Strain Name: |
B6.Cg-H2bm1 Tg(GUSB)4Sly/SndsJ |
|---|---|
Stock Number: |
006558 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Genes & Alleles | GUSB; Gusb; H2-K; H2-Kbm1; H2; Tg(GUSB)4Sly; ; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Chemically Induced Mutation Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Congenic Type JAX® GEMM® Strain - Major Histocompatibility Congenic Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Species laboratory mouse Donating Investigator Brian Soper, The Jackson Laboratory Generation F15pN1 Strain Description
Mice that are homozygous for this transgene and the Gusbmps allele have approximately 20-fold higher beta-glucuronidase enzyme activity than wildtype controls. Distribution of human enzyme activity throughout various tissues mimics the endogenous mouse enzyme activity pattern. Of note, mice homozygous for both the transgene and the Gusbmps allele do not exhibit an accumulation of undegraded glycosaminoglycans. These transgenic mice do not carry the Gusbmps allele. Mice that are hemizygous for this transgene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. This mutant mouse strain may be useful in studies of lysomal storage diseases and mucopolysaccharidosis VII (Sly syndrome).Strain Development
The entire human beta-glucuronidase gene with 1.6kb of 5'-flanking sequence and 3.8kb of 3'-flanking sequence was microinjected into C57BL/6J zygotes. Male mice from founder line 4 that were hemizygous for the transgenic insert were crossed with female mice homozygous for the Gusbmps allele. Mice homozygous for both the transgene and the Gusbmps allele were produced by intercross. These mice were maintained by crossing to B6.C-H2bm1/ByJ (Stock No. 001060) and no longer carry the Gusbmps allele.The Donating Investigator maintained the colony by breeding mice homozygous for the transgene.
Gene & Allele Details
| Allele Symbol | Tg(GUSB)4Sly | ||
|---|---|---|---|
| Allele Name | transgene insertion 4, William S Sly | ||
| Common Name(s) | TgGUSB; | ||
| Strain of Origin | C57BL/6J | ||
| Expressed Gene | Gusb, glucuronidase, beta, mouse, laboratory | ||
| Promoter | GUSB, glucuronidase, beta, human | ||
| Molecular Note | The entire human beta-glucuronidase gene with 1.6kb of 5'-flanking sequence and 3.8kb of 3'-flanking sequence was microinjected into C57BL/6J zygotes. Founder line 4 was subsequently propagated. Mice that are homozygous for this transgene and the Gusbmps allele have approximately 20-fold higher beta-glucuronidase enzyme activity than wildtype controls. Distribution of human enzyme activity throughout various tissues mimics the endogenous mouse enzyme activity pattern. [MGI Ref ID J:21256] | ||
| Gene Symbol and Name | H2, histocompatibility-2, MHC | ||
| Chromosome | 17 | ||
| Gene Common Name(s) | H-2; MHC-II; | ||
| Allele Symbol | H2-Kbm1 | ||
| Allele Name | b haplotype mutation 1 | ||
| Common Name(s) | H(z1); H-2ba; H-2bm1; Kbm1; bm1; | ||
| Gene Symbol and Name | H2-K, histocompatibility 2, K region | ||
| Chromosome | 17 | ||
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, these mice can be bred as hemizygotes. The Donating Investigator maintained the colony by breeding mice homozygous for the transgene. |
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Related Strains
Strains carrying H2-Kbm1 allele
000368 B6.C-H2bm1/By 006559 B6.C-H2bm1/ByBir-Gusbmps/BrkJ 000256 B6.C-H2bm1/ByBir-Gusbmps/J 001060 B6.C-H2bm1/ByJ 002432 B6J x B6.C-H2bm1/ByJ-Cdh23v-J/J View Strains carrying H2-Kbm1 (5 strains)
001880 STOCK Gusbmps Tg(GUSB)4Sly/BirJ
Additional Web Information
Congenic Nomenclature
Research Applications
This mouse can be used to support research in many areas including:
Metabolism Research
Research Tools
Metabolism Research
References
Selected Reference(s)
Additional ReferencesKyle JW; Birkenmeier EH; Gwynn B; Vogler C; Hoppe PC; Hoffmann JW; Sly WS. 1990. Correction of murine mucopolysaccharidosis VII by a human beta-glucuronidase transgene. Proc Natl Acad Sci U S A 87(10):3914-8. [PubMed: 2111021] [MGI Ref ID J:21256]
Price and Supply Information
| Strain Name: | B6.Cg-H2bm1 Tg(GUSB)4Sly/SndsJ |
| Stock Number: | 006558 |
Price Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below |
| Control Information | View Control Information in Strain Details. |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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