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| These ZW mice harbor a Cre-conditional pCZW transgene and have widespread expression of lacZ under the control of the CMV enhancer/chicken actin promoter. When bred to mice expressing Cre recombinase, the "floxed" lacZ sequence is removed in the resulting offspring, and lacZ expression is replaced by wheat germ agglutinin (plant lectin) expression in all cre-expressing cells. These ZW mice may be useful for global expression of lacZ or, when crossed to a cre-expressing strain, for tracing transneuronal or trans-synaptic connections and circuits in brain regions or in the spinal cord. | |||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
Description
Strain Information
Former Names B6.Cg-Tg(ACTB-lacZ-WGA)330Bbm/J (Changed: 08-MAY-08 ) Type Congenic; Mutant Strain; Transgenic; Mating System Noncarrier x Hemizygote (Female x Male) Species laboratory mouse Generation ?+N2 (11-JUN-07) Donating Investigator IMR Colony, The Jackson Laboratory Description
These ZW transgenic mice constitutively express lacZ under the control of the CMV enhancer/chicken actin promoter. Expression is widespread, but mosaic, throughout the central and peripheral nervous systems. Purkinje cells display intense beta-galactosidase activity. Approximately 50% of the total neuron population express the transgene, as detected by beta-galactosidase activity. Newborn mice exhibit widespread beta-galactosidase activity. When crossed with a Cre recombinase-expressing strain, lacZ expression is replaced with wheat germ agglutinin (plant lectin) expression in tissues expressing cre. The double reporter system makes it possible to distinguish a lack of reporter (lacZ) expression from a lack of Cre recombinase expression while providing a means to assess Cre excision activity at the individual cell level. This transgenic mouse strain may be useful in tracing transneuronal or trans-synaptic connections and circuits in brain regions or in the spinal cord. Mice homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
lacZ Expression Strains
View lacZ Expression Strains (174 strains)
006465 B6;CBA-Tg(CAG-lacZ-WGA)330Bbm/J
Additional Web Information
Congenic Nomenclature
Cre-lox Systems
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
Mammalian Phenotype Terms assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Tg(CAG-lacZ-WGA)330Bbm/Tg(CAG-lacZ-WGA)330Bbm
involves: C57BL/6 * CBA/J
- normal phenotype
- no abnormal phenotype detected (MGI Ref ID J:92042)
- mice homozygous for the transgene are viable and exhibit no physical or behavioral abnormalities
Research Applications
This mouse can be used to support research in many areas including:
lacZ relatedResearch Tools
lacZ Expression
Cell Biology Research
Cre-lox System (loxP-flanked Sequences: Test/Reporter)
Genetics Research (Tissue/Cell Markers: multiple)
Genetics Research (Tissue/Cell Markers: neurons)
Neurobiology Research (cell marker)
Research Tools
lacZ Expression
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(CAG-lacZ-WGA)330Bbm | ||
|---|---|---|---|
| Allele Name | transgene insertion 330, Allan Basbaum | ||
| Common Name(s) | Tg(ACTB-lacZ-WGA)330Bbm; Tg(CAG-lacZ-WGA)330Bbm; ZW; ZW19; | ||
| Mutation Made By | Joao Braz, University of California San Francisco | ||
| Strain of Origin | (C57BL/6J x CBA/J)F1 | ||
| Site of Expression | lacZ is expressed throughout central and peripheral nervous system; when crossed with a cre recombinase-expressing strain, lacZ expression is replaced with wheat germ agglutinin expression | ||
| Expressed Gene | WGA, Wheat Germ Agglutin, truncated, | ||
| Expressed Gene | lacZ, beta-galactosidase, E. coli | ||
| Promoter | ACTB, actin, beta, chicken | ||
| General Note | Of three transgenic lines generated - called ZW5, ZW16 and ZW19 by Braz and colleagues (2002) - only ZW19, which exhibited the highest transmission rates, was propagated. | ||
| Molecular Note | The transgene comprises a minimal immediate-early CMV enhancer/chicken beta-actin promoter driving ubiquitous expression of a lox-P flanked beta-galactosidase/neomycin phosphotransferase II (beta-geo) fusion gene that terminates in a strong, triple polyadenylation signal, followed by a C-terminally truncated wheat germ agglutinin coding sequence. Neuron-specific excision of the beta-geo gene by Cre recombinase permits expression of the lectin, whose migration allows tracing of synapses involving the expressing cells. [MGI Ref ID J:92042] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic LacZ Melt Curve Analysis, MCA, vers. 2
Generic LacZ, STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Braz JM; Rico B; Basbaum AI. 2002. Transneuronal tracing of diverse CNS circuits by Cre-mediated induction of wheat germ agglutinin in transgenic mice. Proc Natl Acad Sci U S A 99(23):15148-53. [PubMed: 12391304] [MGI Ref ID J:92042]
Additional References
Tg(CAG-lacZ-WGA)330Bbm related
Braz JM; Nassar MA; Wood JN; Basbaum AI. 2005. Parallel 'pain' pathways arise from subpopulations of primary afferent nociceptor. Neuron 47(6):787-93. [PubMed: 16157274] [MGI Ref ID J:100993]Kim IJ; Zhang Y; Yamagata M; Meister M; Sanes JR. 2008. Molecular identification of a retinal cell type that responds to upward motion. Nature 452(7186):478-82. [PubMed: 18368118] [MGI Ref ID J:134167]
Health & husbandry
Health & Colony Maintenance Information
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, hemizygous mice are bred to C57BL/6J inbred mice. Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
This strain is currently Under Development for Distribution Colony.
To register your interest in this strain go to the Strain Interest Form.
Estimated Available for Sale Date:
Please note: Estimated available for sale dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for sale depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain/interest registered.
View All Strains Under Development
Pricing
Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $236.40 Female or Male Hemizygous for Tg(CAG-lacZ-WGA)330Bbm *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $288.65 Hemizygous for Tg(CAG-lacZ-WGA)330Bbm x Noncarrier $288.65 Noncarrier x Hemizygous for Tg(CAG-lacZ-WGA)330Bbm
Supply Details
| Standard Supply | Under Development for Distribution Colony |
|---|---|
| Supply Notes |
|
Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $307.40 Female or Male Hemizygous for Tg(CAG-lacZ-WGA)330Bbm *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $375.30 Hemizygous for Tg(CAG-lacZ-WGA)330Bbm x Noncarrier $375.30 Noncarrier x Hemizygous for Tg(CAG-lacZ-WGA)330Bbm
Supply Details
| Standard Supply | Under Development for Distribution Colony |
|---|---|
| Supply Notes |
|
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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