Strain Name: |
B6;129-Trp53bp1tm1Jc/J |
|---|---|
Stock Number: |
006495 |
Availability: | Repository- Live |
General Terms and Conditions |
| Genes & Alleles | Trp53bp1; Trp53bp1tm1Jc; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Targeted Mutation Mating System Heterozygote x Heterozygote (Female x Male) Species laboratory mouse Donating Investigator Junjie Chen, Mayo Clinic College of Medicine Generation N1+N1pN1+F1 (11-APR-08) Strain Description
Homozygous "53BP1"-deficient mice are viable and fertile, but exhibit retarded growth and generate reduced litter sizes. Protein from the targeted gene is not detected in the testes (by immunoblot) or in mouse embryonic fibroblasts (MEFs) (by immunofluorescence). Homozygotes are immunocompromised, hypersensitive to whole-body irradiation, and develop thymic lymphomas with higher frequency (8%) compared to wildtype by 4-7 months of age. MEFs from homozygous mutant mice have a defective DNA damage response with impaired Chk2 activation. These mutant mice may be useful in studies of the immune system, cancer, tumor suppression, and DNA damage response pathways.Strain Development
A targeting vector containing a neomycin resistance gene was used to replace the exon spanning nucleotides 3777 to 4048 of the endogenous gene. The construct was electroporated into "129/SvE"-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and chimeric males were mated to C57BL/6 females. The resulting heterozygous mice were interbred for many generations prior to arrival at The Jackson Laboratory.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Trp53bp1tm1Jc | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Junjie Chen | ||
| Common Name(s) | 53BP1-; | ||
| Mutation Made By | Kay Minn, Mayo Clinic College of Medicine | ||
| Strain of Origin | 129/Sv | ||
| Gene Symbol and Name | Trp53bp1, transformation related protein 53 binding protein 1 | ||
| Chromosome | 2 | ||
| Gene Common Name(s) | 53BP1; FLJ41424; MGC138366; p202; | ||
| Molecular Note | The gene was disrupted by replacement of an exon containing nucleotides 3777 - 4048 with a neomycin resistance cassette via homologous recombination. Absence of gene expression in homozygous mutant animals was confirmed by Western blot analysis using an antibody directed against the N-terminal portion of the protein. [MGI Ref ID J:82512] | ||
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 101043 B6129SF1/J | (approximate) | |
| 101045 B6129SF2/J | (approximate) | |
| Considerations for Choosing Controls | ||
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, homozygous mice are bred together. Homozygotes have an increased frequency (approximately 8%) of thymic tumors between 4-7 months of age. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying other alleles of Trp53bp1
005375 STOCK Trp53bp1hlb543/CloJ View Strains carrying other alleles of Trp53bp1 (1 strain)
Animal Health Reports
Room Number AX12
Research Applications
This mouse can be used to support research in many areas including:
Cancer Research
Increased Tumor Incidence (Lymphomas: thymic)
Other (DNA Repair)
Cell Biology Research
DNA Damage Response
Genes Regulating Growth and Proliferation
Developmental Biology Research
Growth Defects
Immunology and Inflammation Research
Immunodeficiency (multiple immune defects)
Immunodeficiency
Immunodeficiency Associated with Other Defects
Internal/Organ Research
Thymus Defects
Research Tools
Cancer Research
Cell Biology Research
Immunology and Inflammation Research
References
Selected Reference(s)
Additional ReferencesWard IM; Minn K; van Deursen J; Chen J. 2003. p53 Binding protein 53BP1 is required for DNA damage responses and tumor suppression in mice. Mol Cell Biol 23(7):2556-63. [PubMed: 12640136] [MGI Ref ID J:82512]
Price and Supply Information
| Strain Name: | B6;129-Trp53bp1tm1Jc/J |
| Stock Number: | 006495 |
Price Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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