Strain Name: |
B6;129P2-Olfr17tm1Mom/MomJ |
|---|---|
Stock Number: |
006595 |
Availability: | Repository- Live |
General Terms and Conditions |
| Strain Common Names | P2-IRES-taulacZ; |
| Genes & Alleles | Olfr17; Olfr17tm1Mom; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Targeted Mutation Mating System Heterozygote x Heterozygote (Female x Male) Species laboratory mouse Donating Investigator Peter Mombaerts, The Rockefeller University Generation ?+N1 (11-JUL-07) Strain Description
Olfactory sensory neurons that express the olfactory receptor Olfr17 also co-express the taulacZ fusion protein by virtue of IRES-mediated co-translation. These neurons can be revealed by histochemistry of beta-galactosidase enzymatic activity, or by immunofluorescence with anti-beta-galactosidase antibodies.Strain Development
The homologous recombination that inserted the IRES-taulacZ-LTNL cassette downstream of the endogenous coding sequence was done in D3 ES cells, which were derived from 129S2/SvPas. The loxP-flanked neo cassette was then removed by transient transfection of a plasmid expressing cre recombinase, prior to injection of these ES cells into blastocysts. Resulting mice were bred to C57BL/6 and intercrossed.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Olfr17tm1Mom | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Peter Mombaerts | ||
| Common Name(s) | OR-lacZ; P2*-LacZ; P2-IRES-taulacZ; | ||
| Mutation Made By | Peter Mombaerts, Max Planck Institute of Biophysics | ||
| Strain of Origin | 129P2/OlaHsd | ||
| ES Cell Line Name | E14 | ||
| ES Cell Line Strain | 129P2/OlaHsd | ||
| Gene Symbol and Name | Olfr17, olfactory receptor 17 | ||
| Chromosome | 7 | ||
| Gene Common Name(s) | GA_x6K02T2PBJ9-9470146-9471093; JCG5; MOR263-5; OR10A4P; P2; ratchr1-164484422-164485369_ORF; | ||
| Molecular Note | Insertion of an IRES-tau-lacZ-loxP-tk-neo-loxP expression cassette placed the inserted sequences under control of the endogenous regulatory sequences. Transient expression of Cre recombinase in C57BL/6 blastocysts excised the thymidine kinase and neomycin sequences. This allele expresses a bicistronic mRNA encoding the receptor and a bovine tau-beta-galactosidase fusion protein. [MGI Ref ID J:36595] [MGI Ref ID J:99181] | ||
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Olfr17tm1Mom
Colony Maintenance
| Diet Information | LabDiet® 5K52/5K67 |
|---|
Related Strains
Strains carrying Olfr17tm1Mom allele
007767 B6.129P2-Olfr17tm1Mom/MomJ View Strains carrying Olfr17tm1Mom (1 strain)
006649 B6;129P2-Olfr17tm5Mom/MomJ 006650 STOCK Olfr17tm6Mom/MomJ 006669 STOCK Olfr17tm7Mom/MomJ 006651 STOCK-Olfr17tm4Mom/MomJ
Animal Health Reports
Room Number AX12
Research Applications
This mouse can be used to support research in many areas including:Olfr17tm1Mom related
Research Tools
Sensorineural Research
Sensorineural Research
References
Selected Reference(s)
Additional ReferencesMombaerts P; Wang F; Dulac C; Chao SK; Nemes A; Mendelsohn M; Edmondson J; Axel R. 1996. Visualizing an olfactory sensory map. Cell 87(4):675-86. [PubMed: 8929536] [MGI Ref ID J:36595]
Price and Supply Information
| Strain Name: | B6;129P2-Olfr17tm1Mom/MomJ |
| Stock Number: | 006595 |
Price Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
OncoMouse® requires a license from DuPont, see Licenses for Strains with OncoMouse® Technology.
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- Use of MICE by companies or for-profit entities requires a license prior to shipping.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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