Strain Name: |
B6.129S4-Abcb7tm1Mdf/J |
|---|---|
Stock Number: |
006490 |
Availability: | Repository- Live |
General Terms and Conditions |
| Genes & Alleles | Abcb7; Abcb7tm1Mdf; |
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Mating System Homozygote x Hemizygote (Female x Male) Species laboratory mouse Donating Investigator Mark Fleming, Children's Hospital Boston Generation N10F?+2pN1F3 (06-AUG-08) Strain Description
Homozygous mice are viable and fertile with no reported neurological or hematological abnormalities. These mutant mice have loxP sites flanking exons 9 and 10 of the endogenous gene. When bred to Cre recombinase expressing mice, exons 9 and 10 are deleted in the offspring dependent on the tissue specificity of the Cre recombinase expressing parent. The donating investigator reports that the null allele is not transmissible due to an effect on the extraembryonic tissues. This mutant may be useful in studying cytosolic Fe-S cluster assembly and metabolism, Friedreich ataxia, anemia, and hematopoiesis.When bred to a strain expressing Cre recombinase in liver (see Stock No. 003574 for example), this mutant mouse strain may be useful in studies of hepatocyte iron metabolism.
When bred to a strain expressing Cre recombinase in epiblast derived cells (see Stock No. 004783, 008454 for example), this mutant mouse strain may be useful in development studies.
Strain Development
A targeting vector was designed to insert a loxP site into intron 8 and a loxP-flanked neomycin resistance cassette into intron 10 of the X-linked endogenous gene. The construct was electroporated into the 129S4/SvJae-derived J1 embryonic stem (ES) cells which were transiently transfected with a Cre recombinase vector to remove the selection cassette. Correctly targeted ES cells (with loxP sites flanking exons 9 and 10) were injected into blastocysts and the resulting chimeric mice were bred to C57BL/6J to establish the colony. Mutant mice were backcrossed to C57BL/6J mice for 10 generations prior to arrival at The Jackson Laboratory.
Mammalian Phenotype Terms assigned by genotype |
| Allele Symbol | Abcb7tm1Mdf | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Mark D Fleming | ||
| Mutation Made By | Mark Fleming, Children's Hospital Boston | ||
| Strain of Origin | 129S4/SvJae | ||
| ES Cell Line Name | J1 | ||
| ES Cell Line Strain | 129S4/SvJae | ||
| Gene Symbol and Name | Abcb7, ATP-binding cassette, sub-family B (MDR/TAP), member 7 | ||
| Chromosome | X | ||
| Gene Common Name(s) | AA517758; ABC7; ASAT; ATP-binding cassette 7; AU019072; Abc7; Atm1p; EST140535; expressed sequence AA517758; expressed sequence AU019072; | ||
| Molecular Note | LoxP sites were inserted into the locus to flank exons 9 and 10. Transient cre expression removed a neomycin resistance cassette included in the vector. [MGI Ref ID J:106838] | ||
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Abcb7tm1Mdf
| Breeding & Husbandry | The targeted gene is on the X chromosome. When maintaining a live colony, homozygous females are bred to hemizygous males. |
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| Diet Information | LabDiet® 5K52/5K67 |
Congenic Nomenclature
Cre-lox or FLP-FRT Systems
Room Number AX11
Hematological Research
Anemia, Iron Deficiency and Transport Defects
Anemia, Iron Homeostasis Defects
Neurobiology Research
Ataxia (Movement) Defects
Research Tools
Cardiovascular Research (Cre-lox System)
Cre-lox System (loxP-flanked Sequences)
Developmental Biology Research (Cre-lox System)
Hematological Research
Neurobiology Research
Selected Reference(s)
Additional ReferencesClarke SL; Vasanthakumar A; Anderson SA; Pondarre C; Koh CM; Deck KM; Pitula JS; Epstein CJ; Fleming MD; Eisenstein RS. 2006. Iron-responsive degradation of iron-regulatory protein 1 does not require the Fe-S cluster. EMBO J 25(3):544-53. [PubMed: 16424901] [MGI Ref ID J:105934]
| Strain Name: | B6.129S4-Abcb7tm1Mdf/J |
| Stock Number: | 006490 |
IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
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