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Strain Name:

C57BL/6J-Tg(ACTB-NOTCH1)1Shn/J

Stock Number:

006481

Availability:

Repository- Live


General Terms and Conditions

Genes & Alleles   ACTB;   NOTCH1;   Tg(ACTB-NOTCH1)1Shn;


Product Information

Strain Details

Type JAX® GEMM® Strain - Coisogenic
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Mutant Strain
Type JAX® GEMM® Strain - Transgenic
Mating System+/+ sibling x Hemizygote         (Female x Male)
Specieslaboratory mouse
Donating Investigator Jie Shen,   Harvard Med Sch/Brigham Women's Hosp
Generation?+N1F3 (30-APR-08)

Strain Description
Transgenic mice are viable, fertile and behaviorally normal. These "CALSL-NICD (H)" mice (or simply CALSL-NICD) reportedly carry 10-20 copies of the transgene inserted into a single genomic locus. Expression of the transgene-derived intracellular domain of human NOTCH1 is prevented by a "Lox-STOP-Lox" cassette. When transgenic mice are bred to a strain expressing Cre recombinase, the "floxed stop" cassette is excised in the resulting offspring, and human NOTCH1 expression is observed in the cre-expressing tissue(s). These transgenic mice may be useful in studying early neural progenitor cell development and apoptosis, and responses to tissue-specific Notch activation.

For example, when crossed to a strain expressing Cre recombinase in the nervous system (see Stock No. 003771), this transgenic mouse strain may be useful in studies of notch signaling during apoptotic cell death.

Strain Development
The human Notch1 intracellular domain (NICD) (amino acid residues 1762–2304; including the RAM23 domain, CDC10/Ankyrin repeats, and two nuclear localization signals), as well as a SV40 late polyadenylation signal was inserted downstream of a chicken beta-actin-loxP-stop-loxP sequence. The transgene was injected into the pronucleus of C57BL/6J embryos. The resulting transgenic mice with 10-20 copies of the transgene at a single insertion site (called CALSL-NICD (H) or simply CALSL-NICD), were subsequently bred to C57BL/6J mice for many generations prior to arrival at The Jackson Laboratory.

Mammalian Phenotype Terms assigned by genotype

The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.

Tg(ACTB-NOTCH1)1Shn/0 Tg(Nes-cre)1Kln/0

        B6.Cg-Tg(ACTB-NOTCH1)1Shn Tg(Nes-cre)1Kln   (conditional)
  • lethality-prenatal/perinatal
  • prenatal lethality (MGI Ref ID J:90392)
    • some of the mutants die before birth however most survive to the perinatal stage
  • cellular phenotype
  • abnormal apoptosis (MGI Ref ID J:90392)
    • at E10 the number of apoptotic cells in the forebrain-midbrain junction is increased
    • by E11.5 apoptosis is more widespread in the telencephalon with about 30% of all cells being TUNEL positive
  • nervous system phenotype
  • abnormal brain ventricle morphology (MGI Ref ID J:90392)
    • at E12 - 13.5 the ventricular zone harboring neural progenitors is thinner
  • abnormal neuron morphology (MGI Ref ID J:90392)
    • the number of postmitotic neurons is decreased at E12 - 13.5 in the telencephalon and diencephalon
  • abnormal telencephalon morphology (MGI Ref ID J:90392)
    • at E12 - 13.5 the telencephalon is smaller compared to wild-type or single transgenic littermates
  • decreased brain size (MGI Ref ID J:90392)
    • at E12 - 13.5 all brain subregions are smaller compared to wild-type or single transgenic littermates
    • at P0 the brain especially the cerebral cortex is much smaller in double transgenic mice compared to wild-type or single transgenic littermates

Tg(ACTB-NOTCH1)1Shn/0 Tg(Nes-cre)1Kln/0 Trp53tm1Tyj/Trp53+

        involves: C57BL/6J   (conditional)
  • cellular phenotype
  • abnormal apoptosis (MGI Ref ID J:90392)
    • at E10 - 11.5 the number of apoptotic cells in the brain is increased

Gene & Allele Details

Allele Symbol Tg(ACTB-NOTCH1)1Shn
Allele Name transgene insertion 1, Jie Shen
Common Name(s) CALSL-NICD;
Mutation Made By Jie Shen,   Harvard Med Sch/Brigham Women's Hosp
Strain of OriginC57BL/6J
Expressed Gene NOTCH1, Notch homolog 1, translocation-associated (Drosophila), human
Promoter ACTB, actin, beta, chicken
Molecular Note This transgene consists of the chicken beta-actin promoter followed by a loxP flanked stop, the human NOTCH1 intracellular domain (amino acids 1762-2304), and an SV40 late polyadenylation signal. Cre mediated recombination removes the stop and allows expression of the NOTCH1 gene. Only single site insertions were used to generate mice. This line carries 10 - 20 copies of the transgene and protein is detectable by western blot analysis after cre mediated recombination. [MGI Ref ID J:90392]

Control Information

  Control
   Noncarrier
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Genotyping Protocols

Tg(ACTB-Notch1)1Shn

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, hemizygotes are bred to wildtype siblings or to C57BL/6J inbred mice.
Diet Information LabDiet® 5K52/5K67

Related Strains

View Strains carrying other alleles of ACTB     (31 strains)

Strains carrying other alleles of NOTCH1
006850   STOCK Tg(CAG-Bgeo,-NOTCH1,-EGFP)1Lbe/J
View Strains carrying other alleles of NOTCH1     (1 strain)

Additional Web Information

Cre-lox or FLP-FRT Systems

Animal Health Reports

Room Number           AX11

Research Applications

This mouse can be used to support research in many areas including:

Apoptosis Research
Extracellular Modulators

Cell Biology Research
Signal Transduction

Neurobiology Research
Cre-lox System (loxP-flanked Sequences)
Neurodevelopmental Defects

Research Tools
Apoptosis Research
Cre-lox System (loxP-flanked Sequences)
Developmental Biology Research (Cre-lox System)

References

Selected Reference(s)

Yang X; Klein R; Tian X; Cheng HT; Kopan R; Shen J. 2004. Notch activation induces apoptosis in neural progenitor cells through a p53-dependent pathway. Dev Biol 269(1):81-94. [PubMed: 15081359]  [MGI Ref ID J:90392]

Additional References

Price and Supply Information

Strain Name: C57BL/6J-Tg(ACTB-NOTCH1)1Shn/J
Stock Number: 006481

Price Details

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Supply Details

Standard SupplyRepository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes Usually shipped between four and eight weeks of age.
This strain is included in the Induced Mutant Resource Colony collection.
LicensingSee General Terms and Conditions below for Licensing and Use Restrictions  
Control InformationView Control Information in Strain Details.

General Terms and Conditions

View JAX® Mice & Services Conditions of Use.

Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.

For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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