| |||||||||||||||||||
| These dopamine transporter IRES-cre (DATIREScre) mutant mice exhibit co-localization of Cre recombinase and endogenous Slc6a3 (solute carrier family 6 (neurotransmitter transporter, dopamine), member 3) gene expression and may be useful in neurobiological studies to facilitate the analysis of gene function in dopaminergic neurons, such as drug addiction or Parkinson's disease. | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
Strain Information
Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System +/+ sibling x Heterozygote (Female x Male) 16-DEC-08 Species laboratory mouse Generation N10F6 (03-DEC-12)
Generation DefinitionsDonating Investigator Cristina M Backman, National Institute on Drug Abuse (NIH) Description
Mice homozygous for this dopamine transporter IRES-cre (DATIREScre) mutant allele are viable and fertile. Cre recombinase activity is observed as early as embryonic day 15, and co-localizes with endogenous gene expression in adult dopaminergic cell groups (substantia nigra (SN) and ventral tegmental area (VTA), as well as in the retrorubral field). Lesser Cre recombinase activity occurs in adult olfactory bulb glomeruli, mimicking the known lower Slc6a3 (or DAT) expression in this tissue. Although the pattern and intensity of DAT immunostaining in the SN, VTA and striatum do not differ between wild-type and mutant mice, striatum DAT protein levels are moderately reduced (17%) in heterozygotes and significantly reduced (47%) in homozygotes. This diminution in homozygous striatum is associated with significantly increased neuropeptide PDyn (but not D1, D2, or PPE) mRNA levels compared to wild-type, while such an increase is not observed in heterozygotes. When bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the flanked genome in dopaminergic neurons. As such, these mutant mice may be useful in neurobiological studies to facilitate the analysis of gene function in dopaminergic neurons, such as drug addiction or Parkinson's disease.In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. The strain description will be modified as published results become available.
Development
To co-express both genes from the endogenous promoter (and minimize interference with endogenous gene function), a targeting vector was designed to insert an internal ribosome entry site-linked Cre recombinase gene (IRES-Cre) and FRT-flanked PGK-neo cassette 23 bp downstream from the stop codon of the endogenous gene. The construct was electroporated into C57BL/6-derived CMT2 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and chimeric mice were bred to C57BL/6 mice. The FRT-flanked neo cassette was removed in the germline mice by intercrossing with a Flpe-deleter strain on a mixed B6;SJL background (see Stock No. 003800). The resulting mutant offspring (now harboring this DATIREScre allele) were maintained on this mixed genetic background prior to arrival at The Jackson Laboratory. Upon arrival, mice were backcrossed to the C57BL/6J inbred strain for at least five generations.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Parkinson's Disease Models
View Parkinson's Disease Models (109 strains)
016583 C57BL/6N-Tg(Slc6a3-icre/ERT2)2Gloss/J 009090 FVB/NJ-Tg(Slc6a3-PARK2*Q311X)AXwy/J
Additional Web Information
Introduction to Cre-lox technology
Visit the Parkinson's Disease Resource site for helpful information on Parkinson's and research resources.
Phenotype
Phenotype Information
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested. Attention Deficit-Hyperactivity Disorder; ADHD (SLC6A3)
Major Affective Disorder 1; MAFD1 (SLC6A3)
Parkinsonism-Dystonia, Infantile; PKDYS (SLC6A3)
Tobacco Addiction, Susceptibility to (SLC6A3)
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Slc6a3tm1.1(cre)Bkmn/Slc6a3tm1.1(cre)Bkmn
involves: 129S1/Sv * 129X1/SvJ * C57BL/6
- normal phenotype
- no abnormal phenotype detected
- homozygous and heterozygous mice are normal and viable (MGI Ref ID J:114466)
This mouse can be used to support research in many areas including:
cre relatedCell Biology Research
Channel and Transporter Defects
Neurobiology Research
Behavioral and Learning Defects
Channel and Transporter Defects
Neurotransmitter Receptor and Synaptic Vesicle Defects
Research Tools
Cre-lox System
Cre Recombinase Expression
Cre Recombinase Expression: Germline/Embryonic Expression
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Slc6a3tm1.1(cre)Bkmn relatedResearch Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Neurobiology Research
Parkinson's Disease
strains expressing cre
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Slc6a3tm1.1(cre)Bkmn | ||
|---|---|---|---|
| Allele Name | targeted mutation 1.1, Cristina M Backman | ||
| Allele Type | Targeted (knock-in) | ||
| Common Name(s) | DatIREScre; | ||
| Mutation Made By | Andreas Tomac, National Institute on Drug Abuse (NIH) | ||
| ES Cell Line Name | Other (see notes) | ||
| Site of Expression | Cre recombinase activity is observed as early as embryonic day 15, and co-localizes with endogenous gene expression in adult dopaminergic cell groups (substantia nigra (SN) and ventral tegmental area (VTA), as well as in the retrorubral field). Lesser Cre recombinase activity occurs in adult olfactory bulb glomeruli, mimicking the known lower Slc6a3 (or DAT) expression in this tissue. | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Driver Note | Slc6a3 | ||
| General Note | The targeting vector was electroporated into both R1 and C57BL/6 ES cells. | ||
| Molecular Note | An FRT-flanked neo was removed from the locus, leaving cre recombinase cDNA in the 3' UTR to allow bicistronic mRNA expression. [MGI Ref ID J:114466] | ||
| Gene Symbol and Name | Slc6a3, solute carrier family 6 (neurotransmitter transporter, dopamine), member 3 | ||
| Chromosome | 13 | ||
| Gene Common Name(s) | DAT; DAT1; Dat1; PKDYS; dopamine transporter 1; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Slc6a3tm1.1(cre)Bkmn, Melt Curve Analysis
Slc6a3tm1.1(cre)Bkmn, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Backman CM; Malik N; Zhang Y; Shan L; Grinberg A; Hoffer BJ; Westphal H; Tomac AC. 2006. Characterization of a mouse strain expressing Cre recombinase from the 3' untranslated region of the dopamine transporter locus. Genesis 44(8):383-90. [PubMed: 16865686] [MGI Ref ID J:114466]
Additional References
Slc6a3tm1.1(cre)Bkmn relatedBello EP; Mateo Y; Gelman DM; Noain D; Shin JH; Low MJ; Alvarez VA; Lovinger DM; Rubinstein M. 2011. Cocaine supersensitivity and enhanced motivation for reward in mice lacking dopamine D2 autoreceptors. Nat Neurosci 14(8):1033-8. [PubMed: 21743470] [MGI Ref ID J:176167]
Cohen JY; Haesler S; Vong L; Lowell BB; Uchida N. 2012. Neuron-type-specific signals for reward and punishment in the ventral tegmental area. Nature 482(7383):85-8. [PubMed: 22258508] [MGI Ref ID J:181307]
Di Salvio M; Di Giovannantonio LG; Acampora D; Prosperi R; Omodei D; Prakash N; Wurst W; Simeone A. 2010. Otx2 controls neuron subtype identity in ventral tegmental area and antagonizes vulnerability to MPTP. Nat Neurosci 13(12):1481-8. [PubMed: 21057506] [MGI Ref ID J:166896]
Diaz-Ruiz O; Zapata A; Shan L; Zhang Y; Tomac AC; Malik N; de la Cruz F; Backman CM. 2009. Selective deletion of PTEN in dopamine neurons leads to trophic effects and adaptation of striatal medium spiny projecting neurons. PLoS One 4(9):e7027. [PubMed: 19750226] [MGI Ref ID J:153622]
Genetic Resource Sciences at The Jackson Laboratory. 2013. Expression/Specificity Patterns of Cre Alleles, 2013 MGI Direct Data Submission :. [MGI Ref ID J:193672]
Pham AH; Meng S; Chu QN; Chan DC. 2012. Loss of Mfn2 results in progressive, retrograde degeneration of dopaminergic neurons in the nigrostriatal circuit. Hum Mol Genet 21(22):4817-26. [PubMed: 22859504] [MGI Ref ID J:188347]
Ziegler CG; Peng M; Falk MJ; Polyak E; Tsika E; Ischiropoulos H; Bakalar D; Blendy JA; Gasser DL. 2012. Parkinson's disease-like neuromuscular defects occur in prenyl diphosphate synthase subunit 2 (Pdss2) mutant mice. Mitochondrion 12(2):248-57. [PubMed: 21983691] [MGI Ref ID J:192142]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, heterozygous mice are bred to wildtype siblings or to inbred C57BL/6J (Stock No. 000664). Homozygous mice have approximately half of the endogenous gene expression associated with altered neuropeptide levels in the brain, while heterozygotes have a moderate, non-significant reduction in endogenous gene expression with no reported neuropeptide abnormalities. Mating System +/+ sibling x Heterozygote (Female x Male) 16-DEC-08 Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $232.00 Female or Male Heterozygous for Slc6a3tm1.1(cre)Bkmn
Price per Pair (US dollars $) Pair Genotype $296.00 Heterozygous for Slc6a3tm1.1(cre)Bkmn x Wild-type for Slc6a3tm1.1(cre)Bkmn $296.00 Wild-type for Slc6a3tm1.1(cre)Bkmn x Heterozygous for Slc6a3tm1.1(cre)Bkmn Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $301.60 Female or Male Heterozygous for Slc6a3tm1.1(cre)Bkmn
Price per Pair (US dollars $) Pair Genotype $384.80 Heterozygous for Slc6a3tm1.1(cre)Bkmn x Wild-type for Slc6a3tm1.1(cre)Bkmn $384.80 Wild-type for Slc6a3tm1.1(cre)Bkmn x Heterozygous for Slc6a3tm1.1(cre)Bkmn Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
|
|
|
Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form
Terms of Use
Terms of Use
General Terms and Conditions
For Licensing and Use Restrictions view the link(s) below:
- Strain(s) not available to companies or for-profit entities.
- Strain(s) not available to companies or for-profit entities.
Contact information
General inquiries regarding Terms of UseContracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.
No Liability
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.