Strain Name: |
C57BL/6-Tg(Grik4-cre)G32-4Stl/J |
|---|---|
Stock Number: |
006474 |
Availability: | Repository- Live |
Price and Supply Information | |
General Terms and Conditions |
| Genes & Alleles | Grik4; cre; Tg(Grik4-cre)G32-4Stl; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Coisogenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Mating System +/+ sibling x Hemizygote (Female x Male) Species laboratory mouse Donating Investigator Susumu Tonegawa, Massachusetts Institute of Technology Generation ?+F0 (28-DEC-07) Strain Description
Mice hemizygous for this "G32-4" transgene are viable, fertile, and do not display any gross physical or behavioral abnormalities. Transgene expression (cre activity) is detectable at 14 days old in area CA3 of the hippocampus, and at 8 weeks of age, recombination is observed in nearly 100% of pyramidal cells in area CA3. Recombination is also observed in other brain areas, but at distinctly lower frequencies. If bred with mice containing a loxP-flanked sequence of interest, tissue-specific deletion of that sequence results in the offspring. Specifically, when these mice were bred with a conditional CaMKII allele (see Stock No. 006575), the resulting offspring exhibited altered neurotransmitter release. The donating investigator reports that G32-4 females may confer global Cre-mediated deletion of loxP-flanked sequences in some offspring, and recommend using male G32-4 mice exclusively for such trials. These Cre-expressing mice may be useful in neurological studies involving hippocampus learning and memory, area CA3, and pyramidal cells.Strain Development
The entire bacterial artificial chromosome (BAC1)-DNA fragment containing the mouse Grik4 (or KA-1) genomic sequence (including the putative promoter, exon 1 (~338 bp) and exon 2 (45 bp), but not exon 3 (105 bp) carrying the translation initiation codon) was coinjected into fertilized C57BL/6 mouse eggs along with a DNA construct carrying both the minimal heat shock promoter and the Cre-recombinase cDNA sequence with nuclear localization signal. Transgenic offspring in which both cre and BAC1 sequences cointegrated into the genome (founder line G32-4) were established and maintained on the C57BL/6 genetic background prior to arrival at The Jackson Laboratory.
Gene & Allele Details
| Allele Symbol | Tg(Grik4-cre)G32-4Stl | ||
|---|---|---|---|
| Allele Name | transgene insertion G32-4, Susumu Tonegawa | ||
| Common Name(s) | G32-4 Cre; KA1-Cre; | ||
| Mutation Made By | Susumu Tonegawa, Massachusetts Institute of Technology | ||
| Strain of Origin | C57BL/6 | ||
| Site of Expression | detectable at 14 days old in area CA3 of the hippocampus, and at 8 weeks of age, recombination is observed in nearly 100% of pyramidal cells in area CA3; recombination is also observed in other brain areas, but at distinctly lower frequencies | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Grik4, glutamate receptor, ionotropic, kainate 4, mouse, laboratory | ||
| Molecular Note | The transgenic constuct consisted of the Grik4 promoter and exons 1 and 2 adjoined to the cre recombinase coding region. The Grik4 promoter was shown to drive expression in the CA3 pyramidal cell layer by staining of a mouse also harboring a lacZ reporter transgene that is expressed upon cre mediated recombination. [MGI Ref ID J:77718] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for JAX® GEMM® Strains | ||
Genotyping Protocols
Generic Cre
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, hemizygous mice are bred together, to wildtype siblings, or to C57BL/6J inbred mice. |
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| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying other alleles of cre
View Strains carrying other alleles of cre (116 strains)
Additional Web Information
Cre-lox or FLP-FRT Systems
Animal Health Reports
Room Number AX11
Research Applications
This mouse can be used to support research in many areas including:
cre relatedNeurobiology Research
Behavioral and Learning Defects
Cre-lox System (Cre Recombinase expression in neural tissue)
Research Tools
Cre-lox System (Cre Recombinase Expression)
Research Tools
Cre-lox System
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
References
Selected Reference(s)
Additional ReferencesNakazawa K; Quirk MC; Chitwood RA; Watanabe M; Yeckel MF; Sun LD; Kato A; Carr CA; Johnston D; Wilson MA; Tonegawa S. 2002. Requirement for hippocampal CA3 NMDA receptors in associative memory recall. Science 297(5579):211-8. [PubMed: 12040087] [MGI Ref ID J:77718]
Price and Supply Information
| Strain Name: | C57BL/6-Tg(Grik4-cre)G32-4Stl/J |
| Stock Number: | 006474 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. The shipping destinations are:
- USA, Canada and Mexico
- International
*Pricing for Shipping Destination selected:
USA, Canada and Mexico
| Price(s) in US dollars ($) | Genotype(s) Provided | ||||
|---|---|---|---|---|---|
| Individual Mouse Price | $236.40 | Hemizygous for Tg(Grik4-cre)G32-4Stl | |||
| Pair | $288.65 | Hemizygous for Tg(Grik4-cre)G32-4Stl x Noncarrier | |||
| Pair | $288.65 | Noncarrier x Hemizygous for Tg(Grik4-cre)G32-4Stl | |||
Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
Histology and Tissue Collection Services are available for all JAX® Mice strains. For more information, please contact Customer Service at orderquest@jax.org or 1-800-422-6423. Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. View Control Pricing Information for JAX® Strains. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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