Strain Name: |
STOCK Tg(CAG-Bgeo,-Tle1,-ALPP)1Lbe/J |
|---|---|
Stock Number: |
006613 |
Availability: | Repository- Live |
General Terms and Conditions |
| Former Name |
STOCK Tg(ACTB-Bgeo,-Tle1,-ALPP)1Lbe/J (Changed: 08-MAY-08
) |
| Genes & Alleles | ACTB; ALPP; Bgeo; Tle1; lacZ; Tg(CAG-Bgeo,-Tle1,-ALPP)1Lbe; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Transgenic Mating System +/+ sibling x Hemizygote (Female x Male) Species laboratory mouse Donating Investigator Corrinne Lobe, Sunnybrook & Women's College HSC Generation ?+F1 (10-JUL-07) Strain Description
Hemizygous transgenic mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express the mouse transducin-like enhancer of split 1, homolog of Drosophila E(spl) gene, Tle1 (also known as Groucho-related gene 1; Grg1) under the regulation of the chicken beta actin promoter (ACTB) and the cytomegalovirus (CMV) intermediate-early enhancer. An internal ribosome entry sequence (IRES) followed by the human alkaline phosphatase (ALPP) reporter gene inserted downstream of the Tle1 gene directs co-expression of ALPP and TLE1. TLE1 and ALPP co-expression is blocked, however, by the presence of a loxP-flanked Bgeo (beta-galactosidase/neomycin resistance fusion protein) cassette inserted between the ACTB/CMV promoter and the Tle1 coding sequence. In the absence of Cre recombinase, beta-galactosidase activity is detected in all tissues tested including pancreas, lung, kidney, cerebellum, heart, muscle and lens and retina. Diminished beta-galactosidase activity is observed in liver tissue. When transgenic mice are crossed with a Cre recombinase-expressing strain, lacZ expression in the resulting offspring is replaced by both ALPP and TLE1 protein in cre-expressing tissues. These mice may be useful in studying lung tumorigenesis.Strain Development
A transgenic construct containing the human alkaline phosphatase, placental (Regan isozyme), gene ALPP, the mouse transducin-like enhancer of split 1, homolog of Drosophila E(spl) gene Tle1, loxP site flanked Bgeo reporter (beta-galactosidase/neomycin resistance fusion gene)/SV40 polyadenylation signal segment and IRES (Internal Ribosomal Entry) sequence under the control of the a chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate early enhancer, was introduced into R1 embryonic stem (ES) cells. ES cell clones containing a single copy of the construct were used to generate aggregated chimeric mice. Resulting male chimeric animals were bred with CD1 female mice.
Gene & Allele Details
| Allele Symbol | Tg(CAG-Bgeo,-Tle1,-ALPP)1Lbe | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Corrinne Lobe | ||
| Common Name(s) | Grg1lacZ; Tg(ACTB-Bgeo,-Tle1,-ALPP)1Lbe; iZ/AP-Grg1; | ||
| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| ES Cell Line Name | R1 | ||
| ES Cell Line Strain | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| Expressed Gene | Tle1, transducin-like enhancer of split 1, homolog of Drosophila E(spl), mouse, laboratory | ||
| Expressed Gene | lacZ, beta-galactosidase, E. coli | ||
| Expressed Gene | ALPP, alkaline phosphatase, placental (Regan isozyme), human | ||
| Expressed Gene | Bgeo, fusion of beta-galactosidase and neomycin phosphotransferase genes , E. coli | ||
| Promoter | ACTB, actin, beta, chicken | ||
| Molecular Note | The transgene contains a strong cytomegalovirus (CMV) enhancer followed by the chicken beta-actin promoter; a loxP-flanked beta-galactosidase/neomycin resistance fusion (Bgeo) gene with three polyadenylation signals; the coding sequence of the mouse transducin-like enhancer of split 1, homolog of Drosophila E(spl) (Tle1) gene; and the human alkaline phosphatase, placental (Regan isozyme) (ALPP) gene preceded by an interal ribosome entry site (IRES). In mice doubly transgenic for this and a selected Cre recombinase (cre) transgene, excision of the floxed Bgeo results in whole-animal or tissue-specific co-expression of Tle1 and the ALPP reporter gene. [J:106684] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
Colony Maintenance
| Diet Information | LabDiet® 5K52/5K67 |
|---|
Related Strains
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View lacZ Expression Strains (174 strains)
007066 B6.SJL-Tg(Crx-GFP,-ALPP)1Clc/J 006882 STOCK Tg(CAG-Bgeo,-AML1/ETO,-ALPP)1Lbe/J 003919 STOCK Tg(CAG-Bgeo/ALPP)1Lbe/J 005105 STOCK Tg(Chx10-EGFP/cre-ALPP)2Clc/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Animal Health Reports
Room Number AX11
Research Applications
This mouse can be used to support research in many areas including:
Bgeo relatedCancer Research
Increased Tumor Incidence (Adenomas: lung, induced)
Cell Biology Research
Transcriptional Regulation
Developmental Biology Research
Research Tools
Developmental Biology Research
lacZ Expression
lacZ relatedResearch Tools
lacZ Expression
lacZ Expression
References
Selected Reference(s)
Allen T; van Tuyl M; Iyengar P; Jothy S; Post M; Tsao MS; Lobe CG. 2006. Grg1 acts as a lung-specific oncogene in a transgenic mouse model. Cancer Res 66(3):1294-301. [PubMed: 16452182] [J:106684]
Price and Supply Information
| Strain Name: | STOCK Tg(CAG-Bgeo,-Tle1,-ALPP)1Lbe/J |
| Stock Number: | 006613 |
Price Details
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Supply Details
| Standard Supply | Repository-Live. No age specifications accepted. Colony sized to produce minimal quantities (typically up to 6 mice) upon order receipt; one order per strain. Expected delivery: 1-3 months. Larger quantities or custom orders arranged upon request. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Mice are Only Available to Non Profit Research Institutions, Universities and Hospitals.
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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