Strain Name: |
B6;129S2-Omptm1Mom/MomJ |
|---|---|
Stock Number: |
006594 |
Availability: | Repository- Live |
General Terms and Conditions |
| Strain Common Names | OMP-tau-lacZ; |
| Genes & Alleles | Omp; Omptm1Mom; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Targeted Mutation Mating System +/+ sibling x Heterozygote (Female x Male) Species laboratory mouse Donating Investigator Peter Mombaerts, Max Planck Institute of Biophysics Strain Description
The coding region and part of the 3' non-translated region of the Omp gene was replaced by taulacZ, a fusion protein of the microtubule-binding protein tau (from bovine origin) and beta-galactosidase. The targeted mutation results in a knockout. Mature olfactory sensory neurons express beta-galactosidase at high levels. These neurons can be revealed by histochemistry of beta-galactosidase enzymatic activity, or by immunofluorescence with anti-beta-galactosidase antibodies. Homozygous mice are subfertile.Strain Development
The Omptm1Mom targeted mutation was generated in 129S2/SvPas-derived D3 ES cells by replacing the single Omp coding exon with tau-lacZ followed by an LTNL cassette. After expanding targeted clone# B38 the LTNL was excised by transiently transfecting with a plasmid encoding CMV-cre recombinase and these cells were negatively selected for recombination using ganciclovir. Clone B38/CreA5, which had the LTNL excised, was injected into C57BL/6 blastocysts and the resulting strain was interbred to homozygosity on this mixed B6;129S2 background.
Gene & Allele Details
| Allele Symbol | Omptm1Mom | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Peter Mombaerts | ||
| Common Name(s) | (OMP)-TLZ-; OMP-taulacZ; Omp-lacZ; | ||
| Mutation Made By | Peter Mombaerts, Max Planck Institute of Biophysics | ||
| Strain of Origin | 129S2/SvPas | ||
| ES Cell Line Name | D3 | ||
| ES Cell Line Strain | 129S2/SvPas | ||
| Gene Symbol and Name | Omp, olfactory marker protein | ||
| Chromosome | 7 | ||
| Molecular Note | Insertion of a tau-lacZ-loxP-tk-neo-loxP expression cassette deleted the coding exon and placed the tau-lacZ sequences under control of the endogenous regulatory sequences. Transient expression of Cre recombinase in C57BL/6 blastocysts excised the thymidine kinase and neomycin sequences. Expression of the tau-lacZ fusion protein in heterozygous mutant mice was detected in olfactory axon termini in individual glomeruli. [MGI Ref ID J:36595] | ||
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Omptm1Mom
Colony Maintenance
| Diet Information | LabDiet® 5K52/5K67 |
|---|
Related Strains
Strains carrying Omptm1Mom allele
007768 B6.129S2-Omptm1Mom/MomJ View Strains carrying Omptm1Mom (1 strain)
004946 B6;129P2-Omptm2(spH)Mom/J 006667 B6;129P2-Omptm3Mom/MomJ 006668 B6;129P2-Omptm4(cre)Mom/MomJ
Animal Health Reports
Room Number AX12
Research Applications
This mouse can be used to support research in many areas including:Omptm1Mom related
Research Tools
Sensorineural Research
Sensorineural Research
References
Selected Reference(s)
Additional ReferencesMombaerts P; Wang F; Dulac C; Chao SK; Nemes A; Mendelsohn M; Edmondson J; Axel R. 1996. Visualizing an olfactory sensory map. Cell 87(4):675-86. [PubMed: 8929536] [MGI Ref ID J:36595]
Price and Supply Information
| Strain Name: | B6;129S2-Omptm1Mom/MomJ |
| Stock Number: | 006594 |
Price Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
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- Use of MICE by companies or for-profit entities requires a license prior to shipping.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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