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- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Former Names STOCK Olfr160tm1Mom/MomJ (Changed: 01-APR-10 ) Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Donating Investigator Peter Mombaerts, Max Planck Institute of Biophysics Development
This targeted mutation was generated in 129P2/OlaHsd derived E14 ES cells and the resulting mice from clone U526 were crossed to Tg(EIIa-cre) bearing mice to excise the loxP flanked pgkneo (LNL) cassette. Although this transgene was generated in FVB/N mice, it was backcrossed onto C57BL/6J prior to this cross. The offspring were intercrossed and the transgene was selectively bred out of this line. Thus, this STOCK background is predominantly C57BL/6J and 129P2/OlaHsd. In 2007 sperm were cryopreserved from homozygous males.The plasmid that was used to generate this mutation is banked and described at Addgene.
Related Strains
Strains carrying Olfr160tm1(Olfr151)Mom allele
006740 STOCK Olfr160tm1(Olfr151)Mom Tg(Olfr151,taulacZ)AMom/MomJ 006741 STOCK Olfr160tm1(Olfr151)Mom Tg(Olfr151,taulacZ)BMom/MomJ View Strains carrying Olfr160tm1(Olfr151)Mom (2 strains)
007766 B6.129P2(Cg)-Olfr160tm6Mom/MomJ 006714 B6;129P2-Olfr160tm11(Olfr545)Mom/MomJ 006596 B6;129P2-Olfr160tm4Mom/MomJ 006687 B6;129P2-Olfr160tm7Mom/MomJ 006715 B6;129P2-Olfr545tm3(Olfr160)Mom/MomJ 007974 B6;CBA-Tg(Olfr160-taulacZ)V4-7Mom/MomJ 021206 STOCK Olfr160tm1.1(COP4*/EYFP)Tboz/J 006652 STOCK Olfr160tm2Mom/MomJ 006696 STOCK Olfr160tm3Mom/MomJ 006636 STOCK Olfr160tm5(Cnga2)Mom/MomJ 006678 STOCK Olfr160tm6Mom/MomJ
Phenotype
Phenotype Information
Currently there is no phenotype information for this strain.This mouse can be used to support research in many areas including:GFP related
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Olfr160tm1(Olfr151)Mom | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Peter Mombaerts | ||
| Allele Type | Targeted (knock-in) | ||
| Common Name(s) | M71->M72-GFP; M71->M72-IRES-tauGFP; M71-M72; Olfr160tm1Mom; | ||
| Mutation Made By | Peter Mombaerts, Max Planck Institute of Biophysics | ||
| Strain of Origin | 129P2/OlaHsd | ||
| ES Cell Line Name | E14 | ||
| ES Cell Line Strain | 129P2/OlaHsd | ||
| Site of Expression | GFP is expressed in olfactory sensory neurons. | ||
| Expressed Gene | Olfr151, olfactory receptor 151, mouse, laboratory | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Molecular Note | The entire coding region of Olfr160 was replaced with Olfr151 and an IRES-tauGFP gene introduced via homologous recombination. [MGI Ref ID J:90716] | ||
| Gene Symbol and Name | Olfr160, olfactory receptor 160 | ||
| Chromosome | 9 | ||
| Gene Common Name(s) | GA_x6K02T2PVTD-31389446-31388517; M72; MOR171-3; OR11-318; OST025; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Olfr160tm1Mom, Separated PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Feinstein P; Mombaerts P. 2004. A contextual model for axonal sorting into glomeruli in the mouse olfactory system. Cell 117(6):817-31. [PubMed: 15186781] [MGI Ref ID J:90716]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1980.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2574.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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JAX® Mice, Products & Services Conditions of Use
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