Strain Name: |
B6.129-Skiltm2Spw/J |
|---|---|
Stock Number: |
006497 |
Availability: | Repository- Live |
Price and Supply Information | |
General Terms and Conditions |
| Genes & Alleles | Skil; Skiltm2Spw; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Donating Investigator Sonia Pearson-White, Generation N7+N1F2 (03-DEC-07) Strain Description
Mice homozygous for this targeted mutation (called "Snoex1" in the primary reference) are viable and fertile with no reported gross morphological defects. Although the deletion of exon 1 leads to complete absence of the mature full-length protein in immunoblots of brain and embryonic tissues, a truncated 3'-end RNA species is derived from downstream coding sequence. Homozygotes exhibit T cell proliferation/activation defects, which can be rescued by treatment with anti-TGF-beta antibodies or exogenous interleukin-2. Homozygous deletion also results in increased sensitivity to TGF-beta and altered growth properties of cultured mouse embryo fibroblasts (MEFs). These mutant mice may be useful in studies of T cell activation, T cell receptor stimulation and TGF-beta signaling.Strain Development
A targeting vector was designed to replace exon 1 of the endogenous gene with a lacZ gene (with a nuclear localization signal) and a loxP-flanked PGK-neo cassette. This construct was microinjected into (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts, which were then implanted into pseudopregnant foster mothers, resulting in heterozygous offspring. Prior to their arrival at The Jackson Laboratory these mice were backcrossed to C57BL/6 for seven generations.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Skiltm2Spw | ||
|---|---|---|---|
| Allele Name | targeted mutation 2, Sonia Pearson-White | ||
| Common Name(s) | Snoex1-/-; | ||
| Mutation Made By | Sonia Pearson-White, | ||
| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ | ||
| ES Cell Line Name | R1 | ||
| ES Cell Line Strain | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| Site of Expression | lacZ expression patterns have not been characterized. | ||
| Gene Symbol and Name | Skil, SKI-like | ||
| Chromosome | 3 | ||
| Gene Common Name(s) | 9130011J04Rik; RIKEN cDNA 9130011J04 gene; SNO; Skir; SnoA; SnoN; SnoN2; ski/sno related; sno-dE3; | ||
| Molecular Note | Exon 1 was replaced with a lacZ gene together with a floxed neomycin gene construct. Full length mRNA transcript is reduced below detectable levels in homozygotes although truncated mRNA was produced. No full length protein from these mRNAs was detectable on immunoblots. [MGI Ref ID J:84554] | ||
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for JAX® GEMM® Strains | ||
Genotyping Protocols
Skiltm2Spw
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, these mice may be bred as homozygotes. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
lacZ Expression Strains
View lacZ Expression Strains (174 strains)
Additional Web Information
Congenic Nomenclature
Fluorescent Proteins/lacZ Systems
Animal Health Reports
Room Number AX11
Research Applications
This mouse can be used to support research in many areas including:
Cell Biology Research
Genes Regulating Growth and Proliferation
Immunology and Inflammation Research
Growth Factors/Receptors/Cytokines
Immunodeficiency (T cell deficiency)
Internal/Organ Research
Lymphoid Tissue Defects (T cell deficiency)
Research Tools
lacZ Expression
Cancer Research (T cell deficiency)
Cell Biology Research
Immunology and Inflammation Research (T cell deficiency)
References
Selected Reference(s)
Pearson-White S; McDuffie M. 2003. Defective T-cell activation is associated with augmented transforming growth factor Beta sensitivity in mice with mutations in the Sno gene. Mol Cell Biol 23(15):5446-59. [PubMed: 12861029] [MGI Ref ID J:84554]
Price and Supply Information
| Strain Name: | B6.129-Skiltm2Spw/J |
| Stock Number: | 006497 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. The shipping destinations are:
- USA, Canada and Mexico
- International
*Pricing for Shipping Destination selected:
USA, Canada and Mexico
| Price(s) in US dollars ($) | Genotype(s) Provided | ||||
|---|---|---|---|---|---|
| Individual Mouse Price | $155.70 | Homozygous for Skiltm2Spw | |||
| Pair | $311.40 | Homozygous for Skiltm2Spw x Homozygous for Skiltm2Spw | |||
Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Histology and Tissue Collection Services are available for all JAX® Mice strains. For more information, please contact Customer Service at orderquest@jax.org or 1-800-422-6423. Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. View Control Pricing Information for JAX® Strains. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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