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Genes & Alleles

Ccr7;   Ccr7tm1Rfor;

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Product Information

Strain Details

Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Mating System Homozygote x Homozygote         (Female x Male) Species laboratory mouse Donating Investigator Martin Lipp,   Max-Delbrueck-Center Generation F9+F2 (14-MAY-08)

Strain Description
Homozygous mice are viable and fertile and show delayed primary B or T cell immune responses. Lymph nodes from homozygous mice are devoid of naive T cells and dendritic cells (DCs), but the T cell populations in the blood, the red pulp of the spleen, and in the bone marrow are greatly expanded. Secondary lymph organs exhibit morphological abnormalities, and adoptive transfer experiments demonstrate impaired B- and T-cell migration. In a model of acute allogeneic tumor rejection, homozygous mice fail to reject subcutaneously injected MHC class I mismatched tumor cells, and cytotoxic activity of allospecific T cells is severely compromised. These mutant mice (along with CXCR5-deficient mice - Stock No. 006659) - may be useful in immunological studies of chemokine receptors, including T- and B-cell function in primary and adaptive immune responses, entry of lymphocytes and dendritic cells into secondary lymphoid organs (and their homing to T- and B-cell zones therein), alloimmune responses, and the development of transplant rejection.

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.

Strain Development
A targeting vector was designed to replace a fragment of the third exon of the targeted gene (encompassing amino acids Ser-139 to Asp-309) with a neomycin resistance gene. The construct was electroporated into 129P2/OlaHsd-derived E14K embryonic stem (ES) cells, and correctly targeted ES cells were injected into BALB/c blastocysts. Chimeric males were bred to BALB/c. Mutant mice were backcrossed to C57BL/6 mice for 8 generations prior to arrival at The Jackson Laboratory.

Mammalian Phenotype Terms assigned by genotype The following phenotype information may relate to a genetic background differing from this JAX® Mice strain. Ccr7tm1Rfor/Ccr7tm1Rfor         involves: 129P2/OlaHsd * BALB/c hematopoietic system phenotype abnormal CD4-positive T cell morphology (MGI Ref ID J:57976) 6-fold increase in CD4+ cells in peripheral blood, spleen, and bone marrow decreased numbers of CD4+ cells in mesenteric and peripheral lymph nodes enlarged spleen (MGI Ref ID J:57976) 2- to 3-fold enlarged spleen immune system phenotype abnormal CD4-positive T cell morphology (MGI Ref ID J:57976) 6-fold increase in CD4+ cells in peripheral blood, spleen, and bone marrow decreased numbers of CD4+ cells in mesenteric and peripheral lymph nodes abnormal T cell physiology (MGI Ref ID J:57976) impaired T cell migration into lymph nodes inability to mount primary T cell response, assessed by lack of contact sensitivity reaction and delayed type sensitivity reaction abnormal antigen presenting cell physiology (MGI Ref ID J:57976) impaired dendritic cell migration into lymph nodes abnormal B cell physiology (MGI Ref ID J:57976) impaired B cell migration into lymph nodes decreased immunoglobulin level (MGI Ref ID J:57976) decreased IgG3 increased immunoglobulin level (MGI Ref ID J:57976) increased IgG2a, IgG1, IgE abnormal lymph node cortex (MGI Ref ID J:57976) abnormal distribution of B and T cells some enlarged germinal centers in B cell follicles enlarged spleen (MGI Ref ID J:57976) 2- to 3-fold enlarged spleen small Peyer's patches (MGI Ref ID J:57976) small lymph nodes (MGI Ref ID J:57976)

Gene & Allele Details

Allele Symbol		Ccr7tm1Rfor
Allele Name		targeted mutation 1, Reinhold Forster
Common Name(s)		CCR7-;
Mutation Made By		Martin Lipp,   Max-Delbrueck-Center
Strain of Origin		129P2/OlaHsd
ES Cell Line Name		E14K
ES Cell Line Strain		129P2/OlaHsd
Gene Symbol and Name		Ccr7, chemokine (C-C motif) receptor 7
Chromosome		11
Gene Common Name(s)		BLR2; CD197; CDw197; CMKBR7; Cmkbr7; EBI1; Ebi1h; Epstein-Barr virus induced gene 1 homolog; MGC108519; chemokine (C-C) receptor 7;
Molecular Note		0.5 kb of exon 3 encoding serine 139 to aspartic acid 309 was disrupted by insertion of a neomycin resistance cassette via homologous recombination. Successful targeting was verified in homozygous mutant animals by Southern blot analysis and chemotaxis assays. Spleen cells from homozygous animals demonstrated lack of chemotactic response towards EBI-1 ligand chemokine (ELC), a known ligand of the targeted gene. [MGI Ref ID J:57976]

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls

Genotyping Protocols

Ccr7^tm1Rfor

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, homozygous mice are bred.
Diet Information	LabDiet® 5K52/5K67

Related Strains

Strains carrying other alleles of Ccr7

005794

B6.129P2-Ccr7tm1Dgen/J

View Strains carrying other alleles of Ccr7     (1 strain)

Additional Web Information

Congenic Nomenclature

Animal Health Reports

Room Number           AX11

Research Applications

This mouse can be used to support research in many areas including:

Hematological Research
Immunological Defects (B and T cell deficiency)

Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers (genes regulating susceptibility to infectious disease and endotoxin)
Growth Factors/Receptors/Cytokines
Immunodeficiency (B and T cell deficiency)
Immunodeficiency (B cell defects)
Immunodeficiency (B cell deficiency)
Immunodeficiency (T cell deficiency)
Immunodeficiency (defects in humoral immune responses)
Immunodeficiency (multiple immune defects)
Inflammation (B and T cell deficiency)
Lymphoid Tissue Defects (B and T cell deficiency)
Lymphoid Tissue Defects (Lymphocyte Homing)

Internal/Organ Research
Lymphoid Tissue Defects (B and T cell deficiency)
Lymphoid Tissue Defects (T cell deficiency)

Research Tools
Immunology and Inflammation Research (B and T cell deficiency)
Immunology and Inflammation Research (B cell deficiency)
Immunology and Inflammation Research (T cell deficiency)
Immunology and Inflammation Research (genes regulating susceptibility to infectious disease and endotoxin)

References

Selected Reference(s)

Forster R; Schubel A; Breitfeld D; Kremmer E; Renner-Muller I; Wolf E; Lipp M. 1999. CCR7 coordinates the primary immune response by establishing functional microenvironments in secondary lymphoid organs. Cell 99(1):23-33. [PubMed: 10520991]  [MGI Ref ID J:57976]

Hopken UE; Droese J; Li JP; Joergensen J; Breitfeld D; Zerwes HG; Lipp M. 2004. The chemokine receptor CCR7 controls lymph node-dependent cytotoxic T cell priming in alloimmune responses. Eur J Immunol 34(2):461-70. [PubMed: 14768051]  [MGI Ref ID J:121522]

Additional References

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Price and Supply Information

Strain Name:	B6.129P2(C)-Ccr7tm1Rfor/J
Stock Number:	006621

Price Details

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Supply Details

Standard Supply	Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes	Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection.
Licensing	See General Terms and Conditions below for Licensing and Use Restrictions
Control Information	View Control Information in Strain Details.

General Terms and Conditions

View JAX^® Mice & Services Conditions of Use.

For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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