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- Description
- Phenotype
- Genes & alleles
- Genotyping
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- References
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Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Donating Investigator Peter Mombaerts, The Rockefeller University Development
The Olfr151tm9Mom mutation was generated in 129P2/OlaHsd derived E14 ES cells. To excise the neomycin resistance sequence of the LNL cassette, a resulting mouse was bred with a mouse carrying Tg(EIIa-cre)Lmgd, a transgene generated in FVB/N but having been backcrossed at least 10 times to C57BL/6J before this cross. The transgene was then bred out of the line and the mice were intercrossed to homozygosity. In 2007 sperm was cryopreserved from homozygous males on this predominantly mixed B6;129P2 background.The plasmid that was used to generate this mutation is banked and described at Addgene.
Related Strains
Strains carrying other alleles of Olfr151
View Strains carrying other alleles of Olfr151 (29 strains)
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Olfr151tm9Mom | ||
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| Allele Name | targeted mutation 9, Peter Mombaerts | ||
| Allele Type | Targeted (knock-in) | ||
| Common Name(s) | Hyb I; M71-M72 hybrid I; | ||
| Strain of Origin | 129P2/OlaHsd | ||
| ES Cell Line Name | E14 | ||
| ES Cell Line Strain | 129P2/OlaHsd | ||
| Gene Symbol and Name | Olfr151, olfactory receptor 151 | ||
| Chromosome | 9 | ||
| Gene Common Name(s) | GA_x6K02T2PVTD-31408136-31407207; M71; MOR171-2; | ||
| Molecular Note | One bp change resulting in 1 amino acid substitutions (A157T) and an IRES-tauGFP gene were introduced via homologous recombination. The resulting protein is a hybrid between Olfr151 and Olfr160 which have a total of 25 bp and 11 amino acid differences. The GFP gene was fused after the stop codon and co-expressed with the mutant protein. [MGI Ref ID J:90716] | ||
Genotyping
Genotyping Information
This strain will not have a genotyping protocol or one is not currently available.
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Feinstein P; Mombaerts P. 2004. A contextual model for axonal sorting into glomeruli in the mouse olfactory system. Cell 117(6):817-31. [PubMed: 15186781] [MGI Ref ID J:90716]
Health & husbandry
Health & Colony Maintenance Information
Currently there no information available for this strain. This may be due to the supply level of this strain.
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Animals Provided
Price (US dollars $) Cryorecovery Fee $1900.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
| Pricing for International shipping destinations |
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Animals Provided
Price (US dollars $) Cryorecovery Fee $2470.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
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Supply Details
| Standard Supply | Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
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Payment Terms and Conditions
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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