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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Former Names STOCK-Olfr17tm7Mom/MomJ (Changed: 30-APR-07 ) Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse Donating Investigator Peter Mombaerts, Max Planck Institute of Biophysics Description
Olfactory sensory neurons that express the olfactory receptor Olfr17 also co-express the tauGFP fusion protein by virtue of IRES-mediated co-translation. These neurons can be revealed by the intrinsic fluorescence of GFP, or by immunofluorescence with anti-GFP antibodies.Development
Homologous recombination to generate this allele was done in 129P2/OlaHsd derived E14 ES cells. To excise the loxP-flanked neomycin resistance sequence, the resulting mouse was bred with a mouse carrying an EIIa-cre transgene (Lakso et al. 1996, Proceedings of the National Academy of Sciences, v93 p5860), a transgene generated in FVB/N but having been backcrossed at least four generations to C57BL/6 before this cross and having unknown pedigree prior to that. The resulting mice, lacking the neomycin resistance cassette, were intercrossed and homozygotes were selected that were devoid of the EIIa-cre transgene.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (169 strains)
007767 B6.129P2-Olfr17tm1Mom/MomJ 006595 B6;129P2-Olfr17tm1Mom/MomJ 006649 B6;129P2-Olfr17tm5Mom/MomJ 006650 STOCK Olfr17tm6Mom/MomJ 006651 STOCK-Olfr17tm4Mom/MomJ
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
Olfr17tm7Mom relatedResearch Tools
Fluorescent Proteins
Research Tools
Sensorineural Research
Sensorineural Research
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Olfr17tm7Mom | ||
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| Allele Name | targeted mutation 7, Peter Mombaerts | ||
| Allele Type | Targeted (Reporter) | ||
| Common Name(s) | P2-GFP; P2-IRES-tauGFP; P2-iTG; | ||
| Mutation Made By | Peter Mombaerts, Max Planck Institute of Biophysics | ||
| Strain of Origin | 129P2/OlaHsd | ||
| ES Cell Line Name | E14 | ||
| ES Cell Line Strain | 129P2/OlaHsd | ||
| Site of Expression | The tauGFP fusion protein is expressed in olfactory sensory neurons that express the olfactory receptor Olfr17. | ||
| Gene Symbol and Name | Olfr17, olfactory receptor 17 | ||
| Chromosome | 7 | ||
| Gene Common Name(s) | GA_x6K02T2PBJ9-9470146-9471093; JCG5; MOR263-5; OR10A4P; P2; ratchr1-164484422-164485369_ORF; | ||
| Molecular Note | An IRES-tauGFP was inserted 3 base pairs after the stop codon of the endogenous coding sequence yielding an insertional mutation that results in bicistronic expression of the endogenous gene and tauGFP [MGI Ref ID J:90716] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Olfr17tm7Mom, STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Feinstein P; Mombaerts P. 2004. A contextual model for axonal sorting into glomeruli in the mouse olfactory system. Cell 117(6):817-31. [PubMed: 15186781] [MGI Ref ID J:90716]
Health & husbandry
Health & Colony Maintenance Information
Currently there no information available for this strain. This may be due to the supply level of this strain.
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $1900.00
Additional Supply Details
| Pricing for International shipping destinations |
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*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $2470.00
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
See Terms of Use
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Terms of Use
Terms of Use
General Terms and Conditions
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Contact information
General inquiries
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| fax: | 207-288-6655 |
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