Strain Name: |
C57BL/6-Tg(CAG-EGFP)131Osb/LeySopJ |
|---|---|
Stock Number: |
006567 |
Availability: | Repository- Live |
General Terms and Conditions |
| Former Name |
C57BL/6-Tg(ACTB-EGFP)131Osb/LeySopJ (Changed: 08-MAY-08
) |
|
B6.Cg-Tg(ACTB-EGFP)1Osb/LeySopJ (Changed: 17-OCT-07
) | |
|
C57BL/6-Tg(ACTB-EGFP)1Osb/LeySopJ (Changed: 03-MAY-07
) | |
|
C57BL/6-Tg(CAGG-EGFP)1Ley/J (Changed: 03-MAY-07
) | |
| Genes & Alleles | ACTB; GFP; Tg(CAG-EGFP)131Osb; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Coisogenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Mating System +/+ sibling x Hemizygote (Female x Male) Species laboratory mouse Donating Investigator Brian Soper, The Jackson Laboratory Generation ?+F2 (20-DEC-07) Strain Description
This transgenic mouse line with an "enhanced" GFP (EGFP) cDNA under the control of a chicken beta-actin promoter and cytomegalovirus enhancer makes all of the tissues, with the exception of erythrocytes and hair, appear green under excitation light. The viability of homozygotes is unknown.Strain Development
The EcoRI sites included in the PCR primers were used to introduce the amplified EGFP cDNA into a pCAGGS expression vector containing the chicken beta-actin promoter and cytomegalovirus enhancer, beta-actin intron and bovine globin poly-adenylation signal. The entire insert with the promoter and coding sequence was excised with Bam-HI and SalI and gel-purified. The transgenic construct was was introduced into C57BL/6 donor eggs. Founder mice were bred to C57BL/6 mice, and backcrossed to the same for 11 generations.
Gene & Allele Details
| Allele Symbol | Tg(CAG-EGFP)131Osb | ||
|---|---|---|---|
| Allele Name | transgene insertion 131, Research Institute for Microbial Diseases, Osaka University | ||
| Common Name(s) | Tg(ACTB-EGFP)131Osb; Tg(ACTB-EGFP)Y01Osb; Tg(Act-EGFP)C14-Y01-FM131Osb; Tg(CAG-EGFP)C14-Y01-FM131Osb; Tg(act-EGFP)Y01Osb; Tg14(act-EGFP)ObsY01; TgN(act-EGFP)ObsC14-Yo1-FM1310; Y01; | ||
| Strain of Origin | C57BL/6 | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. The original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Promoter | ACTB, actin, beta, human | ||
| General Note | This transgene was made using the same construct as Tg(ACTB-EGFP)1Osb, but in this case it was injected into C57BL/6 fertilized oocytes. According to the Center for Animal Resources and Development Database (CARD R-Base), which distributes it, the transgene integrated on Chr 14D1. | ||
| Molecular Note | The transgene comprises the cDNA encoding enhanced green fluorescent protein (EGFP), from the jellyfish Aequoria victoria, downstream of the "CAG promoter" -- which consists of the cytomegalovirus immediate early (CMV-EI) enhancer followed by a 1.3-kb DNA segment including the promoter, first exon and first intron of the chicken beta-actin gene, with the 3' splice junction sequence replaced by that of the rabbit hemoglobin beta gene -- and followed by the rabbit beta-hemoglobin polyadenylation signal and 3' flanking sequence. [J:87415] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, these mice are bred as hemizygotes. The Donating Investigator maintained the line using a hemizygote x hemizygote breeding scheme and has not tried to make the strain homozygous. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (135 strains)
005703 B6.Cg-Tg(ACTFLPe)9205Dym/J 006055 B6.Cg-Tg(CAG-Bgeo,-DsRed*MST)1Nagy/J 003800 B6;SJL-Tg(ACTFLPe)9205Dym/J 005145 C57BL/6-Tg(CAG-OVA)916Jen/J 005863 C57BL/6J-Tg(ACTB-DDAH1)1Jpck/J 002981 DBA/2-Tg(xstpx-lacZ)36And/J 003376 FVB/N-Tg(ACTB-cre)2Mrt/J 005438 STOCK Tg(CAG-Bgeo,-DsRed*MST)1Nagy/J
Additional Web Information
Congenic Nomenclature
Fluorescent Proteins/lacZ Systems
Animal Health Reports
Room Number AX11
Research Applications
This mouse can be used to support research in many areas including:
GFP relatedResearch Tools
Fluorescent Proteins
Research Tools
Fluorescent Proteins
References
Selected Reference(s)
Additional ReferencesOkabe M; Ikawa M; Kominami K; Nakanishi T; Nishimune Y. 1997. 'Green mice' as a source of ubiquitous green cells. FEBS Lett 407(3):313-9. [PubMed: 9175875] [J:87415]
Price and Supply Information
| Strain Name: | C57BL/6-Tg(CAG-EGFP)131Osb/LeySopJ |
| Stock Number: | 006567 |
Price Details
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Supply Details
| Standard Supply | Repository-Live. No age specifications accepted. Colony sized to produce minimal quantities (typically up to 6 mice) upon order receipt; one order per strain. Expected delivery: 1-3 months. Larger quantities or custom orders arranged upon request. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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