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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Donating Investigator Peter Mombaerts, Max Planck Institute of Biophysics Description
The coding region and part of the 3' non-translated region of the Omp gene was replaced by Cre. The targeted mutation results in a knockout. Mature olfactory sensory neurons express the Cre recombinase at high levels. Homozygous mice are subfertile.As an example, when crossed to a strain with widespread expression of GFP and a loxP-flanked Bgeo reporter (see Stock No. 004178), this mutant mouse strain may be useful in lineage tracing.
Development
The 129P2/OlaHsd derived E14 ES cell was used to generate this targeted mutation and the resulting mice were crossed to C57BL/6, and then intercrossed to homozygosity. The FRT-flanked neo cassette (FNF) is retained within this strain, and can be removed by flp-mediated recombination.The plasmid that was used to generate this mutation is banked and described at Addgene.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Omp
007768 B6.129S2-Omptm1Mom/MomJ 004946 B6;129P2-Omptm2(spH)Mom/J 006667 B6;129P2-Omptm3Mom/MomJ 006594 B6;129S2-Omptm1Mom/MomJ View Strains carrying other alleles of Omp (4 strains)
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
assigned by genotype
The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.Omptm4(cre)Mom/Omp+ Tg(CAG-Bgeo/GFP)21Lbe/0
involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ * C57BL/6 (conditional)
- no phenotypic analysis
- *normal* no phenotypic analysis (MGI Ref ID J:115857)
- mice were used to generate permanently marked nuclear transfer ES cells and chimaeric or clonal mice
This mouse can be used to support research in many areas including:cre related
Omptm4(cre)Mom relatedResearch Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Research Tools
Cre-lox System
Cre Recombinase Expression
Sensorineural Research
Sensorineural Research
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Omptm4(cre)Mom | ||
|---|---|---|---|
| Allele Name | targeted mutation 4, Peter Mombaerts | ||
| Allele Type | Targeted (knock-in) | ||
| Common Name(s) | OMP-Cre; | ||
| Mutation Made By | Peter Mombaerts, Max Planck Institute of Biophysics | ||
| Strain of Origin | 129P2/OlaHsd | ||
| ES Cell Line Name | E14 | ||
| ES Cell Line Strain | 129P2/OlaHsd | ||
| Site of Expression | mature olfactory sensory neurons | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Gene Symbol and Name | Omp, olfactory marker protein | ||
| Chromosome | 7 | ||
| Driver Note | Omp | ||
| Molecular Note | Sequence encoding cre recombinase and a neomycin resistance cassette flanked by FRT sites was substituted in place of the Omp coding region. [MGI Ref ID J:115857] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Omptm4(cre)Mom, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Li J; Ishii T; Feinstein P; Mombaerts P. 2004. Odorant receptor gene choice is reset by nuclear transfer from mouse olfactory sensory neurons. Nature 428(6981):393-9. [PubMed: 15042081] [MGI Ref ID J:115857]
Additional References
Omptm4(cre)Mom relatedCheng LE; Reed RR. 2007. Zfp423/OAZ participates in a developmental switch during olfactory neurogenesis. Neuron 54(4):547-57. [PubMed: 17521568] [MGI Ref ID J:122275]
Nam HS; Benezra R. 2009. High levels of Id1 expression define B1 type adult neural stem cells. Cell Stem Cell 5(5):515-26. [PubMed: 19896442] [MGI Ref ID J:154023]
Health & husbandry
Health & Colony Maintenance Information
Currently there no information available for this strain. This may be due to the supply level of this strain.
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Animals Provided
Price (US dollars $) Cryorecovery Fee $1900.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
| Pricing for International shipping destinations |
|
Animals Provided
Price (US dollars $) Cryorecovery Fee $2470.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
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Supply Details
| Standard Supply | Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
Purchasing Information
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Contact Information
Orders & Technical Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
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Terms of Use
Terms of Use
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For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
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Contact information
General inquiries
Contracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
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