Strain Name: |
STOCK Tg(CAG-Bgeo,-Tle1,-ALPP)1Lbe/J |
|---|---|
Stock Number: |
006613 |
Availability: | Repository- Live |
Price and Supply Information | |
General Terms and Conditions |
| Former Name |
STOCK Tg(ACTB-Bgeo,-Tle1,-ALPP)1Lbe/J (Changed: 08-MAY-08
) |
| Genes & Alleles | ACTB; ALPP; Bgeo; Tle1; lacZ; Tg(CAG-Bgeo,-Tle1,-ALPP)1Lbe; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Transgenic Mating System +/+ sibling x Hemizygote (Female x Male) Species laboratory mouse Donating Investigator Corrinne Lobe, Sunnybrook & Women's College HSC Generation ?+F1 (10-JUL-07) Strain Description
Hemizygous transgenic mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express the mouse transducin-like enhancer of split 1, homolog of Drosophila E(spl) gene, Tle1 (also known as Groucho-related gene 1; Grg1) under the regulation of the chicken beta actin promoter (ACTB) and the cytomegalovirus (CMV) intermediate-early enhancer. An internal ribosome entry sequence (IRES) followed by the human alkaline phosphatase (ALPP) reporter gene inserted downstream of the Tle1 gene directs co-expression of ALPP and TLE1. TLE1 and ALPP co-expression is blocked, however, by the presence of a loxP-flanked Bgeo (beta-galactosidase/neomycin resistance fusion protein) cassette inserted between the ACTB/CMV promoter and the Tle1 coding sequence. In the absence of Cre recombinase, beta-galactosidase activity is detected in all tissues tested including pancreas, lung, kidney, cerebellum, heart, muscle and lens and retina. Diminished beta-galactosidase activity is observed in liver tissue. When transgenic mice are crossed with a Cre recombinase-expressing strain, lacZ expression in the resulting offspring is replaced by both ALPP and TLE1 protein in cre-expressing tissues. These mice may be useful in studying lung tumorigenesis.Strain Development
A transgenic construct containing the human alkaline phosphatase, placental (Regan isozyme), gene ALPP, the mouse transducin-like enhancer of split 1, homolog of Drosophila E(spl) gene Tle1, loxP site flanked Bgeo reporter (beta-galactosidase/neomycin resistance fusion gene)/SV40 polyadenylation signal segment and IRES (Internal Ribosomal Entry) sequence under the control of the a chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate early enhancer, was introduced into R1 embryonic stem (ES) cells. ES cell clones containing a single copy of the construct were used to generate aggregated chimeric mice. Resulting male chimeric animals were bred with CD1 female mice.
Gene & Allele Details
| Allele Symbol | Tg(CAG-Bgeo,-Tle1,-ALPP)1Lbe | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Corrinne Lobe | ||
| Common Name(s) | Grg1lacZ; Tg(ACTB-Bgeo,-Tle1,-ALPP)1Lbe; iZ/AP-Grg1; | ||
| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| ES Cell Line Name | R1 | ||
| ES Cell Line Strain | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| Expressed Gene | Tle1, transducin-like enhancer of split 1, homolog of Drosophila E(spl), mouse, laboratory | ||
| Expressed Gene | lacZ, beta-galactosidase, E. coli | ||
| Expressed Gene | ALPP, alkaline phosphatase, placental (Regan isozyme), human | ||
| Expressed Gene | Bgeo, fusion of beta-galactosidase and neomycin phosphotransferase genes , E. coli | ||
| Promoter | ACTB, actin, beta, chicken | ||
| Molecular Note | The transgene contains a strong cytomegalovirus (CMV) enhancer followed by the chicken beta-actin promoter; a loxP-flanked beta-galactosidase/neomycin resistance fusion (Bgeo) gene with three polyadenylation signals; the coding sequence of the mouse transducin-like enhancer of split 1, homolog of Drosophila E(spl) (Tle1) gene; and the human alkaline phosphatase, placental (Regan isozyme) (ALPP) gene preceded by an interal ribosome entry site (IRES). In mice doubly transgenic for this and a selected Cre recombinase (cre) transgene, excision of the floxed Bgeo results in whole-animal or tissue-specific co-expression of Tle1 and the ALPP reporter gene. [MGI Ref ID J:106684] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for JAX® GEMM® Strains | ||
Colony Maintenance
| Diet Information | LabDiet® 5K52/5K67 |
|---|
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007066 B6.SJL-Tg(Crx-GFP,-ALPP)1Clc/J 006882 STOCK Tg(CAG-Bgeo,-AML1/ETO,-ALPP)1Lbe/J 003919 STOCK Tg(CAG-Bgeo/ALPP)1Lbe/J 005105 STOCK Tg(Chx10-EGFP/cre-ALPP)2Clc/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Animal Health Reports
Room Number AX11
Research Applications
This mouse can be used to support research in many areas including:
Bgeo relatedCancer Research
Increased Tumor Incidence (Adenomas: lung, induced)
Cell Biology Research
Transcriptional Regulation
Developmental Biology Research
Research Tools
lacZ Expression
Developmental Biology Research
lacZ relatedResearch Tools
lacZ Expression
lacZ Expression
References
Selected Reference(s)
Allen T; van Tuyl M; Iyengar P; Jothy S; Post M; Tsao MS; Lobe CG. 2006. Grg1 acts as a lung-specific oncogene in a transgenic mouse model. Cancer Res 66(3):1294-301. [PubMed: 16452182] [MGI Ref ID J:106684]
Price and Supply Information
| Strain Name: | STOCK Tg(CAG-Bgeo,-Tle1,-ALPP)1Lbe/J |
| Stock Number: | 006613 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. The shipping destinations are:
- USA, Canada and Mexico
- International
*Pricing for Shipping Destination selected:
International
| Price(s) in US dollars ($) | Genotype(s) Provided | ||||
|---|---|---|---|---|---|
| Individual Mouse Price | $307.40 | Hemizygous for Tg(CAG-Bgeo,-Tle1,-ALPP)1Lbe | |||
| Pair | $375.30 | Hemizygous for Tg(CAG-Bgeo,-Tle1,-ALPP)1Lbe x Noncarrier | |||
| Pair | $375.30 | Noncarrier x Hemizygous for Tg(CAG-Bgeo,-Tle1,-ALPP)1Lbe | |||
Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Histology and Tissue Collection Services are available for all JAX® Mice strains. For more information, please contact Customer Service at orderquest@jax.org or 1-207-288-5845. Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. View Control Pricing Information for JAX® Strains. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
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- Strain(s) not available to companies or for-profit entities.
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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