Strain Name:

B6.Cg-Pdgfratm8Sor/EiJ

Stock Number:

006492

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Availability:

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This strain carries a floxed allele of the platelet derived growth factor receptor alpha (Pdgfra). When used in conjunction with a strain expressing Cre recombinase in neural crest cells (dorsal neural tube), this strain may be useful in studies of cardiovascular and craniofacial development.

Description

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
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Additional information on Congenic nomenclature.
Mating SystemHomozygote x Homozygote         (Female x Male)   10-OCT-09
Specieslaboratory mouse
GenerationN12+N9F10 (11-DEC-13)
Generation Definitions
 
Donating InvestigatorDr. Philippe Soriano,   Mount Sinai School of Medicine

Description
These mice possess loxP sites on either side of exon 1 and exon 4 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele.

For example, when bred to a strain expressing Cre recombinase in midbrain/dorsal spinal cord (see Stock No. 007807 or 009107 for example), this mutant mouse strain may be useful in studies of cranial and cardiac neural crest cells.

Development
Exons 1 through 4 are flanked by loxP sites. A PGK-neo cassette was inserted downstream of exon 4. The embryonic stem cell line is not known. The resulting chimeric mice were bred to C57BL/6J mice for 12 generations and then bred to C57BL/6JEi for 9 generations. This targeted mutation strain was developed by Dr. Philippe Soriano at the Fred Hutchinson Cancer Research Center. Dr. Eva Eicher at The Jackson Laboratory obtained the strain from Dr. Soriano and backcrossed the mice to C57BL/6JEi.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Pdgfra
007669   B6.129S4-Pdgfratm11(EGFP)Sor/J
018433   B6.129S4-Pdgfratm12Sor/J
018280   B6N.Cg-Tg(Pdgfra-cre/ERT)467Dbe/J
013148   C57BL/6-Tg(Pdgfra-cre)1Clc/J
018432   STOCK Pdgfratm13Sor/J
View Strains carrying other alleles of Pdgfra     (5 strains)

Additional Web Information

Introduction to Cre-lox technology

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Gastrointestinal Stromal Tumor; GIST   (PDGFRA)
Hypereosinophilic Syndrome, Idiopathic; HES   (PDGFRA)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype relates to a compound genotype created using this strain.
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Pdgfratm8Sor/Pdgfratm8Sor Tg(Wnt1-cre)11Rth/0

        Background Not Specified   (conditional)
  • mortality/aging
  • postnatal lethality   (MGI Ref ID J:81153)
  • cardiovascular system phenotype
  • abnormal vasculogenesis
    • abnormal origin of the right subclavian artery   (MGI Ref ID J:81153)
  • hemorrhage
    • midline hemorrhage   (MGI Ref ID J:81153)
  • perimembraneous ventricular septal defect   (MGI Ref ID J:81153)
  • persistent truncus arteriosis
    • observed between E17.7-E18.5   (MGI Ref ID J:81153)
  • craniofacial phenotype
  • abnormal frontal bone morphology
    • phenotype varied in severity; the frontal bones were absent or failed to fuse   (MGI Ref ID J:81153)
  • abnormal frontonasal prominence morphology
    • developmental defects appear at E11.5 with a gap in the frontal nasal processes and progresses to hemorrhaging and blebbing by E13.5   (MGI Ref ID J:81153)
  • abnormal nasal bone morphology
    • phenotype varied in severity; the nasal bones were absent or failed to fuse   (MGI Ref ID J:81153)
  • abnormal nasal capsule morphology
    • phenotype varied in severity; the nasal capsule was absent or failed to fuse   (MGI Ref ID J:81153)
  • midline facial cleft
    • phenotype varied in severity due to defects in the nasal capsule and/or frontal and nasal bones   (MGI Ref ID J:81153)
  • short snout
    • snouts shortened by 8%   (MGI Ref ID J:81153)
  • immune system phenotype
  • small thymus
    • observed with very low penetrance (3 out of 32 homozygous mutant embryos)   (MGI Ref ID J:81153)
  • skeleton phenotype
  • abnormal frontal bone morphology
    • phenotype varied in severity; the frontal bones were absent or failed to fuse   (MGI Ref ID J:81153)
  • abnormal nasal bone morphology
    • phenotype varied in severity; the nasal bones were absent or failed to fuse   (MGI Ref ID J:81153)
  • abnormal nasal capsule morphology
    • phenotype varied in severity; the nasal capsule was absent or failed to fuse   (MGI Ref ID J:81153)
  • hematopoietic system phenotype
  • small thymus
    • observed with very low penetrance (3 out of 32 homozygous mutant embryos)   (MGI Ref ID J:81153)
  • respiratory system phenotype
  • abnormal nasal capsule morphology
    • phenotype varied in severity; the nasal capsule was absent or failed to fuse   (MGI Ref ID J:81153)

Pdgfratm8Sor/Pdgfratm8Sor Tg(Wnt1-cre)11Rth/?

        involves: C57BL/6 * CBA   (conditional)
  • cardiovascular system phenotype
  • delayed heart development
    • cardiac development is delayed   (MGI Ref ID J:122584)
  • persistent truncus arteriosis
    • in 25% of mice   (MGI Ref ID J:122584)
  • retroesophageal right subclavian artery
    • in 50% of mice   (MGI Ref ID J:122584)
  • ventricular septal defect
    • in 75% of mice   (MGI Ref ID J:122584)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Research Tools
Cre-lox System
      loxP-flanked Sequences

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Pdgfratm8Sor
Allele Name targeted mutation 8, Philippe Soriano
Allele Type Targeted (Floxed/Frt)
Common Name(s) PDGFRalphafl;
Mutation Made By Eva Eicher,   The Jackson Laboratory
Gene Symbol and Name Pdgfra, platelet derived growth factor receptor, alpha polypeptide
Chromosome 5
Gene Common Name(s) AI115593; APDGFR; CD140A; PDGFACE; PDGFR-2; PDGFR2; Pdgfr-2; RHEPDGFRA; expressed sequence AI115593;
Molecular Note A loxP site was introduced upstream of exon 1 and a PGK-neo-loxP cassette was introduced downstream of exon 4 via homologous recombination. [MGI Ref ID J:81153]

Genotyping

Genotyping Information

Genotyping Protocols

Pdgfratm8Sor, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Jackson EL; Garcia-Verdugo JM; Gil-Perotin S; Roy M; Quinones-Hinojosa A; VandenBerg S; Alvarez-Buylla A. 2006. PDGFR alpha-positive B cells are neural stem cells in the adult SVZ that form glioma-like growths in response to increased PDGF signaling. Neuron 51(2):187-99. [PubMed: 16846854]  [MGI Ref ID J:115053]

Tallquist MD; Soriano P. 2003. Cell autonomous requirement for PDGFRalpha in populations of cranial and cardiac neural crest cells. Development 130(3):507-18. [PubMed: 12490557]  [MGI Ref ID J:81153]

Additional References

Pdgfratm8Sor related

Abraham J; Chua YX; Glover JM; Tyner JW; Loriaux MM; Kilcoyne A; Giles FJ; Nelon LD; Carew JS; Ouyang Y; Michalek JE; Pal R; Druker BJ; Rubin BP; Keller C. 2012. An adaptive Src-PDGFRA-Raf axis in rhabdomyosarcoma. Biochem Biophys Res Commun 426(3):363-8. [PubMed: 22960170]  [MGI Ref ID J:190138]

Baek ST; Tallquist MD. 2012. Nf1 limits epicardial derivative expansion by regulating epithelial to mesenchymal transition and proliferation. Development 139(11):2040-9. [PubMed: 22535408]  [MGI Ref ID J:183991]

Bleyl SB; Saijoh Y; Bax NA; Gittenberger-de Groot AC; Wisse LJ; Chapman SC; Hunter J; Shiratori H; Hamada H; Yamada S; Shiota K; Klewer SE; Leppert MF; Schoenwolf GC. 2010. Dysregulation of the PDGFRA gene causes inflow tract anomalies including TAPVR: integrating evidence from human genetics and model organisms. Hum Mol Genet 19(7):1286-301. [PubMed: 20071345]  [MGI Ref ID J:157946]

Chen H; Gu X; Liu Y; Wang J; Wirt SE; Bottino R; Schorle H; Sage J; Kim SK. 2011. PDGF signalling controls age-dependent proliferation in pancreatic beta-cells. Nature 478(7369):349-55. [PubMed: 21993628]  [MGI Ref ID J:177411]

French WJ; Creemers EE; Tallquist MD. 2008. Platelet-derived growth factor receptors direct vascular development independent of vascular smooth muscle cell function. Mol Cell Biol 28(18):5646-57. [PubMed: 18606782]  [MGI Ref ID J:140245]

Greif DM; Kumar M; Lighthouse JK; Hum J; An A; Ding L; Red-Horse K; Espinoza FH; Olson L; Offermanns S; Krasnow MA. 2012. Radial construction of an arterial wall. Dev Cell 23(3):482-93. [PubMed: 22975322]  [MGI Ref ID J:189012]

He F; Soriano P. 2013. A Critical Role for PDGFRalpha Signaling in Medial Nasal Process Development. PLoS Genet 9(9):e1003851. [PubMed: 24086166]  [MGI Ref ID J:202450]

Kang J; Gu Y; Li P; Johnson BL; Sucov HM; Thomas PS. 2008. PDGF-A as an epicardial mitogen during heart development. Dev Dyn 237(3):692-701. [PubMed: 18297729]  [MGI Ref ID J:131554]

Macleod RJ. 2013. Extracellular calcium-sensing receptor/PTH knockout mice colons have increased Wnt/beta-catenin signaling, reduced non-canonical Wnt signaling, and increased susceptibility to azoxymethane-induced aberrant crypt foci. Lab Invest 93(5):520-7. [PubMed: 23545937]  [MGI Ref ID J:196174]

McGowan SE; McCoy DM. 2013. Platelet-derived growth factor-A regulates lung fibroblast S-phase entry through p27kip1 and FoxO3a. Respir Res 14:68. [PubMed: 23819440]  [MGI Ref ID J:199141]

Nakamura Y; Inloes JB; Katagiri T; Kobayashi T. 2011. Chondrocyte-Specific MicroRNA-140 Regulates Endochondral Bone Development and Targets Dnpep To Modulate Bone Morphogenetic Protein Signaling. Mol Cell Biol 31(14):3019-28. [PubMed: 21576357]  [MGI Ref ID J:174096]

Pickett EA; Olsen GS; Tallquist MD. 2008. Disruption of PDGFR{alpha}-initiated PI3K activation and migration of somite derivatives leads to spina bifida. Development 135(3):589-98. [PubMed: 18192285]  [MGI Ref ID J:131172]

Richarte AM; Mead HB; Tallquist MD. 2007. Cooperation between the PDGF receptors in cardiac neural crest cell migration. Dev Biol 306(2):785-96. [PubMed: 17499702]  [MGI Ref ID J:122584]

Schmahl J; Rizzolo K; Soriano P. 2008. The PDGF signaling pathway controls multiple steroid-producing lineages. Genes Dev 22(23):3255-67. [PubMed: 19056881]  [MGI Ref ID J:142042]

Smith CL; Baek ST; Sung CY; Tallquist MD. 2011. Epicardial-derived cell epithelial-to-mesenchymal transition and fate specification require PDGF receptor signaling. Circ Res 108(12):e15-26. [PubMed: 21512159]  [MGI Ref ID J:185693]

Zhou YX; Pannu R; Le TQ; Armstrong RC. 2012. Fibroblast growth factor 1 (FGFR1) modulation regulates repair capacity of oligodendrocyte progenitor cells following chronic demyelination. Neurobiol Dis 45(1):196-205. [PubMed: 21854849]  [MGI Ref ID J:179847]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX10

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice can be bred as homozygotes.
Mating SystemHomozygote x Homozygote         (Female x Male)   10-OCT-09
Diet Information LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $232.00Female or MaleHomozygous for Pdgfratm8Sor  
Price per Pair (US dollars $)Pair Genotype
$464.00Homozygous for Pdgfratm8Sor x Homozygous for Pdgfratm8Sor  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $301.60Female or MaleHomozygous for Pdgfratm8Sor  
Price per Pair (US dollars $)Pair Genotype
$603.20Homozygous for Pdgfratm8Sor x Homozygous for Pdgfratm8Sor  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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