Strain Name: |
B6;129P2-Omptm3Mom/MomJ |
|---|---|
Stock Number: |
006667 |
Availability: | Repository- Live |
Price and Supply Information | |
General Terms and Conditions |
| Strain Common Names | OMP-GFP; |
| Genes & Alleles | GFP; Omp; Omptm3Mom; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Targeted Mutation Mating System Heterozygote x Heterozygote (Female x Male) Species laboratory mouse Donating Investigator Peter Mombaerts, The Rockefeller University Generation N?+F1 (19-DEC-07) Strain Description
The coding region and part of the 3' non-translated region of the Omp gene was replaced by GFP. The targeted mutation results in a knockout. Mature olfactory sensory neurons express GFP at high levels. These neurons can be revealed by the intrinsic fluorescence of GFP, or by immunofluorescence with anti-GFP antibodies. Homozygous mice are subfertile.Strain Development
This mutation was generated in 129P2/OlaHsd derived E14 ES cells, the loxP flanked tk-neo cassette was excised by transient transfection of a plasmid expressing Cre recombinase, and the resulting mice were bred to C57BL/6 before intercrossing to homozygosity.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Omptm3Mom | ||
|---|---|---|---|
| Allele Name | targeted mutation 3, Peter Mombaerts | ||
| Common Name(s) | OMP-GFP; | ||
| Mutation Made By | Peter Mombaerts, The Rockefeller University | ||
| Strain of Origin | 129P2/OlaHsd | ||
| ES Cell Line Name | E14 | ||
| ES Cell Line Strain | 129P2/OlaHsd | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. The original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Gene Symbol and Name | Omp, olfactory marker protein | ||
| Chromosome | 7 | ||
| Molecular Note | The coding sequence was replaced with a GFP-LTNL sequence and the LTNL cassette was recombined out through the transfection of the targeted ES cells with cre recombinase. [MGI Ref ID J:73384] | ||
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for JAX® GEMM® Strains | ||
Genotyping Protocols
Omptm3Mom
Colony Maintenance
| Diet Information | LabDiet® 5K52/5K67 |
|---|
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (139 strains)
007768 B6.129S2-Omptm1Mom/MomJ 004946 B6;129P2-Omptm2(spH)Mom/J 006668 B6;129P2-Omptm4(cre)Mom/MomJ 006594 B6;129S2-Omptm1Mom/MomJ
Additional Web Information
Fluorescent Proteins/lacZ Systems
Animal Health Reports
Room Number AX11
Research Applications
This mouse can be used to support research in many areas including:
GFP relatedResearch Tools
Fluorescent Proteins
Omptm3Mom relatedResearch Tools
Fluorescent Proteins
Research Tools
Sensorineural Research
Sensorineural Research
References
Selected Reference(s)
Additional ReferencesPotter SM; Zheng C; Koos DS; Feinstein P; Fraser SE; Mombaerts P. 2001. Structure and emergence of specific olfactory glomeruli in the mouse. J Neurosci 21(24):9713-23. [PubMed: 11739580] [MGI Ref ID J:73384]
Price and Supply Information
| Strain Name: | B6;129P2-Omptm3Mom/MomJ |
| Stock Number: | 006667 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. The shipping destinations are:
- USA, Canada and Mexico
- International
*Pricing for Shipping Destination selected:
USA, Canada and Mexico
| Price(s) in US dollars ($) | Genotype(s) Provided | ||||
|---|---|---|---|---|---|
| Individual Mouse Price | $236.40 | Heterozygous for Omptm3Mom | |||
| Individual Mouse Price | $291.90 | Homozygous for Omptm3Mom | |||
| Pair | $472.80 | Heterozygous for Omptm3Mom x Heterozygous for Omptm3Mom | |||
Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
Histology and Tissue Collection Services are available for all JAX® Mice strains. For more information, please contact Customer Service at orderquest@jax.org or 1-800-422-6423. Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. View Control Pricing Information for JAX® Strains. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
Purchasing Information
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Contact Information
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Tel: 800.422.6423 or 207.288.5845
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