Description

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Heterozygote x Heterozygote         (Female x Male) Species laboratory mouse Generation N12+N9+F2 (30-DEC-08)   Donating Investigator Philippe Soriano,   Fred Hutchinson Cancer Research Center

Description
These mice possess loxP sites on either side of exon 1 and exon 4 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele.

For example, when bred to a strain expressing Cre recombinase in midbrain/dorsal spinal cord (see Stock No. 007807 for example), this mutant mouse strain may be useful in studies of cardiac neural crest cell migration.

Development
Exons 1 through 4 are flanked by loxP sites. A PGK-neo cassette was inserted downstream of exon 4. The embryonic stem cell line is not known. The resulting chimeric mice were bred to C57BL/6J mice for 12 generations and then bred to C57BL/6JEi for 9 generations. This targeted mutation strain was developed by Dr. Philippe Soriano at the Fred Hutchinson Cancer Research Center. Dr. Eva Eicher at The Jackson Laboratory obtained the strain from Dr. Soriano and backcrossed the mice to C57BL/6JEi.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Pdgfra

007669

B6.129S4-Pdgfratm11(EGFP)Sor/J

View Strains carrying other alleles of Pdgfra     (1 strain)

Additional Web Information

Cre-lox Systems

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

The following phenotype relates to a compound genotype created using this strain.
Contact JAX^® Services jaxservices@jax.org for customized breeding options.

Pdgfra^tm8Sor/Pdgfra^tm8Sor Tg(Wnt1-cre)11Rth/0

        Background Not Specified   (conditional)

• lethality-postnatal
• postnatal lethality (MGI Ref ID J:81153)
• cardiovascular system phenotype
• abnormal patterning of the aortic arch (MGI Ref ID J:81153)
  • observed between E17.7-E18.5 displayed
• abnormal vasculogenesis (MGI Ref ID J:81153)
  • abnormal origin of the right subclavian artery
• abnormal ventricular septum morphology (MGI Ref ID J:81153)
  • possible ventricular septal defects
• hemorrhage (MGI Ref ID J:81153)
  • midline hemorrhage
• persistent truncus arteriosis (MGI Ref ID J:81153)
  • observed between E17.7-E18.5 displayed
• craniofacial phenotype
• abnormal craniofacial morphology (MGI Ref ID J:81153)
  • developmental defects appear at E11.5 with a gap in the frontal nasal processes and progresses to hemorrhaging and blebbing by E13.5
• abnormal frontal bone morphology (MGI Ref ID J:81153)
  • phenotype varied in severity; the frontal bones were absent or failed to fuse
• abnormal nasal bone morphology (MGI Ref ID J:81153)
  • phenotype varied in severity; the nasal bones were absent or failed to fuse
• abnormal nasal capsule morphology (MGI Ref ID J:81153)
  • phenotype varied in severity; the nasal capsule was absent or failed to fuse
• midline facial cleft (MGI Ref ID J:81153)
  • phenotype varied in severity due to defects in the nasal capsule and/or frontal and nasal bones
• short snout (MGI Ref ID J:81153)
  • snouts shortened by 8%
• immune system phenotype
• small thymus (MGI Ref ID J:81153)
  • observed with very low penetrance (3 out of 32 homozygous mutant embryos)
• skeleton phenotype
• abnormal frontal bone morphology (MGI Ref ID J:81153)
  • phenotype varied in severity; the frontal bones were absent or failed to fuse
• abnormal nasal bone morphology (MGI Ref ID J:81153)
  • phenotype varied in severity; the nasal bones were absent or failed to fuse
• abnormal nasal capsule morphology (MGI Ref ID J:81153)
  • phenotype varied in severity; the nasal capsule was absent or failed to fuse
• hematopoietic system phenotype
• small thymus (MGI Ref ID J:81153)
  • observed with very low penetrance (3 out of 32 homozygous mutant embryos)
• respiratory system phenotype
• abnormal nasal capsule morphology (MGI Ref ID J:81153)
  • phenotype varied in severity; the nasal capsule was absent or failed to fuse

Pdgfra^tm8Sor/Pdgfra^tm8Sor Tg(Wnt1-cre)11Rth/?

        involves: C57BL/6 * CBA   (conditional)

• cardiovascular system phenotype
• abnormal heart development (MGI Ref ID J:122584)
  • cardiac development is delayed
• persistent truncus arteriosis (MGI Ref ID J:122584)
  • in 25% of mice
• abnormal ventricular septum morphology (MGI Ref ID J:122584)
  • in 75% of mice
• retroesophageal right subclavian artery (MGI Ref ID J:122584)
  • in 50% of mice

Genes & Alleles

Gene & Allele Information

Allele Symbol		Pdgfratm8Sor
Allele Name		targeted mutation 8, Philippe Soriano
Allele Type		Targeted (Floxed/Frt)
Common Name(s)		PDGFRalphafl;
Mutation Made By		Eva Eicher,   The Jackson Laboratory
Gene Symbol and Name		Pdgfra, platelet derived growth factor receptor, alpha polypeptide
Chromosome		5
Gene Common Name(s)		AI115593; APDGFR; CD140A; MGC74795; PDGFACE; PDGFR2; Pdgfr-2; RHEPDGFRA; expressed sequence AI115593;
Molecular Note		A loxP site was introduced upstream of exon 1 and a PGK-neo-loxP cassette was introduced downstream of exon 4 via homologous recombination. [MGI Ref ID J:81153]

Genotyping

Genotyping Information

Genotyping Protocols

Pdgfra^tm8Sor, STD PCR, vers. 1

Helpful Links

Genotyping resources and troubleshooting

References

References

Selected Reference(s)

Jackson EL; Garcia-Verdugo JM; Gil-Perotin S; Roy M; Quinones-Hinojosa A; VandenBerg S; Alvarez-Buylla A. 2006. PDGFR alpha-positive B cells are neural stem cells in the adult SVZ that form glioma-like growths in response to increased PDGF signaling. Neuron 51(2):187-99. [PubMed: 16846854]  [MGI Ref ID J:115053]

Tallquist MD; Soriano P. 2003. Cell autonomous requirement for PDGFRalpha in populations of cranial and cardiac neural crest cells. Development 130(3):507-18. [PubMed: 12490557]  [MGI Ref ID J:81153]

Additional References

Pdgfra^tm8Sor related

French WJ; Creemers EE; Tallquist MD. 2008. Platelet-derived growth factor receptors direct vascular development independent of vascular smooth muscle cell function. Mol Cell Biol 28(18):5646-57. [PubMed: 18606782]  [MGI Ref ID J:140245]

Kang J; Gu Y; Li P; Johnson BL; Sucov HM; Thomas PS. 2008. PDGF-A as an epicardial mitogen during heart development. Dev Dyn 237(3):692-701. [PubMed: 18297729]  [MGI Ref ID J:131554]

Pickett EA; Olsen GS; Tallquist MD. 2008. Disruption of PDGFR{alpha}-initiated PI3K activation and migration of somite derivatives leads to spina bifida. Development 135(3):589-98. [PubMed: 18192285]  [MGI Ref ID J:131172]

Richarte AM; Mead HB; Tallquist MD. 2007. Cooperation between the PDGF receptors in cardiac neural crest cell migration. Dev Biol 306(2):785-96. [PubMed: 17499702]  [MGI Ref ID J:122584]

Schmahl J; Rizzolo K; Soriano P. 2008. The PDGF signaling pathway controls multiple steroid-producing lineages. Genes Dev 22(23):3255-67. [PubMed: 19056881]  [MGI Ref ID J:142042]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX11

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, these mice can be bred as homozygotes.
Mating System	Heterozygote x Heterozygote         (Female x Male)
Diet Information	LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement.

Supply Notes

Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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