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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Former Names B6;129P2-Olfr151tm13(rI7)Mom/MomJ (Changed: 08-FEB-07 ) Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Donating Investigator Peter Mombaerts, Max Planck Institute of Biophysics Description
The coding region of the Olfr151 olfactory receptor was replaced by that of the rat I7 olfactory receptor. Olfactory sensory neurons that express the mutated locus co-express both GFP and taulacZ by virtue of IRES-mediated co-translation. These neurons can be revealed by the intrinsic fluorescence of GFP, by immunofluorescence with anti-GFP antibodies, by histochemistry of beta-galactosidase enzymatic activity, or by immunofluorescence with anti-beta-galactosidase antibodies. Expression of the inserted rat sequence instead of the endogenous sequence causes the location of axonal convergence in expressing neurons to be rerouted tod a more anterior, ventral position. These neurons respond to octanal.Development
The Olfr151tm13(rI7)Mom mutation was generated in 129P2/OlaHsd derived E14 ES cells. To excise the neomycin resistance sequence of the LNL cassette, a resulting mouse was bred with a mouse carrying Tg(EIIa-cre)Lmgd, a transgene generated in FVB/N but having been backcrossed at least 10 times to C57BL/6J before this cross. The transgene was then bred out of the line and the mice were intercrossed to homozygosity.The plasmid that was used to generate this mutation is banked and described at Addgene.
Related Strains
Strains carrying other alleles of Olfr151
View Strains carrying other alleles of Olfr151 (30 strains)
Phenotype
Phenotype Information
assigned by genotype
Olfr151tm13(Olr226)Mom/Olfr151tm13(Olr226)Mom
involves: 129P2/OlaHsd * C57BL/6
- nervous system phenotype
- *normal* nervous system phenotype
- glomeruli in the olfactory bulb form normally (MGI Ref ID J:91160)
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Olfr151tm13(Olr226)Mom | ||
|---|---|---|---|
| Allele Name | targeted mutation 13, Peter Mombaerts | ||
| Allele Type | Targeted (knock-in) | ||
| Common Name(s) | Olfr151tm13(rI7)Mom; rI7-M71-IRES-GFP-IRES-taulacZ; rI7-M71GFPiLacZ; | ||
| Strain of Origin | 129P2/OlaHsd | ||
| ES Cell Line Name | E14 | ||
| ES Cell Line Strain | 129P2/OlaHsd | ||
| Gene Symbol and Name | Olfr151, olfactory receptor 151 | ||
| Chromosome | 9 | ||
| Gene Common Name(s) | GA_x6K02T2PVTD-31408136-31407207; M71; MOR171-2; | ||
| Molecular Note | The coding region was replaced with the rat I7 OR followed by IRES-GFP-IRES-taulacZ. [MGI Ref ID J:91160] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Fluorescent Proteins (Generic GFP), Melt Curve Analysis
Fluorescent Proteins (Generic GFP), Standard PCR
Generic LacZ Melt Curve Analysis, Melt Curve Analysis
Generic LacZ, Standard PCR
Olfr151tm13(olr226)Mom, Separated PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Feinstein P; Bozza T; Rodriguez I; Vassalli A; Mombaerts P. 2004. Axon guidance of mouse olfactory sensory neurons by odorant receptors and the beta2 adrenergic receptor. Cell 117(6):833-46. [PubMed: 15186782] [MGI Ref ID J:91160]
Additional References
Olfr151tm13(Olr226)Mom relatedMarks CA; Cheng K; Cummings DM; Belluscio L. 2006. Activity-dependent plasticity in the olfactory intrabulbar map. J Neurosci 26(44):11257-66. [PubMed: 17079653] [MGI Ref ID J:114710]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, RG10/RG30.
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1980.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2574.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
General Supply Notes
- This strain is included in the Induced Mutant Resource Colony collection.
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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