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| GFP labels all cells in this strain, with the exception of erythrocytes and hair. | |||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
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Description
Strain Information
Former Names C57BL/6-Tg(ACTB-EGFP)131Osb/LeySopJ (Changed: 08-MAY-08 ) B6.Cg-Tg(ACTB-EGFP)1Osb/LeySopJ (Changed: 17-OCT-07 ) C57BL/6-Tg(ACTB-EGFP)1Osb/LeySopJ (Changed: 03-MAY-07 ) C57BL/6-Tg(CAGG-EGFP)1Ley/J (Changed: 03-MAY-07 ) Type Coisogenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System +/+ sibling x Hemizygote (Female x Male) 02-MAY-08 Species laboratory mouse Generation ?+NE4F2 (17-MAY-13)
Generation DefinitionsDonating Investigator Brian Soper, The Jackson Laboratory Description
This transgenic mouse line with an "enhanced" GFP (EGFP) cDNA under the control of a chicken beta-actin promoter and cytomegalovirus enhancer makes all of the tissues, with the exception of erythrocytes and hair, appear green under excitation light. The viability of homozygous mice is unknown.Of note, it has been the experience at The Jackson Laboratory that Stock No. 006567 (C57BL/6-Tg(CAG-EGFP)131Osb/LeySopJ) demonstrates the highest proportion of GFP expressing cell populations in bone marrow, thymus, spleen and peripheral blood when compared to Stock No. 003291 (C57BL/6-Tg(CAG-EGFP)1Osb/J) and Stock No. 007075 (CByJ.B6-Tg(CAG-EGFP)1Osb/J).
View the pdf document on GFP Expressing Lymphocyte Populations in C57BL/6-Tg(CAG-EGFP)1Osb/J, C57BL/6-Tg(CAG-EGFP)131Osb/LeySopJ and CByJ.B6-Tg(CAG-EGFP)1Osb/J.
Development
The EcoRI sites included in the PCR primers were used to introduce the amplified EGFP cDNA into a pCAGGS expression vector containing the chicken beta-actin promoter and cytomegalovirus enhancer, beta-actin intron and bovine globin poly-adenylation signal. The entire insert with the promoter and coding sequence was excised with Bam-HI and SalI and gel-purified. The transgenic construct was was introduced into C57BL/6 donor eggs. Founder mice were bred to C57BL/6 mice, and backcrossed to the same for 11 generations.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (357 strains)
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
GFP relatedResearch Tools
Fluorescent Proteins
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(CAG-EGFP)131Osb | ||
|---|---|---|---|
| Allele Name | transgene insertion 131, Research Institute for Microbial Diseases, Osaka University | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | Osb-Y01; Tg(ACTB-EGFP)131Osb; Tg(ACTB-EGFP)Y01Osb; Tg(Act-EGFP)C14-Y01-FM131Osb; Tg(CAG-EGFP)C14-Y01-FM131Osb; Tg(act-EGFP)Y01Osb; Tg14(act-EGFP)ObsY01; TgN(act-EGFP)ObsC14-Yo1-FM1310; Y01; | ||
| Strain of Origin | C57BL/6 | ||
| Site of Expression | All of the tissues, with the exception of erythrocytes and hair, appear green under excitation light. | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Promoter | ACTB, actin, beta, chicken | ||
| General Note | This transgene was made using the same construct as Tg(ACTB-EGFP)1Osb, but in this case it was injected into C57BL/6 fertilized oocytes. According to the Center for Animal Resources and Development Database (CARD R-Base), which distributes it, the transgene integrated on Chr 14D1. | ||
| Molecular Note | The transgene comprises the cDNA encoding enhanced green fluorescent protein (EGFP), from the jellyfish Aequoria victoria, downstream of the "CAG promoter" -- which consists of the cytomegalovirus immediate early (CMV-EI) enhancer followed by a 1.3-kb DNA segment including the promoter, first exon and first intron of the chicken beta-actin gene, with the 3' splice junction sequence replaced by that of the rabbit hemoglobin beta gene -- and followed by the rabbit beta-hemoglobin polyadenylation signal and 3' flanking sequence. [MGI Ref ID J:87415] | ||
| Gene Symbol and Name | Tg(CAG-EGFP)131Osb, transgene insertion 131, Research Institute for Microbial Diseases, Osaka University | ||
| Chromosome | 14 | ||
| Gene Common Name(s) | Tg(ACTB-EGFP)131Osb; Tg(ACTB-EGFP)Y01Osb; Tg(Act-EGFP)C14-Y01-FM131Osb; Tg(CAG-EGFP)C14-Y01-FM131Osb; Tg(act-EGFP)Y01Osb; Tg14(act-EGFP)ObsY01; TgN(act-EGFP)ObsC14-Yo1-FM1310; Y01; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Fluorescent Proteins (Generic GFP), Melt Curve Analysis
Fluorescent Proteins (Generic GFP), Standard PCR
Fluorescent Proteins -- Generic GFP, QPCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Okabe M; Ikawa M; Kominami K; Nakanishi T; Nishimune Y. 1997. 'Green mice' as a source of ubiquitous green cells. FEBS Lett 407(3):313-9. [PubMed: 9175875] [MGI Ref ID J:87415]
Additional References
Tg(CAG-EGFP)131Osb relatedBadeanlou L; Furlan-Freguia C; Yang G; Ruf W; Samad F. 2011. Tissue factor-protease-activated receptor 2 signaling promotes diet-induced obesity and adipose inflammation. Nat Med 17(11):1490-7. [PubMed: 22019885] [MGI Ref ID J:178106]
Chretien F; Dreyfus PA; Christov C; Caramelle P; Lagrange JL; Chazaud B; Gherardi RK. 2005. In vivo fusion of circulating fluorescent cells with dystrophin-deficient myofibers results in extensive sarcoplasmic fluorescence expression but limited dystrophin sarcolemmal expression. Am J Pathol 166(6):1741-8. [PubMed: 15920159] [MGI Ref ID J:98788]
Davies PS; Powell AE; Swain JR; Wong MH. 2009. Inflammation and proliferation act together to mediate intestinal cell fusion. PLoS One 4(8):e6530. [PubMed: 19657387] [MGI Ref ID J:152479]
Einstein O; Ben-Menachem-Tzidon O; Mizrachi-Kol R; Reinhartz E; Grigoriadis N; Ben-Hur T. 2006. Survival of neural precursor cells in growth factor-poor environment: implications for transplantation in chronic disease. Glia 53(4):449-55. [PubMed: 16345032] [MGI Ref ID J:156140]
Engstrom L; Ruud J; Eskilsson A; Larsson A; Mackerlova L; Kugelberg U; Qian H; Vasilache AM; Larsson P; Engblom D; Sigvardsson M; Jonsson JI; Blomqvist A. 2012. Lipopolysaccharide-induced fever depends on prostaglandin E2 production specifically in brain endothelial cells. Endocrinology 153(10):4849-61. [PubMed: 22872578] [MGI Ref ID J:191440]
Goldman DC; Bailey AS; Pfaffle DL; Al Masri A; Christian JL; Fleming WH. 2009. BMP4 regulates the hematopoietic stem cell niche. Blood 114(20):4393-401. [PubMed: 19759357] [MGI Ref ID J:154923]
Hara K; Kanai-Azuma M; Uemura M; Shitara H; Taya C; Yonekawa H; Kawakami H; Tsunekawa N; Kurohmaru M; Kanai Y. 2009. Evidence for crucial role of hindgut expansion in directing proper migration of primordial germ cells in mouse early embryogenesis. Dev Biol 330(2):427-39. [PubMed: 19371732] [MGI Ref ID J:149220]
Hasegawa Y; Ogihara T; Yamada T; Ishigaki Y; Imai J; Uno K; Gao J; Kaneko K; Ishihara H; Sasano H; Nakauchi H; Oka Y; Katagiri H. 2007. Bone marrow (BM) transplantation promotes beta-cell regeneration after acute injury through BM cell mobilization. Endocrinology 148(5):2006-15. [PubMed: 17255204] [MGI Ref ID J:129611]
Hofbauer JP; Heyder C; Denk U; Kocher T; Holler C; Trapin D; Asslaber D; Tinhofer I; Greil R; Egle A. 2011. Development of CLL in the TCL1 transgenic mouse model is associated with severe skewing of the T-cell compartment homologous to human CLL. Leukemia 25(9):1452-8. [PubMed: 21606964] [MGI Ref ID J:175729]
Ieronimakis N; Hays A; Reyes M. 2012. Bone marrow-derived cells do not engraft into skeletal muscle microvasculature but promote angiogenesis after acute injury. Exp Hematol 40(3):238-249.e3. [PubMed: 22155292] [MGI Ref ID J:191531]
Kanatsu-Shinohara M; Morimoto T; Toyokuni S; Shinohara T. 2004. Regulation of mouse spermatogonial stem cell self-renewing division by the pituitary gland. Biol Reprod 70(6):1731-7. [PubMed: 14766726] [MGI Ref ID J:132826]
Lau AW; Biester S; Cornall RJ; Forrester JV. 2008. Lipopolysaccharide-Activated IL-10-Secreting Dendritic Cells Suppress Experimental Autoimmune Uveoretinitis by MHCII-Dependent Activation of CD62L-Expressing Regulatory T Cells. J Immunol 180(6):3889-99. [PubMed: 18322197] [MGI Ref ID J:133030]
Murai M; Yoneyama H; Ezaki T; Suematsu M; Terashima Y; Harada A; Hamada H; Asakura H; Ishikawa H; Matsushima K. 2003. Peyer's patch is the essential site in initiating murine acute and lethal graft-versus-host reaction. Nat Immunol 4(2):154-60. [PubMed: 12524535] [MGI Ref ID J:94937]
Muramatsu M; Yamamoto S; Osawa T; Shibuya M. 2010. Vascular endothelial growth factor receptor-1 signaling promotes mobilization of macrophage lineage cells from bone marrow and stimulates solid tumor growth. Cancer Res 70(20):8211-21. [PubMed: 20924106] [MGI Ref ID J:165563]
Nakanishi T; Kuroiwa A; Yamada S; Isotani A; Yamashita A; Tairaka A; Hayashi T; Takagi T; Ikawa M; Matsuda Y; Okabe M. 2002. FISH analysis of 142 EGFP transgene integration sites into the mouse genome. Genomics 80(6):564-74. [PubMed: 12504848] [MGI Ref ID J:110379]
Ohtsuka M; Ogiwara S; Miura H; Mizutani A; Warita T; Sato M; Imai K; Hozumi K; Sato T; Tanaka M; Kimura M; Inoko H. 2010. Pronuclear injection-based mouse targeted transgenesis for reproducible and highly efficient transgene expression. Nucleic Acids Res 38(22):e198. [PubMed: 20880997] [MGI Ref ID J:173808]
Powell AE; Anderson EC; Davies PS; Silk AD; Pelz C; Impey S; Wong MH. 2011. Fusion between Intestinal Epithelial Cells and Macrophages in a Cancer Context Results in Nuclear Reprogramming. Cancer Res 71(4):1497-505. [PubMed: 21303980] [MGI Ref ID J:169371]
Rock JR; Onaitis MW; Rawlins EL; Lu Y; Clark CP; Xue Y; Randell SH; Hogan BL. 2009. Basal cells as stem cells of the mouse trachea and human airway epithelium. Proc Natl Acad Sci U S A 106(31):12771-5. [PubMed: 19625615] [MGI Ref ID J:152012]
Rosin NL; Falkenham A; Sopel MJ; Lee TD; Legare JF. 2013. Regulation and Role of Connective Tissue Growth Factor in AngII-Induced Myocardial Fibrosis. Am J Pathol 182(3):714-26. [PubMed: 23287510] [MGI Ref ID J:193265]
Serafini M; Dylla SJ; Oki M; Heremans Y; Tolar J; Jiang Y; Buckley SM; Pelacho B; Burns TC; Frommer S; Rossi DJ; Bryder D; Panoskaltsis-Mortari A; O'Shaughnessy MJ; Nelson-Holte M; Fine GC; Weissman IL; Blazar BR; Verfaillie CM. 2007. Hematopoietic reconstitution by multipotent adult progenitor cells: precursors to long-term hematopoietic stem cells. J Exp Med 204(1):129-39. [PubMed: 17227908] [MGI Ref ID J:125294]
Sumoza-Toledo A; Lange I; Cortado H; Bhagat H; Mori Y; Fleig A; Penner R; Partida-Sanchez S. 2011. Dendritic cell maturation and chemotaxis is regulated by TRPM2-mediated lysosomal Ca2+ release. FASEB J 25(10):3529-42. [PubMed: 21753080] [MGI Ref ID J:178425]
Tanaka DH; Oiwa R; Sasaki E; Nakajima K. 2011. Changes in cortical interneuron migration contribute to the evolution of the neocortex. Proc Natl Acad Sci U S A 108(19):8015-20. [PubMed: 21518872] [MGI Ref ID J:172193]
Taoufik E; Valable S; Muller GJ; Roberts ML; Divoux D; Tinel A; Voulgari-Kokota A; Tseveleki V; Altruda F; Lassmann H; Petit E; Probert L. 2007. FLIP(L) protects neurons against in vivo ischemia and in vitro glucose deprivation-induced cell death. J Neurosci 27(25):6633-46. [PubMed: 17581950] [MGI Ref ID J:122799]
Uemura M; Hara K; Shitara H; Ishii R; Tsunekawa N; Miura Y; Kurohmaru M; Taya C; Yonekawa H; Kanai-Azuma M; Kanai Y. 2010. Expression and function of mouse Sox17 gene in the specification of gallbladder/bile-duct progenitors during early foregut morphogenesis. Biochem Biophys Res Commun 391(1):357-63. [PubMed: 19913509] [MGI Ref ID J:156731]
Uemura M; Nakao S; Suzuki ST; Takeichi M; Hirano S. 2007. OL-Protocadherin is essential for growth of striatal axons and thalamocortical projections. Nat Neurosci 10(9):1151-9. [PubMed: 17721516] [MGI Ref ID J:127418]
Weimann JM; Johansson CB; Trejo A; Blau HM. 2003. Stable reprogrammed heterokaryons form spontaneously in Purkinje neurons after bone marrow transplant. Nat Cell Biol 5(11):959-66. [PubMed: 14562057] [MGI Ref ID J:93485]
Zhang Y; Hayashi Y; Cheng X; Watanabe T; Wang X; Taniguchi N; Honke K. 2005. Testis-specific sulfoglycolipid, seminolipid, is essential for germ cell function in spermatogenesis. Glycobiology 15(6):649-54. [PubMed: 15659616] [MGI Ref ID J:133875]
Zhang Y; Jones M; McCabe A; Winslow GM; Avram D; Macnamara KC. 2013. MyD88 Signaling in CD4 T Cells Promotes IFN-gamma Production and Hematopoietic Progenitor Cell Expansion in Response to Intracellular Bacterial Infection. J Immunol 190(9):4725-35. [PubMed: 23526822] [MGI Ref ID J:195524]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice are bred as hemizygotes. The Donating Investigator maintained the line using a hemizygote x hemizygote breeding scheme and has not tried to make the strain homozygous. Mating System +/+ sibling x Hemizygote (Female x Male) 02-MAY-08 Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $261.00 Female or Male Hemizygous for Tg(CAG-EGFP)131Osb
Price per Pair (US dollars $) Pair Genotype $325.00 Hemizygous for Tg(CAG-EGFP)131Osb x Noncarrier $325.00 Noncarrier x Hemizygous for Tg(CAG-EGFP)131Osb Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $339.30 Female or Male Hemizygous for Tg(CAG-EGFP)131Osb
Price per Pair (US dollars $) Pair Genotype $422.50 Hemizygous for Tg(CAG-EGFP)131Osb x Noncarrier $422.50 Noncarrier x Hemizygous for Tg(CAG-EGFP)131Osb Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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|
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Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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