocampus. Proc Natl Acad Sci U S A :. [PubMed: 20080609] [MGI Ref ID J:156540]
Nakashiba T; Buhl DL; McHugh TJ; Tonegawa S. 2009. Hippocampal CA3 output is crucial for ripple-associated reactivation and consolidation of memory. Neuron 62(6):781-7. [PubMed: 19555647] [MGI Ref ID J:157339]
Nakashiba T; Young JZ; McHugh TJ; Buhl DL; Tonegawa S. 2008. Transgenic inhibition of synaptic transmission reveals role of CA3 output in hippocampal learning. Science 319(5867):1260-4. [PubMed: 18218862] [MGI Ref ID J:131852]
Nakazawa K; Sun LD; Quirk MC; Rondi-Reig L; Wilson MA; Tonegawa S. 2003. Hippocampal CA3 NMDA receptors are crucial for memory acquisition of one-time experience. Neuron 38(2):305-15. [PubMed: 12718863] [MGI Ref ID J:107692]
Zhang C; Wu B; Beglopoulos V; Wines-Samuelson M; Zhang D; Dragatsis I; Sudhof TC; Shen J. 2009. Presenilins are essential for regulating neurotransmitter release. Nature 460(7255):632-6. [PubMed: 19641596] [MGI Ref ID J:150936]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, hemizygous mice are bred together, to wildtype siblings, or to C57BL/6J inbred mice. Mating System +/+ sibling x Hemizygote (Female x Male) 20-JUL-08 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $243.50 Female or Male Hemizygous for Tg(Grik4-cre)G32-4Stl
Pairs /Price (US dollars $) Pair Genotype $297.85 Hemizygous for Tg(Grik4-cre)G32-4Stl x Noncarrier $297.85 Noncarrier x Hemizygous for Tg(Grik4-cre)G32-4Stl Standard Supply
Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and/or larger quantities of mice may be possible. Estimated ship dates for all orders provided within two business days following order placement.
Supply Notes
- Usually shipped between four and eight weeks of age.
| Pricing for International shipping destinations |
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Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $316.60 Female or Male Hemizygous for Tg(Grik4-cre)G32-4Stl
Pairs /Price (US dollars $) Pair Genotype $387.30 Hemizygous for Tg(Grik4-cre)G32-4Stl x Noncarrier $387.30 Noncarrier x Hemizygous for Tg(Grik4-cre)G32-4Stl Standard Supply
Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and/or larger quantities of mice may be possible. Estimated ship dates for all orders provided within two business days following order placement.
Supply Notes
- Usually shipped between four and eight weeks of age.
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|
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Standard Supply
Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and/or larger quantities of mice may be possible. Estimated ship dates for all orders provided within two business days following order placement.
General Supply Notes
- This strain is included in the Induced Mutant Resource Colony collection.
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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