Strain Name: |
B6.Cg-Tg(Eno2-cre)39Jme/J |
|---|---|
Stock Number: |
006663 |
Availability: | Repository- Live |
General Terms and Conditions |
| Genes & Alleles | Eno2; cre; Tg(Eno2-cre)39Jme; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Strain Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Transgenic Mating System Noncarrier x Hemizygote (Female x Male) Species laboratory mouse Donating Investigator IMR Colony, The Jackson Laboratory Generation N6 (29-APR-08) Strain Description
Mice hemizygous for this NSE39-Cre transgene are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. These NSE39-Cre mice harbor a transgenic insert consisting of the cre recombinase gene under the control of the promoter region of the rat neuron specific enolase (NSE or Eno2) gene. As such, Cre recombinase activity is directed to neurons with expression in many tissue types. When bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the flanked genome. Specifically, these NSE39-Cre transgenic mice may also be useful in studies of spinal muscular atrophy (SMA) along with mice harboring a conditional (floxed) Smn1 gene (see Stock No. 006138 or Stock No. 006146). Additional SMA strains expressing cre in striated muscle are available as well (see Stock No. 005936, Stock No. 006139, and Stock No. 006149).NSE39-Cre transgenic mice are available on different genetic backgrounds (see Stock No. 005938, Stock No. 006297, and Stock No. 006663). In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the NSE39-Cre phenotype could vary from that originally described on a mixed genetic background. We will modify the strain description if necessary as published results become available.
Importation of this model was supported by the Spinal Muscular Atrophy Foundation. Creation and development was supported by the National Institute of Health and Medical Research of France (Inserm) and the Association Française contre les Myopathies (AFM). An additional help was provided by Families of SMA (U.S.A.) and Andrew’s Buddies (U.S.A.).
Strain Development
A targeting vector was designed placing a cre recombinase gene (preceded by the rabbit beta-globin intron and followed by the SV40 polyadenylation signal) under control of the promoter region from the rat neuron specific enolase (NSE or Eno2) gene. This construct was microinjected into (C57BL6 x SJL)F1 embryos and implanted into pseudopregnant foster mothers. Founder 39 was bred to C57BL/6 to generate transgenic mice. At different points while maintaining this strain, transgenic mice were bred with C57BL/6 wildtype mice and/or mice harboring a loxP-flanked exon 7 mutation (Smn1tm1Jme or SMNF7) on a C57BL/6 and "129Sv" mixed background. As the donating investigator reports no germline expression for this transgene, Cre-mediated deletion of the “floxed” exon does not occur in the germline. Thus, offspring contained either the wildtype Smn1 locus or the “floxed” locus; never the Smn1 deletion. Transgenic offspring bearing the wildtype Smn1 locus on this mixed (but predominantly B6;129) background were sent to The Jackson Laboratory by Dr. Judith Melki in 2006. The mice were then backcrossed to C57BL/6J (Stock No. 000664) for 5 generations.
Gene & Allele Details
| Allele Symbol | Tg(Eno2-cre)39Jme | ||
|---|---|---|---|
| Allele Name | transgene insertion 39, Judith Melki | ||
| Common Name(s) | NSE39-Cre; Tg(Nse-cre)1Jme; Tg(Nse-cre)39Jme; | ||
| Mutation Made By | Judith Melki, Genopole, Inserm U798 | ||
| Strain of Origin | (C57BL/6J x SJL)F1 | ||
| Site of Expression | neurons in many tissue types | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Eno2, enolase 2, gamma, neuronal, rat | ||
| Molecular Note | This transgene expresses Cre recombinase under the control of a rat neuron-specific enolase promoter. [J:61396] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Smn1tm1Jme
Colony Maintenance
| Breeding & Husbandry | After arriving at The Jackson Laboratory on a mixed background, mutant mice were bred to wildtype C57BL/6J (Stock No. 000664) for 5-10 generations. The resulting backcrossed hemizygotes were maintained thereafter by breeding transgenic mice to either wildtype siblings from the colony or C57BL/6J. |
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| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying Tg(Eno2-cre)39Jme allele
006297 FVB.Cg-Tg(Eno2-cre)39Jme/J 005938 STOCK Tg(Eno2-cre)39Jme/J View Strains carrying Tg(Eno2-cre)39Jme (2 strains)
003833 B6.Cg-Tg(Eno2-Ighmpb2)17Cx Ighmbp2nmd-2J/Cx 003767 B6.Cg-Tg(Eno2tTA)5021Nes/J 003763 B6.Cg-Tg(Eno2tTA)5030Nes/J 004360 B6;SJL-Tg(HD)63Aron/J 003753 FVB/N-Tg(Eno2CDK5R1)1Jdm/J
Additional Web Information
Congenic Nomenclature
Cre-lox or FLP-FRT Systems
Animal Health Reports
Room Number AX11
Research Applications
This mouse can be used to support research in many areas including:
cre relatedDevelopmental Biology Research
Neurodevelopmental Defects
Neurobiology Research
Neurodevelopmental Defects
Neuromuscular Defects
Spinal Muscular Atrophy (SMA)
Research Tools
Cre-lox-System (Cre-Recombinase Expression: Germline/Embryonic Expression)
Genetics Research (Mutagenesis and Transgenesis: Cre-lox system)
Neurobiology Research
Research Tools
Cre-lox-System
Genetics Research (Mutagenesis and Transgenesis: Cre-lox system)
References
Selected Reference(s)
Additional ReferencesFrugier T; Tiziano FD; Cifuentes-Diaz C; Miniou P; Roblot N; Dierich A; Le Meur M; Melki J. 2000. Nuclear targeting defect of SMN lacking the C-terminus in a mouse model of spinal muscular atrophy. Hum Mol Genet 9(5):849-58. [PubMed: 10749994] [J:61396]
Price and Supply Information
| Strain Name: | B6.Cg-Tg(Eno2-cre)39Jme/J |
| Stock Number: | 006663 |
Price Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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