Strain Name: |
C57BL/6-Tg(tetO-COX8A/EYFP)1Ksn/J |
|---|---|
Stock Number: |
006618 |
Availability: | Repository- Live |
General Terms and Conditions |
| Former Name |
C57BL6-Tg(tetO-COX8A/EYFP)1Ksn/J (Changed: 22-MAR-07
) |
|
C57BL6-Tg(TRE-COX8/EYFP)001Ksn/J (Changed: 13-MAR-07
) | |
| Genes & Alleles | COX8A; YFP; Tg(tetO-COX8A/EYFP)1Ksn; tetO; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Coisogenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Mating System +/+ sibling x Hemizygote (Female x Male) Species laboratory mouse Donating Investigator Tibor Kristian, University of Maryland Generation N7+ (09-JUL-07) Strain Description
Hemizygotes are viable and fertile. These transgenic mice express a mitochondrial-specific EYFP fusion protein under the control of a tetracycline-responsive promoter element (TRE;tetO). When hemizygotes are bred with another transgenic mouse expressing either reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA) under the regulation of tissue-specific promoter, tissue-specific expression of EYFP in mitochondria of the bitransgenic offspring can be regulated with the tetracycline analog, doxycycline.When bred to a strain expressing rtTA or tTA in forebrain neurons (see Stock No. 003010 for example), this mutant mouse strain may be useful in studies of neuronal mitochondrial dysfunction.
Strain Development
A transgenic fusion protein construct containing the mitochondrial targeting sequence from the human COX8 gene and Enhanced Yellow Fluorescent Protein (EYFP) was placed downstream of a tet-responsive promoter element (TRE) and upstream of a beta-globin poly A signal. The construct was injected into fertilized C57BL/6 eggs. Founder animals were bred to C57BL/6 for six generations by the donating investigator.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Tg(tetO-COX8A/EYFP)1Ksn | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Tibor Kristian | ||
| Common Name(s) | TRE-mito/EYFP; pTRE2-mito/eYFP; | ||
| Mutation Made By | Tibor Kristian, University of Maryland | ||
| Strain of Origin | C57BL/6 | ||
| Site of Expression | mitochondrial-specific EYFP fusion protein under the control of a tetracycline-responsive promoter element (TRE or tetO) | ||
| Expressed Gene | YFP, Yellow Fluorescent Protein, jellyfish | ||
| Yellow Fluorescent Protein (YFP) is a derivative of Green Fluorescent Protein (GFP), a versatile reporter molecule which has found use in many biological applications. The original molecule has been modified in order to enhance fluorescence intensity and shift the wavelength emitted when excited. When YFP is utilized in a transgenic construct, tissue expressing sufficient amounts of YFP will fluoresce yellowish-green when exposed to a 513 nm light source. | |||
| Expressed Gene | COX8A, cytochrome c oxidase subunit 8A (ubiquitous), human | ||
| Promoter | tetO, tet operator, | ||
| Molecular Note | A construct encoding a fusion protein comprising the mitochondrial targeting sequence from human cytochrome c oxidase subunit 8A (ubiquitous) joined to enhanced yellow fluorescent protein (EYFP) was placed downstream of the tetracycline responsive element (tetO) and upsteam of a beta-globin polyadenylation signal. A mouse with this transgene can be bred with another transgenic mouse expressing reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA) under control of a selected promoter to produce bitransgenic mice in which mitochondrial EYFP expression can be regulated in a tissue-specific manner by administration/removal of the tetracycline analog, doxycycline. [J:118452] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Colony Maintenance
| Diet Information | LabDiet® 5K52/5K67 |
|---|
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (135 strains)
007940 B6.Cg-Tg(Thy1-CFP/COX8A)C1Lich/J 006614 B6;CB-Tg(Thy1-CFP/COX8A)C1Lich/J 006617 B6;CB-Tg(Thy1-CFP/COX8A)S2Lich/J
005483 129-Tg(CAG-EYFP)7AC5Nagy/J 006148 B6.129X1-Gt(ROSA)26Sortm1(EYFP)Cos/J 007901 B6.Cg-Tg(Thy1-Brainbow1.0)HLich/J 007911 B6.Cg-Tg(Thy1-Brainbow1.1)MLich/J 007921 B6.Cg-Tg(Thy1-Brainbow2.1)RLich/J 007612 B6.Cg-Tg(Thy1-COP4/EYFP)18Gfng/J 007615 B6.Cg-Tg(Thy1-COP4/EYFP)9Gfng/J 005630 B6.Cg-Tg(Thy1-EYFP)15Jrs/J 003709 B6.Cg-Tg(Thy1-YFP)16Jrs/J 005627 B6.Cg-Tg(Thy1-YFP/Syp)10Jrs/J 003782 B6.Cg-Tg(Thy1-YFPH)2Jrs/J 007606 B6.Cg-Tg(Thy1-cre/ESR1,-EYFP)AGfng/J 007610 B6.Cg-Tg(Thy1-cre/ESR1,-EYFP)VGfng/J 007910 B6;CBA-Tg(Thy1-Brainbow1.0)LLich/J 005620 B6;D2-Tg(S100B-EYFP)1Wjt/J 007880 B6SJL-Tg(Thy1-Stx1a/EYFP)1Sud/J 006362 C57BL/6J-Tg(CMV-Cox8a/EYFP)17J/J 005130 STOCK Gt(ROSA)26Sortm1(Smo/EYFP)Amc/J
006361 B6.Cg-Tg(Sp7-tTA,tetO-EGFP/cre)1Amc/J 003762 B6.Cg-Tg(tetFosb)4468Nes/J 007051 B6.Cg-Tg(tetO-APPSwInd)102Dbo/J 007052 B6.Cg-Tg(tetO-APPSwInd)107Dbo/J 007049 B6.Cg-Tg(tetO-APPSwInd)885Dbo/J 007618 B6.Cg-Tg(tetO-Arntl)1Jt/J 006234 B6.Cg-Tg(tetO-cre)1Jaw/J 005738 B6.FVB-Tg(tetO-EGFP,-Tgfbr2)8Mcle/J 002709 B6;C3-Tg(TettTALuc)1Dgs/J 008082 B6;SJL-Tg(Tagln-tTA)1Mrab Tg(tetO-Mcpt1)1Mrab/J 002621 B6;SJL-Tg(tetop-lacZ)2Mam/J 006004 B6C3-Tg(tetO-APPSwInd)885Dbo/J 006244 C.Cg-Tg(tetO-cre)1Jaw/J 005706 C57BL/6-Tg(tetO-CDK5R1/GFP)337Lht/J 006439 FVB-Tg(tetO/CMV-KRAS*G12C)9.1Msmi/J 005941 FVB/N-Tg(tetO-Aurkb,lacZ)41Kra/J 006202 FVB/N-Tg(tetO-BCR/ABL1)2Dgt/J 003315 FVB/N-Tg(tetORo1-lacZ)3Conk/J 005076 NOD.Cg-Tg(tetO-EGFP/FADD)1Doi/DoiJ 006999 STOCK Dbttm1Geh Tg(tTALap)5Bjd Tg(tetO-DBT)A1Geh/J 008168 STOCK Tg(tetO-DTA)1Gfi/J 005104 STOCK Tg(tetO-HIST1H2BJ/GFP)47Efu/J 005699 STOCK Tg(tetO-Ipf1,EGFP)956.6Macd/J 005728 STOCK Tg(tetO-Ipf1,lacZ)958.1Macd/J 006224 STOCK Tg(tetO-cre)1Jaw/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Tet Expression Systems
Visit the Alzheimer's Disease Mouse Model Resource site for helpful information on Alzheimer's Disease and research resources.
Animal Health Reports
Room Number AX11
Research Applications
This mouse can be used to support research in many areas including:
YFP relatedNeurobiology Research
Alzheimer's Disease
Fluorescent protein expression in neural tissue
Parkinson's Disease
Tet Expression System (tTA/rtTA Responsive Strains)
Research Tools
Fluorescent Proteins
Tet Expression Systems (tTA/rtTA Responsive Strains)
Research Tools
Fluorescent Proteins
References
Selected Reference(s)
Chandrasekaran K; Hazelton JL; Wang Y; Fiskum G; Kristian T. 2006. Neuron-specific conditional expression of a mitochondrially targeted fluorescent protein in mice. J Neurosci 26(51):13123-7. [PubMed: 17182763] [J:118452]
Price and Supply Information
| Strain Name: | C57BL/6-Tg(tetO-COX8A/EYFP)1Ksn/J |
| Stock Number: | 006618 |
Price Details
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Supply Details
| Standard Supply | Repository-Live. No age specifications accepted. Colony sized to produce minimal quantities (typically up to 6 mice) upon order receipt; one order per strain. Expected delivery: 1-3 months. Larger quantities or custom orders arranged upon request. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Use of the Tet-System may require a license, see Licenses for Strains Using TET-System Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Strain(s) not available to companies or for-profit entities.
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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