Strain Name:

C57BL/6J-Tg(ACTB-NOTCH1)1Shn/J

Stock Number:

006481

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Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Coisogenic; Mutant Strain; Transgenic;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
Generation?+N1F4pN1
Generation Definitions
 
Donating Investigator Jie Shen,   Harvard Med Sch/Brigham Women's Hosp

Description
Transgenic mice are viable, fertile and behaviorally normal. These "CALSL-NICD (H)" mice (or simply CALSL-NICD) reportedly carry 10-20 copies of the transgene inserted into a single genomic locus. Expression of the transgene-derived intracellular domain of human NOTCH1 is prevented by a "Lox-STOP-Lox" cassette. When transgenic mice are bred to a strain expressing Cre recombinase, the "floxed stop" cassette is excised in the resulting offspring, and human NOTCH1 expression is observed in the cre-expressing tissue(s). These transgenic mice may be useful in studying early neural progenitor cell development and apoptosis, and responses to tissue-specific Notch activation.

For example, when crossed to a strain expressing Cre recombinase in the nervous system (see Stock No. 003771), this transgenic mouse strain may be useful in studies of notch signaling during apoptotic cell death.

Development
The human Notch1 intracellular domain (NICD) (amino acid residues 1762–2304; including the RAM23 domain, CDC10/Ankyrin repeats, and two nuclear localization signals), as well as a SV40 late polyadenylation signal was inserted downstream of a chicken beta-actin-loxP-stop-loxP sequence. The transgene was injected into the pronucleus of C57BL/6J embryos. The resulting transgenic mice with 10-20 copies of the transgene at a single insertion site (called CALSL-NICD (H) or simply CALSL-NICD), were subsequently bred to C57BL/6J mice for many generations prior to arrival at The Jackson Laboratory.

Control Information

  Control
   Noncarrier
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of ACTB
005483   129-Tg(CAG-EYFP)7AC5Nagy/J
004178   B6.129(Cg)-Tg(CAG-Bgeo/GFP)21Lbe/J
018979   B6.129(Cg)-Tg(CAG-EGFP)D4Nagy/KnwJ
004218   B6.129(ICR)-Tg(CAG-ECFP)CK6Nagy/J
021930   B6.129S1-Tg(CAG-EGFP)S1C2Tpo/KnwPeaJ
021146   B6.Cg-Fcgrttm1Dcr Prkdcscid Tg(CAG-FCGRT)276Dcr/DcrJ
004919   B6.Cg-Fcgrttm1Dcr Tg(CAG-FCGRT)276Dcr/DcrJ
016919   B6.Cg-Rag1tm1Mom Fcgrttm1Dcr Tg(CAG-FCGRT)276Dcr/DcrJ
006051   B6.Cg-Tg(CAG-DsRed*MST)1Nagy/J
008705   B6.Cg-Tg(CAG-DsRed,-EGFP)5Gae/J
007575   B6.Cg-Tg(CAG-Ngb,-EGFP)1Dgrn/J
007241   B6.Cg-Tg(CAG-Serpine1)1Dgi/J
008111   B6.Cg-Tg(CAG-Ub*G76V/GFP)1Dant/J
008112   B6.Cg-Tg(CAG-Ub*G76V/GFP)2Dant/J
004682   B6.Cg-Tg(CAG-cre/Esr1*)5Amc/J
005884   B6.Cg-Tg(CAG-mRFP1)1F1Hadj/J
006069   B6.Cg-Tg(HIST1H2BB/EGFP)1Pa/J
002982   B6.Cg-Tg(xstpx-lacZ)32And/J
012436   B6;129S7-Tg(CAG-lacZ,-BMPR1A*,-EGFP)1Mis/Mmjax
008605   B6;C3-Tg(CAG-DsRed,-EGFP)5Gae/J
008080   B6;C3-Tg(CAG-SAC/EGFP)35Rang/J
006465   B6;CBA-Tg(CAG-lacZ-WGA)330Bbm/J
010967   B6;D2-Tg(Actb-Uprt)274Cdoe/J
009668   B6;D2-Tg(Actb-Uprt)372Cdoe/J
019102   B6N.Cg-Tg(CAG-cre/Esr1*)5Amc/CjDswJ
010545   C.FVB-Tg(CAG-luc,-GFP)L2G85Chco/FathJ
006662   C57BL/6-Tg(ACTB-MAP2K1*K97M)1Stl/J
008234   CB6-Tg(CAG-EGFP/CETN2)3-4Jgg/J
010548   D1.FVB(Cg)-Tg(CAG-luc,-GFP)L2G85Chco/FathJ
017881   FVB-Tg(CAG-Diap3)924Lesp/J
008450   FVB-Tg(CAG-luc,-GFP)L2G85Chco/J
003516   FVB.Cg-Tg(CAG-EGFP)B5Nagy/J
017595   FVB.Cg-Tg(CAG-cre/Esr1*)5Amc/J
023974   NOD.129(Cg)-Tg(CAG-Bgeo/ALPP)1Lbe/SbwJ
017620   NOD.Cg-Prkdcscid Tg(CAG-DsRed*MST)1Nagy/KupwJ
010542   NOD.FVB-Tg(CAG-luc,-GFP)L2G85Chco/FathJ
005082   NOD/ShiLt-Tg(ACTB-Ica1/EGFP)18Mdos/MdosJ
017932   STOCK Iis3tm1.1(ACTB-EGFP*)Luo/J
017923   STOCK Iis3tm2.1(ACTB-EGFP*,-tdTomato)Luo/J
008813   STOCK Trpa1tm2Kykw Tg(CAG-cre/Esr1*)5Amc/J
014092   STOCK Tg(ACTB-tTA2,-MAPT/lacZ)1Luo/J
006882   STOCK Tg(CAG-Bgeo,-AML1/ETO,-ALPP)1Lbe/J
006850   STOCK Tg(CAG-Bgeo,-NOTCH1,-EGFP)1Lbe/J
006876   STOCK Tg(CAG-Bgeo,-TEL/AML1,-EGFP)A6Lbe/J
006613   STOCK Tg(CAG-Bgeo,-Tle1,-ALPP)1Lbe/J
003919   STOCK Tg(CAG-Bgeo/ALPP)1Lbe/J
003920   STOCK Tg(CAG-Bgeo/GFP)21Lbe/J
005441   STOCK Tg(CAG-DsRed*MST)1Nagy/J
003773   STOCK Tg(CAG-ECFP)CK6Nagy/J
003115   STOCK Tg(CAG-EGFP)B5Nagy/J
003116   STOCK Tg(CAG-EGFP)D4Nagy/J
011106   STOCK Tg(CAG-GFP*)1Hadj/J
009678   STOCK Tg(CAG-RAB9A)500Repa/J
011107   STOCK Tg(CAG-Venus)1Hadj/J
008783   STOCK Tg(CAG-cre/Esr1*)5Amc Smn1tm3(SMN2/Smn1)Mrph Tg(SMN2*delta7)4299Ahmb Tg(SMN2)89Ahmb/J
004453   STOCK Tg(CAG-cre/Esr1*)5Amc/J
005645   STOCK Tg(CAG-mRFP1)1F1Hadj/J
View Strains carrying other alleles of ACTB     (57 strains)

Strains carrying other alleles of NOTCH1
006850   STOCK Tg(CAG-Bgeo,-NOTCH1,-EGFP)1Lbe/J
View Strains carrying other alleles of NOTCH1     (1 strain)

Additional Web Information

Introduction to Cre-lox technology

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on transgenic expression of an ortholog of a human gene that is associated with this disease. Phenotypic similarity to the human disease has not been tested.
Aortic Valve Disease 1; AOVD1   (NOTCH1)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.

Tg(ACTB-NOTCH1)1Shn/0 Tg(Nes-cre)1Kln/0

        B6.Cg-Tg(Nes-cre)1Kln Tg(ACTB-NOTCH1)1Shn   (conditional)
  • mortality/aging
  • complete perinatal lethality   (MGI Ref ID J:90392)
  • partial prenatal lethality
    • some of the mutants die before birth; however, most survive to the perinatal stage   (MGI Ref ID J:90392)
  • nervous system phenotype
  • abnormal cortical ventricular zone morphology
    • at E12 - 13.5 the ventricular zone harboring neural progenitors is thinner   (MGI Ref ID J:90392)
  • abnormal neuron apoptosis
    • at E10 the number of apoptotic cells in the forebrain-midbrain junction is increased   (MGI Ref ID J:90392)
    • by E11.5 apoptosis is more widespread in the telencephalon with about 30% of all cells being TUNEL positive   (MGI Ref ID J:90392)
  • decreased brain size
    • at E12 - 13.5 all brain subregions are smaller compared to wild-type or single transgenic littermates   (MGI Ref ID J:90392)
    • at P0 the brain especially the cerebral cortex is much smaller in double transgenic mice compared to wild-type or single transgenic littermates   (MGI Ref ID J:90392)
  • decreased neuron number
    • the number of postmitotic neurons is decreased at E12 - 13.5 in the telencephalon and diencephalon   (MGI Ref ID J:90392)
  • small embryonic telencephalon
    • at E12 - 13.5 the telencephalon is smaller compared to wild-type or single transgenic littermates   (MGI Ref ID J:90392)
  • cellular phenotype
  • abnormal neuron apoptosis
    • at E10 the number of apoptotic cells in the forebrain-midbrain junction is increased   (MGI Ref ID J:90392)
    • by E11.5 apoptosis is more widespread in the telencephalon with about 30% of all cells being TUNEL positive   (MGI Ref ID J:90392)

Tg(ACTB-NOTCH1)1Shn/0 Tg(Nes-cre)1Kln/0 Trp53tm1Tyj/Trp53+

        involves: 129S2/SvPas * C57BL/6J   (conditional)
  • cellular phenotype
  • abnormal apoptosis
    • at E10 - 11.5 the number of apoptotic cells in the brain is increased   (MGI Ref ID J:90392)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Apoptosis Research
Extracellular Modulators

Cell Biology Research
Signal Transduction

Neurobiology Research
Cre-lox System
      loxP-flanked Sequences
Neurodevelopmental Defects

Research Tools
Apoptosis Research
Cre-lox System
      loxP-flanked Sequences
Developmental Biology Research
      Cre-lox System

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Tg(ACTB-NOTCH1)1Shn
Allele Name transgene insertion 1, Jie Shen
Allele Type Transgenic (Inserted expressed sequence)
Common Name(s) CALSL-NICD;
Mutation Made By Jie Shen,   Harvard Med Sch/Brigham Women's Hosp
Strain of OriginC57BL/6J
Expressed Gene NOTCH1, notch 1, human
Promoter ACTB, actin, beta, chicken
Molecular Note This transgene consists of the chicken beta-actin promoter followed by a loxP flanked stop, the human NOTCH1 intracellular domain (amino acids 1762-2304), and an SV40 late polyadenylation signal. Cre mediated recombination removes the stop and allows expression of the NOTCH1 gene. Only single site insertions were used to generate mice. This line carries 10 - 20 copies of the transgene and protein is detectable by western blot analysis after cre mediated recombination. [MGI Ref ID J:90392]
 
 

Genotyping

Genotyping Information

Genotyping Protocols

Tg(ACTB-Notch1)1Shn, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Yang X; Klein R; Tian X; Cheng HT; Kopan R; Shen J. 2004. Notch activation induces apoptosis in neural progenitor cells through a p53-dependent pathway. Dev Biol 269(1):81-94. [PubMed: 15081359]  [MGI Ref ID J:90392]

Additional References

Tg(ACTB-NOTCH1)1Shn related

Breunig JJ; Silbereis J; Vaccarino FM; Sestan N; Rakic P. 2007. Notch regulates cell fate and dendrite morphology of newborn neurons in the postnatal dentate gyrus. Proc Natl Acad Sci U S A 104(51):20558-63. [PubMed: 18077357]  [MGI Ref ID J:130582]

Kuo CT; Mirzadeh Z; Soriano-Navarro M; Rasin M; Wang D; Shen J; Sestan N; Garcia-Verdugo J; Alvarez-Buylla A; Jan LY; Jan YN. 2006. Postnatal deletion of Numb/Numblike reveals repair and remodeling capacity in the subventricular neurogenic niche. Cell 127(6):1253-64. [PubMed: 17174898]  [MGI Ref ID J:127227]

Natarajan S; Li Y; Miller EE; Shih DJ; Taylor MD; Stearns TM; Bronson RT; Ackerman SL; Yoon JK; Yun K. 2013. Notch1-induced brain tumor models the sonic hedgehog subgroup of human medulloblastoma. Cancer Res 73(17):5381-90. [PubMed: 23852537]  [MGI Ref ID J:201833]

Surzenko N; Crowl T; Bachleda A; Langer L; Pevny L. 2013. SOX2 maintains the quiescent progenitor cell state of postnatal retinal Muller glia. Development 140(7):1445-56. [PubMed: 23462474]  [MGI Ref ID J:194918]

Woodhoo A; Alonso MB; Droggiti A; Turmaine M; D'Antonio M; Parkinson DB; Wilton DK; Al-Shawi R; Simons P; Shen J; Guillemot F; Radtke F; Meijer D; Feltri ML; Wrabetz L; Mirsky R; Jessen KR. 2009. Notch controls embryonic Schwann cell differentiation, postnatal myelination and adult plasticity. Nat Neurosci 12(7):839-47. [PubMed: 19525946]  [MGI Ref ID J:152569]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, hemizygotes are bred to wildtype siblings or to C57BL/6J inbred mice.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2140.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2782.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   Noncarrier
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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