| |||||||||||
| This strain carries a floxed allele of the platelet derived growth factor receptor alpha (Pdgfra). When used in conjunction with a strain expressing Cre recombinase in neural crest cells (dorsal neural tube), this strain may be useful in studies of cardiovascular and craniofacial development. | |||||||||||
Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Heterozygote x Heterozygote (Female x Male) Species laboratory mouse Generation N12+N9+F2 (30-DEC-08) Donating Investigator Philippe Soriano, Fred Hutchinson Cancer Research Center Description
These mice possess loxP sites on either side of exon 1 and exon 4 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele.For example, when bred to a strain expressing Cre recombinase in midbrain/dorsal spinal cord (see Stock No. 007807 for example), this mutant mouse strain may be useful in studies of cardiac neural crest cell migration.
Development
Exons 1 through 4 are flanked by loxP sites. A PGK-neo cassette was inserted downstream of exon 4. The embryonic stem cell line is not known. The resulting chimeric mice were bred to C57BL/6J mice for 12 generations and then bred to C57BL/6JEi for 9 generations. This targeted mutation strain was developed by Dr. Philippe Soriano at the Fred Hutchinson Cancer Research Center. Dr. Eva Eicher at The Jackson Laboratory obtained the strain from Dr. Soriano and backcrossed the mice to C57BL/6JEi.
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Strains carrying other alleles of Pdgfra
007669 B6.129S4-Pdgfratm11(EGFP)Sor/J View Strains carrying other alleles of Pdgfra (1 strain)
Cre-lox Systems
View Mammalian Phenotype Terms
Mammalian Phenotype Terms
assigned by genotype
The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.Pdgfratm8Sor/Pdgfratm8Sor Tg(Wnt1-cre)11Rth/0
Background Not Specified (conditional)
- lethality-postnatal
- postnatal lethality (MGI Ref ID J:81153)
- cardiovascular system phenotype
- abnormal patterning of the aortic arch (MGI Ref ID J:81153)
- observed between E17.7-E18.5 displayed
- abnormal vasculogenesis (MGI Ref ID J:81153)
- abnormal origin of the right subclavian artery
- abnormal ventricular septum morphology (MGI Ref ID J:81153)
- possible ventricular septal defects
- hemorrhage (MGI Ref ID J:81153)
- midline hemorrhage
- persistent truncus arteriosis (MGI Ref ID J:81153)
- observed between E17.7-E18.5 displayed
- craniofacial phenotype
- abnormal craniofacial morphology (MGI Ref ID J:81153)
- developmental defects appear at E11.5 with a gap in the frontal nasal processes and progresses to hemorrhaging and blebbing by E13.5
- abnormal frontal bone morphology (MGI Ref ID J:81153)
- phenotype varied in severity; the frontal bones were absent or failed to fuse
- abnormal nasal bone morphology (MGI Ref ID J:81153)
- phenotype varied in severity; the nasal bones were absent or failed to fuse
- abnormal nasal capsule morphology (MGI Ref ID J:81153)
- phenotype varied in severity; the nasal capsule was absent or failed to fuse
- midline facial cleft (MGI Ref ID J:81153)
- phenotype varied in severity due to defects in the nasal capsule and/or frontal and nasal bones
- short snout (MGI Ref ID J:81153)
- snouts shortened by 8%
- immune system phenotype
- small thymus (MGI Ref ID J:81153)
- observed with very low penetrance (3 out of 32 homozygous mutant embryos)
- skeleton phenotype
- abnormal frontal bone morphology (MGI Ref ID J:81153)
- phenotype varied in severity; the frontal bones were absent or failed to fuse
- abnormal nasal bone morphology (MGI Ref ID J:81153)
- phenotype varied in severity; the nasal bones were absent or failed to fuse
- abnormal nasal capsule morphology (MGI Ref ID J:81153)
- phenotype varied in severity; the nasal capsule was absent or failed to fuse
- hematopoietic system phenotype
- small thymus (MGI Ref ID J:81153)
- observed with very low penetrance (3 out of 32 homozygous mutant embryos)
- respiratory system phenotype
- abnormal nasal capsule morphology (MGI Ref ID J:81153)
- phenotype varied in severity; the nasal capsule was absent or failed to fuse
Pdgfratm8Sor/Pdgfratm8Sor Tg(Wnt1-cre)11Rth/?
involves: C57BL/6 * CBA (conditional)
- cardiovascular system phenotype
- abnormal heart development (MGI Ref ID J:122584)
- cardiac development is delayed
- persistent truncus arteriosis (MGI Ref ID J:122584)
- in 25% of mice
- abnormal ventricular septum morphology (MGI Ref ID J:122584)
- in 75% of mice
- retroesophageal right subclavian artery (MGI Ref ID J:122584)
- in 50% of mice
| Allele Symbol | Pdgfratm8Sor | ||
|---|---|---|---|
| Allele Name | targeted mutation 8, Philippe Soriano | ||
| Allele Type | Targeted (Floxed/Frt) | ||
| Common Name(s) | PDGFRalphafl; | ||
| Mutation Made By | Eva Eicher, The Jackson Laboratory | ||
| Gene Symbol and Name | Pdgfra, platelet derived growth factor receptor, alpha polypeptide | ||
| Chromosome | 5 | ||
| Gene Common Name(s) | AI115593; APDGFR; CD140A; MGC74795; PDGFACE; PDGFR2; Pdgfr-2; RHEPDGFRA; expressed sequence AI115593; | ||
| Molecular Note | A loxP site was introduced upstream of exon 1 and a PGK-neo-loxP cassette was introduced downstream of exon 4 via homologous recombination. [MGI Ref ID J:81153] | ||
Genotyping Protocols
Pdgfratm8Sor, STD PCR, vers. 1
Helpful Links
Genotyping resources and troubleshooting
Jackson EL; Garcia-Verdugo JM; Gil-Perotin S; Roy M; Quinones-Hinojosa A; VandenBerg S; Alvarez-Buylla A. 2006. PDGFR alpha-positive B cells are neural stem cells in the adult SVZ that form glioma-like growths in response to increased PDGF signaling. Neuron 51(2):187-99. [PubMed: 16846854] [MGI Ref ID J:115053]
Tallquist MD; Soriano P. 2003. Cell autonomous requirement for PDGFRalpha in populations of cranial and cardiac neural crest cells. Development 130(3):507-18. [PubMed: 12490557] [MGI Ref ID J:81153]
Pdgfratm8Sor relatedFrench WJ; Creemers EE; Tallquist MD. 2008. Platelet-derived growth factor receptors direct vascular development independent of vascular smooth muscle cell function. Mol Cell Biol 28(18):5646-57. [PubMed: 18606782] [MGI Ref ID J:140245]
Kang J; Gu Y; Li P; Johnson BL; Sucov HM; Thomas PS. 2008. PDGF-A as an epicardial mitogen during heart development. Dev Dyn 237(3):692-701. [PubMed: 18297729] [MGI Ref ID J:131554]
Pickett EA; Olsen GS; Tallquist MD. 2008. Disruption of PDGFR{alpha}-initiated PI3K activation and migration of somite derivatives leads to spina bifida. Development 135(3):589-98. [PubMed: 18192285] [MGI Ref ID J:131172]
Richarte AM; Mead HB; Tallquist MD. 2007. Cooperation between the PDGF receptors in cardiac neural crest cell migration. Dev Biol 306(2):785-96. [PubMed: 17499702] [MGI Ref ID J:122584]
Schmahl J; Rizzolo K; Soriano P. 2008. The PDGF signaling pathway controls multiple steroid-producing lineages. Genes Dev 22(23):3255-67. [PubMed: 19056881] [MGI Ref ID J:142042]
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice can be bred as homozygotes. Mating System Heterozygote x Heterozygote (Female x Male) Diet Information LabDiet® 5K52/5K67
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $171.30 Female or Male Homozygous for Pdgfratm8Sor
Pairs /Price (US dollars $) Pair Genotype $342.60 Homozygous for Pdgfratm8Sor x Homozygous for Pdgfratm8Sor
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $222.70 Female or Male Homozygous for Pdgfratm8Sor
Pairs /Price (US dollars $) Pair Genotype $445.40 Homozygous for Pdgfratm8Sor x Homozygous for Pdgfratm8Sor
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
|---|---|
| Supply Notes |
|
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Purchasing Information
JAX® Mice Orders
Surgical Services
Contact Information
Orders & Technical Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. In purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.