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Strain Name:

B6.129-Pctptm1Bor/J

Stock Number:

006607

Availability:

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Genes & Alleles   Pctp;   Pctptm1Bor;

Product Information

Strain Details

Type JAX® GEMM® Strain - Congenic
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Mutant Strain
Type JAX® GEMM® Strain - Targeted Mutation
Mating SystemHomozygote x Homozygote         (Female x Male)
Specieslaboratory mouse
Donating Investigator David Cohen,   Brigham and Women's Hospital
GenerationN21+F3 (03-DEC-07)

Strain Description
Mice homozygous for this targeted mutation are viable and fertile and do not display any gross physical or behavioral abnormalities. Protein product from the targeted gene is not detected in the liver cytosol of 8-day-old homozygous pups. The lipid content and composition of bile and lung surfactant secretions are normal in homozygous targeted mice. Plasma cholesterol and phospholipid levels are not affected in chow-fed homozygous targeted mutation mice, but there is an increase in the accumulation of small alpha-migrating high density lipoprotein (HDL) particles. Biliary concentrations of phospholipids, cholesterol, and bile salts are reduced in homozygous mutants as compared to wildtype mice when fed a high fat, high cholesterol, cholate-containing lithogenic diet. There is a greater hepatic accumulation of phospholipid and cholesterol in the targeted mutant animals. On the high fat diet, HDL particles are of normal size, but plasma cholesterol and phospholipid concentrations are increased compared to wildtype mice. This strain represents a model that may be useful in studies of lipid metabolism.

Strain Development
A targeting vector containing a neomycin resistance gene was used to replace exons C and D by homologous recombination. The construct was electroporated into 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. The resultant strain has been backcrossed approximately twenty times to C57BL/6 by the donating laboratory.

Mammalian Phenotype Terms assigned by genotype

Pctptm1Bor/Pctptm1Bor

        B6.129P2-Pctptm1Bor
  • cellular phenotype
  • increased apoptosis (MGI Ref ID J:115284)
    • macrophages loaded with free (unesterified) cholesterol (FC) show significantly more apoptotic cell death and total cell death compared to FC-loaded wild type macrophages
  • immune system phenotype
  • abnormal macrophage physiology (MGI Ref ID J:115284)
    • macrophages loaded with cholesteryl esters show impaired apoAI-mediated efflux of phospholipids and cholesterol compared to wild type macrophages
    • macrophages loaded with free (unesterified) cholesterol (FC) show significantly more apoptotic cell death and total cell death compared to FC-loaded wild type macrophages

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Pctptm1Bor/Pctptm1Bor

        involves: 129P2/OlaHsd * FVB
  • normal phenotype
  • no abnormal phenotype detected (MGI Ref ID J:57946)
    • mice do not exhibit defects in lipid metabolism, liver function or lung surfactant production

Pctptm1Bor/Pctptm1Bor

        involves: 129P2/OlaHsd * C57BL/6J
  • liver/biliary system phenotype
  • abnormal bile duct morphology (MGI Ref ID J:115285)
    • in mutant livers, bile canaliculi show extensive branching at membrane surface
  • abnormal gall bladder physiology (MGI Ref ID J:115285)
    • occlusion of gall bladder with mucin occurs at 7 days on a lithogenic diet compared to 18 hours on the FVB/NJ background
    • abnormal bile composition (MGI Ref ID J:115285)
      • phospholipids in bile of mice on a chow diet are 1.3 fold higher than littermates
      • on a lithogenic diet, after 7 days, mice have 1.3 fold lower phospholipid levels in bile
      • on a lithogenic diet, after 7 days, mice have 1.3 fold lower biliary cholesterol levels in bile compared to wild type littermates
    • gallstones (MGI Ref ID J:115285)
      • on a lithogenic diet, abundant liquid and solid cholesterol crystals that precede formation of macroscopic cholesterol gallstones are observed after 9 days
  • homeostasis/metabolism phenotype
  • abnormal bile salt level (MGI Ref ID J:115285)
    • on a lithogenic diet, TUDC concentration increases in mutants and controls, but remain higher in mutants; TC remains unchanged in mutants but increases in wild type
    • relative concentration of tauromuricholate (TMC) is reduced and tauroursdeoxycholate (TUDC) and taurocholate (TC) concentrations are increased compared to control
    • bile salt concentrations are 1.3 fold lower than controls on lithogenic diets compared to mutants on the FVB/NJ background
  • abnormal phospholipid level (MGI Ref ID J:115285)
    • phospholipids in bile of mice on a chow diet are 1.3 fold higher than littermates
    • on a lithogenic diet, after 7 days, mice have 1.3 fold lower phospholipid levels in bile

Pctptm1Bor/Pctptm1Bor

        involves: 129P2/OlaHsd * FVB/NJ
  • liver/biliary system phenotype
  • abnormal bile duct morphology (MGI Ref ID J:115285)
    • in mutant livers, bile canaliculi show extensive branching at membrane surface
    • bile canaliculi and junctional complexes appear more tortuous
  • abnormal gall bladder physiology (MGI Ref ID J:115285)
    • occlusion of gall bladder with mucin occurs at 18 hours on a lithogenic diet
    • occlusion occurs much more quickly than on the C57BL/6J background
    • abnormal bile secretion (MGI Ref ID J:115285)
      • bile flow rate is reduced by ~35% after 30 minutes in mutants on a regular chow diet
  • homeostasis/metabolism phenotype
  • abnormal bile salt level (MGI Ref ID J:115285)
    • bile salt concentrations are 4.3 fold higher than controls on regular chow diets
    • there is marked increase in bile salt secretion on chow diet
    • on lithogenic diet, concentration decreases; in wild type, concentrations increase such that both genotypes have similar bile salt concentrations
    • on a chow diet, TC concentration is higher than controls, while other bile salt concentrations are reduced; on a lithogenic diet, percentage of TUDC is higher in mutants, but other types are similar in both mutants and controls
  • decreased cholesterol level (MGI Ref ID J:115285)
    • on a lithogenic diet, biliary cholesterol levels decrease, but levels increase in wild type
  • increased cholesterol level (MGI Ref ID J:115285)
    • in chow-fed mice, biliary cholesterol levels are 5.9 fold higher than in controls

Gene & Allele Details

Allele Symbol Pctptm1Bor
Allele Name targeted mutation 1, Piet Borst
Common Name(s) Pc-tp -; Pctp-;
Mutation Made By Michele Wu,   Brigham and Women's Hospital
Strain of Origin129P2/OlaHsd
ES Cell Line NameE14
ES Cell Line Strain129P2/OlaHsd
Gene Symbol and Name Pctp, phosphatidylcholine transfer protein
Chromosome 11
Gene Common Name(s) PC-TP; STARD2;
Molecular Note A neomycin selection gene replaced exons C and D by homologous recombination. Western blot analysis demonstrated that no detectable protein product was made in the cytosol of cells derived from homozygous mice. [MGI Ref ID J:57946]

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Genotyping Protocols

Pctptm1Bor

Colony Maintenance

Diet Information LabDiet® 5K52/5K67

Related Strains

Strains carrying other alleles of Pctp
000684   NZB/BlNJ
002105   NZO/HlLtJ
001058   NZW/LacJ
View Strains carrying other alleles of Pctp     (3 strains)

Additional Web Information

Congenic Nomenclature

Animal Health Reports

Room Number           AX11

Research Applications

This mouse can be used to support research in many areas including:

Metabolism Research
Lipid Metabolism

References

Selected Reference(s)

van Helvoort A; de Brouwer A; Ottenhoff R; Brouwers JF; Wijnholds J; Beijnen JH; Rijneveld A; van der Poll T; van der Valk MA; Majoor D; Voorhout W; Wirtz KW; Elferink RP; Borst P. 1999. Mice without phosphatidylcholine transfer protein have no defects in the secretion of phosphatidylcholine into bile or into lung airspaces. Proc Natl Acad Sci U S A 96(20):11501-6. [PubMed: 10500206]  [MGI Ref ID J:57946]

Additional References

Price and Supply Information

Strain Name: B6.129-Pctptm1Bor/J
Stock Number: 006607

Price Details

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Supply Details

Standard SupplyRepository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes Usually shipped between four and eight weeks of age.
This strain is included in the Induced Mutant Resource Colony collection.
LicensingSee General Terms and Conditions below for Licensing and Use Restrictions  
Control InformationView Control Information in Strain Details.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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