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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation N7+N1F2pN1 Donating Investigator Sonia Pearson-White, Description
Mice homozygous for this targeted mutation (called "Snoex1" in the primary reference) are viable and fertile with no reported gross morphological defects. Although the deletion of exon 1 leads to complete absence of the mature full-length protein in immunoblots of brain and embryonic tissues, a truncated 3'-end RNA species is derived from downstream coding sequence. Homozygotes exhibit T cell proliferation/activation defects, which can be rescued by treatment with anti-TGF-beta antibodies or exogenous interleukin-2. Homozygous deletion also results in increased sensitivity to TGF-beta and altered growth properties of cultured mouse embryo fibroblasts (MEFs). These mutant mice may be useful in studies of T cell activation, T cell receptor stimulation and TGF-beta signaling.Development
A targeting vector was designed to replace exon 1 of the endogenous gene with a lacZ gene (with a nuclear localization signal) and a loxP-flanked PGK-neo cassette. This construct was microinjected into (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts, which were then implanted into pseudopregnant foster mothers, resulting in heterozygous offspring. Prior to their arrival at The Jackson Laboratory these mice were backcrossed to C57BL/6 for seven generations.
Control Information
| Control | ||
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| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
lacZ Expression Strains
View lacZ Expression Strains (186 strains)
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Skiltm2Spw/Skiltm2Spw
either: (involves: 129S1/Sv * 129X1/SvJ) or (involves: 129S1/Sv * 129X1/SvJ * C57BL/6)
- tumorigenesis
- *normal* tumorigenesis (MGI Ref ID J:84554)
- no significant increase in the incidence of somatic tumors up to 2 years of age
- immune system phenotype
- *normal* immune system phenotype (MGI Ref ID J:84554)
- T-cell subpopulations from thymus and spleen unchanged from wild type T-cell subpopulations from thymus and spleen unchanged from wild-type
- decreased T cell proliferation (MGI Ref ID J:84554)
- reduced T-cell activation in response to receptor stimulation
- proliferation was normal when stimulation bypassed early steps in activation
- IL-2 reverses proliferation defects
- effect probably due in part to reduced antagonism of TGF-beta signaling
- hematopoietic system phenotype
- decreased T cell proliferation (MGI Ref ID J:84554)
- reduced T-cell activation in response to receptor stimulation
- proliferation was normal when stimulation bypassed early steps in activation
- IL-2 reverses proliferation defects
- effect probably due in part to reduced antagonism of TGF-beta signaling
This mouse can be used to support research in many areas including:
Cell Biology Research
Genes Regulating Growth and Proliferation
Immunology and Inflammation Research
Growth Factors/Receptors/Cytokines
Immunodeficiency
T cell deficiency
Internal/Organ Research
Lymphoid Tissue Defects
T cell deficiency
Research Tools
lacZ Expression
Cancer Research
T cell deficiency
Cell Biology Research
Immunology and Inflammation Research
T cell deficiency
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Skiltm2Spw | ||
|---|---|---|---|
| Allele Name | targeted mutation 2, Sonia Pearson-White | ||
| Allele Type | Targeted (Reporter) | ||
| Common Name(s) | Snoex1-/-; | ||
| Mutation Made By | Sonia Pearson-White, | ||
| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| ES Cell Line Name | R1 | ||
| ES Cell Line Strain | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| Site of Expression | lacZ expression patterns have not been characterized. | ||
| Gene Symbol and Name | Skil, SKI-like | ||
| Chromosome | 3 | ||
| Gene Common Name(s) | 9130011J04Rik; RIKEN cDNA 9130011J04 gene; SNO; Skir; SnoA; SnoI; SnoN; SnoN2; ski/sno related; sno-dE3; | ||
| Molecular Note | Exon 1 was replaced with a lacZ gene together with a floxed neomycin gene construct. Full length mRNA transcript is reduced below detectable levels in homozygotes although truncated mRNA was produced. No full length protein from these mRNAs was detectable on immunoblots. [MGI Ref ID J:84554] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Skiltm2Spw, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Pearson-White S; McDuffie M. 2003. Defective T-cell activation is associated with augmented transforming growth factor Beta sensitivity in mice with mutations in the Sno gene. Mol Cell Biol 23(15):5446-59. [PubMed: 12861029] [MGI Ref ID J:84554]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice may be bred as homozygotes.
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Animals Provided
Price (US dollars $) Cryorecovery Fee $1900.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
| Pricing for International shipping destinations |
|
Animals Provided
Price (US dollars $) Cryorecovery Fee $2470.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
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Supply Details
| Standard Supply | Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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For Licensing and Use Restrictions view the link(s) below:
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Contact information
General inquiries
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| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
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