Strain Name: |
STOCK Olfr151tm28Mom/MomJ |
|---|---|
Stock Number: |
006677 |
Availability: | Repository- Live |
General Terms and Conditions |
| Strain Common Names | M71-IRES-Cre; |
| Genes & Alleles | Olfr151; Olfr151tm28Mom; cre; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Targeted Mutation Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Donating Investigator Peter Mombaerts, Max Planck Institute of Biophysics Generation ?+F1 (15-MAY-07) Strain Description
Olfactory sensory neurons that express the olfactory receptor Olfr151 also co-express the Cre recombinase by virtue of IRES-mediated co-translation.Strain Development
Homologous recombination to generate this allele was done in 129P2/OlaHsd derived E14 ES cells. The FRT-flanked neo cassette was removed from the resulting mutant mice by crossing the line to mice carrying Tg(ACTB-FLP). This transgene was then bred out of the line and through intercrossing it was made homozygous for Olfr151tm28Mom.
Gene & Allele Details
| Allele Symbol | Olfr151tm28Mom | ||
|---|---|---|---|
| Allele Name | targeted mutation 28, Peter Mombaerts | ||
| Common Name(s) | M71-IRES-Cre; | ||
| Strain of Origin | 129P2/OlaHsd | ||
| ES Cell Line Name | E14 | ||
| ES Cell Line Strain | 129P2/OlaHsd | ||
| Site of Expression | olfactory sensory neurons that express the olfactory receptor Olfr151 | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Gene Symbol and Name | Olfr151, olfactory receptor 151 | ||
| Chromosome | 9 | ||
| Gene Common Name(s) | GA_x6K02T2PVTD-31408136-31407207; M71; MOR171-2; OR11-318; OST025; | ||
| Molecular Note | An IRES-cre-FNF cassette was inserted downstream of the coding sequence of Olfr151 to permit bicistronic expression of Olfr151 and cre recombinase. The neo cassette was excised by crossing to mice bearing Tg(ACTB-FLP). [MGI Ref ID J:115857] | ||
Control Information
| Control | ||
|---|---|---|
| None Available | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Olfr151tm28Mom
Colony Maintenance
| Diet Information | LabDiet® 5K52/5K67 |
|---|
Related Strains
Strains carrying other alleles of Olfr151
View Strains carrying other alleles of Olfr151 (28 strains)
Additional Web Information
Cre-lox or FLP-FRT Systems
Animal Health Reports
Room Number AX11
Research Applications
This mouse can be used to support research in many areas including:cre related
Olfr151tm28Mom relatedResearch Tools
Cre-lox System
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
Research Tools
Cre-lox System (Cre Recombinase Expression)
Sensorineural Research
Sensorineural Research
References
Selected Reference(s)
Li J; Ishii T; Feinstein P; Mombaerts P. 2004. Odorant receptor gene choice is reset by nuclear transfer from mouse olfactory sensory neurons. Nature 428(6981):393-9. [PubMed: 15042081] [MGI Ref ID J:115857]
Price and Supply Information
| Strain Name: | STOCK Olfr151tm28Mom/MomJ |
| Stock Number: | 006677 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
- Use of MICE by companies or for-profit entities requires a license.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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