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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation N5pN1 Donating Investigator IMR Colony, The Jackson Laboratory Description
Mice hemizygous for this NSE39-Cre transgene are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. These NSE39-Cre mice harbor a transgenic insert consisting of the cre recombinase gene under the control of the promoter region of the rat neuron specific enolase (NSE or Eno2) gene. As such, Cre recombinase activity is directed to neurons with expression in many tissue types. When bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the flanked genome. Specifically, these NSE39-Cre transgenic mice may also be useful in studies of spinal muscular atrophy (SMA) along with mice harboring a conditional (floxed) Smn1 gene (see Stock No. 006138 or Stock No. 006146). Additional SMA strains expressing cre in striated muscle are available as well (see Stock No. 005936, Stock No. 006139, and Stock No. 006149).NSE39-Cre transgenic mice are available on different genetic backgrounds (see Stock No. 005938, Stock No. 006297, and Stock No. 006663). In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the NSE39-Cre phenotype could vary from that originally described on a mixed genetic background. We will modify the strain description if necessary as published results become available.
Importation of this model was supported by the Spinal Muscular Atrophy Foundation. Creation and development was supported by the National Institute of Health and Medical Research of France (Inserm) and the Association Française contre les Myopathies (AFM). An additional help was provided by Families of SMA (U.S.A.) and Andrew’s Buddies (U.S.A.).
Development
A targeting vector was designed placing a cre recombinase gene (preceded by the rabbit beta-globin intron and followed by the SV40 polyadenylation signal) under control of the promoter region from the rat neuron specific enolase (NSE or Eno2) gene. This construct was microinjected into (C57BL6 x SJL)F1 embryos and implanted into pseudopregnant foster mothers. Founder 39 was bred to C57BL/6 to generate transgenic mice. At different points while maintaining this strain, transgenic mice were bred with C57BL/6 wild type mice and/or mice harboring a loxP-flanked exon 7 mutation (Smn1tm1Jme or SMNF7) on a C57BL/6 and "129Sv" mixed background. As the donating investigator reports no germline expression for this transgene, Cre-mediated deletion of the ?floxed? exon does not occur in the germline. Thus, offspring contained either the wild type Smn1 locus or the ?floxed? locus; never the Smn1 deletion. Transgenic offspring bearing the wild type Smn1 locus on this mixed (but predominantly B6;129) background were sent to The Jackson Laboratory by Dr. Judith Melki in 2006. The mice were then backcrossed to C57BL/6J (Stock No. 000664) for five generations.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying Tg(Eno2-cre)39Jme allele
006297 FVB.Cg-Tg(Eno2-cre)39Jme/J 005938 STOCK Tg(Eno2-cre)39Jme/J View Strains carrying Tg(Eno2-cre)39Jme (2 strains)
003834 B6.Cg-Tg(Eno2-Ighmbp2)90Cx Ighmbp2nmd-2J/Cx 003833 B6.Cg-Tg(Eno2-Ighmpb2)17Cx Ighmbp2nmd-2J/Cx 003767 B6.Cg-Tg(Eno2tTA)5021Nes/J 003763 B6.Cg-Tg(Eno2tTA)5030Nes/J 004360 B6;SJL-Tg(HD)63Aron/J 007857 C57BL/6J-Tg(Eno2-YFP/Cox8a)YRwb/J 007860 C57BL/6J-Tg(Eno2-YFP/Cox8a)ZRwb/J 003753 FVB/N-Tg(Eno2CDK5R1)1Jdm/J
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
cre relatedDevelopmental Biology Research
Neurodevelopmental Defects
Neurobiology Research
Neurodevelopmental Defects
Neuromuscular Defects
Spinal Muscular Atrophy (SMA)
Research Tools
Cre-lox System
Cre Recombinase Expression: Germline/Embryonic Expression
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Neurobiology Research
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Eno2-cre)39Jme | ||
|---|---|---|---|
| Allele Name | transgene insertion 39, Judith Melki | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | NSE39-Cre; Tg(Nse-cre)1Jme; Tg(Nse-cre)39Jme; | ||
| Mutation Made By | Judith Melki, Genopole, Inserm U798 | ||
| Strain of Origin | (C57BL/6J x SJL)F1 | ||
| Site of Expression | neurons in many tissue types | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Eno2, enolase 2, gamma, neuronal, rat | ||
| Driver Note | Eno2 | ||
| Molecular Note | This transgene expresses Cre recombinase under the control of a rat neuron-specific enolase promoter. [MGI Ref ID J:61396] | ||
| Gene Symbol and Name | Tg(Eno2-cre)39Jme, transgene insertion 39, Judith Melki | ||
| Chromosome | UN | ||
| Gene Common Name(s) | NSE39-Cre; Tg(Nse-cre)1Jme; Tg(Nse-cre)39Jme; transgene insertion 1, Judith Melki; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic Cre Melt Curve Analysis, Melt Curve Analysis
Generic Cre, Standard PCR
Smn1tm1Jme, Standard PCR
Tg(Eno2-cre)39Jme, Melt Curve Analysis
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Frugier T; Tiziano FD; Cifuentes-Diaz C; Miniou P; Roblot N; Dierich A; Le Meur M; Melki J. 2000. Nuclear targeting defect of SMN lacking the C-terminus in a mouse model of spinal muscular atrophy. Hum Mol Genet 9(5):849-58. [PubMed: 10749994] [MGI Ref ID J:61396]
Additional References
Tg(Eno2-cre)39Jme relatedBuj-Bello A; Laugel V; Messaddeq N; Zahreddine H; Laporte J; Pellissier JF; Mandel JL. 2002. The lipid phosphatase myotubularin is essential for skeletal muscle maintenance but not for myogenesis in mice. Proc Natl Acad Sci U S A 99(23):15060-5. [PubMed: 12391329] [MGI Ref ID J:81791]
Kwon CH; Luikart BW; Powell CM; Zhou J; Matheny SA; Zhang W; Li Y; Baker SJ; Parada LF. 2006. Pten regulates neuronal arborization and social interaction in mice. Neuron 50(3):377-88. [PubMed: 16675393] [MGI Ref ID J:109635]
Puccio H; Simon D; Cossee M; Criqui-Filipe P; Tiziano F; Melki J; Hindelang C; Matyas R; Rustin P; Koenig M. 2001. Mouse models for Friedreich ataxia exhibit cardiomyopathy, sensory nerve defect and Fe-S enzyme deficiency followed by intramitochondrial iron deposits. Nat Genet 27(2):181-6. [PubMed: 11175786] [MGI Ref ID J:75420]
Zhou J ; Blundell J ; Ogawa S ; Kwon CH ; Zhang W ; Sinton C ; Powell CM ; Parada LF. 2009. Pharmacological inhibition of mTORC1 suppresses anatomical, cellular, and behavioral abnormalities in neural-specific Pten knock-out mice. J Neurosci 29(6):1773-83. [PubMed: 19211884] [MGI Ref ID J:146632]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Colony Maintenance
Breeding & Husbandry After arriving at The Jackson Laboratory on a mixed background, mutant mice were bred to wildtype C57BL/6J (Stock No. 000664) for 5-10 generations. The resulting backcrossed hemizygotes were maintained thereafter by breeding transgenic mice to either wildtype siblings from the colony or C57BL/6J.
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Animals Provided
Price (US dollars $) Cryorecovery Fee $1900.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
| Pricing for International shipping destinations |
|
Animals Provided
Price (US dollars $) Cryorecovery Fee $2470.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
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Supply Details
| Standard Supply | Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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