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Type Coisogenic; Mutant Strain; Targeted Mutation; Mating System Heterozygote x Heterozygote (Female x Male) Species laboratory mouse Generation N9+N1F3 (20-MAY-08) Donating Investigator Susumu Tonegawa, Massachusetts Institute of Technology Description
Mice homozygous for this "fCNB1" mutant allele are viable and fertile with no reported neurological abnormalities. These mutant mice have loxP sites flanking exons 2-4 of the endogenous gene. When bred to transgenic mice expressing Cre recombinase, exons 2-4 are deleted in the resulting offspring in only those tissues expressing cre. For example, when crossed with transgenic mice expressing Cre recombinase specifically in the forebrain (similar to Stock No. 005359), the resulting offspring show abnormalities that are strikingly similar to those described for schizophrenia. These "floxed" mice may be useful in studies of calcineurin function in T cells (via NFAT transcription of cytokine genes) and in the central nervous system in, for example, neurite extension, synaptic plasticity, learning and memory, and schizophrenia pathogenesis.Development
A targeting vector was designed to insert a loxP site into intron 1 and a loxP- and FRT-flanked LFNT (pgk-neo and hsv-tk) cassette into intron 4 of the endogenous gene. The construct was electroporated into the C57BL/6-derived Bruce 4 embryonic stem (ES) cells, which were transiently transfected to remove the LFNT selection cassette. Correctly targeted ES cells (with loxP sites flanking exons 2-4 and a single remaining FRT site just upstream of the 3' loxP site) were injected into BALB/c blastocysts. The resulting chimeric mice were bred to C57BL/6 to establish the colony, and the strainwas backcrossed to C57BL/6 mice for at least 9 generations prior to arrival at The Jackson Laboratory.
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Cre-lox Systems
Research Applications
This mouse can be used to support research in many areas including:
Immunology and Inflammation Research
Growth Factors/Receptors/Cytokines
Neurobiology Research
Behavioral and Learning Defects
Cre-lox System (loxP-flanked Sequences)
Neurotransmitter Receptor and Synaptic Vesicle Defects
Research Tools
Cre-lox System (loxP-flanked Sequences)
Immunology and Inflammation Research
| Allele Symbol | Ppp3r1tm1Stl | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Susumu Tonegawa | ||
| Common Name(s) | fCNB1; | ||
| Mutation Made By | Susumu Tonegawa, Massachusetts Institute of Technology | ||
| Strain of Origin | C57BL/6 | ||
| ES Cell Line Name | Bruce 4 | ||
| ES Cell Line Strain | C57BL/6 | ||
| Gene Symbol and Name | Ppp3r1, protein phosphatase 3, regulatory subunit B, alpha isoform (calcineurin B, type I) | ||
| Chromosome | 11 | ||
| Gene Common Name(s) | CALNB1; CNB; CNB1; PP2B beta 1; | ||
| Molecular Note | Homologous recombination flanked the three major coding exons, exons 2-4, with a loxP site and a LFNT cassette. The LFNT cassette consisted of PGK-neomycin and HSV-thymidine kinase selection sequences flanked by a pair of loxP sites and a pair of frt sites. Cre- or Flp-mediated recombination in ES cells removed the PGK-neomycin and HSV-thymidine kinase sequences prior to production of chimeras. [MGI Ref ID J:73203] | ||
Genotyping Protocols
Ppp3r1tm1Stl, , vers. 1
Helpful Links
Optimizing PCR Protocols
Zeng H; Chattarji S; Barbarosie M; Rondi-Reig L; Philpot BD; Miyakawa T; Bear MF; Tonegawa S. 2001. Forebrain-Specific Calcineurin Knockout Selectively Impairs Bidirectional Synaptic Plasticity and Working/Episodic-like Memory. Cell 107(5):617-29. [PubMed: 11733061] [MGI Ref ID J:73203]
Ppp3r1tm1Stl related
Chan GC; Tonegawa S; Storm DR. 2005. Hippocampal neurons express a calcineurin-activated adenylyl cyclase. J Neurosci 25(43):9913-8. [PubMed: 16251439] [MGI Ref ID J:123451]Miyakawa T; Leiter LM; Gerber DJ; Gainetdinov RR; Sotnikova TD; Zeng H; Caron MG; Tonegawa S. 2003. Conditional calcineurin knockout mice exhibit multiple abnormal behaviors related to schizophrenia. Proc Natl Acad Sci U S A 100(15):8987-92. [PubMed: 12851457] [MGI Ref ID J:84640]
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, homozygous mice are bred. Diet Information LabDiet® 5K52/5K67
Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $155.70 Female or Male Homozygous for Ppp3r1tm1Stl *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $311.40 Homozygous for Ppp3r1tm1Stl x Homozygous for Ppp3r1tm1Stl
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $202.50 Female or Male Homozygous for Ppp3r1tm1Stl *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $404.90 Homozygous for Ppp3r1tm1Stl x Homozygous for Ppp3r1tm1Stl
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
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| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
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