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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
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Description
Strain Information
Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Homozygote x Homozygote (Female x Male) 14-JUN-08 Species laboratory mouse Generation ?+N2F4 (18-NOV-09) Donating Investigator Peter Mombaerts, Max Planck Institute of Biophysics Description
Olfactory sensory neurons that express the olfactory receptor Olfr151 also co-express the tauGFP fusion protein by virtue of IRES-mediated co-translation. These neurons can be revealed by the intrinsic fluorescence of GFP, or by immunofluorescence with anti-GFP antibodies.Development
Homologous recombination to generate this allele was done in 129P2/OlaHsd derived E14 ES cells. The loxP-flanked neo cassette was then removed by transient transfection of cre recombinase prior to injection of these ES cells into blastocysts. The resulting mice were bred to C57BL/6 and intercrossed.The plasmid that was used to generate this mutation is banked and described at Addgene.
Control Information
| Control | ||
|---|---|---|
| None Available | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (225 strains)
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
assigned by genotype
Olfr151tm26Mom/Olfr151+
involves: 129P2/OlaHsd * C57BL/6
- nervous system phenotype
- abnormal olfactory bulb morphology (MGI Ref ID J:90716)
- glomeruli in heterozygotes containing the C57BL/6 pseudogene are reduced from ~50 um diameter to ~32 um in diameter
- abnormal sensory neuron innervation (MGI Ref ID J:90716)
- there is ~50% reduction of axonal inputs innervating glomeruli; all axons are of Olfr151
Olfr151tm26Mom/Olfr151tm26Mom
involves: 129P2/OlaHsd * C57BL/6
- no phenotypic analysis
- *normal* no phenotypic analysis (MGI Ref ID J:91160)
- used for expression analysis only
This mouse can be used to support research in many areas including:
Olfr151tm26Mom relatedResearch Tools
Fluorescent Proteins
Research Tools
Sensorineural Research
Sensorineural Research
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Olfr151tm26Mom | ||
|---|---|---|---|
| Allele Name | targeted mutation 26, Peter Mombaerts | ||
| Allele Type | Targeted (Reporter) | ||
| Common Name(s) | M71-G; M71-GFP; M71-IRES-tauGFP; Olfr7tm2Mom; | ||
| Strain of Origin | 129P2/OlaHsd | ||
| ES Cell Line Name | E14 | ||
| ES Cell Line Strain | 129P2/OlaHsd | ||
| Site of Expression | The tauGFP fusion protein is expressed in olfactory sensory neurons that express the olfactory receptor Olfr151. | ||
| Gene Symbol and Name | Olfr151, olfactory receptor 151 | ||
| Chromosome | 9 | ||
| Gene Common Name(s) | GA_x6K02T2PVTD-31408136-31407207; M71; MOR171-2; | ||
| Molecular Note | An IRES-tauGFP gene was inserted 3 nucleotides downstream of the endogenous stop codon. Crossing with EIIa-Cre mice excised a floxed neo at the 3' end of the inserted cassette. [MGI Ref ID J:76148] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Olfr151tm26Mom, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Feinstein P; Mombaerts P. 2004. A contextual model for axonal sorting into glomeruli in the mouse olfactory system. Cell 117(6):817-31. [PubMed: 15186781] [MGI Ref ID J:90716]
Additional References
Olfr151tm26Mom relatedBozza T; Feinstein P; Zheng C; Mombaerts P. 2002. Odorant receptor expression defines functional units in the mouse olfactory system. J Neurosci 22(8):3033-43. [PubMed: 11943806] [MGI Ref ID J:76148]
Cheng LE; Reed RR. 2007. Zfp423/OAZ participates in a developmental switch during olfactory neurogenesis. Neuron 54(4):547-57. [PubMed: 17521568] [MGI Ref ID J:122275]
Feinstein P; Bozza T; Rodriguez I; Vassalli A; Mombaerts P. 2004. Axon guidance of mouse olfactory sensory neurons by odorant receptors and the beta2 adrenergic receptor. Cell 117(6):833-46. [PubMed: 15186782] [MGI Ref ID J:91160]
Potter SM; Zheng C; Koos DS; Feinstein P; Fraser SE; Mombaerts P. 2001. Structure and emergence of specific olfactory glomeruli in the mouse. J Neurosci 21(24):9713-23. [PubMed: 11739580] [MGI Ref ID J:73384]
Rothman A; Feinstein P; Hirota J; Mombaerts P. 2005. The promoter of the mouse odorant receptor gene M71. Mol Cell Neurosci 28(3):535-46. [PubMed: 15737743] [MGI Ref ID J:115911]
Vassalli A; Rothman A; Feinstein P; Zapotocky M; Mombaerts P. 2002. Minigenes impart odorant receptor-specific axon guidance in the olfactory bulb. Neuron 35(4):681-96. [PubMed: 12194868] [MGI Ref ID J:78878]
Yan Z; Tan J; Qin C; Lu Y; Ding C; Luo M. 2008. Precise circuitry links bilaterally symmetric olfactory maps. Neuron 58(4):613-24. [PubMed: 18498741] [MGI Ref ID J:145291]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Mating System Homozygote x Homozygote (Female x Male) 14-JUN-08 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $160.40 Female or Male Homozygous for Olfr151tm26Mom
Pairs /Price (US dollars $) Pair Genotype $320.80 Homozygous for Olfr151tm26Mom x Homozygous for Olfr151tm26Mom
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $208.60 Female or Male Homozygous for Olfr151tm26Mom
Pairs /Price (US dollars $) Pair Genotype $417.10 Homozygous for Olfr151tm26Mom x Homozygous for Olfr151tm26Mom
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| None Available | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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