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Strain Name:

C57BL/6-Tg(tetO-COX8A/EYFP)1Ksn/J

Stock Number:

006618

Availability:

Repository- Live


General Terms and Conditions

Former Name      C57BL6-Tg(tetO-COX8A/EYFP)1Ksn/J    (Changed: 22-MAR-07 )
      C57BL6-Tg(TRE-COX8/EYFP)001Ksn/J    (Changed: 13-MAR-07 )
Genes & Alleles   COX8A;   YFP;   Tg(tetO-COX8A/EYFP)1Ksn;   tetO;


Product Information

Strain Details

Type JAX® GEMM® Strain - Coisogenic
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Mutant Strain
Type JAX® GEMM® Strain - Transgenic
Mating System+/+ sibling x Hemizygote         (Female x Male)
Specieslaboratory mouse
Donating Investigator Tibor Kristian,   University of Maryland
GenerationN7+ (09-JUL-07)

Strain Description
Hemizygotes are viable and fertile. These transgenic mice express a mitochondrial-specific EYFP fusion protein under the control of a tetracycline-responsive promoter element (TRE;tetO). When hemizygotes are bred with another transgenic mouse expressing either reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA) under the regulation of tissue-specific promoter, tissue-specific expression of EYFP in mitochondria of the bitransgenic offspring can be regulated with the tetracycline analog, doxycycline.

When bred to a strain expressing rtTA or tTA in forebrain neurons (see Stock No. 003010 for example), this mutant mouse strain may be useful in studies of neuronal mitochondrial dysfunction.

Strain Development
A transgenic fusion protein construct containing the mitochondrial targeting sequence from the human COX8 gene and Enhanced Yellow Fluorescent Protein (EYFP) was placed downstream of a tet-responsive promoter element (TRE) and upstream of a beta-globin poly A signal. The construct was injected into fertilized C57BL/6 eggs. Founder animals were bred to C57BL/6 for six generations by the donating investigator.

Mammalian Phenotype Terms assigned by genotype

The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.

Tg(Camk2a-tTA)1Mmay/0 Tg(tetO-COX8A/EYFP)1Ksn/0

        involves: C57BL/6
  • normal phenotype
  • no abnormal phenotype detected (J:118452)
    • mice appear normal and healthy, and expression of EYFP is detected in neuronal mitochondria under appropriate conditions

Gene & Allele Details

Allele Symbol Tg(tetO-COX8A/EYFP)1Ksn
Allele Name transgene insertion 1, Tibor Kristian
Common Name(s) TRE-mito/EYFP; pTRE2-mito/eYFP;
Mutation Made By Tibor Kristian,   University of Maryland
Strain of OriginC57BL/6
Site of Expressionmitochondrial-specific EYFP fusion protein under the control of a tetracycline-responsive promoter element (TRE or tetO)
Expressed Gene YFP, Yellow Fluorescent Protein, jellyfish
Yellow Fluorescent Protein (YFP) is a derivative of Green Fluorescent Protein (GFP), a versatile reporter molecule which has found use in many biological applications. The original molecule has been modified in order to enhance fluorescence intensity and shift the wavelength emitted when excited. When YFP is utilized in a transgenic construct, tissue expressing sufficient amounts of YFP will fluoresce yellowish-green when exposed to a 513 nm light source.
Expressed Gene COX8A, cytochrome c oxidase subunit 8A (ubiquitous), human
Promoter tetO, tet operator,
Molecular Note A construct encoding a fusion protein comprising the mitochondrial targeting sequence from human cytochrome c oxidase subunit 8A (ubiquitous) joined to enhanced yellow fluorescent protein (EYFP) was placed downstream of the tetracycline responsive element (tetO) and upsteam of a beta-globin polyadenylation signal. A mouse with this transgene can be bred with another transgenic mouse expressing reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA) under control of a selected promoter to produce bitransgenic mice in which mitochondrial EYFP expression can be regulated in a tissue-specific manner by administration/removal of the tetracycline analog, doxycycline. [J:118452]

Control Information

  Control
   Noncarrier
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Colony Maintenance

Diet Information LabDiet® 5K52/5K67

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View Fluorescent Protein Strains     (135 strains)

Strains carrying other alleles of COX8A
007940   B6.Cg-Tg(Thy1-CFP/COX8A)C1Lich/J
006614   B6;CB-Tg(Thy1-CFP/COX8A)C1Lich/J
006617   B6;CB-Tg(Thy1-CFP/COX8A)S2Lich/J
View Strains carrying other alleles of COX8A     (3 strains)

View Strains carrying other alleles of YFP     (18 strains)

View Strains carrying other alleles of tetO     (25 strains)

Additional Web Information

Fluorescent Proteins/lacZ Systems
Tet Expression Systems
Visit the Alzheimer's Disease Mouse Model Resource site for helpful information on Alzheimer's Disease and research resources.

Animal Health Reports

Room Number           AX11

Research Applications

This mouse can be used to support research in many areas including:

Neurobiology Research
Alzheimer's Disease
Fluorescent protein expression in neural tissue
Parkinson's Disease
Tet Expression System (tTA/rtTA Responsive Strains)

Research Tools
Fluorescent Proteins
Tet Expression Systems (tTA/rtTA Responsive Strains)

YFP related

Research Tools
Fluorescent Proteins

References

Selected Reference(s)

Chandrasekaran K; Hazelton JL; Wang Y; Fiskum G; Kristian T. 2006. Neuron-specific conditional expression of a mitochondrially targeted fluorescent protein in mice. J Neurosci 26(51):13123-7. [PubMed: 17182763]  [J:118452]


Price and Supply Information

Strain Name: C57BL/6-Tg(tetO-COX8A/EYFP)1Ksn/J
Stock Number: 006618

Price Details

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Supply Details

Standard SupplyRepository-Live. No age specifications accepted. Colony sized to produce minimal quantities (typically up to 6 mice) upon order receipt; one order per strain. Expected delivery: 1-3 months. Larger quantities or custom orders arranged upon request.
Supply Notes Usually shipped between four and eight weeks of age.
This strain is included in the Induced Mutant Resource Colony collection.
LicensingSee General Terms and Conditions below for Licensing and Use Restrictions  
Control InformationView Control Information in Strain Details.

General Terms and Conditions

View JAX® Mice & Services Conditions of Use.

Use of the Tet-System may require a license, see Licenses for Strains Using TET-System Technology.

For additional Licensing and Use Restrictions view the link(s) below:
- Strain(s) not available to companies or for-profit entities.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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