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| When these HB9cre mice are bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination in the resulting offspring leads to deletion of the flanked sequences in Mnx1/HB9 expressing cells; making them useful in neurodevelopmental studies of homeobox genes, motor neuron function and differentiation, and the central nervous system. | |||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Former Names B6;129S-Mnx1tm4(cre)Tmj/J (Changed: 07-SEP-07 ) B6;129S-Hlxb9tm4(cre)Tmj/J (Changed: 17-AUG-07 ) Type Congenic; Mutant Stock; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System +/+ sibling x Heterozygote (Female x Male) 19-OCT-07 Species laboratory mouse Generation N5+N5F0 (19-MAY-09) Donating Investigator Thomas Jessell, Columbia University/HHMI Description
Mice heterozygous for this HB9cre targeted mutation are viable and fertile, with cre expression replacing HB9 (Hlxb9 or Mnx1) expression. Under control of the endogenous upstream elements, cre expression is directed to motor neurons. In heterozygotes, cre expression coincides with HB9 expression. Homozygous HB9cre mice die at or soon after birth, with expression of Cre recombinase likewise directed to motor neurons but no expression of endogenous HB9. When these HB9cre mice are bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination in the resulting offspring leads to deletion of the flanked sequences in Mnx1/HB9 expressing cells; making them useful in neurodevelopmental studies of homeobox genes, motor neuron function and differentiation, and the central nervous system.Development
A targeting vector containing an internal ribosome entry site (IRES)-Cre cassette, SV40 polyA sequence, and loxP-flanked pgk-neo cassette was designed to replace a portion of the first exon of the targeted gene. The construct was electroporated into 129S1/Sv-derived W9.5 embryonic stem (ES) cells. Recombinant clones were injected into C57BL/6J blastocysts to generate chimeric founders that transmitted the mutant allele. These HB9cre mice were then backcrossed for at least 5 generations prior to arrival at The Jackson Laboratory.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Mnx1
005029 B6.Cg-Tg(Hlxb9-GFP)1Tmj/J 006570 STOCK Smn1tm1Msd Tg(Hlxb9-GFP)1Tmj Tg(SMN2)89Ahmb/J View Strains carrying other alleles of Mnx1 (2 strains)
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
cre relatedDevelopmental Biology Research
Neurodevelopmental Defects
Perinatal Lethality
Homozygous
Neurobiology Research
Cre-lox System
Cre Recombinase expression in neural tissue
Neurodevelopmental Defects
Research Tools
Cre-lox System
Cre Recombinase Expression
Developmental Biology Research
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Tissue/Cell Markers: Cre-lox System
Neurobiology Research
cell marker
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Mnx1tm4(cre)Tmj | ||
|---|---|---|---|
| Allele Name | targeted mutation 4, Thomas M Jessell | ||
| Allele Type | Targeted (knock-in) | ||
| Common Name(s) | HB9cre; Hb9-CRE; | ||
| Mutation Made By | Thomas Jessell, Columbia University/HHMI | ||
| Strain of Origin | 129S1/Sv-Oca2<+> Tyr<+> Kitl<+> | ||
| ES Cell Line Name | W9.5/W95 | ||
| ES Cell Line Strain | 129S1/Sv-Oca2<+> Tyr<+> Kitl<+> | ||
| Site of Expression | motor neurons | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Gene Symbol and Name | Mnx1, motor neuron and pancreas homeobox 1 | ||
| Chromosome | 5 | ||
| Gene Common Name(s) | HB9; HLXB9; HOXHB9; Hlxb9; MNR2; SCRA1; homeobox gene HB9; | ||
| Driver Note | Mnx1 | ||
| Molecular Note | Exon 1 was disrupted by the insertion of a cassette containing an IRES-tau-cre gene, a floxed neomycin gene, and a SV40 polyadenylation signal. [MGI Ref ID J:69623] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic Cre Melt Curve Analysis, Melt Curve Analysis
Generic Cre, Standard PCR
Mnx1tm4(cre)Tmj, Separated PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Arber S; Han B; Mendelsohn M; Smith M; Jessell TM; Sockanathan S. 1999. Requirement for the homeobox gene Hb9 in the consolidation of motor neuron identity [see comments] Neuron 23(4):659-74. [PubMed: 10482234] [MGI Ref ID J:57340]
Yang X; Arber S; William C; Li L; Tanabe Y; Jessell TM; Birchmeier C; Burden SJ. 2001. Patterning of muscle acetylcholine receptor gene expression in the absence of motor innervation. Neuron 30(2):399-410. [PubMed: 11395002] [MGI Ref ID J:69623]
Additional References
Mnx1tm4(cre)Tmj relatedBloom AJ; Miller BR; Sanes JR; Diantonio A. 2007. The requirement for Phr1 in CNS axon tract formation reveals the corticostriatal boundary as a choice point for cortical axons. Genes Dev 21(20):2593-606. [PubMed: 17901218] [MGI Ref ID J:125702]
Bolis A; Coviello S; Bussini S; Dina G; Pardini C; Previtali SC; Malaguti M; Morana P; Del Carro U; Feltri ML; Quattrini A; Wrabetz L; Bolino A. 2005. Loss of Mtmr2 phosphatase in Schwann cells but not in motor neurons causes Charcot-Marie-Tooth type 4B1 neuropathy with myelin outfoldings. J Neurosci 25(37):8567-77. [PubMed: 16162938] [MGI Ref ID J:101056]
Gould TW; Yonemura S; Oppenheim RW; Ohmori S; Enomoto H. 2008. The neurotrophic effects of glial cell line-derived neurotrophic factor on spinal motoneurons are restricted to fusimotor subtypes. J Neurosci 28(9):2131-46. [PubMed: 18305247] [MGI Ref ID J:132854]
Hippenmeyer S; Huber RM; Ladle DR; Murphy K; Arber S. 2007. ETS transcription factor Erm controls subsynaptic gene expression in skeletal muscles. Neuron 55(5):726-40. [PubMed: 17785180] [MGI Ref ID J:126807]
Hippenmeyer S; Vrieseling E; Sigrist M; Portmann T; Laengle C; Ladle DR; Arber S. 2005. A developmental switch in the response of DRG neurons to ETS transcription factor signaling. PLoS Biol 3(5):e159. [PubMed: 15836427] [MGI Ref ID J:100886]
Jevsek M; Jaworski A; Polo-Parada L; Kim N; Fan J; Landmesser LT; Burden SJ. 2006. CD24 is expressed by myofiber synaptic nuclei and regulates synaptic transmission. Proc Natl Acad Sci U S A 103(16):6374-9. [PubMed: 16606832] [MGI Ref ID J:109028]
Kim N; Burden SJ. 2008. MuSK controls where motor axons grow and form synapses. Nat Neurosci 11(1):19-27. [PubMed: 18084289] [MGI Ref ID J:129239]
Kramer ER; Knott L; Su F; Dessaud E; Krull CE; Helmbacher F; Klein R. 2006. Cooperation between GDNF/Ret and ephrinA/EphA4 signals for motor-axon pathway selection in the limb. Neuron 50(1):35-47. [PubMed: 16600854] [MGI Ref ID J:110955]
Li XM; Dong XP; Luo SW; Zhang B; Lee DH; Ting AK; Neiswender H; Kim CH; Carpenter-Hyland E; Gao TM; Xiong WC; Mei L. 2008. Retrograde regulation of motoneuron differentiation by muscle beta-catenin. Nat Neurosci 11(3):262-8. [PubMed: 18278041] [MGI Ref ID J:135587]
Patel TD; Kramer I; Kucera J; Niederkofler V; Jessell TM; Arber S; Snider WD. 2003. Peripheral NT3 signaling is required for ETS protein expression and central patterning of proprioceptive sensory afferents. Neuron 38(3):403-16. [PubMed: 12741988] [MGI Ref ID J:83461]
Pun S; Sigrist M; Santos AF; Ruegg MA; Sanes JR; Jessell TM; Arber S; Caroni P. 2002. An intrinsic distinction in neuromuscular junction assembly and maintenance in different skeletal muscles. Neuron 34(3):357-70. [PubMed: 11988168] [MGI Ref ID J:76364]
Zhang L; Schessl J; Werner M; Bonnemann C; Xiong G; Mojsilovic-Petrovic J; Zhou W; Cohen A; Seeburg P; Misawa H; Jayaram A; Personius K; Hollmann M; Sprengel R; Kalb R. 2008. Role of GluR1 in activity-dependent motor system development. J Neurosci 28(40):9953-68. [PubMed: 18829953] [MGI Ref ID J:141815]
Zhou W; Zhang L; Guoxiang X; Mojsilovic-Petrovic J; Takamaya K; Sattler R; Huganir R; Kalb R. 2008. GluR1 controls dendrite growth through its binding partner, SAP97. J Neurosci 28(41):10220-33. [PubMed: 18842882] [MGI Ref ID J:141127]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, heterozygous mice may be bred to wildtype siblings or to C57BL/6J. Mating System +/+ sibling x Heterozygote (Female x Male) 19-OCT-07 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $243.50 Female or Male Heterozygous for Mnx1tm4(cre)Tmj
Pairs /Price (US dollars $) Pair Genotype $297.85 Heterozygous for Mnx1tm4(cre)Tmj x Wild-type for Mnx1tm4(cre)Tmj $297.85 Wild-type for Mnx1tm4(cre)Tmj x Heterozygous for Mnx1tm4(cre)Tmj
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $316.60 Female or Male Heterozygous for Mnx1tm4(cre)Tmj
Pairs /Price (US dollars $) Pair Genotype $387.30 Heterozygous for Mnx1tm4(cre)Tmj x Wild-type for Mnx1tm4(cre)Tmj $387.30 Wild-type for Mnx1tm4(cre)Tmj x Heterozygous for Mnx1tm4(cre)Tmj
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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