Strain Name: |
C57BL/6-Tg(ACTB-MAP2K1*K97M)1Stl/J |
|---|---|
Stock Number: |
006662 |
Availability: | Repository- Live |
General Terms and Conditions |
| Genes & Alleles | ACTB; MAP2K1; Tg(ACTB-MAP2K1*K97M)1Stl; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Coisogenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Mating System +/+ sibling x Hemizygote (Female x Male) Species laboratory mouse Donating Investigator Susumu Tonegawa, Massachusetts Institute of Technology Generation ?+F0 (28-DEC-07) Strain Description
Hemizygous mice are viable and fertile. These "dnMEK1" mice express a dominant-negative mutant (K97M) form of human MEK1 (synonym: MAP2K1) following Cre-mediated removal of the upstream "Lox-STOP-Lox" cassette; when transgenic mice are bred to a cre-expressing strain, the "floxed stop" cassete is excised in the resulting offspring, and mutant MEK1 expression is observed in the cre-expressing tissue(s). In the absence of Cre recombinase, transgene expression is not detectable in the brains of these "floxed" mice Because the MEK1 mutation abolishes the protein's kinase activity but preserves its ability to interact with ERK1 and ERK2, these transgenic mice may be useful in studying MEK-dependent activation and regulation of ERK, the ERK-MAPK signaling pathway, and neurological studies involving synaptic plasticity and memory.When bred to a strain expressing Cre recombinase in the CA1 pyramidal cell layer of the hippocampus (see Stock No. 005359 for example), this mutant mouse strain exhibits abnormal learning, memory and synaptic transmission.
Strain Development
A dominant-negative human MEK1 (or MAP2K1) cDNA bearing a K97M mutation (at the ATP binding site) and the SV40 late polyadenylation signal was inserted downstream of a chicken beta-actin-loxP-stop-loxP sequence. The transgene was injected into C57BL/6 embryos. The resulting transgenic mice were subsequently bred to C57BL/6 mice and maintained on that genetic background for many generations prior to arrival at The Jackson Laboratory.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Tg(ACTB-MAP2K1*K97M)1Stl | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Susumu Tonegawa | ||
| Common Name(s) | dnMEK1; | ||
| Strain of Origin | C57BL/6 | ||
| Site of Expression | Cre excision results in the removal of a stop codon, enabling kinase expression. | ||
| Expressed Gene | MAP2K1, mitogen-activated protein kinase kinase 1, human | ||
| Promoter | ACTB, actin, beta, chicken | ||
| Molecular Note | The transgene encodes a dominant-negative mutant (K97M) form of human MEK1 (or MAP2K1). Expression under the control of the chicken Actin beta promoter occurs only following Cre-mediated removal of the upstream Lox-STOP-Lox cassette. In the absence of Cre recombinase, the transgene is not detectably expressed in the brains of these floxed mice. If bred to a Cre-expressing strain, the floxed stop region is excised in the resulting offspring, and mutant MEK1 expression is observed in the cre-expressing tissue(s). [MGI Ref ID J:88546] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Tg(Sv40 splicing/polyadenylation)
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, hemizygotes are bred to wildtype siblings or to C57BL/6J inbred mice. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying other alleles of ACTB
View Strains carrying other alleles of ACTB (31 strains)
006822 FVB-Tg(KRT14-MAP2K1/Esr1)12Pkha/J
Additional Web Information
Cre-lox or FLP-FRT Systems
Animal Health Reports
Room Number AX11
Research Applications
This mouse can be used to support research in many areas including:
Cell Biology Research
Signal Transduction
Transcriptional Regulation
Neurobiology Research
Behavioral and Learning Defects
Cre-lox System (loxP-flanked Sequences)
Neurotransmitter Receptor and Synaptic Vesicle Defects
Research Tools
Cell Biology Research
Cre-lox System (loxP-flanked Sequences)
Genetics Research (Mutagenesis and Transgenesis: transcriptional activation)
References
Selected Reference(s)
Kelleher RJ rd; Govindarajan A; Jung HY; Kang H; Tonegawa S. 2004. Translational control by MAPK signaling in long-term synaptic plasticity and memory. Cell 116(3):467-79. [PubMed: 15016380] [MGI Ref ID J:88546]
Price and Supply Information
| Strain Name: | C57BL/6-Tg(ACTB-MAP2K1*K97M)1Stl/J |
| Stock Number: | 006662 |
Price Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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