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- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Coisogenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation ?+N1F4pN1
Generation DefinitionsDonating Investigator Jie Shen, Harvard Med Sch/Brigham Women's Hosp Description
Transgenic mice are viable, fertile and behaviorally normal. These "CALSL-NICD (H)" mice (or simply CALSL-NICD) reportedly carry 10-20 copies of the transgene inserted into a single genomic locus. Expression of the transgene-derived intracellular domain of human NOTCH1 is prevented by a "Lox-STOP-Lox" cassette. When transgenic mice are bred to a strain expressing Cre recombinase, the "floxed stop" cassette is excised in the resulting offspring, and human NOTCH1 expression is observed in the cre-expressing tissue(s). These transgenic mice may be useful in studying early neural progenitor cell development and apoptosis, and responses to tissue-specific Notch activation.For example, when crossed to a strain expressing Cre recombinase in the nervous system (see Stock No. 003771), this transgenic mouse strain may be useful in studies of notch signaling during apoptotic cell death.
Development
The human Notch1 intracellular domain (NICD) (amino acid residues 1762–2304; including the RAM23 domain, CDC10/Ankyrin repeats, and two nuclear localization signals), as well as a SV40 late polyadenylation signal was inserted downstream of a chicken beta-actin-loxP-stop-loxP sequence. The transgene was injected into the pronucleus of C57BL/6J embryos. The resulting transgenic mice with 10-20 copies of the transgene at a single insertion site (called CALSL-NICD (H) or simply CALSL-NICD), were subsequently bred to C57BL/6J mice for many generations prior to arrival at The Jackson Laboratory.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of ACTB
View Strains carrying other alleles of ACTB (56 strains)
006850 STOCK Tg(CAG-Bgeo,-NOTCH1,-EGFP)1Lbe/J
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
- Potential model based on transgenic expression of an ortholog of a human gene that is associated with this disease. Phenotypic similarity to the human disease has not been tested. Aortic Valve Disease 1; AOVD1 (NOTCH1)
assigned by genotype
The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.Tg(ACTB-NOTCH1)1Shn/0 Tg(Nes-cre)1Kln/0
B6.Cg-Tg(Nes-cre)1Kln Tg(ACTB-NOTCH1)1Shn (conditional)
- mortality/aging
- complete perinatal lethality (MGI Ref ID J:90392)
- partial prenatal lethality
- some of the mutants die before birth; however, most survive to the perinatal stage (MGI Ref ID J:90392)
- nervous system phenotype
- abnormal neuron apoptosis
- abnormal telencephalon morphology
- at E12 - 13.5 the telencephalon is smaller compared to wild-type or single transgenic littermates (MGI Ref ID J:90392)
- abnormal cortical ventricular zone morphology
- at E12 - 13.5 the ventricular zone harboring neural progenitors is thinner (MGI Ref ID J:90392)
- decreased brain size
- decreased neuron number
- the number of postmitotic neurons is decreased at E12 - 13.5 in the telencephalon and diencephalon (MGI Ref ID J:90392)
- cellular phenotype
- abnormal neuron apoptosis
Tg(ACTB-NOTCH1)1Shn/0 Tg(Nes-cre)1Kln/0 Trp53tm1Tyj/Trp53+
involves: 129S2/SvPas * C57BL/6J (conditional)
- cellular phenotype
- abnormal apoptosis
- at E10 - 11.5 the number of apoptotic cells in the brain is increased (MGI Ref ID J:90392)
This mouse can be used to support research in many areas including:
Apoptosis Research
Extracellular Modulators
Cell Biology Research
Signal Transduction
Neurobiology Research
Cre-lox System
loxP-flanked Sequences
Neurodevelopmental Defects
Research Tools
Apoptosis Research
Cre-lox System
loxP-flanked Sequences
Developmental Biology Research
Cre-lox System
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(ACTB-NOTCH1)1Shn | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Jie Shen | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | CALSL-NICD; | ||
| Mutation Made By | Jie Shen, Harvard Med Sch/Brigham Women's Hosp | ||
| Strain of Origin | C57BL/6J | ||
| Expressed Gene | NOTCH1, notch 1, human | ||
| Promoter | ACTB, actin, beta, chicken | ||
| Molecular Note | This transgene consists of the chicken beta-actin promoter followed by a loxP flanked stop, the human NOTCH1 intracellular domain (amino acids 1762-2304), and an SV40 late polyadenylation signal. Cre mediated recombination removes the stop and allows expression of the NOTCH1 gene. Only single site insertions were used to generate mice. This line carries 10 - 20 copies of the transgene and protein is detectable by western blot analysis after cre mediated recombination. [MGI Ref ID J:90392] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(ACTB-Notch1)1Shn, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Yang X; Klein R; Tian X; Cheng HT; Kopan R; Shen J. 2004. Notch activation induces apoptosis in neural progenitor cells through a p53-dependent pathway. Dev Biol 269(1):81-94. [PubMed: 15081359] [MGI Ref ID J:90392]
Additional References
Tg(ACTB-NOTCH1)1Shn relatedBreunig JJ; Silbereis J; Vaccarino FM; Sestan N; Rakic P. 2007. Notch regulates cell fate and dendrite morphology of newborn neurons in the postnatal dentate gyrus. Proc Natl Acad Sci U S A 104(51):20558-63. [PubMed: 18077357] [MGI Ref ID J:130582]
Kuo CT; Mirzadeh Z; Soriano-Navarro M; Rasin M; Wang D; Shen J; Sestan N; Garcia-Verdugo J; Alvarez-Buylla A; Jan LY; Jan YN. 2006. Postnatal deletion of Numb/Numblike reveals repair and remodeling capacity in the subventricular neurogenic niche. Cell 127(6):1253-64. [PubMed: 17174898] [MGI Ref ID J:127227]
Surzenko N; Crowl T; Bachleda A; Langer L; Pevny L. 2013. SOX2 maintains the quiescent progenitor cell state of postnatal retinal Muller glia. Development 140(7):1445-56. [PubMed: 23462474] [MGI Ref ID J:194918]
Woodhoo A; Alonso MB; Droggiti A; Turmaine M; D'Antonio M; Parkinson DB; Wilton DK; Al-Shawi R; Simons P; Shen J; Guillemot F; Radtke F; Meijer D; Feltri ML; Wrabetz L; Mirsky R; Jessen KR. 2009. Notch controls embryonic Schwann cell differentiation, postnatal myelination and adult plasticity. Nat Neurosci 12(7):839-47. [PubMed: 19525946] [MGI Ref ID J:152569]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry When maintaining a live colony, hemizygotes are bred to wildtype siblings or to C57BL/6J inbred mice.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1980.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2574.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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Terms of Use
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General Terms and Conditions
For Licensing and Use Restrictions view the link(s) below:
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Contact information
General inquiries regarding Terms of UseContracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
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