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- Description
- Disease & phenotype
- Genes & alleles
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Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Background Strain NOD/ShiLtJ Donor Strain 129 H2 Haplotype g7 Generation N15+N1F1pN1
Generation DefinitionsDonating Investigator Dr. Jeffrey A. Bluestone, University of California, San Francisco Appearance
albino, pink eyed
Related Genotype: A/A Tyrc/TyrcDescription
A "knock-in" replaces the endogenous gene with an Il4/IRES/EGFP bicistronic construct, which places both IL4 and EGFP expression under the control of the endogenous Il4 gene regulatory elements. IL4 activity in these mutant mice remains intact. Cells activated to express IL4 also express EGFP allowing reliable in vivo tracking of both innate and adaptive immune cells and enabling their isolation without further stimulation. Because the IRES element promotes translational competence capable of revealing low-level transcription otherwise not apparent from the canonical 5'-cap of the mRNA, cells from these mice also can be used to report competence for IL4 production upon stimulation. This knock-in allele has been backcrossed to NOD for 15 generations. Diabetes incidence in mutant mice is reported to be about 60%, which is not statistically different from wild-type cohorts or NOD inbred mice.Development
IL4, interleukin4, located on Chr.11, affects the growth and differentiation of B, T, and mast cells, and, IL4 receptors are expressed on B cells, T cells, mast cells, and macrophages. A targeting vector containing herpes simplex virus thymidine kinase, Il4 exon 3-4 sequence, a loxP-flanked neomycin cassette, and an IRES-EGFP construct was utilized in the making of this mutant. The vector was electroporated into 129T2 X 129S4-derived PrmCre embryonic stem (ES) cells. These ES cells express Cre recombinase under the direction of the mouse protamine 1 promoter (expresses in testis/male germline). Correctly targeted ES cells were injected into C57BL/6 blastocysts. The Il4tm1Lky mutation was transferred from a C57BL/6 background to NOD through 15 backcross generations. In 2007, the T1DR received hemizygous mice at generation N15 and mated to NOD/ShiLtJ prior to mating wild-type x hemizygous.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 001976 NOD/ShiLtJ | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (358 strains)
004190 C.129-Il4tm1Lky/J
002253 B6.129P2-Il4tm1Cgn/J 002496 BALB/c-Il4tm2Nnt/J 015859 C.129P2(Cg)-Il4/Il13tm1.1Lky/J 003480 C.129S2(B6)-Il4tm1Gru/J 002518 C57BL/6-Il4tm1Nnt/J 002230 C57BL/6J-Tg(LckIl4)1315Dbl/J 005879 D1Lac.Cg-Il4tm1Cgn/J 002574 NOD.129P2(B6)-Il4tm1Cgn/Dvs 004222 NOD.129P2(B6)-Il4tm1Cgn/DvsJ 004291 NOD.Cg-Il10tm1Cgn Il4tm1Cgn/DvsJ
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
GFP relatedDiabetes and Obesity Research
Type 1 Diabetes (IDDM) Analysis Strains
Immunology, Inflammation and Autoimmunity Research
Autoimmunity
Type 1 Diabetes
Intracellular Signaling Molecules
Research Tools
Fluorescent Proteins
Immunology and Inflammation Research
Il4tm1Lky relatedResearch Tools
Fluorescent Proteins
Cancer Research
Growth Factors/Receptors/Cytokines
Immunology, Inflammation and Autoimmunity Research
Growth Factors/Receptors/Cytokines
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Il4tm1Lky | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Richard M Locksley | ||
| Allele Type | Targeted (Reporter) | ||
| Common Name(s) | 4 get; 4get; | ||
| Mutation Made By | Markus Mohrs, Trudeau Institute, Inc. | ||
| Site of Expression | Cells activated to express IL-4 will express GFP. IL-4 activity remains intact. | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Molecular Note | A loxP-flanked neomycin cassette followed by an internal ribosomal entry site (IRES) and an EGFP gene was inserted into sequences corresponding to the 3' untranslated region in exon 4. The neomycin cassette was removed by Cre mediated germline recombination in chimeric mice to generate the final allele. These mice express green fluorescent protein under the control of the Il4 promoter. [MGI Ref ID J:75415] | ||
| Gene Symbol and Name | Il4, interleukin 4 | ||
| Chromosome | 11 | ||
| Gene Common Name(s) | BCGF-1; BCGF1; BSF-1; BSF1; IL-4; Il-4; Il4e12; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Il4tm1Lky, Separated PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Additional References
Il4tm1Lky relatedAu-Yeung BB; Fowell DJ. 2007. A key role for Itk in both IFN gamma and IL-4 production by NKT cells. J Immunol 179(1):111-9. [PubMed: 17579028] [MGI Ref ID J:143156]
Au-Yeung BB; Katzman SD; Fowell DJ. 2006. Cutting edge: Itk-dependent signals required for CD4+ T cells to exert, but not gain, Th2 effector function. J Immunol 176(7):3895-9. [PubMed: 16547221] [MGI Ref ID J:129883]
Cain JA; Smith JA; Ondr JK; Wang B; Katz JD. 2006. NKT cells and IFN-gamma establish the regulatory environment for the control of diabetogenic T cells in the nonobese diabetic mouse. J Immunol 176(3):1645-54. [PubMed: 16424194] [MGI Ref ID J:126603]
Cheng LE; Hartmann K; Roers A; Krummel MF; Locksley RM. 2013. Perivascular mast cells dynamically probe cutaneous blood vessels to capture immunoglobulin e. Immunity 38(1):166-75. [PubMed: 23290520] [MGI Ref ID J:193023]
Cheng LE; Wang ZE; Locksley RM. 2010. Murine B cells regulate serum IgE levels in a CD23-dependent manner. J Immunol 185(9):5040-7. [PubMed: 20870945] [MGI Ref ID J:165198]
Clay BS; Shilling RA; Bandukwala HS; Moore TV; Cannon JL; Welcher AA; Weinstock JV; Sperling AI. 2009. Inducible costimulator expression regulates the magnitude of Th2-mediated airway inflammation by regulating the number of Th2 cells. PLoS One 4(11):e7525. [PubMed: 19888475] [MGI Ref ID J:155428]
Dolgachev V; Petersen BC; Budelsky AL; Berlin AA; Lukacs NW. 2009. Pulmonary IL-17E (IL-25) production and IL-17RB+ myeloid cell-derived Th2 cytokine production are dependent upon stem cell factor-induced responses during chronic allergic pulmonary disease. J Immunol 183(9):5705-15. [PubMed: 19828636] [MGI Ref ID J:156804]
Everts B; Hussaarts L; Driessen NN; Meevissen MH; Schramm G; van der Ham AJ; van der Hoeven B; Scholzen T; Burgdorf S; Mohrs M; Pearce EJ; Hokke CH; Haas H; Smits HH; Yazdanbakhsh M. 2012. Schistosome-derived omega-1 drives Th2 polarization by suppressing protein synthesis following internalization by the mannose receptor. J Exp Med 209(10):1753-67, S1. [PubMed: 22966004] [MGI Ref ID J:191421]
Everts B; Perona-Wright G; Smits HH; Hokke CH; van der Ham AJ; Fitzsimmons CM; Doenhoff MJ; van der Bosch J; Mohrs K; Haas H; Mohrs M; Yazdanbakhsh M; Schramm G. 2009. Omega-1, a glycoprotein secreted by Schistosoma mansoni eggs, drives Th2 responses. J Exp Med 206(8):1673-80. [PubMed: 19635864] [MGI Ref ID J:151482]
Fairfax KC; Amiel E; King IL; Freitas TC; Mohrs M; Pearce EJ. 2012. IL-10R blockade during chronic schistosomiasis mansoni results in the loss of B cells from the liver and the development of severe pulmonary disease. PLoS Pathog 8(1):e1002490. [PubMed: 22291593] [MGI Ref ID J:195404]
Fallon PG; Ballantyne SJ; Mangan NE; Barlow JL; Dasvarma A; Hewett DR; McIlgorm A; Jolin HE; McKenzie AN. 2006. Identification of an interleukin (IL)-25-dependent cell population that provides IL-4, IL-5, and IL-13 at the onset of helminth expulsion. J Exp Med 203(4):1105-16. [PubMed: 16606668] [MGI Ref ID J:123748]
Fukuyama T; Kasper LH; Boussouar F; Jeevan T; van Deursen J; Brindle PK. 2009. Histone acetyltransferase CBP is vital to demarcate conventional and innate CD8+ T-cell development. Mol Cell Biol 29(14):3894-904. [PubMed: 19433445] [MGI Ref ID J:150146]
Girtsman T; Jaffar Z; Ferrini M; Shaw P; Roberts K. 2010. Natural Foxp3(+) regulatory T cells inhibit Th2 polarization but are biased toward suppression of Th17-driven lung inflammation. J Leukoc Biol 88(3):537-46. [PubMed: 20495073] [MGI Ref ID J:164926]
Hadeiba H; Locksley RM. 2003. Lung CD25 CD4 regulatory T cells suppress type 2 immune responses but not bronchial hyperreactivity. J Immunol 170(11):5502-10. [PubMed: 12759427] [MGI Ref ID J:109990]
Hammad H; Plantinga M; Deswarte K; Pouliot P; Willart MA; Kool M; Muskens F; Lambrecht BN. 2010. Inflammatory dendritic cells--not basophils--are necessary and sufficient for induction of Th2 immunity to inhaled house dust mite allergen. J Exp Med 207(10):2097-111. [PubMed: 20819925] [MGI Ref ID J:165817]
Hams E; McCarron MJ; Amu S; Yagita H; Azuma M; Chen L; Fallon PG. 2011. Blockade of B7-H1 (programmed death ligand 1) enhances humoral immunity by positively regulating the generation of T follicular helper cells. J Immunol 186(10):5648-55. [PubMed: 21490158] [MGI Ref ID J:173223]
Harris DP; Goodrich S; Mohrs K; Mohrs M; Lund FE. 2005. Cutting edge: the development of IL-4-producing B cells (B effector 2 cells) is controlled by IL-4, IL-4 receptor alpha, and Th2 cells. J Immunol 175(11):7103-7. [PubMed: 16301612] [MGI Ref ID J:122200]
Haynes NM; Allen CD; Lesley R; Ansel KM; Killeen N; Cyster JG. 2007. Role of CXCR5 and CCR7 in follicular Th cell positioning and appearance of a programmed cell death gene-1high germinal center-associated subpopulation. J Immunol 179(8):5099-108. [PubMed: 17911595] [MGI Ref ID J:153037]
Heib V; Becker M; Warger T; Rechtsteiner G; Tertilt C; Klein M; Bopp T; Taube C; Schild H; Schmitt E; Stassen M. 2007. Mast cells are crucial for early inflammation, migration of Langerhans cells, and CTL responses following topical application of TLR7 ligand in mice. Blood 110(3):946-53. [PubMed: 17446350] [MGI Ref ID J:145396]
Huber S; Hoffmann R; Muskens F; Voehringer D. 2010. Alternatively activated macrophages inhibit T-cell proliferation by Stat6-dependent expression of PD-L2. Blood 116(17):3311-20. [PubMed: 20625006] [MGI Ref ID J:165869]
Jang S; Schaller M; Berlin AA; Lukacs NW. 2010. Notch Ligand Delta-Like 4 Regulates Development and Pathogenesis of Allergic Airway Responses by Modulating IL-2 Production and Th2 Immunity. J Immunol 185(10):5835-44. [PubMed: 20944009] [MGI Ref ID J:165624]
Katzman SD; Fowell DJ. 2008. Pathogen-imposed skewing of mouse chemokine and cytokine expression at the infected tissue site. J Clin Invest 118(2):801-11. [PubMed: 18188454] [MGI Ref ID J:131191]
Kim-Saijo M; Janssen EM; Sugie K. 2008. CD4 cell-secreted, posttranslationally modified cytokine GIF suppresses Th2 responses by inhibiting the initiation of IL-4 production. Proc Natl Acad Sci U S A 105(49):19402-7. [PubMed: 19036925] [MGI Ref ID J:142099]
King IL; Mohrs M. 2009. IL-4-producing CD4+ T cells in reactive lymph nodes during helminth infection are T follicular helper cells. J Exp Med 206(5):1001-7. [PubMed: 19380638] [MGI Ref ID J:148500]
King SB; Knorn AM; Ohnmacht C; Voehringer D. 2008. Accumulation of effector CD4 T cells during type 2 immune responses is negatively regulated by Stat6. J Immunol 180(2):754-63. [PubMed: 18178813] [MGI Ref ID J:130967]
Liang HE; Reinhardt RL; Bando JK; Sullivan BM; Ho IC; Locksley RM. 2011. Divergent expression patterns of IL-4 and IL-13 define unique functions in allergic immunity. Nat Immunol 13(1):58-66. [PubMed: 22138715] [MGI Ref ID J:178986]
Liu AY; Dwyer DF; Jones TG; Bankova LG; Shen S; Katz HR; Austen KF; Gurish MF. 2013. Mast Cells Recruited to Mesenteric Lymph Nodes during Helminth Infection Remain Hypogranular and Produce IL-4 and IL-6. J Immunol 190(4):1758-66. [PubMed: 23319739] [MGI Ref ID J:193314]
Liu X; Yan X; Zhong B; Nurieva RI; Wang A; Wang X; Martin-Orozco N; Wang Y; Chang SH; Esplugues E; Flavell RA; Tian Q; Dong C. 2012. Bcl6 expression specifies the T follicular helper cell program in vivo. J Exp Med 209(10):1841-52, S1-24. [PubMed: 22987803] [MGI Ref ID J:191271]
Loke P; Gallagher I; Nair MG; Zang X; Brombacher F; Mohrs M; Allison JP; Allen JE. 2007. Alternative activation is an innate response to injury that requires CD4+ T cells to be sustained during chronic infection. J Immunol 179(6):3926-36. [PubMed: 17785830] [MGI Ref ID J:152038]
Loke P; Zang X; Hsuan L; Waitz R; Locksley RM; Allen JE; Allison JP. 2005. Inducible costimulator is required for type 2 antibody isotype switching but not T helper cell type 2 responses in chronic nematode infection. Proc Natl Acad Sci U S A 102(28):9872-7. [PubMed: 15994233] [MGI Ref ID J:99858]
Macleod MK; McKee AS; David A; Wang J; Mason R; Kappler JW; Marrack P. 2011. Vaccine adjuvants aluminum and monophosphoryl lipid A provide distinct signals to generate protective cytotoxic memory CD8 T cells. Proc Natl Acad Sci U S A 108(19):7914-9. [PubMed: 21518876] [MGI Ref ID J:172191]
McKee AS; MacLeod M; White J; Crawford F; Kappler JW; Marrack P. 2008. Gr1+IL-4-producing innate cells are induced in response to Th2 stimuli and suppress Th1-dependent antibody responses. Int Immunol 20(5):659-69. [PubMed: 18343889] [MGI Ref ID J:134492]
McKee AS; Munks MW; MacLeod MK; Fleenor CJ; Van Rooijen N; Kappler JW; Marrack P. 2009. Alum induces innate immune responses through macrophage and mast cell sensors, but these sensors are not required for alum to act as an adjuvant for specific immunity. J Immunol 183(7):4403-14. [PubMed: 19734227] [MGI Ref ID J:152787]
Mingueneau M; Roncagalli R; Gregoire C; Kissenpfennig A; Miazek A; Archambaud C; Wang Y; Perrin P; Bertosio E; Sansoni A; Richelme S; Locksley RM; Aguado E; Malissen M; Malissen B. 2009. Loss of the LAT adaptor converts antigen-responsive T cells into pathogenic effectors that function independently of the T cell receptor. Immunity 31(2):197-208. [PubMed: 19682930] [MGI Ref ID J:151840]
Mohrs K; Harris DP; Lund FE; Mohrs M. 2005. Systemic dissemination and persistence of Th2 and type 2 cells in response to infection with a strictly enteric nematode parasite. J Immunol 175(8):5306-13. [PubMed: 16210636] [MGI Ref ID J:119109]
Mohrs K; Wakil AE; Killeen N; Locksley RM; Mohrs M. 2005. A two-step process for cytokine production revealed by IL-4 dual-reporter mice. Immunity 23(4):419-29. [PubMed: 16226507] [MGI Ref ID J:113279]
Mohrs M; Shinkai K; Mohrs K; Locksley RM. 2001. Analysis of type 2 immunity in vivo with a bicistronic IL-4 reporter. Immunity 15(2):303-11. [PubMed: 11520464] [MGI Ref ID J:75415]
Moore ML; Newcomb DC; Parekh VV; Van Kaer L; Collins RD; Zhou W; Goleniewska K; Chi MH; Mitchell D; Boyce JA; Durbin JE; Sturkie C; Peebles RS Jr. 2009. STAT1 negatively regulates lung basophil IL-4 expression induced by respiratory syncytial virus infection. J Immunol 183(3):2016-26. [PubMed: 19587017] [MGI Ref ID J:151702]
Nel HJ; Hams E; Saunders SP; Mangan NE; Smith P; Atzberger A; Flavell RA; Akira S; McKenzie AN; Fallon PG. 2011. Impaired basophil induction leads to an age-dependent innate defect in type 2 immunity during helminth infection in mice. J Immunol 186(8):4631-9. [PubMed: 21398616] [MGI Ref ID J:172526]
Oboki K; Ohno T; Kajiwara N; Arae K; Morita H; Ishii A; Nambu A; Abe T; Kiyonari H; Matsumoto K; Sudo K; Okumura K; Saito H; Nakae S. 2010. IL-33 is a crucial amplifier of innate rather than acquired immunity. Proc Natl Acad Sci U S A 107(43):18581-6. [PubMed: 20937871] [MGI Ref ID J:165513]
Ohnmacht C; Schwartz C; Panzer M; Schiedewitz I; Naumann R; Voehringer D. 2010. Basophils orchestrate chronic allergic dermatitis and protective immunity against helminths. Immunity 33(3):364-74. [PubMed: 20817571] [MGI Ref ID J:164432]
Ohnmacht C; Voehringer D. 2009. Basophil effector function and homeostasis during helminth infection. Blood 113(12):2816-25. [PubMed: 18941115] [MGI Ref ID J:146344]
Parravicini V; Field AC; Tomlinson PD; Albert Basson M; Zamoyska R. 2008. Itch-/- alphabeta and gammadelta T cells independently contribute to autoimmunity in Itchy mice. Blood 111(8):4273-7282. [PubMed: 18256323] [MGI Ref ID J:134355]
Perona-Wright G; Mohrs K; Mayer KD; Mohrs M. 2010. Differential regulation of IL-4Ralpha expression by antigen versus cytokine stimulation characterizes Th2 progression in vivo. J Immunol 184(2):615-23. [PubMed: 20018622] [MGI Ref ID J:159399]
Perona-Wright G; Mohrs K; Mohrs M. 2010. Sustained signaling by canonical helper T cell cytokines throughout the reactive lymph node. Nat Immunol 11(6):520-6. [PubMed: 20418876] [MGI Ref ID J:160618]
Perona-Wright G; Mohrs K; Taylor J; Zaph C; Artis D; Pearce EJ; Mohrs M. 2008. Cutting edge: Helminth infection induces IgE in the absence of mu- or delta-chain expression. J Immunol 181(10):6697-701. [PubMed: 18981085] [MGI Ref ID J:140953]
Petersen BC; Budelsky AL; Baptist AP; Schaller MA; Lukacs NW. 2012. Interleukin-25 induces type 2 cytokine production in a steroid-resistant interleukin-17RB+ myeloid population that exacerbates asthmatic pathology. Nat Med 18(5):751-8. [PubMed: 22543263] [MGI Ref ID J:185138]
Phythian-Adams AT; Cook PC; Lundie RJ; Jones LH; Smith KA; Barr TA; Hochweller K; Anderton SM; Hammerling GJ; Maizels RM; MacDonald AS. 2010. CD11c depletion severely disrupts Th2 induction and development in vivo. J Exp Med 207(10):2089-96. [PubMed: 20819926] [MGI Ref ID J:194809]
Piehler D; Stenzel W; Grahnert A; Held J; Richter L; Kohler G; Richter T; Eschke M; Alber G; Muller U. 2011. Eosinophils Contribute to IL-4 Production and Shape the T-Helper Cytokine Profile and Inflammatory Response in Pulmonary Cryptococcosis. Am J Pathol 179(2):733-44. [PubMed: 21699881] [MGI Ref ID J:174409]
Price AE; Liang HE; Sullivan BM; Reinhardt RL; Eisley CJ; Erle DJ; Locksley RM. 2010. Systemically dispersed innate IL-13-expressing cells in type 2 immunity. Proc Natl Acad Sci U S A 107(25):11489-94. [PubMed: 20534524] [MGI Ref ID J:161387]
Rangel-Moreno J; Moyron-Quiroz JE; Carragher DM; Kusser K; Hartson L; Moquin A; Randall TD. 2009. Omental milky spots develop in the absence of lymphoid tissue-inducer cells and support B and T cell responses to peritoneal antigens. Immunity 30(5):731-43. [PubMed: 19427241] [MGI Ref ID J:149548]
Reese TA; Liang HE; Tager AM; Luster AD; Van Rooijen N; Voehringer D; Locksley RM. 2007. Chitin induces accumulation in tissue of innate immune cells associated with allergy. Nature 447(7140):92-6. [PubMed: 17450126] [MGI Ref ID J:122735]
Reinhardt RL; Hong S; Kang SJ; Wang ZE; Locksley RM. 2006. Visualization of IL-12/23p40 in vivo reveals immunostimulatory dendritic cell migrants that promote Th1 differentiation. J Immunol 177(3):1618-27. [PubMed: 16849470] [MGI Ref ID J:112745]
Reinhardt RL; Liang HE; Locksley RM. 2009. Cytokine-secreting follicular T cells shape the antibody repertoire. Nat Immunol 10(4):385-93. [PubMed: 19252490] [MGI Ref ID J:147784]
Scheu S; Stetson DB; Reinhardt RL; Leber JH; Mohrs M; Locksley RM. 2006. Activation of the integrated stress response during T helper cell differentiation. Nat Immunol 7(6):644-51. [PubMed: 16680145] [MGI Ref ID J:112676]
Seidl A; Panzer M; Voehringer D. 2011. Protective immunity against the gastrointestinal nematode Nippostrongylus brasiliensis requires a broad T-cell receptor repertoire. Immunology 134(2):214-23. [PubMed: 21896015] [MGI Ref ID J:179222]
Sofi MH; Qiao Y; Ansel KM; Kubo M; Chang CH. 2011. Induction and Maintenance of IL-4 Expression Are Regulated Differently by the 3' Enhancer in CD4 T Cells. J Immunol 186(5):2792-9. [PubMed: 21282512] [MGI Ref ID J:169377]
Sokol CL; Barton GM; Farr AG; Medzhitov R. 2008. A mechanism for the initiation of allergen-induced T helper type 2 responses. Nat Immunol 9(3):310-8. [PubMed: 18300366] [MGI Ref ID J:131552]
Sokol CL; Chu NQ; Yu S; Nish SA; Laufer TM; Medzhitov R. 2009. Basophils function as antigen-presenting cells for an allergen-induced T helper type 2 response. Nat Immunol 10(7):713-20. [PubMed: 19465907] [MGI Ref ID J:150141]
Sullivan BM; Liang HE; Bando JK; Wu D; Cheng LE; McKerrow JK; Allen CD; Locksley RM. 2011. Genetic analysis of basophil function in vivo. Nat Immunol 12(6):527-35. [PubMed: 21552267] [MGI Ref ID J:172122]
Tang H; Cao W; Kasturi SP; Ravindran R; Nakaya HI; Kundu K; Murthy N; Kepler TB; Malissen B; Pulendran B. 2010. The T helper type 2 response to cysteine proteases requires dendritic cell-basophil cooperation via ROS-mediated signaling. Nat Immunol 11(7):608-17. [PubMed: 20495560] [MGI Ref ID J:161857]
Van Dyken SJ; Garcia D; Porter P; Huang X; Quinlan PJ; Blanc PD; Corry DB; Locksley RM. 2011. Fungal chitin from asthma-associated home environments induces eosinophilic lung infiltration. J Immunol 187(5):2261-7. [PubMed: 21824866] [MGI Ref ID J:179262]
Veldhoen M; Uyttenhove C; van Snick J; Helmby H; Westendorf A; Buer J; Martin B; Wilhelm C; Stockinger B. 2008. Transforming growth factor-beta 'reprograms' the differentiation of T helper 2 cells and promotes an interleukin 9-producing subset. Nat Immunol 9(12):1341-6. [PubMed: 18931678] [MGI Ref ID J:143562]
Voehringer D; Reese TA; Huang X; Shinkai K; Locksley RM. 2006. Type 2 immunity is controlled by IL-4/IL-13 expression in hematopoietic non-eosinophil cells of the innate immune system. J Exp Med 203(6):1435-46. [PubMed: 16702603] [MGI Ref ID J:124383]
Voehringer D; Rosen DB; Lanier LL; Locksley RM. 2004. CD200 receptor family members represent novel DAP12-associated activating receptors on basophils and mast cells. J Biol Chem 279(52):54117-23. [PubMed: 15471863] [MGI Ref ID J:95161]
Voehringer D; Stanley SA; Cox JS; Completo GC; Lowary TL; Locksley RM. 2007. Nippostrongylus brasiliensis: identification of intelectin-1 and -2 as Stat6-dependent genes expressed in lung and intestine during infection. Exp Parasitol 116(4):458-66. [PubMed: 17420014] [MGI Ref ID J:128858]
Voehringer D; van Rooijen N; Locksley RM. 2007. Eosinophils develop in distinct stages and are recruited to peripheral sites by alternatively activated macrophages. J Leukoc Biol 81(6):1434-44. [PubMed: 17339609] [MGI Ref ID J:122340]
Wang Y; Souabni A; Flavell RA; Wan YY. 2010. An intrinsic mechanism predisposes foxp3-expressing regulatory T cells to th2 conversion in vivo. J Immunol 185(10):5983-92. [PubMed: 20944002] [MGI Ref ID J:165629]
Wiethe C; Debus A; Mohrs M; Steinkasserer A; Lutz M; Gessner A. 2008. Dendritic Cell Differentiation State and Their Interaction with NKT Cells Determine Th1/Th2 Differentiation in the Murine Model of Leishmania major Infection. J Immunol 180(7):4371-81. [PubMed: 18354157] [MGI Ref ID J:132974]
Wilson EH; Zaph C; Mohrs M; Welcher A; Siu J; Artis D; Hunter CA. 2006. B7RP-1-ICOS interactions are required for optimal infection-induced expansion of CD4+ Th1 and Th2 responses. J Immunol 177(4):2365-72. [PubMed: 16887998] [MGI Ref ID J:138389]
Wojciechowski W; Harris DP; Sprague F; Mousseau B; Makris M; Kusser K; Honjo T; Mohrs K; Mohrs M; Randall T; Lund FE. 2009. Cytokine-producing effector B cells regulate type 2 immunity to H. polygyrus. Immunity 30(3):421-33. [PubMed: 19249230] [MGI Ref ID J:146768]
Wu D; Molofsky AB; Liang HE; Ricardo-Gonzalez RR; Jouihan HA; Bando JK; Chawla A; Locksley RM. 2011. Eosinophils sustain adipose alternatively activated macrophages associated with glucose homeostasis. Science 332(6026):243-7. [PubMed: 21436399] [MGI Ref ID J:170307]
Yang XO; Angkasekwinai P; Zhu J; Peng J; Liu Z; Nurieva R; Liu X; Chung Y; Chang SH; Sun B; Dong C. 2009. Requirement for the basic helix-loop-helix transcription factor Dec2 in initial TH2 lineage commitment. Nat Immunol 10(12):1260-6. [PubMed: 19881507] [MGI Ref ID J:157740]
Zaretsky AG; Taylor JJ; King IL; Marshall FA; Mohrs M; Pearce EJ. 2009. T follicular helper cells differentiate from Th2 cells in response to helminth antigens. J Exp Med 206(5):991-9. [PubMed: 19380637] [MGI Ref ID J:148501]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry Both heterozygous and homozygous mice are reported to be viable and fertile.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2085.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2710.50 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
General Supply Notes
- This strain is included in the Type 1 Diabetes Repository collection.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 001976 NOD/ShiLtJ | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form
Terms of Use
Terms of Use
General Terms and Conditions
For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
Contact information
General inquiries regarding Terms of UseContracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.
No Liability
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.