Strain Name: |
129S.B6-Tg(KRT14-RAF1/ESR1)1Pkha/J |
|---|---|
Stock Number: |
006661 |
Availability: | Repository- Live |
General Terms and Conditions |
| Former Name |
129.B6-Tg(KRT14-RAF1/Esr1)1Pkha/J (Changed: 13-APR-07
) |
| Genes & Alleles | ESR1; KRT14; RAF1; Tg(KRT14-RAF1/ESR1)1Pkha; |
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Mating System +/+ sibling x Hemizygote (Female x Male) Species laboratory mouse Donating Investigator Paul Khavari, Stanford University Generation N6+N1F1 (03-DEC-07) Strain Description
Mice hemizygous for this "K14-Raf:ER" transgene are viable and fertile. Inducible expression of the human Raf1(Raf-1 [DD]):estrogen receptor (ER) fusion protein is observed in the epidermis following topical application of 4-hydroxytamoxifen (4OHT). Prolonged (4 weeks) induction of human Raf-1[DD] activity promotes the undifferentiated, proliferative phenotypic characteristics observed in epidermal cancer; including hyperplasia, increased mitotic index, decreased expression of differentiation markers and increased expression of beta-1 and beta-4 integrin subunits. Raf-1[DD]-induced skin abnormalities are entirely reversed within one month after 4OHT cessation. These mice have a similar 4OHT-inducible skin phenotype as the transgenic mice expressing human H-RasG12V (Stock No. 006403) or human Mek1R4F (Stock No. 006822), and may be useful in studies of the Ras/Raf/MEK/ERK cell proliferation and differentiation pathway, as well as in elucidating cellular events preceding skin cancer cell transformation.Strain Development
The “K14-Raf:ER” transgene was designed with a 2075-bp human keratin 14 promoter sequence upstream of the constitutively active Y340D/Y341D mutant form of the catalytic domain of human Raf-1 (Raf-1[DD]) fused at its carboxy terminal with the hormone binding domain of the human estrogen receptor. This transgene was injected into the pronuclei of C57BL/6 embryos and chimeric founders were established. Mice were then backcrossed to “129/SvEv” mice for 5 generations prior to arrival at The Jackson Laboratory.
Mammalian Phenotype Terms assigned by genotype |
| Allele Symbol | Tg(KRT14-RAF1/ESR1)1Pkha | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Paul A Khavari | ||
| Common Name(s) | K14-Raf:ER; | ||
| Mutation Made By | Paul Khavari, Stanford University | ||
| Strain of Origin | C57BL/6 | ||
| Expressed Gene | RAF1, v-raf-1 murine leukemia viral oncogene homolog 1, human | ||
| Expressed Gene | ESR1, estrogen receptor 1, human | ||
| Promoter | KRT14, keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner), human | ||
| General Note | Three independent transgenic mouse lines were generated that exhibited similar levels of fusion protein expression. | ||
| Molecular Note | The transgene contains a 2075-bp human keratin 14 promoter construct driving expression of a cDNA that encodes a fusion protein comprising a constitutively active mutant form of the catalytic domain of human RAF1, in which tyrosine is replaced by aspartic acid at amino acid positions 340 and 341 (Y340D/Y341D), joined at its carboxyl terminal to the ligand-binding domain of human estrogen receptor 1. The fusion protein can be activated in the epidermis by topical application of 4-hydroxytamoxifen. [MGI Ref ID J:118584] [MGI Ref ID J:118585] [MGI Ref ID J:81660] | ||
| Control | ||
|---|---|---|
| Noncarrier | ||
| 002448 129S1/SvImJ | (approximate) | |
| Considerations for Choosing Controls | ||
Tg(KRT14-RAF1/ESR1)1Pkha
| Breeding & Husbandry | When maintaining a live colony, hemizygotes are bred to wildtype siblings or to inbred 129S1/SvImJ (Stock No. 002448) mice. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Strains carrying other alleles of ESR1
View Strains carrying other alleles of ESR1 (7 strains)
Strains carrying other alleles of KRT14
006403 129S.B6-Tg(KRT14-Esr1/HRAS)1Pkha/J 004853 B6.C3-Tg(KRT14-Birc5)19Gros/J 007622 B6;SJL-Tg(KRT14-Hr)551Cct/J 007678 B6;SJL-Tg(KRT14-rtTA)208Jek/J 002980 C57BL/6-Tg(Cd152Ig)1Jbs/J 006822 FVB-Tg(KRT14-MAP2K1/Esr1)12Pkha/J 005705 FVB-Tg(KRT14-Vegfa)3Dtm/J 008099 FVB-Tg(KRT14-rtTA)F42Efu/J 004782 STOCK Tg(KRT14-cre)1Amc/J 005107 STOCK Tg(KRT14-cre/Esr1)20Efu/J View Strains carrying other alleles of KRT14 (10 strains)
Congenic Nomenclature
Room Number AX11
Cancer Research
Genes Regulating Growth and Proliferation
Other
Cell Biology Research
Genes Regulating Growth and Proliferation
Signal Transduction
Dermatology Research
Other
Research Tools
Cancer Research
Cell Biology Research
Dermatology Research
Selected Reference(s)
Additional ReferencesTarutani M; Cai T; Dajee M; Khavari PA. 2003. Inducible activation of ras and raf in adult epidermis. Cancer Res 63(2):319-23. [PubMed: 12543782] [MGI Ref ID J:81660]
| Strain Name: | 129S.B6-Tg(KRT14-RAF1/ESR1)1Pkha/J |
| Stock Number: | 006661 |
IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
Purchasing Information
JAX® Mice Orders
Surgical Services
Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form