Strain Name: |
B6.129S1-Mnx1tm4(cre)Tmj/J |
|---|---|
Stock Number: |
006600 |
Availability: | Repository- Live |
General Terms and Conditions |
| Former Name |
B6;129S-Mnx1tm4(cre)Tmj/J (Changed: 07-SEP-07
) |
|
B6;129S-Hlxb9tm4(cre)Tmj/J (Changed: 17-AUG-07
) | |
| Genes & Alleles | Mnx1; Mnx1tm4(cre)Tmj; cre; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Stock Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Mating System +/+ sibling x Heterozygote (Female x Male) Species laboratory mouse Donating Investigator Thomas Jessell, Columbia University/HHMI Generation N5+N3 (07-MAR-08) Strain Description
Mice heterozygous for this HB9cre targeted mutation are viable and fertile, with cre expression replacing HB9 (Hlxb9 or Mnx1) expression. Under control of the endogenous upstream elements, cre expression is directed to motor neurons. In heterozygotes, cre expression coincides with HB9 expression. Homozygous HB9cre mice die at or soon after birth, with expression of Cre recombinase likewise directed to motor neurons but no expression of endogenous HB9. When these HB9cre mice are bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination in the resulting offspring leads to deletion of the flanked sequences in Mnx1/HB9 expressing cells; making them useful in neurodevelopmental studies of homeobox genes, motor neuron function and differentiation, and the central nervous system.Strain Development
A targeting vector containing an internal ribosome entry site (IRES)-Cre cassette, SV40 polyA sequence, and loxP-flanked pgk-neo cassette was designed to replace a portion of the first exon of the targeted gene. The construct was electroporated into 129S1/Sv-derived W9.5 embryonic stem (ES) cells. Recombinant clones were injected into C57BL/6J blastocysts to generate chimeric founders that transmitted the mutant allele. These HB9cre mice were then backcrossed for at least 5 generations prior to arrival at The Jackson Laboratory.
Gene & Allele Details
| Allele Symbol | Mnx1tm4(cre)Tmj | ||
|---|---|---|---|
| Allele Name | targeted mutation 4, Thomas M Jessell | ||
| Common Name(s) | HB9cre; Hb9-CRE; | ||
| Mutation Made By | Thomas Jessell, Columbia University/HHMI | ||
| Strain of Origin | 129S1/Sv-Oca2<+> Tyr<+> Kitl<+> | ||
| ES Cell Line Name | W9.5/W95 | ||
| ES Cell Line Strain | 129S1/Sv-Oca2<+> Tyr<+> Kitl<+> | ||
| Site of Expression | motor neurons | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Gene Symbol and Name | Mnx1, motor neuron and pancreas homeobox 1 | ||
| Chromosome | 5 | ||
| Gene Common Name(s) | HB9; HLXB9; HOXHB9; Hlxb9; MNR2; SCRA1; homeobox gene HB9; | ||
| Molecular Note | Exon 1 was disrupted by the insertion of a cassette containing an IRES-tau-cre gene, a floxed neomycin gene, and a SV40 polyadenylation signal. [MGI Ref ID J:69623] | ||
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Generic Cre
Mnx1tm4(cre)Tmj
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, heterozygous mice may be bred to wildtype siblings or to C57BL/6J. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying other alleles of Mnx1
005029 B6.Cg-Tg(Hlxb9-GFP)1Tmj/J 006570 STOCK Smn1tm1Msd Tg(Hlxb9-GFP)1Tmj Tg(SMN2)89Ahmb/J View Strains carrying other alleles of Mnx1 (2 strains)
Additional Web Information
Congenic Nomenclature
Cre-lox or FLP-FRT Systems
Animal Health Reports
Room Number AX11
Research Applications
This mouse can be used to support research in many areas including:
cre relatedDevelopmental Biology Research
Neurodevelopmental Defects
Perinatal Lethality (Homozygous)
Neurobiology Research
Cre-lox System (Cre Recombinase expression in neural tissue)
Neurodevelopmental Defects
Research Tools
Cre-lox System (Cre Recombinase Expression)
Developmental Biology Research (Cre-lox System)
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
Genetics Research (Tissue/Cell Markers: Cre-lox System)
Neurobiology Research (cell marker)
Research Tools
Cre-lox System
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
References
Selected Reference(s)
Additional ReferencesArber S; Han B; Mendelsohn M; Smith M; Jessell TM; Sockanathan S. 1999. Requirement for the homeobox gene Hb9 in the consolidation of motor neuron identity [see comments] Neuron 23(4):659-74. [PubMed: 10482234] [MGI Ref ID J:57340]
Yang X; Arber S; William C; Li L; Tanabe Y; Jessell TM; Birchmeier C; Burden SJ. 2001. Patterning of muscle acetylcholine receptor gene expression in the absence of motor innervation. Neuron 30(2):399-410. [PubMed: 11395002] [MGI Ref ID J:69623]
Price and Supply Information
| Strain Name: | B6.129S1-Mnx1tm4(cre)Tmj/J |
| Stock Number: | 006600 |
Price Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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