Strain Name: |
B6;129P2-Omptm4(cre)Mom/MomJ |
|---|---|
Stock Number: |
006668 |
Availability: | Repository- Live |
Price and Supply Information | |
General Terms and Conditions |
| Strain Common Names | OMP-Cre; |
| Genes & Alleles | Omp; Omptm4(cre)Mom; cre; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Targeted Mutation Mating System Heterozygote x Heterozygote (Female x Male) Species laboratory mouse Donating Investigator Peter Mombaerts, The Rockefeller University Generation N?+F0 (08-JAN-08) Strain Description
The coding region and part of the 3' non-translated region of the Omp gene was replaced by Cre. The targeted mutation results in a knockout. Mature olfactory sensory neurons express the Cre recombinase at high levels. Homozygous mice are subfertile.As an example, when crossed to a strain with widespread expression of GFP and a loxP-flanked Bgeo reporter (see Stock No. 004178), this mutant mouse strain may be useful in lineage tracing.
Strain Development
The 129P2/OlaHsd derived E14 ES cell was used to generate this targeted mutation and the resulting mice were crossed to C57BL/6, and then intercrossed to homozygosity. The FRT-flanked neo cassette (FNF) is retained within this strain, and can be removed by flp-mediated recombination.The plasmid that was used to generate this mutation is banked and described at Addgene.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Omptm4(cre)Mom | ||
|---|---|---|---|
| Allele Name | targeted mutation 4, Peter Mombaerts | ||
| Common Name(s) | OMP-Cre; | ||
| Mutation Made By | Peter Mombaerts, The Rockefeller University | ||
| Strain of Origin | 129P2/OlaHsd | ||
| ES Cell Line Name | E14 | ||
| ES Cell Line Strain | 129P2/OlaHsd | ||
| Site of Expression | mature olfactory sensory neurons | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Gene Symbol and Name | Omp, olfactory marker protein | ||
| Chromosome | 7 | ||
| Molecular Note | Sequence encoding cre recombinase and a neomycin resistance cassette flanked by FRT sites was was substituted in place of the Omp coding region. [MGI Ref ID J:115857] | ||
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for JAX® GEMM® Strains | ||
Genotyping Protocols
Omptm4(cre)Mom
Colony Maintenance
| Diet Information | LabDiet® 5K52/5K67 |
|---|
Related Strains
Strains carrying other alleles of Omp
007768 B6.129S2-Omptm1Mom/MomJ 004946 B6;129P2-Omptm2(spH)Mom/J 006667 B6;129P2-Omptm3Mom/MomJ 006594 B6;129S2-Omptm1Mom/MomJ View Strains carrying other alleles of Omp (4 strains)
Additional Web Information
Cre-lox or FLP-FRT Systems
Animal Health Reports
Room Number AX12
Research Applications
This mouse can be used to support research in many areas including:cre related
Omptm4(cre)Mom relatedResearch Tools
Cre-lox System
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
Research Tools
Cre-lox System (Cre Recombinase Expression)
Sensorineural Research
Sensorineural Research
References
Selected Reference(s)
Additional ReferencesLi J; Ishii T; Feinstein P; Mombaerts P. 2004. Odorant receptor gene choice is reset by nuclear transfer from mouse olfactory sensory neurons. Nature 428(6981):393-9. [PubMed: 15042081] [MGI Ref ID J:115857]
Price and Supply Information
| Strain Name: | B6;129P2-Omptm4(cre)Mom/MomJ |
| Stock Number: | 006668 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. The shipping destinations are:
- USA, Canada and Mexico
- International
*Pricing for Shipping Destination selected:
USA, Canada and Mexico
| Price(s) in US dollars ($) | Genotype(s) Provided | ||||
|---|---|---|---|---|---|
| Individual Mouse Price | $236.40 | Heterozygous for Omptm4(cre)Mom | |||
| Individual Mouse Price | $291.90 | Homozygous for Omptm4(cre)Mom | |||
| Pair | $472.80 | Heterozygous for Omptm4(cre)Mom x Heterozygous for Omptm4(cre)Mom | |||
Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Histology and Tissue Collection Services are available for all JAX® Mice strains. For more information, please contact Customer Service at orderquest@jax.org or 1-800-422-6423. Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. View Control Pricing Information for JAX® Strains. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
- Use of MICE by companies or for-profit entities requires a license.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
Purchasing Information
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Surgical Services
Contact Information
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Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
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