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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation N12+F2 (05-FEB-09) Donating Investigator C. Ronald Kahn, Joslin Diabetes Center Description
Hemizygous mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. These transgenic mice have the Cre recombinase gene driven by the muscle creatine kinase (MCK or Ckm) promoter. Cre activity is observed in skeletal and cardiac muscle. When bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in skeletal and cardiac muscle deletion of the flanked genome.Development
A transgene was designed with a cre recombinase cDNA sequence (with a SV-40 large T antigen nuclear localization signal and poly(A) signal) inserted in place of the translation initiation site of the Ckm gene. This construct was injected into fertilized FVB embryos which were then implanted into CD1 foster mothers. Chimeric mice were bred to FVB inbred animals to establish transgenic offspring (founder line 5). At some point, mice were bred to insulin receptor mutant mice on a mixed B6;129S4 genetic background. The double mutants were backcrossed for 10 generations to C57BL/6 mice and then selected for the transgene (and against the targeted mutation) prior to arrival at The Jackson Laboratory.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying Tg(Ckmm-cre)5Khn allele
006405 FVB-Tg(Ckmm-cre)5Khn/J View Strains carrying Tg(Ckmm-cre)5Khn (1 strain)
009651 B6.Cg-Sgcatm1Kcam Tg(Ckm-SGCE)1Kcam/J 006781 C57BL/6-Tg(Ckm-DGAT2)10Far/J 008231 C57BL/6-Tg(Ckm-Ppargc1a)31Brsp/J 008737 FVB-Tg(Ckm-Ppargc1b)T37Brsp/J
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cardiovascular Research
Cre-lox System
Cre-lox System
Cre Recombinase Expression
Diabetes and Obesity Research
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Ckmm-cre)5Khn | ||
|---|---|---|---|
| Allele Name | transgene insertion 5, C Ronald Kahn | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | Ckmm-NLS-cre; MCK-cre; MCK-cre5; MCKCre+; Tg(Ckmm-cre)1Khn; mckCRE; | ||
| Mutation Made By | C. Ronald Kahn, Joslin Diabetes Center | ||
| Strain of Origin | FVB | ||
| Site of Expression | skeletal and cardiac muscle | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Ckm, creatine kinase, muscle, mouse, laboratory | ||
| Driver Note | Ckmm | ||
| Molecular Note | This transgene expresses Cre recombinase under the control of a muscle creatine kinase promoter, which directs expression in the heart and skeletal muscle. [MGI Ref ID J:51266] | ||
| Gene Symbol and Name | Tg(Ckmm-cre)5Khn, transgene insertion 5, C Ronald Kahn | ||
| Chromosome | UN | ||
| Gene Common Name(s) | Ckmm-NLS-cre; MCK-Cre; MCK-cre5; MCKCre+; Tg(Ckmm-cre)1Khn; mckCRE; transgene insertion 1, C Ronald Kahn; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(Ckmm-cre)5Khn, Melt Curve Analysis
Tg(Ckmm-cre)5Khn, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Bruning JC; Michael MD; Winnay JN; Hayashi T; Horsch D; Accili D; Goodyear LJ; Kahn CR. 1998. A muscle-specific insulin receptor knockout exhibits features of the metabolic syndrome of NIDDM without altering glucose tolerance. Mol Cell 2(5):559-69. [PubMed: 9844629] [MGI Ref ID J:51266]
Additional References
Tg(Ckmm-cre)5Khn relatedAndrechek ER; Hardy WR; Girgis-Gabardo AA; Perry RL; Butler R; Graham FL; Kahn RC; Rudnicki MA; Muller WJ. 2002. ErbB2 is required for muscle spindle and myoblast cell survival. Mol Cell Biol 22(13):4714-22. [PubMed: 12052879] [MGI Ref ID J:81565]
Bayascas JR; Sakamoto K; Armit L; Arthur JS; Alessi DR. 2006. Evaluation of approaches to generation of tissue-specific knock-in mice. J Biol Chem 281(39):28772-81. [PubMed: 16887794] [MGI Ref ID J:117278]
Beedle AM; Nienaber PM; Campbell KP. 2007. Fukutin-related protein associates with the sarcolemmal dystrophin-glycoprotein complex. J Biol Chem 282(23):16713-7. [PubMed: 17452335] [MGI Ref ID J:122734]
Bence KK; Delibegovic M; Xue B; Gorgun CZ; Hotamisligil GS; Neel BG; Kahn BB. 2006. Neuronal PTP1B regulates body weight, adiposity and leptin action. Nat Med 12(8):917-24. [PubMed: 16845389] [MGI Ref ID J:111969]
Burcelin R; Crivelli V; Perrin C; Da Costa A; Mu J; Kahn BB; Birnbaum MJ; Kahn CR; Vollenweider P; Thorens B. 2003. GLUT4, AMP kinase, but not the insulin receptor, are required for hepatoportal glucose sensor-stimulated muscle glucose utilization. J Clin Invest 111(10):1555-62. [PubMed: 12750405] [MGI Ref ID J:134630]
Cariou B; Postic C; Boudou P; Burcelin R; Kahn CR; Girard J; Burnol AF; Mauvais-Jarvis F. 2004. Cellular and molecular mechanisms of adipose tissue plasticity in muscle insulin receptor knockout mice. Endocrinology 145(4):1926-32. [PubMed: 14684612] [MGI Ref ID J:88697]
Carvalho E; Kotani K; Peroni OD; Kahn BB. 2005. Adipose-specific overexpression of GLUT4 reverses insulin resistance and diabetes in mice lacking GLUT4 selectively in muscle. Am J Physiol Endocrinol Metab 289(4):E551-61. [PubMed: 15928024] [MGI Ref ID J:101261]
Cohen SE; Kokkotou E; Biddinger SB; Kondo T; Gebhardt R; Kratzsch J; Mantzoros CS; Kahn CR. 2007. High circulating leptin receptors with normal leptin sensitivity in liver-specific insulin receptor knock-out (LIRKO) mice. J Biol Chem 282(32):23672-8. [PubMed: 17556363] [MGI Ref ID J:124577]
Cohn RD; Henry MD; Michele DE; Barresi R; Saito F; Moore SA; Flanagan JD; Skwarchuk MW; Robbins ME; Mendell JR; Williamson RA; Campbell KP. 2002. Disruption of DAG1 in differentiated skeletal muscle reveals a role for dystroglycan in muscle regeneration. Cell 110(5):639-48. [PubMed: 12230980] [MGI Ref ID J:78838]
Crackower MA; Oudit GY; Kozieradzki I; Sarao R; Sun H; Sasaki T; Hirsch E; Suzuki A; Shioi T; Irie-Sasaki J; Sah R; Cheng HY; Rybin VO; Lembo G; Fratta L; Oliveira-dos-Santos AJ; Benovic JL; Kahn CR; Izumo S; Steinberg SF; Wymann MP; Backx PH; Penninger JM. 2002. Regulation of myocardial contractility and cell size by distinct PI3K-PTEN signaling pathways. Cell 110(6):737-49. [PubMed: 12297047] [MGI Ref ID J:79151]
Delibegovic M; Bence KK; Mody N; Hong EG; Ko HJ; Kim JK; Kahn BB; Neel BG. 2007. Improved glucose homeostasis in mice with muscle-specific deletion of protein-tyrosine phosphatase 1B. Mol Cell Biol 27(21):7727-34. [PubMed: 17724080] [MGI Ref ID J:129081]
Duvezin-Caubet S; Jagasia R; Wagener J; Hofmann S; Trifunovic A; Hansson A; Chomyn A; Bauer MF; Attardi G; Larsson NG; Neupert W; Reichert AS. 2006. Proteolytic processing of OPA1 links mitochondrial dysfunction to alterations in mitochondrial morphology. J Biol Chem 281(49):37972-9. [PubMed: 17003040] [MGI Ref ID J:117614]
Ealey KN; Lu S; Lau D; Archer MC. 2008. Reduced susceptibility of muscle-specific insulin receptor knockout mice to colon carcinogenesis. Am J Physiol Gastrointest Liver Physiol 294(3):G679-86. [PubMed: 18174274] [MGI Ref ID J:132394]
Farese RV; Sajan MP; Yang H; Li P; Mastorides S; Gower WR Jr; Nimal S; Choi CS; Kim S; Shulman GI; Kahn CR; Braun U; Leitges M. 2007. Muscle-specific knockout of PKC-lambda impairs glucose transport and induces metabolic and diabetic syndromes. J Clin Invest 117(8):2289-301. [PubMed: 17641777] [MGI Ref ID J:123964]
Fornaro M; Burch PM; Yang W; Zhang L; Hamilton CE; Kim JH; Neel BG; Bennett AM. 2006. SHP-2 activates signaling of the nuclear factor of activated T cells to promote skeletal muscle growth. J Cell Biol 175(1):87-97. [PubMed: 17015617] [MGI Ref ID J:114499]
Gilson H; Schakman O; Combaret L; Lause P; Grobet L; Attaix D; Ketelslegers JM; Thissen JP. 2007. Myostatin gene deletion prevents glucocorticoid-induced muscle atrophy. Endocrinology 148(1):452-60. [PubMed: 17038559] [MGI Ref ID J:129558]
Gilson H; Schakman O; Kalista S; Lause P; Tsuchida K; Thissen JP. 2009. Follistatin induces muscle hypertrophy through satellite cell proliferation and inhibition of both myostatin and activin. Am J Physiol Endocrinol Metab 297(1):E157-64. [PubMed: 19435857] [MGI Ref ID J:151164]
Goransson O; McBride A; Hawley SA; Ross FA; Shpiro N; Foretz M; Viollet B; Hardie DG; Sakamoto K. 2007. Mechanism of action of A-769662, a valuable tool for activation of AMP-activated protein kinase. J Biol Chem 282(45):32549-60. [PubMed: 17855357] [MGI Ref ID J:126943]
Gotthardt M; Hammer RE; Hubner N; Monti J; Witt CC; McNabb M; Richardson JA; Granzier H; Labeit S; Herz J. 2003. Conditional expression of mutant M-line titins results in cardiomyopathy with altered sarcomere structure. J Biol Chem 278(8):6059-65. [PubMed: 12464612] [MGI Ref ID J:81993]
Grobet L; Pirottin D; Farnir F; Poncelet D; Royo LJ; Brouwers B; Christians E; Desmecht D; Coignoul F; Kahn R; Georges M. 2003. Modulating skeletal muscle mass by postnatal, muscle-specific inactivation of the myostatin gene. Genesis 35(4):227-38. [PubMed: 12717734] [MGI Ref ID J:83122]
Habets DD; Coumans WA; El Hasnaoui M; Zarrinpashneh E; Bertrand L; Viollet B; Kiens B; Jensen TE; Richter EA; Bonen A; Glatz JF; Luiken JJ. 2009. Crucial role for LKB1 to AMPKalpha2 axis in the regulation of CD36-mediated long-chain fatty acid uptake into cardiomyocytes. Biochim Biophys Acta 1791(3):212-9. [PubMed: 19159696] [MGI Ref ID J:148739]
Han R; Kanagawa M; Yoshida-Moriguchi T; Rader EP; Ng RA; Michele DE; Muirhead DE; Kunz S; Moore SA; Iannaccone ST; Miyake K; McNeil PL; Mayer U; Oldstone MB; Faulkner JA; Campbell KP. 2009. Basal lamina strengthens cell membrane integrity via the laminin G domain-binding motif of alpha-dystroglycan. Proc Natl Acad Sci U S A 106(31):12573-9. [PubMed: 19633189] [MGI Ref ID J:152005]
Hansson A; Hance N; Dufour E; Rantanen A; Hultenby K; Clayton DA; Wibom R; Larsson NG. 2004. A switch in metabolism precedes increased mitochondrial biogenesis in respiratory chain-deficient mouse hearts. Proc Natl Acad Sci U S A 101(9):3136-41. [PubMed: 14978272] [MGI Ref ID J:88652]
Herrmann S; Stieber J; Stockl G; Hofmann F; Ludwig A. 2007. HCN4 provides a 'depolarization reserve' and is not required for heart rate acceleration in mice. EMBO J 26(21):4423-32. [PubMed: 17914461] [MGI Ref ID J:139560]
Hesser BA; Henschel O; Witzemann V. 2006. Synapse disassembly and formation of new synapses in postnatal muscle upon conditional inactivation of MuSK. Mol Cell Neurosci 31(3):470-80. [PubMed: 16337809] [MGI Ref ID J:106867]
Hevener AL; He W; Barak Y; Le J; Bandyopadhyay G; Olson P; Wilkes J; Evans RM; Olefsky J. 2003. Muscle-specific Pparg deletion causes insulin resistance. Nat Med 9(12):1491-7. [PubMed: 14625542] [MGI Ref ID J:86773]
Hu P; Zhang D; Swenson L; Chakrabarti G; Abel ED; Litwin SE. 2003. Minimally invasive aortic banding in mice: effects of altered cardiomyocyte insulin signaling during pressure overload. Am J Physiol Heart Circ Physiol 285(3):H1261-9. [PubMed: 12738623] [MGI Ref ID J:85401]
Hui ST; Andres AM; Miller AK; Spann NJ; Potter DW; Post NM; Chen AZ; Sachithanantham S; Jung DY; Kim JK; Davis RA. 2008. Txnip balances metabolic and growth signaling via PTEN disulfide reduction. Proc Natl Acad Sci U S A 105(10):3921-6. [PubMed: 18322014] [MGI Ref ID J:132756]
Joza N; Oudit GY; Brown D; Benit P; Kassiri Z; Vahsen N; Benoit L; Patel MM; Nowikovsky K; Vassault A; Backx PH; Wada T; Kroemer G; Rustin P; Penninger JM. 2005. Muscle-specific loss of apoptosis-inducing factor leads to mitochondrial dysfunction, skeletal muscle atrophy, and dilated cardiomyopathy. Mol Cell Biol 25(23):10261-72. [PubMed: 16287843] [MGI Ref ID J:113016]
Kim JK; Michael MD; Previs SF; Peroni OD; Mauvais-Jarvis F; Neschen S; Kahn BB; Kahn CR; Shulman GI. 2000. Redistribution of substrates to adipose tissue promotes obesity in mice with selective insulin resistance in muscle. J Clin Invest 105(12):1791-7. [PubMed: 10862794] [MGI Ref ID J:120531]
Kim JK; Zisman A; Fillmore JJ; Peroni OD; Kotani K; Perret P; Zong H; Dong J; Kahn CR; Kahn BB; Shulman GI. 2001. Glucose toxicity and the development of diabetes in mice with muscle-specific inactivation of GLUT4. J Clin Invest 108(1):153-60. [PubMed: 11435467] [MGI Ref ID J:110773]
Kleinridders A; Pogoda HM; Irlenbusch S; Smyth N; Koncz C; Hammerschmidt M; Bruning JC. 2009. PLRG1 is an essential regulator of cell proliferation and apoptosis during vertebrate development and tissue homeostasis. Mol Cell Biol 29(11):3173-85. [PubMed: 19307306] [MGI Ref ID J:149153]
Knauf C; Cani PD; Perrin C; Iglesias MA; Maury JF; Bernard E; Benhamed F; Gremeaux T; Drucker DJ; Kahn CR; Girard J; Tanti JF; Delzenne NM; Postic C; Burcelin R. 2005. Brain glucagon-like peptide-1 increases insulin secretion and muscle insulin resistance to favor hepatic glycogen storage. J Clin Invest 115(12):3554-63. [PubMed: 16322793] [MGI Ref ID J:104705]
Kobuke K; Piccolo F; Garringer KW; Moore SA; Sweezer E; Yang B; Campbell KP. 2008. A Common Disease-Associated Missense Mutation in Alpha-Sarcoglycan Fails to Cause Muscular Dystrophy in Mice. Hum Mol Genet :. [PubMed: 18252746] [MGI Ref ID J:130252]
Koh HJ; Arnolds DE; Fujii N; Tran TT; Rogers MJ; Jessen N; Li Y; Liew CW; Ho RC; Hirshman MF; Kulkarni RN; Kahn CR; Goodyear LJ. 2006. Skeletal Muscle-Selective Knockout of LKB1 Increases Insulin Sensitivity, Improves Glucose Homeostasis, and Decreases TRB3. Mol Cell Biol 26(22):8217-27. [PubMed: 16966378] [MGI Ref ID J:114640]
Konieczny P; Fuchs P; Reipert S; Kunz WS; Zeold A; Fischer I; Paulin D; Schroder R; Wiche G. 2008. Myofiber integrity depends on desmin network targeting to Z-disks and costameres via distinct plectin isoforms. J Cell Biol 181(4):667-81. [PubMed: 18490514] [MGI Ref ID J:137067]
Kontaridis MI; Yang W; Bence KK; Cullen D; Wang B; Bodyak N; Ke Q; Hinek A; Kang PM; Liao R; Neel BG. 2008. Deletion of Ptpn11 (Shp2) in cardiomyocytes causes dilated cardiomyopathy via effects on the extracellular signal-regulated kinase/mitogen-activated protein kinase and RhoA signaling pathways. Circulation 117(11):1423-35. [PubMed: 18316486] [MGI Ref ID J:148445]
Kotani K; Peroni OD; Minokoshi Y; Boss O; Kahn BB. 2004. GLUT4 glucose transporter deficiency increases hepatic lipid production and peripheral lipid utilization. J Clin Invest 114(11):1666-75. [PubMed: 15578099] [MGI Ref ID J:94432]
Laustsen PG; Russell SJ; Cui L; Entingh-Pearsall A; Holzenberger M; Liao R; Kahn CR. 2007. Essential role of insulin and insulin-like growth factor 1 receptor signaling in cardiac development and function. Mol Cell Biol 27(5):1649-64. [PubMed: 17189427] [MGI Ref ID J:118987]
Li S; Czubryt MP; McAnally J; Bassel-Duby R; Richardson JA; Wiebel FF; Nordheim A; Olson EN. 2005. Requirement for serum response factor for skeletal muscle growth and maturation revealed by tissue-specific gene deletion in mice. Proc Natl Acad Sci U S A 102(4):1082-7. [PubMed: 15647354] [MGI Ref ID J:96122]
Luo J; McMullen JR; Sobkiw CL; Zhang L; Dorfman AL; Sherwood MC; Logsdon MN; Horner JW; DePinho RA; Izumo S; Cantley LC. 2005. Class IA phosphoinositide 3-kinase regulates heart size and physiological cardiac hypertrophy. Mol Cell Biol 25(21):9491-502. [PubMed: 16227599] [MGI Ref ID J:102176]
Luo J; Sobkiw CL; Hirshman MF; Logsdon MN; Li TQ; Goodyear LJ; Cantley LC. 2006. Loss of class IA PI3K signaling in muscle leads to impaired muscle growth, insulin response, and hyperlipidemia. Cell Metab 3(5):355-66. [PubMed: 16679293] [MGI Ref ID J:129646]
MacLean HE; Chiu WS; Ma C; McManus JF; Davey RA; Cameron R; Notini AJ; Zajac JD. 2008. A floxed allele of the androgen receptor gene causes hyperandrogenization in male mice. Physiol Genomics 33(1):133-7. [PubMed: 18171720] [MGI Ref ID J:145319]
Mason SD; Howlett RA; Kim MJ; Olfert IM; Hogan MC; McNulty W; Hickey RP; Wagner PD; Kahn CR; Giordano FJ; Johnson RS. 2004. Loss of skeletal muscle HIF-1alpha results in altered exercise endurance. PLoS Biol 2(10):e288. [PubMed: 15328538] [MGI Ref ID J:97761]
Mason SD; Rundqvist H; Papandreou I; Duh R; McNulty WJ; Howlett RA; Olfert IM; Sundberg CJ; Denko NC; Poellinger L; Johnson RS. 2007. HIF-1alpha in endurance training: suppression of oxidative metabolism. Am J Physiol Regul Integr Comp Physiol 293(5):R2059-69. [PubMed: 17855495] [MGI Ref ID J:145111]
Mauvais-Jarvis F; Virkamaki A; Michael MD; Winnay JN; Zisman A; Kulkarni RN; Kahn CR. 2000. A model to explore the interaction between muscle insulin resistance and beta-cell dysfunction in the development of type 2 diabetes. Diabetes 49(12):2126-34. [PubMed: 11118016] [MGI Ref ID J:66009]
McGee SL; Mustard KJ; Hardie DG; Baar K. 2008. Normal hypertrophy accompanied by phosphoryation and activation of AMP-activated protein kinase alpha1 following overload in LKB1 knockout mice. J Physiol 586(6):1731-41. [PubMed: 18202101] [MGI Ref ID J:141208]
Metodiev MD; Lesko N; Park CB; Camara Y; Shi Y; Wibom R; Hultenby K; Gustafsson CM; Larsson NG. 2009. Methylation of 12S rRNA is necessary for in vivo stability of the small subunit of the mammalian mitochondrial ribosome. Cell Metab 9(4):386-97. [PubMed: 19356719] [MGI Ref ID J:148166]
Mora A; Davies AM; Bertrand L; Sharif I; Budas GR; Jovanovic S; Mouton V; Kahn CR; Lucocq JM; Gray GA; Jovanovic A; Alessi DR. 2003. Deficiency of PDK1 in cardiac muscle results in heart failure and increased sensitivity to hypoxia. EMBO J 22(18):4666-76. [PubMed: 12970179] [MGI Ref ID J:85498]
Mora A; Sakamoto K; McManus EJ; Alessi DR. 2005. Role of the PDK1-PKB-GSK3 pathway in regulating glycogen synthase and glucose uptake in the heart. FEBS Lett 579(17):3632-8. [PubMed: 15961082] [MGI Ref ID J:99781]
Mourkioti F; Kratsios P; Luedde T; Song YH; Delafontaine P; Adami R; Parente V; Bottinelli R; Pasparakis M; Rosenthal N. 2006. Targeted ablation of IKK2 improves skeletal muscle strength, maintains mass, and promotes regeneration. J Clin Invest 116(11):2945-54. [PubMed: 17080195] [MGI Ref ID J:114678]
Murray NR; Weems J; Braun U; Leitges M; Fields AP. 2009. Protein kinase C betaII and PKCiota/lambda: collaborating partners in colon cancer promotion and progression. Cancer Res 69(2):656-62. [PubMed: 19147581] [MGI Ref ID J:143707]
Nojiri H; Shimizu T; Funakoshi M; Yamaguchi O; Zhou H; Kawakami S; Ohta Y; Sami M; Tachibana T; Ishikawa H; Kurosawa H; Kahn RC; Otsu K; Shirasawa T. 2006. Oxidative stress causes heart failure with impaired mitochondrial respiration. J Biol Chem 281(44):33789-801. [PubMed: 16959785] [MGI Ref ID J:117386]
Norris AW; Chen L; Fisher SJ; Szanto I; Ristow M; Jozsi AC; Hirshman MF; Rosen ED; Goodyear LJ; Gonzalez FJ; Spiegelman BM; Kahn CR. 2003. Muscle-specific PPARgamma-deficient mice develop increased adiposity and insulin resistance but respond to thiazolidinediones. J Clin Invest 112(4):608-18. [PubMed: 12925701] [MGI Ref ID J:85125]
Norris AW; Hirshman MF; Yao J; Jessen N; Musi N; Chen L; Sivitz WI; Goodyear LJ; Kahn CR. 2008. Endogenous peroxisome proliferator-activated receptor-gamma augments fatty acid uptake in oxidative muscle. Endocrinology 149(11):5374-83. [PubMed: 18653710] [MGI Ref ID J:145477]
Park CB; Asin-Cayuela J; Camara Y; Shi Y; Pellegrini M; Gaspari M; Wibom R; Hultenby K; Erdjument-Bromage H; Tempst P; Falkenberg M; Gustafsson CM; Larsson NG. 2007. MTERF3 is a negative regulator of mammalian mtDNA transcription. Cell 130(2):273-85. [PubMed: 17662942] [MGI Ref ID J:145125]
Pospisilik JA; Knauf C; Joza N; Benit P; Orthofer M; Cani PD; Ebersberger I; Nakashima T; Sarao R; Neely G; Esterbauer H; Kozlov A; Kahn CR; Kroemer G; Rustin P; Burcelin R; Penninger JM. 2007. Targeted deletion of AIF decreases mitochondrial oxidative phosphorylation and protects from obesity and diabetes. Cell 131(3):476-91. [PubMed: 17981116] [MGI Ref ID J:141454]
Potthoff MJ; Arnold MA; McAnally J; Richardson JA; Bassel-Duby R; Olson EN. 2007. Regulation of skeletal muscle sarcomere integrity and postnatal muscle function by Mef2c. Mol Cell Biol 27(23):8143-51. [PubMed: 17875930] [MGI Ref ID J:129041]
Princen F; Bard E; Sheikh F; Zhang SS; Wang J; Zago WM; Wu D; Trelles RD; Bailly-Maitre B; Kahn CR; Chen Y; Reed JC; Tong GG; Mercola M; Chen J; Feng GS. 2009. Deletion of Shp2 tyrosine phosphatase in muscle leads to dilated cardiomyopathy, insulin resistance, and premature death. Mol Cell Biol 29(2):378-88. [PubMed: 19001090] [MGI Ref ID J:144767]
Rohl M; Pasparakis M; Baudler S; Baumgartl J; Gautam D; Huth M; De Lorenzi R; Krone W; Rajewsky K; Bruning JC. 2004. Conditional disruption of IkappaB kinase 2 fails to prevent obesity-induced insulin resistance. J Clin Invest 113(3):474-81. [PubMed: 14755344] [MGI Ref ID J:87587]
Ruffell D; Mourkioti F; Gambardella A; Kirstetter P; Lopez RG; Rosenthal N; Nerlov C. 2009. A CREB-C/EBPbeta cascade induces M2 macrophage-specific gene expression and promotes muscle injury repair. Proc Natl Acad Sci U S A 106(41):17475-80. [PubMed: 19805133] [MGI Ref ID J:153686]
Sajan MP; Standaert ML; Nimal S; Varanasi U; Pastoor T; Mastorides S; Braun U; Leitges M; Farese RV. 2009. The critical role of atypical protein kinase C in activating hepatic SREBP-1c and NFkappaB in obesity. J Lipid Res 50(6):1133-45. [PubMed: 19202134] [MGI Ref ID J:149920]
Sakamoto K; McCarthy A; Smith D; Green KA; Grahame Hardie D; Ashworth A; Alessi DR. 2005. Deficiency of LKB1 in skeletal muscle prevents AMPK activation and glucose uptake during contraction. EMBO J 24(10):1810-20. [PubMed: 15889149] [MGI Ref ID J:98513]
Sakamoto K; Zarrinpashneh E; Budas GR; Pouleur AC; Dutta A; Prescott AR; Vanoverschelde JL; Ashworth A; Jovanovic A; Alessi DR; Bertrand L. 2006. Deficiency of LKB1 in heart prevents ischemia-mediated activation of AMPKalpha2 but not AMPKalpha1. Am J Physiol Endocrinol Metab 290(5):E780-8. [PubMed: 16332922] [MGI Ref ID J:115768]
Shrimali RK; Weaver JA; Miller GF; Starost MF; Carlson BA; Novoselov SV; Kumaraswamy E; Gladyshev VN; Hatfield DL. 2007. Selenoprotein expression is essential in endothelial cell development and cardiac muscle function. Neuromuscul Disord 17(2):135-42. [PubMed: 17142041] [MGI Ref ID J:129594]
Sidhu S; Gangasani A; Korotchkina LG; Suzuki G; Fallavollita JA; Canty JM Jr; Patel MS. 2008. Tissue-specific pyruvate dehydrogenase complex deficiency causes cardiac hypertrophy and sudden death of weaned male mice. Am J Physiol Heart Circ Physiol 295(3):H946-H952. [PubMed: 18586888] [MGI Ref ID J:141289]
Srivastava M; Hsieh S; Grinberg A; Williams-Simons L; Huang SP; Pfeifer K. 2000. H19 and Igf2 monoallelic expression is regulated in two distinct ways by a shared cis acting regulatory region upstream of H19. Genes Dev 14(10):1186-95. [PubMed: 10817754] [MGI Ref ID J:82798]
Tang W; Ingalls CP; Durham WJ; Snider J; Reid MB; Wu G; Matzuk MM; Hamilton SL. 2004. Altered excitation-contraction coupling with skeletal muscle specific FKBP12 deficiency. FASEB J 18(13):1597-9. [PubMed: 15289441] [MGI Ref ID J:94747]
Thomson DM; Brown JD; Fillmore N; Condon BM; Kim HJ; Barrow JR; Winder WW. 2007. LKB1 and the regulation of malonyl-CoA and fatty acid oxidation in muscle. Am J Physiol Endocrinol Metab 293(6):E1572-9. [PubMed: 17925454] [MGI Ref ID J:130093]
Thomson DM; Porter BB; Tall JH; Kim HJ; Barrow JR; Winder WW. 2007. Skeletal muscle and heart LKB1 deficiency causes decreased voluntary running and reduced muscle mitochondrial marker enzyme expression in mice. Am J Physiol Endocrinol Metab 292(1):E196-202. [PubMed: 16926377] [MGI Ref ID J:116973]
Wagner PD; Olfert IM; Tang K; Breen EC. 2006. Muscle-targeted deletion of VEGF and exercise capacity in mice. Respir Physiol Neurobiol 151(2-3):159-66. [PubMed: 16344007] [MGI Ref ID J:128241]
Wang X; Blagden C; Fan J; Nowak SJ; Taniuchi I; Littman DR; Burden SJ. 2005. Runx1 prevents wasting, myofibrillar disorganization, and autophagy of skeletal muscle. Genes Dev 19(14):1715-22. [PubMed: 16024660] [MGI Ref ID J:99622]
Wang Z; Wang B; Yang L; Guo Q; Aithmitti N; Songyang Z; Zheng H. 2009. Presynaptic and postsynaptic interaction of the amyloid precursor protein promotes peripheral and central synaptogenesis. J Neurosci 29(35):10788-801. [PubMed: 19726636] [MGI Ref ID J:152457]
Wijesekara N; Konrad D; Eweida M; Jefferies C; Liadis N; Giacca A; Crackower M; Suzuki A; Mak TW; Kahn CR; Klip A; Woo M. 2005. Muscle-specific Pten deletion protects against insulin resistance and diabetes. Mol Cell Biol 25(3):1135-45. [PubMed: 15657439] [MGI Ref ID J:95993]
Wojtaszewski JF; Higaki Y; Hirshman MF; Michael MD; Dufresne SD; Kahn CR; Goodyear LJ. 1999. Exercise modulates postreceptor insulin signaling and glucose transport in muscle-specific insulin receptor knockout mice. J Clin Invest 104(9):1257-64. [PubMed: 10545524] [MGI Ref ID J:58296]
Xue B; Pulinilkunnil T; Murano I; Bence KK; He H; Minokoshi Y; Asakura K; Lee A; Haj F; Furukawa N; Catalano KJ; Delibegovic M; Balschi JA; Cinti S; Neel BG; Kahn BB. 2009. Neuronal protein tyrosine phosphatase 1B deficiency results in inhibition of hypothalamic AMPK and isoform-specific activation of AMPK in peripheral tissues. Mol Cell Biol 29(16):4563-73. [PubMed: 19528236] [MGI Ref ID J:151519]
Yechoor VK; Patti ME; Ueki K; Laustsen PG; Saccone R; Rauniyar R; Kahn CR. 2004. Distinct pathways of insulin-regulated versus diabetes-regulated gene expression: an in vivo analysis in MIRKO mice. Proc Natl Acad Sci U S A 101(47):16525-30. [PubMed: 15546994] [MGI Ref ID J:123452]
Zabolotny JM; Haj FG; Kim YB; Kim HJ; Shulman GI; Kim JK; Neel BG; Kahn BB. 2004. Transgenic overexpression of protein-tyrosine phosphatase 1B in muscle causes insulin resistance, but overexpression with leukocyte antigen-related phosphatase does not additively impair insulin action. J Biol Chem 279(23):24844-51. [PubMed: 15031294] [MGI Ref ID J:122895]
Zisman A; Peroni OD; Abel ED; Michael MD; Mauvais-Jarvis F; Lowell BB; Wojtaszewski JF; Hirshman MF; Virkamaki A; Goodyear LJ; Kahn CR; Kahn BB. 2000. Targeted disruption of the glucose transporter 4 selectively in muscle causes insulin resistance and glucose intolerance. Nat Med 6(8):924-8. [PubMed: 10932232] [MGI Ref ID J:75418]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice are bred as hemizygotes. While the donating investigator has not attempted to make this strain homozygous, viability of homozygous mice is expected. Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $295.00 Female or Male Hemizygous for Tg(Ckmm-cre)5Khn
Pairs /Price (US dollars $) Pair Genotype $349.35 Hemizygous for Tg(Ckmm-cre)5Khn x Noncarrier $349.35 Noncarrier x Hemizygous for Tg(Ckmm-cre)5Khn
Additional Supply Details
| Pricing for International shipping destinations |
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Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $383.50 Female or Male Hemizygous for Tg(Ckmm-cre)5Khn
Pairs /Price (US dollars $) Pair Genotype $454.20 Hemizygous for Tg(Ckmm-cre)5Khn x Noncarrier $454.20 Noncarrier x Hemizygous for Tg(Ckmm-cre)5Khn
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
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| Supply Notes |
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Control Information
| Control | ||
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| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
Purchasing Information
JAX® Mice Orders
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Orders & Technical Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
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| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.
No Liability
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. In purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.