Strain Name:

B6SJL-Tg(APPSwFlLon,PSEN1*M146L*L286V)6799Vas/Mmjax

Availability:

Repository- Live     Available at the JAX MMRRC

Use Restrictions Apply, see Terms of Use
This strain is distributed by the Mutant Mouse Regional Resource Center. Please refer to the Mutant Mouse Regional Resource Center (MMRRC) for ordering information and strain details on B6SJL-Tg(APPSwFlLon,PSEN1*M146L*L286V)6799Vas/Mmjax MMRRC Stock Number 034840.
As a designated MMRRC center, The Jackson Laboratory will continue to distribute these mice at the same high health and
quality standards but ordering is exclusively provided through the MMRRC.
These 5XFAD transgenic mice (formerly JAX Stock No. 006554) overexpress mutant human APP(695) with the Swedish (K670N, M671L), Florida (I716V), and London (V717I) Familial Alzheimer's Disease (FAD) mutations along with human PS1 harboring two FAD mutations, M146L and L286V. Both transgenes are regulated by the mouse Thy1 promoter to drive overexpression in the brain. 5XFAD mice recapitulate major features of Alzheimer's Disease amyloid pathology and may be a useful model of intraneuronal Abeta-42 induced neurodegeneration and amyloid plaque formation.

Description

Strain Information

Former Names B6SJL-Tg(APPSwFlLon,PSEN1*M146L*L286V)6799Vas/J    (Changed: 11-AUG-11 )
Type Mutant Stock; Transgenic;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Mating SystemHemizygote x F1         (Female x Male)   28-OCT-13
Mating SystemF1 x Hemizygote         (Female x Male)   28-OCT-13
Specieslaboratory mouse
GenerationN11+N13 (10-DEC-13)
Generation Definitions
 
Donating Investigator Robert Vassar,   Northwestern University

Description
These 5XFAD transgenic mice overexpress both mutant human APP(695) with the Swedish (K670N, M671L), Florida (I716V), and London (V717I) Familial Alzheimer's Disease (FAD) mutations and human PS1 harboring two FAD mutations, M146L and L286V. Expression of both transgenes is regulated by neural-specific elements of the mouse Thy1 promoter to drive overexpression in the brain. Mice from this founder line have high APP expression correlating with high burden and accelerated accumulation of the 42 amino acid species of beta-amyloid (Abeta-42). 5XFAD mice generate Abeta-42 almost exclusively, rapidly accumulating massive cerebral levels. On the B6SJL F1 genetic background (see MMRRC stock 34840, intraneuronal Abeta-42 accumulation is observed starting at 1.5 months of age, just prior to amyloid deposition and gliosis, which begins at two months of age. On a congenic C57BL/6J genetic background (see MMRRC stock 34848) it has been the observation of the MMRRC that this phenotype is not as robust as that demonstrated in the B6SJL hybrid background. In addition, these mice have reduced synaptic marker protein levels, increased p25 levels, neuron loss, and memory impairment in the Y-maze test. 5XFAD transgenic mice recapitulate major features of Alzheimer's Disease amyloid pathology and may be useful models of intraneuronal Abeta-42 induced neurodegeneration and amyloid plaque formation. Hemizygous mice are viable and fertile.

This strain is segregating heterozygous/wildtype for the retinal degeneration allele Pde6brd1.

Development
A transgene was designed with a mutant human amyloid beta (A4) precursor protein (APP) cDNA sequence (altered to include the APP K670N/M671L (Swedish) + I716V (Florida) + V717I (London) Familial Alzheimer's Disease (FAD) mutations) inserted into exon 2 of the mouse Thy1 gene. A second transgene was designed with a mutant human presenilin 1 (Alzheimer disease 3) (PSEN1 or PS1) cDNA sequence (altered to include the PS1 M146L + L286V FAD mutations) inserted into exon 2 of the mouse Thy1 gene. Both transgenes were added together in equal proportions and co-injected into the pronuclei of single-cell "C57/B6XSJL" hybrid embryos. Founders from the highest APP expressing line (Tg6799) were bred with (B6/SJL)F1 for more than 10 generations with stable germ-line transmission and expression of both transgenes, demonstrating that these "5XFAD"; mice breed as single transgenics. Of note, the APP transgene includes the 5' untranslated region and thus contains a putative interleukin-1beta translational enhancer element.

Control Information

  Control
   Noncarrier
   100012 B6SJLF1/J
 
  Considerations for Choosing Controls

Related Strains

Alzheimer's Disease Models
005987   129-Achetm1Loc/J
006409   129S1.129(Cg)-Tg(APPSw)40Btla/Mmjax
008077   129S1/Sv-Bchetm1Loc/J
016198   129S6.Cg-Tg(Camk2a-tTA)1Mmay/JlwsJ
014556   129S6/SvEv-Apoetm4Mae/J
006555   A.129(B6)-Tg(APPSw)40Btla/Mmjax
005708   B6.129-Apbb1tm1Quhu/J
004714   B6.129-Bace1tm1Pcw/J
004098   B6.129-Klc1tm1Gsn/J
004193   B6.129-Psen1tm1Mpm/J
003615   B6.129-Psen1tm1Shn/J
005300   B6.129-Tg(APPSw)40Btla/Mmjax
005617   B6.129P-Psen2tm1Bdes/J
002609   B6.129P2-Nos2tm1Lau/J
007685   B6.129P2-Psen1tm1Vln/J
007999   B6.129P2-Sorl1Gt(Ex255)Byg/J
008087   B6.129S1-Bchetm1Loc/J
002509   B6.129S2-Plautm1Mlg/J
005301   B6.129S2-Tg(APP)8.9Btla/J
004163   B6.129S4-Cdk5r1tm1Lht/J
010959   B6.129S4-Grk5tm1Rjl/J
010960   B6.129S4-Grk5tm2Rjl/J
002213   B6.129S4-Ngfrtm1Jae/J
006406   B6.129S4-Tg(APPSwLon)96Btla/Mmjax
006469   B6.129S4-Tg(PSEN1H163R)G9Btla/J
012564   B6.129S5-Dhcr24tm1Lex/SbpaJ
004142   B6.129S7-Aplp2tm1Dbo/J
004133   B6.129S7-Apptm1Dbo/J
007251   B6.129X1-Mapttm1Hnd/J
013040   B6.Cg-Apoetm1Unc Ins2Akita/J
005642   B6.Cg-Clutm1Jakh/J
005491   B6.Cg-Mapttm1(EGFP)Klt Tg(MAPT)8cPdav/J
009126   B6.Cg-Nos2tm1Lau Tg(Thy1-APPSwDutIowa)BWevn/Mmjax
005866   B6.Cg-Tg(APP695)3Dbo Tg(PSEN1dE9)S9Dbo/Mmjax
008730   B6.Cg-Tg(APPSwFlLon,PSEN1*M146L*L286V)6799Vas/Mmjax
005864   B6.Cg-Tg(APPswe,PSEN1dE9)85Dbo/Mmjax
007575   B6.Cg-Tg(CAG-Ngb,-EGFP)1Dgrn/J
016197   B6.Cg-Tg(CAG-OTC/CAT)4033Prab/J
005855   B6.Cg-Tg(Camk2a-Prkaca)426Tabe/J
007004   B6.Cg-Tg(Camk2a-tTA)1Mmay/DboJ
004996   B6.Cg-Tg(DBH-Gal)1923Stei/J
007673   B6.Cg-Tg(Gad1-EGFP)3Gfng/J
004662   B6.Cg-Tg(PDGFB-APP)5Lms/J
006293   B6.Cg-Tg(PDGFB-APPSwInd)20Lms/2Mmjax
006006   B6.Cg-Tg(Prnp-APP)A-2Dbo/J
008596   B6.Cg-Tg(Prnp-Abca1)EHol/J
006005   B6.Cg-Tg(Prnp-App/APPswe)E1-2Dbo/Mmjax
007180   B6.Cg-Tg(Prnp-ITM2B/APP695*40)1Emcg/J
007182   B6.Cg-Tg(Prnp-ITM2B/APP695*42)A12Emcg/J
005999   B6.Cg-Tg(SBE/TK-luc)7Twc/J
012597   B6.Cg-Tg(Thy1-COL25A1)861Yfu/J
007051   B6.Cg-Tg(tetO-APPSwInd)102Dbo/Mmjax
007052   B6.Cg-Tg(tetO-APPSwInd)107Dbo/Mmjax
007049   B6.Cg-Tg(tetO-APPSwInd)885Dbo/Mmjax
009337   B6.FVB-Tg(Prnp-RTN3)2Yanr/J
006394   B6;129-Apba2tm1Sud Apba3tm1Sud Apba1tm1Sud/J
008364   B6;129-Chattm1(cre/ERT)Nat/J
008476   B6;129-Ncstntm1Sud/J
004807   B6;129-Psen1tm1Mpm Tg(APPSwe,tauP301L)1Lfa/Mmjax
007605   B6;129P-Psen1tm1Vln/J
005618   B6;129P2-Bace2tm1Bdes/J
008333   B6;129P2-Dldtm1Ptl/J
002596   B6;129P2-Nos2tm1Lau/J
003822   B6;129S-Psen1tm1Shn/J
012639   B6;129S4-Mapttm3(HDAC2)Jae/J
012869   B6;129S6-Apbb2tm1Her/J
006410   B6;129S6-Chattm2(cre)Lowl/J
005993   B6;129S6-Pcsk9tm1Jdh/J
008636   B6;C-Tg(Prnp-APP695*/EYFP)49Gsn/J
007002   B6;C3-Tg(Prnp-ITM2B/APP695*42)A12Emcg/Mmjax
008169   B6;C3-Tg(Prnp-MAPT*P301S)PS19Vle/J
000231   B6;C3Fe a/a-Csf1op/J
008850   B6;SJL-Tg(Mt1-LDLR)93-4Reh/AgnJ
003378   B6C3-Tg(APP695)3Dbo Tg(PSEN1)5Dbo/J
004462   B6C3-Tg(APPswe,PSEN1dE9)85Dbo/Mmjax
003741   B6D2-Tg(Prnp-MAPT)43Vle/J
016556   B6N.129-Ptpn5tm1Pjlo/J
018957   B6N.129S6(B6)-Chattm2(cre)Lowl/J
024841   B6N.Cg-Tg(Prnp-MAPT*P301S)PS19Vle/J
012621   C.129S(B6)-Chrna3tm1.1Hwrt/J
002328   C.129S2-Plautm1Mlg/J
003375   C3B6-Tg(APP695)3Dbo/Mmjax
005087   C57BL/6-Tg(Camk2a-IDE)1Selk/J
005086   C57BL/6-Tg(Camk2a-MME)3Selk/J
008833   C57BL/6-Tg(Camk2a-UBB)3413-1Fwvl/J
007027   C57BL/6-Tg(Thy1-APPSwDutIowa)BWevn/Mmjax
010800   C57BL/6-Tg(Thy1-PTGS2)300Kand/J
010703   C57BL/6-Tg(Thy1-PTGS2)303Kand/J
005706   C57BL/6-Tg(tetO-CDK5R1/GFP)337Lht/J
006618   C57BL/6-Tg(tetO-COX8A/EYFP)1Ksn/J
007677   CB6-Tg(Gad1-EGFP)G42Zjh/J
007072   CByJ.129P2(B6)-Nos2tm1Lau/J
006472   D2.129(B6)-Tg(APPSw)40Btla/Mmjax
007067   D2.129P2(B6)-Apoetm1Unc/J
013719   D2.Cg-Apoetm1Unc Ins2Akita/J
003718   FVB-Tg(GadGFP)45704Swn/J
013732   FVB-Tg(NPEPPS)1Skar/J
013156   FVB-Tg(tetO-CDK5R1*)1Vln/J
015815   FVB-Tg(tetO-MAPT*P301L)#Kha/JlwsJ
002329   FVB.129S2-Plautm1Mlg/J
003753   FVB/N-Tg(Eno2CDK5R1)1Jdm/J
006143   FVB/N-Tg(Thy1-cre)1Vln/J
008051   NOD.129P2(B6)-Ctsbtm1Jde/RclJ
008390   STOCK Apptm1Sud/J
012640   STOCK Hdac2tm1.2Rdp/J
004808   STOCK Mapttm1(EGFP)Klt Tg(MAPT)8cPdav/J
004779   STOCK Mapttm1(EGFP)Klt/J
014092   STOCK Tg(ACTB-tTA2,-MAPT/lacZ)1Luo/J
014544   STOCK Tg(tetO-ABL1*P242E*P249E)CPdav/J
View Alzheimer's Disease Models     (109 strains)

Strains carrying   Tg(APPSwFlLon,PSEN1*M146L*L286V)6799Vas allele
008730   B6.Cg-Tg(APPSwFlLon,PSEN1*M146L*L286V)6799Vas/Mmjax
View Strains carrying   Tg(APPSwFlLon,PSEN1*M146L*L286V)6799Vas     (1 strain)

View Strains carrying other alleles of APP695     (9 strains)

View Strains carrying other alleles of PSEN1     (5 strains)

Strains carrying other alleles of Thy1
005895   B10.Cg-Thy1a H2d Tg(TcraCl1,TcrbCl1)1Shrm/J
001317   B6.Cg-Gpi1a Thy1a Igha/J
017798   B6.Cg-Mapttm1Hnd Tg(Thy1-MAPT*)3610Gds/Mmjax
009126   B6.Cg-Nos2tm1Lau Tg(Thy1-APPSwDutIowa)BWevn/Mmjax
022073   B6.Cg-Rag1tm1Mom Thy1a Tg(Tcra2C,Tcrb2C)1Dlo/J
014550   B6.Cg-Thy1a Tg(TcraCWM5,TcrbCWM5)1807Wuth/J
005023   B6.Cg-Thy1a/Cy Tg(TcraTcrb)8Rest/J
007901   B6.Cg-Tg(Thy1-Brainbow1.0)HLich/J
007911   B6.Cg-Tg(Thy1-Brainbow1.1)MLich/J
007921   B6.Cg-Tg(Thy1-Brainbow2.1)RLich/J
003710   B6.Cg-Tg(Thy1-CFP)23Jrs/J
014131   B6.Cg-Tg(Thy1-CFP)IJrs/GfngJ
007940   B6.Cg-Tg(Thy1-CFP/COX8A)C1Lich/J
007967   B6.Cg-Tg(Thy1-CFP/COX8A)S2Lich/J
012597   B6.Cg-Tg(Thy1-COL25A1)861Yfu/J
007612   B6.Cg-Tg(Thy1-COP4/EYFP)18Gfng/J
007615   B6.Cg-Tg(Thy1-COP4/EYFP)9Gfng/J
013161   B6.Cg-Tg(Thy1-Clomeleon)1Gjau/J
007919   B6.Cg-Tg(Thy1-EGFP)OJrs/GfngJ
005630   B6.Cg-Tg(Thy1-EYFP)15Jrs/J
009611   B6.Cg-Tg(Thy1-Nlgn1)6Hnes/J
009612   B6.Cg-Tg(Thy1-Nlgn2)6Hnes/J
021069   B6.Cg-Tg(Thy1-PA-GFP)5Rmpl/J
021070   B6.Cg-Tg(Thy1-PA-GFP)6Rmpl/J
003709   B6.Cg-Tg(Thy1-YFP)16Jrs/J
003782   B6.Cg-Tg(Thy1-YFP)HJrs/J
005627   B6.Cg-Tg(Thy1-YFP/Syp)10Jrs/J
007606   B6.Cg-Tg(Thy1-cre/ERT2,-EYFP)AGfng/J
025854   B6.FVB-Ptprca Tg(CAG-luc,-GFP)L2G85Chco Thy1a/J
000406   B6.PL-Thy1a/CyJ
000983   B6.PL/(84NS)CyJ
004807   B6;129-Psen1tm1Mpm Tg(APPSwe,tauP301L)1Lfa/Mmjax
007910   B6;CBA-Tg(Thy1-Brainbow1.0)LLich/J
011070   B6;CBA-Tg(Thy1-EGFP)SJrs/NdivJ
017892   B6;CBA-Tg(Thy1-GCaMP2.2c)8Gfng/J
017893   B6;CBA-Tg(Thy1-GCaMP3)6Gfng/J
014130   B6;CBA-Tg(Thy1-YFP)GJrs/GfngJ
014651   B6;CBA-Tg(Thy1-spH)21Vnmu/J
015814   B6;CBA-Tg(Thy1-spH)64Vnmu/FrkJ
012341   B6;SJL-Tg(Thy1-COP3/EYFP)1Gfng/J
012344   B6;SJL-Tg(Thy1-COP3/EYFP)4Gfng/J
012348   B6;SJL-Tg(Thy1-COP3/EYFP)8Gfng/J
012350   B6;SJL-Tg(Thy1-COP4*H134R/EYFP)20Gfng/J
008004   B6;SJL-Tg(Thy1-ECFP/VAMP2)1Sud/J
012836   B6;SJL-Tg(Thy1-TARDBP)4Singh/J
007610   B6;SJL-Tg(Thy1-cre/ERT2,-EYFP)VGfng/J
012332   B6;SJL-Tg(Thy1-hop/EYFP)2Gfng/J
012334   B6;SJL-Tg(Thy1-hop/EYFP)4Gfng/J
025401   B6SJL-Tg(Thy1-COX8A/Dendra)57Gmnf/J
017590   B6SJL-Tg(Thy1-DCTN1*G59S)M2Pcw/J
007880   B6SJL-Tg(Thy1-Stx1a/EYFP)1Sud/J
007856   B6SJL-Tg(Thy1-Syt1/ECFP)1Sud/J
017589   B6SJL-Tg(Thy1-TARDBP*G298S)S97Pcw/J
017351   BKa.Cg-Ptprcb Bmi1tm1Ilw Thy1a/J
007687   BKa.Cg-Sox17tm1Sjm Ptprcb Thy1a/J
007686   BKa.Cg-Sox17tm2Sjm Ptprcb Thy1a/J
024703   C3A.Cg-Pde6b+Tg(Thy1-CFP)23Jrs/SjJ
007027   C57BL/6-Tg(Thy1-APPSwDutIowa)BWevn/Mmjax
010800   C57BL/6-Tg(Thy1-PTGS2)300Kand/J
010703   C57BL/6-Tg(Thy1-PTGS2)303Kand/J
012769   C57BL/6-Tg(Thy1-Sncg)HvP36Putt/J
024339   C57BL/6J-Tg(Thy1-GCaMP6f)GP5.11Dkim/J
025393   C57BL/6J-Tg(Thy1-GCaMP6f)GP5.17Dkim/J
024276   C57BL/6J-Tg(Thy1-GCaMP6f)GP5.5Dkim/J
025776   C57BL/6J-Tg(Thy1-GCaMP6s)GP4.12Dkim/J
024275   C57BL/6J-Tg(Thy1-GCaMP6s)GP4.3Dkim/J
025533   C57BL/6N-Sncatm1Mjff Tg(Thy1-SNCA)15Mjff/J
016936   C57BL/6N-Tg(Thy1-SNCA)12Mjff/J
017682   C57BL/6N-Tg(Thy1-SNCA)15Mjff/J
005307   CBy.Cg-Thy1a Tg(TcraCl4,TcrbCl4)1Shrm/ShrmJ
005922   CBy.Cg-Thy1a Tg(TcraCl1,TcrbCl1)1Shrm/J
005443   CBy.PL(B6)-Thy1a/ScrJ
024704   D2.Cg-Gpnmb+Tg(Thy1-CFP)23Jrs/SjJ
025018   D2.Cg-Gpnmb+Tg(Thy1-YFP)HJrs/SjJ
018671   D2.Cg-Tg(Thy1-CFP)23Jrs/SjJ
024705   D2.Cg-Tg(Thy1-YFP)HJrs/SjJ
025019   D2.Cg-Tg(Thy1-YFP/Syp)10Jrs/SjJ
008230   FVB(Cg)-Tg(Thy1-SOD1*G93A)T3Hgrd/J
006143   FVB/N-Tg(Thy1-cre)1Vln/J
024476   NOD.Cg-Stat4tm1Gru Thy1a Ifngr1tm1Agt Tg(TcraBDC2.5,TcrbBDC2.5)1Doi/LmbrJ
005686   NOD.Cg-Thy1a Tg(TcraCl4,TcrbCl4)1Shrm/ShrmJ
004483   NOD.NON-Thy1a/1LtJ
002721   NOD.NON-Thy1a/J
005651   SJL.AK-Thy1a/TseJ
003961   SJL.Cg Thy1a-Noxo1hslt/J
021226   STOCK Tg(Thy1-Brainbow3.1)18Jrs/J
021225   STOCK Tg(Thy1-Brainbow3.1)3Jrs/J
021227   STOCK Tg(Thy1-Brainbow3.2)7Jrs/J
013162   STOCK Tg(Thy1-Clomeleon)12Gjau/J
013163   STOCK Tg(Thy1-Clomeleon)13Gjau/J
007788   STOCK Tg(Thy1-EGFP)MJrs/J
012708   STOCK Tg(Thy1-cre/ERT2,-EYFP)HGfng/PyngJ
View Strains carrying other alleles of Thy1     (92 strains)

Additional Web Information

Visit the Alzheimer's Disease Mouse Model Resource site for helpful information on Alzheimer's Disease and research resources.

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Characteristics of this human disease are associated with transgenes and other mutation types in the mouse.
Alzheimer Disease 3
Alzheimer Disease; AD
- Potential model based on transgenic expression of an ortholog of a human gene that is associated with this disease. Phenotypic similarity to the human disease has not been tested.
Acne Inversa, Familial, 3; ACNINV3   (PSEN1)
Cardiomyopathy, Dilated, 1u; CMD1U   (PSEN1)
Frontotemporal Dementia; FTD   (PSEN1)
Pick Disease of Brain   (PSEN1)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Tg(APPSwFlLon,PSEN1*M146L*L286V)6799Vas/0

        involves: C57BL/6 * SJL
  • behavior/neurological phenotype
  • abnormal spatial learning
    • by 4-5 months of age, defects in Y-maze alternation are detected in transgenic mice, indicating impaired spatial learning/memory   (MGI Ref ID J:112949)
  • nervous system phenotype
  • abnormal hippocampus morphology
    • cortical layer 1 is significantly thinner than in control brains   (MGI Ref ID J:112949)
    • abnormal subiculum morphology
      • neurons in subiculum are very pale, or absent   (MGI Ref ID J:112949)
  • abnormal neuron morphology
    • some neurons contain intraneuronal aggregates and display disrupted morphology   (MGI Ref ID J:112949)
    • decreased cerebral cortex pyramidal cell number
      • large neurons in cortical layer 5 are reduced in number   (MGI Ref ID J:112949)
  • amyloid beta deposits
    • mice show Abeta42 deposits at 2 months of age; Abeta40 levels are lower in amyloid deposits; mice show robust intraneuronal amyloid deposition   (MGI Ref ID J:112949)
    • amyloid deposition increases rapidly with increasing age   (MGI Ref ID J:112949)
    • plaques appear first in deep cortical layers and in subiculum, and spread with age to fill most of cortex, subiculum and hippocampus; also, less numerous deposits are observed in thalamus, brainstem and olfactory bulb in older mice   (MGI Ref ID J:112949)
  • brain inflammation
    • transgenic mice display neuroinflammation   (MGI Ref ID J:112949)
  • gliosis
    • microgliosis and astrogliosis is seen in plaque-bearing regions of the brain by 2 months of age; numbers of activated astrocytes and microglia increases with age   (MGI Ref ID J:112949)
  • neurodegeneration
    • synapse degeneration begins at 4 months of age, compared to nontransgenic controls, as shown by reduction in levels of synaptic markers; neurodeneration marker p25 level is ~150% of control at 9 and 12 months   (MGI Ref ID J:112949)
  • immune system phenotype
  • brain inflammation
    • transgenic mice display neuroinflammation   (MGI Ref ID J:112949)
  • homeostasis/metabolism phenotype
  • amyloid beta deposits
    • mice show Abeta42 deposits at 2 months of age; Abeta40 levels are lower in amyloid deposits; mice show robust intraneuronal amyloid deposition   (MGI Ref ID J:112949)
    • amyloid deposition increases rapidly with increasing age   (MGI Ref ID J:112949)
    • plaques appear first in deep cortical layers and in subiculum, and spread with age to fill most of cortex, subiculum and hippocampus; also, less numerous deposits are observed in thalamus, brainstem and olfactory bulb in older mice   (MGI Ref ID J:112949)

Tg(APPSwFlLon,PSEN1*M146L*L286V)6799Vas/?

        involves: C57BL/6 * SJL
  • behavior/neurological phenotype
  • abnormal response to novel object
    • poor novel object recognition   (MGI Ref ID J:208030)
  • impaired contextual conditioning behavior
    • decreased % of time freezing in contextual fear conditioning test   (MGI Ref ID J:208030)
  • impaired cued conditioning behavior
    • decreased % of time freezing in cued fear conditioning test   (MGI Ref ID J:208030)
  • hematopoietic system phenotype
  • abnormal microglial cell activation
    • increased percentage of activated microglia as compared to controls   (MGI Ref ID J:208030)
  • immune system phenotype
  • abnormal microglial cell activation
    • increased percentage of activated microglia as compared to controls   (MGI Ref ID J:208030)
  • nervous system phenotype
  • abnormal astrocyte physiology
    • increased percentage of reactive astrocytes as compared to controls   (MGI Ref ID J:208030)
  • abnormal long term depression
    • decreased magnitude of long term depression as compared to controls   (MGI Ref ID J:208030)
  • abnormal microglial cell activation
    • increased percentage of activated microglia as compared to controls   (MGI Ref ID J:208030)
  • amyloid beta deposits
    • increased plaque load and size as compared to controls   (MGI Ref ID J:208030)
    • high levels of Abeta42 and Abeta40 in the hippocampus as compared to mice that also carry Cdk5r1tm2.1Lht   (MGI Ref ID J:208030)
  • homeostasis/metabolism phenotype
  • amyloid beta deposits
    • increased plaque load and size as compared to controls   (MGI Ref ID J:208030)
    • high levels of Abeta42 and Abeta40 in the hippocampus as compared to mice that also carry Cdk5r1tm2.1Lht   (MGI Ref ID J:208030)

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Tg(APPSwFlLon,PSEN1*M146L*L286V)6799Vas/0

        involves: C57BL/6 * C57BL/6J * CBA * SJL
  • mortality/aging
  • premature death
    • decreased survival at 10 months of age   (MGI Ref ID J:201809)
  • nervous system phenotype
  • amyloid beta deposits   (MGI Ref ID J:201809)
  • homeostasis/metabolism phenotype
  • amyloid beta deposits   (MGI Ref ID J:201809)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Neurobiology Research
Alzheimer's Disease
      APP and PSEN1 mutants
      Presenilin mutants
      strains expressing mutant APP
Neurodegeneration

Research Tools
Neurobiology Research

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Tg(APPSwFlLon,PSEN1*M146L*L286V)6799Vas
Allele Name transgene insertion 6799, Robert Vassar
Allele Type Transgenic (Inserted expressed sequence)
Common Name(s) 5XFAD; 5XFAD APP/PS1; 5XFAD line Tg6799; Tg6799;
Mutation Made By Robert Vassar,   Northwestern University
Strain of Origin(C57BL/6 x SJL)F1
Expressed Gene PSEN1, presenilin 1, human
Expressed Gene APP695, amyloid beta (A4) precursor protein (chimeric), mouse/human chimera
Promoter Thy1, thymus cell antigen 1, theta, mouse, laboratory
General Note Three transgenic lines coexpressing the APP and PSEN1 proteins at high, medium and low levels, respectively designated Tg6799, Tg7031, and Tg7092, were propagated for analysis, most of which employed Tg6799.
Molecular Note Four familial Alzheimer disease- (FAD-) associated mutations were introduced into a single human amyloid precursor protein cDNA: the "Swedish" double mutation (K670N/M671L); the "Florida" mutation (I716V); and the "London" mutation (V717I). Two FAD-associated mutations, M146L and L286V, likewise were introduced into a human presenilin 1 cDNA. Each cDNA was then cloned independently into the mouse thymus cell antigen 1 gene, replacing a segment that contains thymus-specific elements so that expression of the transgenes is targeted only to the brain. Equal molar amounts of the two transgenes were coinjected into pronuclei of single-celled embryos. [MGI Ref ID J:112949] [MGI Ref ID J:32213]
 
 
 

Genotyping

Genotyping Information

Genotyping Protocols

Generic Human PSEN1 cDNA Multiplex1, Melt Curve Analysis
Generic Pde6b, Standard PCR
Generic Tg(APP), Standard PCR
Tg(PSEN1), Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Oakley H; Cole SL; Logan S; Maus E; Shao P; Craft J; Guillozet-Bongaarts A; Ohno M; Disterhoft J; Van Eldik L; Berry R; Vassar R. 2006. Intraneuronal beta-amyloid aggregates, neurodegeneration, and neuron loss in transgenic mice with five familial Alzheimer's disease mutations: potential factors in amyloid plaque formation. J Neurosci 26(40):10129-40. [PubMed: 17021169]  [MGI Ref ID J:112949]

Additional References

Zhao J; Fu Y; Yasvoina M; Shao P; Hitt B; O'Connor T; Logan S; Maus E; Citron M; Berry R; Binder L; Vassar R. 2007. Beta-site amyloid precursor protein cleaving enzyme 1 levels become elevated in neurons around amyloid plaques: implications for Alzheimer's disease pathogenesis. J Neurosci 27(14):3639-49. [PubMed: 17409228]  [MGI Ref ID J:119403]

Tg(APPSwFlLon,PSEN1*M146L*L286V)6799Vas related

Avrahami L; Farfara D; Shaham-Kol M; Vassar R; Frenkel D; Eldar-Finkelman H. 2013. Inhibition of glycogen synthase kinase-3 ameliorates beta-amyloid pathology and restores lysosomal acidification and mammalian target of rapamycin activity in the Alzheimer disease mouse model: in vivo and in vitro studies. J Biol Chem 288(2):1295-306. [PubMed: 23155049]  [MGI Ref ID J:193739]

Aytan N; Choi JK; Carreras I; Kowall NW; Jenkins BG; Dedeoglu A. 2013. Combination therapy in a transgenic model of Alzheimer's disease. Exp Neurol 250:228-38. [PubMed: 24120437]  [MGI Ref ID J:206610]

Baik SH; Cha MY; Hyun YM; Cho H; Hamza B; Kim DK; Han SH; Choi H; Kim KH; Moon M; Lee J; Kim M; Irimia D; Mook-Jung I. 2014. Migration of neutrophils targeting amyloid plaques in Alzheimer's disease mouse model. Neurobiol Aging 35(6):1286-92. [PubMed: 24485508]  [MGI Ref ID J:213882]

Brower CS; Piatkov KI; Varshavsky A. 2013. Neurodegeneration-associated protein fragments as short-lived substrates of the N-end rule pathway. Mol Cell 50(2):161-71. [PubMed: 23499006]  [MGI Ref ID J:198127]

Burgert T; Schmidt V; Caglayan S; Lin F; Fuchtbauer A; Fuchtbauer EM; Nykjaer A; Carlo AS; Willnow TE. 2013. SORLA-dependent and -independent functions for PACS1 in control of amyloidogenic processes. Mol Cell Biol 33(21):4308-20. [PubMed: 24001769]  [MGI Ref ID J:205418]

Buskila Y; Crowe SE; Ellis-Davies GC. 2013. Synaptic deficits in layer 5 neurons precede overt structural decay in 5xFAD mice. Neuroscience 254:152-9. [PubMed: 24055684]  [MGI Ref ID J:207428]

Cai Y; Xue ZQ; Zhang XM; Li MB; Wang H; Luo XG; Cai H; Yan XX. 2012. An age-related axon terminal pathology around the first olfactory relay that involves amyloidogenic protein overexpression without plaque formation. Neuroscience 215:160-73. [PubMed: 22542680]  [MGI Ref ID J:192436]

Carlo AS; Gustafsen C; Mastrobuoni G; Nielsen MS; Burgert T; Hartl D; Rohe M; Nykjaer A; Herz J; Heeren J; Kempa S; Petersen CM; Willnow TE. 2013. The pro-neurotrophin receptor sortilin is a major neuronal apolipoprotein E receptor for catabolism of amyloid-beta peptide in the brain. J Neurosci 33(1):358-70. [PubMed: 23283348]  [MGI Ref ID J:193915]

Chen R; Zhang J; Wu Y; Wang D; Feng G; Tang YP; Teng Z; Chen C. 2012. Monoacylglycerol lipase is a therapeutic target for Alzheimer's disease. Cell Rep 2(5):1329-39. [PubMed: 23122958]  [MGI Ref ID J:196345]

Christensen DZ; Bayer TA; Wirths O. 2009. Formic acid is essential for immunohistochemical detection of aggregated intraneuronal Abeta peptides in mouse models of Alzheimer's disease. Brain Res 1301:116-25. [PubMed: 19751708]  [MGI Ref ID J:158791]

Cortes-Canteli M; Paul J; Norris EH; Bronstein R; Ahn HJ; Zamolodchikov D; Bhuvanendran S; Fenz KM; Strickland S. 2010. Fibrinogen and beta-amyloid association alters thrombosis and fibrinolysis: a possible contributing factor to Alzheimer's disease. Neuron 66(5):695-709. [PubMed: 20547128]  [MGI Ref ID J:167873]

Crouzin N; Baranger K; Cavalier M; Marchalant Y; Cohen-Solal C; Roman FS; Khrestchatisky M; Rivera S; Feron F; Vignes M. 2013. Area-specific alterations of synaptic plasticity in the 5XFAD mouse model of Alzheimer's disease: dissociation between somatosensory cortex and hippocampus. PLoS One 8(9):e74667. [PubMed: 24069328]  [MGI Ref ID J:206029]

Crowe SE; Ellis-Davies GC. 2013. In vivo characterization of a bigenic fluorescent mouse model of Alzheimer's disease with neurodegeneration. J Comp Neurol 521(10):Spc1. [PubMed: 23605442]  [MGI Ref ID J:200729]

Cui Y; Huang M; He Y; Zhang S; Luo Y. 2011. Genetic Ablation of Apolipoprotein A-IV Accelerates Alzheimer's Disease Pathogenesis in a Mouse Model. Am J Pathol 178(3):1298-308. [PubMed: 21356380]  [MGI Ref ID J:169682]

Devi L; Ohno M. 2013. Deletion of the eIF2alpha Kinase GCN2 fails to rescue the memory decline associated with Alzheimer's disease. PLoS One 8(10):e77335. [PubMed: 24146979]  [MGI Ref ID J:209106]

Devi L; Ohno M. 2010. Genetic reductions of beta-site amyloid precursor protein-cleaving enzyme 1 and amyloid-beta ameliorate impairment of conditioned taste aversion memory in 5XFAD Alzheimer's disease model mice. Eur J Neurosci 31(1):110-8. [PubMed: 20092558]  [MGI Ref ID J:158363]

Devi L; Ohno M. 2012. Mitochondrial dysfunction and accumulation of the beta-secretase-cleaved C-terminal fragment of APP in Alzheimer's disease transgenic mice. Neurobiol Dis 45(1):417-24. [PubMed: 21933711]  [MGI Ref ID J:179837]

Devi L; Ohno M. 2010. Phospho-eIF2alpha level is important for determining abilities of BACE1 reduction to rescue cholinergic neurodegeneration and memory defects in 5XFAD mice. PLoS One 5(9):e12974. [PubMed: 20886088]  [MGI Ref ID J:165103]

Frost JL; Le KX; Cynis H; Ekpo E; Kleinschmidt M; Palmour RM; Ervin FR; Snigdha S; Cotman CW; Saido TC; Vassar RJ; George-Hyslop PS; Ikezu T; Schilling S; Demuth HU; Lemere CA. 2013. Pyroglutamate-3 Amyloid-beta Deposition in the Brains of Humans, Non-Human Primates, Canines, and Alzheimer Disease-Like Transgenic Mouse Models. Am J Pathol 183(2):369-81. [PubMed: 23747948]  [MGI Ref ID J:199082]

Graff J; Rei D; Guan JS; Wang WY; Seo J; Hennig KM; Nieland TJ; Fass DM; Kao PF; Kahn M; Su SC; Samiei A; Joseph N; Haggarty SJ; Delalle I; Tsai LH. 2012. An epigenetic blockade of cognitive functions in the neurodegenerating brain. Nature 483(7388):222-6. [PubMed: 22388814]  [MGI Ref ID J:181683]

Guo Z; Zhang L; Wu Z; Chen Y; Wang F; Chen G. 2014. In Vivo direct reprogramming of reactive glial cells into functional neurons after brain injury and in an Alzheimer's disease model. Cell Stem Cell 14(2):188-202. [PubMed: 24360883]  [MGI Ref ID J:210106]

Heraud C; Goufak D; Ando K; Leroy K; Suain V; Yilmaz Z; De Decker R; Authelet M; Laporte V; Octave JN; Brion JP. 2013. Increased misfolding and truncation of tau in APP/PS1/tau transgenic mice compared to mutant tau mice. Neurobiol Dis 62C:100-112. [PubMed: 24076100]  [MGI Ref ID J:201809]

Hillmann A; Hahn S; Schilling S; Hoffmann T; Demuth HU; Bulic B; Schneider-Axmann T; Bayer TA; Weggen S; Wirths O. 2012. No improvement after chronic ibuprofen treatment in the 5XFAD mouse model of Alzheimer's disease. Neurobiol Aging 33(4):833.e39-50. [PubMed: 21943956]  [MGI Ref ID J:188189]

Jawhar S; Trawicka A; Jenneckens C; Bayer TA; Wirths O. 2012. Motor deficits, neuron loss, and reduced anxiety coinciding with axonal degeneration and intraneuronal Abeta aggregation in the 5XFAD mouse model of Alzheimer's disease. Neurobiol Aging 33(1):196.e29-40. [PubMed: 20619937]  [MGI Ref ID J:188235]

Jawhar S; Wirths O; Schilling S; Graubner S; Demuth HU; Bayer TA. 2011. Overexpression of glutaminyl cyclase, the enzyme responsible for pyroglutamate A{beta} formation, induces behavioral deficits, and glutaminyl cyclase knock-out rescues the behavioral phenotype in 5XFAD mice. J Biol Chem 286(6):4454-60. [PubMed: 21148560]  [MGI Ref ID J:169481]

Kalinin S; Polak PE; Lin SX; Sakharkar AJ; Pandey SC; Feinstein DL. 2012. The noradrenaline precursor L-DOPS reduces pathology in a mouse model of Alzheimer's disease. Neurobiol Aging 33(8):1651-63. [PubMed: 21705113]  [MGI Ref ID J:188202]

Katsouri L; Georgopoulos S. 2011. Lack of LDL receptor enhances amyloid deposition and decreases glial response in an Alzheimer's disease mouse model. PLoS One 6(7):e21880. [PubMed: 21755005]  [MGI Ref ID J:174945]

Kim C; Nam DW; Park SY; Song H; Hong HS; Boo JH; Jung ES; Kim Y; Baek JY; Kim KS; Cho JW; Mook-Jung I. 2013. O-linked beta-N-acetylglucosaminidase inhibitor attenuates beta-amyloid plaque and rescues memory impairment. Neurobiol Aging 34(1):275-85. [PubMed: 22503002]  [MGI Ref ID J:191369]

Kimura R; Devi L; Ohno M. 2010. Partial reduction of BACE1 improves synaptic plasticity, recent and remote memories in Alzheimer's disease transgenic mice. J Neurochem 113(1):248-61. [PubMed: 20089133]  [MGI Ref ID J:174832]

Kimura R; Ohno M. 2009. Impairments in remote memory stabilization precede hippocampal synaptic and cognitive failures in 5XFAD Alzheimer mouse model. Neurobiol Dis 33(2):229-35. [PubMed: 19026746]  [MGI Ref ID J:144743]

Kook SY; Hong HS; Moon M; Ha CM; Chang S; Mook-Jung I. 2012. Abeta1-42-RAGE Interaction Disrupts Tight Junctions of the Blood-Brain Barrier Via Ca2+-Calcineurin Signaling. J Neurosci 32(26):8845-54. [PubMed: 22745485]  [MGI Ref ID J:185647]

Lee JE; Han PL. 2013. An update of animal models of Alzheimer disease with a reevaluation of plaque depositions. Exp Neurobiol 22(2):84-95. [PubMed: 23833557]  [MGI Ref ID J:202532]

Leroy K; Ando K; Laporte V; Dedecker R; Suain V; Authelet M; Heraud C; Pierrot N; Yilmaz Z; Octave JN; Brion JP. 2012. Lack of tau proteins rescues neuronal cell death and decreases amyloidogenic processing of APP in APP/PS1 mice. Am J Pathol 181(6):1928-40. [PubMed: 23026200]  [MGI Ref ID J:190305]

Mijatovic J; Piltonen M; Alberton P; Mannisto PT; Saarma M; Piepponen TP. 2011. Constitutive Ret signaling is protective for dopaminergic cell bodies but not for axonal terminals. Neurobiol Aging 32(8):1486-94. [PubMed: 19767128]  [MGI Ref ID J:176716]

Moechars D; Lorent K; De Strooper B; Dewachter I; Van Leuven F. 1996. Expression in brain of amyloid precursor protein mutated in the alpha-secretase site causes disturbed behavior, neuronal degeneration and premature death in transgenic mice. EMBO J 15(6):1265-74. [PubMed: 8635459]  [MGI Ref ID J:32213]

Noh H; Seo H. 2014. Age-dependent effects of valproic acid in Alzheimer's disease (AD) mice are associated with nerve growth factor (NGF) regulation. Neuroscience 266:255-65. [PubMed: 24568732]  [MGI Ref ID J:210335]

Ohno M. 2009. Failures to reconsolidate memory in a mouse model of Alzheimer's disease. Neurobiol Learn Mem 92(3):455-9. [PubMed: 19435612]  [MGI Ref ID J:154421]

Ohno M; Cole SL; Yasvoina M; Zhao J; Citron M; Berry R; Disterhoft JF; Vassar R. 2007. BACE1 gene deletion prevents neuron loss and memory deficits in 5XFAD APP/PS1 transgenic mice. Neurobiol Dis 26(1):134-45. [PubMed: 17258906]  [MGI Ref ID J:119002]

Pietri M; Dakowski C; Hannaoui S; Alleaume-Butaux A; Hernandez-Rapp J; Ragagnin A; Mouillet-Richard S; Haik S; Bailly Y; Peyrin JM; Launay JM; Kellermann O; Schneider B. 2013. PDK1 decreases TACE-mediated alpha-secretase activity and promotes disease progression in prion and Alzheimer's diseases. Nat Med 19(9):1124-31. [PubMed: 23955714]  [MGI Ref ID J:202038]

Rojas S; Herance JR; Gispert JD; Abad S; Torrent E; Jimenez X; Pareto D; Perpina U; Sarroca S; Rodriguez E; Ortega-Aznar A; Sanfeliu C. 2013. In vivo evaluation of amyloid deposition and brain glucose metabolism of 5XFAD mice using positron emission tomography. Neurobiol Aging 34(7):1790-8. [PubMed: 23402900]  [MGI Ref ID J:211692]

Sadleir KR; Vassar R. 2012. Cdk5 protein inhibition and Abeta42 increase BACE1 protein level in primary neurons by a post-transcriptional mechanism: implications of CDK5 as a therapeutic target for Alzheimer disease. J Biol Chem 287(10):7224-35. [PubMed: 22223639]  [MGI Ref ID J:182773]

Seo J; Giusti-Rodriguez P; Zhou Y; Rudenko A; Cho S; Ota KT; Park C; Patzke H; Madabhushi R; Pan L; Mungenast AE; Guan JS; Delalle I; Tsai LH. 2014. Activity-Dependent p25 Generation Regulates Synaptic Plasticity and Abeta-Induced Cognitive Impairment. Cell 157(2):486-98. [PubMed: 24725413]  [MGI Ref ID J:208030]

Sesele K; Thanopoulou K; Paouri E; Tsefou E; Klinakis A; Georgopoulos S. 2013. Conditional inactivation of nicastrin restricts amyloid deposition in an Alzheimer's disease mouse model. Aging Cell 12(6):1032-40. [PubMed: 23826707]  [MGI Ref ID J:210209]

Torres M; Price SL; Fiol-Deroque MA; Marcilla-Etxenike A; Ahyayauch H; Barcelo-Coblijn G; Teres S; Katsouri L; Ordinas M; Lopez DJ; Ibarguren M; Goni FM; Busquets X; Vitorica J; Sastre M; Escriba PV. 2014. Membrane lipid modifications and therapeutic effects mediated by hydroxydocosahexaenoic acid on Alzheimer's disease. Biochim Biophys Acta 1838(6):1680-92. [PubMed: 24374316]  [MGI Ref ID J:210860]

Wirths O; Erck C; Martens H; Harmeier A; Geumann C; Jawhar S; Kumar S; Multhaup G; Walter J; Ingelsson M; Degerman-Gunnarsson M; Kalimo H; Huitinga I; Lannfelt L; Bayer TA. 2010. Identification of low molecular weight pyroglutamate A{beta} oligomers in Alzheimer disease: a novel tool for therapy and diagnosis. J Biol Chem 285(53):41517-24. [PubMed: 20971852]  [MGI Ref ID J:167567]

Wittnam JL; Portelius E; Zetterberg H; Gustavsson MK; Schilling S; Koch B; Demuth HU; Blennow K; Wirths O; Bayer TA. 2012. Pyroglutamate amyloid beta (Abeta) aggravates behavioral deficits in transgenic amyloid mouse model for Alzheimer disease. J Biol Chem 287(11):8154-62. [PubMed: 22267726]  [MGI Ref ID J:182761]

Woo DC; Lee SH; Lee DW; Kim SY; Kim GY; Rhim HS; Choi CB; Kim HY; Lee CU; Choe BY. 2010. Regional metabolic alteration of Alzheimer's disease in mouse brain expressing mutant human APP-PS1 by 1H HR-MAS. Behav Brain Res 211(1):125-31. [PubMed: 20307581]  [MGI Ref ID J:159678]

You H; Tsutsui S; Hameed S; Kannanayakal TJ; Chen L; Xia P; Engbers JD; Lipton SA; Stys PK; Zamponi GW. 2012. Abeta neurotoxicity depends on interactions between copper ions, prion protein, and N-methyl-D-aspartate receptors. Proc Natl Acad Sci U S A 109(5):1737-42. [PubMed: 22307640]  [MGI Ref ID J:182028]

Youmans KL; Tai LM; Nwabuisi-Heath E; Jungbauer L; Kanekiyo T; Gan M; Kim J; Eimer WA; Estus S; Rebeck GW; Weeber EJ; Bu G; Yu C; Ladu MJ. 2012. APOE4-specific changes in Abeta accumulation in a new transgenic mouse model of Alzheimer disease. J Biol Chem 287(50):41774-86. [PubMed: 23060451]  [MGI Ref ID J:193422]

Yun SM; Cho SJ; Song JC; Song SY; Jo SA; Jo C; Yoon K; Tanzi RE; Choi EJ; Koh YH. 2013. SUMO1 modulates Abeta generation via BACE1 accumulation. Neurobiol Aging 34(3):650-62. [PubMed: 22975420]  [MGI Ref ID J:194511]

Zhang XM; Cai Y; Xiong K; Cai H; Luo XG; Feng JC; Clough RW; Struble RG; Patrylo PR; Yan XX. 2009. Beta-secretase-1 elevation in transgenic mouse models of Alzheimer's disease is associated with synaptic/axonal pathology and amyloidogenesis: implications for neuritic plaque development. Eur J Neurosci 30(12):2271-83. [PubMed: 20092570]  [MGI Ref ID J:157228]

Zhao H; Chang R; Che H; Wang J; Yang L; Fang W; Xia Y; Li N; Ma Q; Wang X. 2013. Hyperphosphorylation of tau protein by calpain regulation in retina of Alzheimer's disease transgenic mouse. Neurosci Lett 551:12-6. [PubMed: 23810804]  [MGI Ref ID J:201685]

Zhao J; Fu Y; Yasvoina M; Shao P; Hitt B; O'Connor T; Logan S; Maus E; Citron M; Berry R; Binder L; Vassar R. 2007. Beta-site amyloid precursor protein cleaving enzyme 1 levels become elevated in neurons around amyloid plaques: implications for Alzheimer's disease pathogenesis. J Neurosci 27(14):3639-49. [PubMed: 17409228]  [MGI Ref ID J:119403]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX10

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, hemizygous mice may be bred to B6SJLF1/J (Stock No. 100012).
Mating SystemHemizygote x F1         (Female x Male)   28-OCT-13
F1 x Hemizygote         (Female x Male)   28-OCT-13
Diet Information LabDiet® 5K52/5K67

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See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Terms of Use


General Terms and Conditions


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