Strain Name: |
C57BL/6J-Tg(ACTB-NOTCH1)1Shn/J |
|---|---|
Stock Number: |
006481 |
Availability: | Repository- Live |
General Terms and Conditions |
| Genes & Alleles | ACTB; NOTCH1; Tg(ACTB-NOTCH1)1Shn; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Coisogenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Mating System +/+ sibling x Hemizygote (Female x Male) Species laboratory mouse Donating Investigator Jie Shen, Harvard Med Sch/Brigham Women's Hosp Generation ?+N1F3 (30-APR-08) Strain Description
Transgenic mice are viable, fertile and behaviorally normal. These "CALSL-NICD (H)" mice (or simply CALSL-NICD) reportedly carry 10-20 copies of the transgene inserted into a single genomic locus. Expression of the transgene-derived intracellular domain of human NOTCH1 is prevented by a "Lox-STOP-Lox" cassette. When transgenic mice are bred to a strain expressing Cre recombinase, the "floxed stop" cassette is excised in the resulting offspring, and human NOTCH1 expression is observed in the cre-expressing tissue(s). These transgenic mice may be useful in studying early neural progenitor cell development and apoptosis, and responses to tissue-specific Notch activation.For example, when crossed to a strain expressing Cre recombinase in the nervous system (see Stock No. 003771), this transgenic mouse strain may be useful in studies of notch signaling during apoptotic cell death.
Strain Development
The human Notch1 intracellular domain (NICD) (amino acid residues 1762–2304; including the RAM23 domain, CDC10/Ankyrin repeats, and two nuclear localization signals), as well as a SV40 late polyadenylation signal was inserted downstream of a chicken beta-actin-loxP-stop-loxP sequence. The transgene was injected into the pronucleus of C57BL/6J embryos. The resulting transgenic mice with 10-20 copies of the transgene at a single insertion site (called CALSL-NICD (H) or simply CALSL-NICD), were subsequently bred to C57BL/6J mice for many generations prior to arrival at The Jackson Laboratory.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Tg(ACTB-NOTCH1)1Shn | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Jie Shen | ||
| Common Name(s) | CALSL-NICD; | ||
| Mutation Made By | Jie Shen, Harvard Med Sch/Brigham Women's Hosp | ||
| Strain of Origin | C57BL/6J | ||
| Expressed Gene | NOTCH1, Notch homolog 1, translocation-associated (Drosophila), human | ||
| Promoter | ACTB, actin, beta, chicken | ||
| Molecular Note | This transgene consists of the chicken beta-actin promoter followed by a loxP flanked stop, the human NOTCH1 intracellular domain (amino acids 1762-2304), and an SV40 late polyadenylation signal. Cre mediated recombination removes the stop and allows expression of the NOTCH1 gene. Only single site insertions were used to generate mice. This line carries 10 - 20 copies of the transgene and protein is detectable by western blot analysis after cre mediated recombination. [MGI Ref ID J:90392] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Tg(ACTB-Notch1)1Shn
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, hemizygotes are bred to wildtype siblings or to C57BL/6J inbred mice. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying other alleles of ACTB
View Strains carrying other alleles of ACTB (31 strains)
006850 STOCK Tg(CAG-Bgeo,-NOTCH1,-EGFP)1Lbe/J
Additional Web Information
Cre-lox or FLP-FRT Systems
Animal Health Reports
Room Number AX11
Research Applications
This mouse can be used to support research in many areas including:
Apoptosis Research
Extracellular Modulators
Cell Biology Research
Signal Transduction
Neurobiology Research
Cre-lox System (loxP-flanked Sequences)
Neurodevelopmental Defects
Research Tools
Apoptosis Research
Cre-lox System (loxP-flanked Sequences)
Developmental Biology Research (Cre-lox System)
References
Selected Reference(s)
Additional ReferencesYang X; Klein R; Tian X; Cheng HT; Kopan R; Shen J. 2004. Notch activation induces apoptosis in neural progenitor cells through a p53-dependent pathway. Dev Biol 269(1):81-94. [PubMed: 15081359] [MGI Ref ID J:90392]
Price and Supply Information
| Strain Name: | C57BL/6J-Tg(ACTB-NOTCH1)1Shn/J |
| Stock Number: | 006481 |
Price Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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