Strain Name:

STOCK Nrp2tm1.2Mom/MomJ

Stock Number:

006700

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Availability:

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Common Names: Neuropilin-2-delta;    

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
 
Donating Investigator Peter Mombaerts,   Max Planck Research Unit for Neurogenetics

Development
The Nrp2 targeted mutation was generated in 129P2/OlaHsd derived E14 ES cells and has a loxP-tauGFP-pA+-FNF cassette inserted into the first intron and a corresponding loxP site inserted upstream of the start codon. The FRT-flanked neomycin resistance sequence (FNF) was excised by crossing to mice with the Tg(ACTB-flp)4917Dym transgene insertion, which was generated in a mixed B6;SJL background, yielding the Nrp2 targeted mutation on a mixed background that is predominantly 129P2 and C57BL/6. The transgene was selectively bred out of the line. Exon 1 and the first part of intron 1 were excised by cre recombination by breeding to mice carrying Tg(EIIa-cre)C5379Lmgd, a transgene generated in FVB/N but having been backcrossed at least four generations to C57BL/6 before this cross and having unknown pedigree prior to that. The resulting offspring, bearing the Nrp2tm1.2 mutation, were intercrossed and the transgene was selectively bred out of the line.

Related Strains

Strains carrying other alleles of Nrp2
006697   STOCK Nrp2tm1.1Mom/MomJ
View Strains carrying other alleles of Nrp2     (1 strain)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Model with phenotypic similarity to human disease where etiologies are distinct. Human genes are associated with this disease. Orthologs of these genes do not appear in the mouse genotype(s).
Autism
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Nrp2tm1.2Mom/Nrp2+

        involves: 129P2/OlaHsd * C57BL/6 * FVB/NJ
  • behavior/neurological phenotype
  • seizures   (MGI Ref ID J:166116)
    • environmentally induced seizures
      • 14 of 273 mice exhibit random handling-induced seizures   (MGI Ref ID J:166116)
    • increased susceptibility to pharmacologically induced seizures
      • 7 of 9 mutants treated with kainic acid (KA) to induce seizures, develop at least two spontaneous seizures that qualify as epilepsy   (MGI Ref ID J:166116)
      • mutants treated with KA to induce seizures exhibit a shorter latency to onset of seizures   (MGI Ref ID J:166116)
      • mutants treated with pentylenetretrazol (PTZ) to induce seizures exhibit a significantly shorter latency to onset of seizures than controls but exhibit a similar number of acute seizures as controls   (MGI Ref ID J:166116)
    • myoclonus
      • 14 of 273 mice exhibit random handling-induced seizures   (MGI Ref ID J:166116)
  • muscle phenotype
  • myoclonus
    • 14 of 273 mice exhibit random handling-induced seizures   (MGI Ref ID J:166116)
  • nervous system phenotype
  • abnormal dendrite morphology
    • moderate decrease in dendritic branching and total dendritic length of CA1 pyramidal cells   (MGI Ref ID J:166116)
    • abnormal dendritic spine morphology
      • N type and D type spines are increased 11% compared to CA1 neurons from wild-type   (MGI Ref ID J:166116)
  • abnormal nervous system physiology
    • mutants exhibit enhanced excitability in the hippocampus as indicated by an increase in population spike (PS) amplitude   (MGI Ref ID J:166116)
    • abnormal synaptic plasticity
      • short-term plasticity is altered as indicated by a decrease in the facilitation of the secondary population spike (PS) elicited during the 7 Hz stimulation train   (MGI Ref ID J:166116)
    • seizures   (MGI Ref ID J:166116)
      • environmentally induced seizures
        • 14 of 273 mice exhibit random handling-induced seizures   (MGI Ref ID J:166116)
      • increased susceptibility to pharmacologically induced seizures
        • 7 of 9 mutants treated with kainic acid (KA) to induce seizures, develop at least two spontaneous seizures that qualify as epilepsy   (MGI Ref ID J:166116)
        • mutants treated with KA to induce seizures exhibit a shorter latency to onset of seizures   (MGI Ref ID J:166116)
        • mutants treated with pentylenetretrazol (PTZ) to induce seizures exhibit a significantly shorter latency to onset of seizures than controls but exhibit a similar number of acute seizures as controls   (MGI Ref ID J:166116)
      • myoclonus
        • 14 of 273 mice exhibit random handling-induced seizures   (MGI Ref ID J:166116)

Nrp2tm1.2Mom/Nrp2tm1.2Mom

        involves: 129P2/OlaHsd * C57BL/6 * SJL/J
  • mortality/aging
  • partial prenatal lethality
    • homozygous mutant pups are born alive at a reduced frequency (10.9% vs expected 25%); however, no increased mortality is noted in juvenile or adult mice   (MGI Ref ID J:76686)
  • nervous system phenotype
  • abnormal axon guidance
    • homozygotes display aberrant axonal innervation of the main olfactory bulb and accessory olfactory bulb by olfactory sensory neurons and vomeronasal sensory neurons, respectively   (MGI Ref ID J:76686)
  • abnormal sensory neuron innervation pattern
    • in the vomeronasal system, some apical vomeronasal sensory neuron axons are misrouted and innervate glomeruli in an ectopic domain of the accessory olfactory bulb   (MGI Ref ID J:76686)
    • abnormal olfactory neuron innervation pattern
      • homozygotes display marked and distinct abnormalities on target innervation within the olfactory bulb   (MGI Ref ID J:76686)
      • in the main olfactory system, axons of mutant olfactory sensory neurons penetrate into the deeper layers of the main olfactory bulb, overshooting their glomerular target and extending into the external plexiform layer   (MGI Ref ID J:76686)
  • abnormal vomeronasal organ morphology
    • in the vomeronasal system, homozygotes exhibit axonal defasciculation within the vomeronasal nerve   (MGI Ref ID J:76686)
  • hydroencephaly
    • 63.6% of homozygotes exhibit hydrocephalus (not observed in heterozygotes)   (MGI Ref ID J:76686)
  • growth/size/body phenotype
  • abnormal vomeronasal organ morphology
    • in the vomeronasal system, homozygotes exhibit axonal defasciculation within the vomeronasal nerve   (MGI Ref ID J:76686)
  • postnatal growth retardation
    • at P2-P3, mutant pups display a striking reduction of overall body size, resulting in ~50% of normal size at 3 weeks of age   (MGI Ref ID J:76686)
    • however, mutant mice catch up with their wild-type littermates at weaning, and exhibit a normal body size after 6-8 weeks   (MGI Ref ID J:76686)
  • reproductive system phenotype
  • reduced fertility
    • homozygotes often are not able to reproduce   (MGI Ref ID J:76686)
  • respiratory system phenotype
  • abnormal vomeronasal organ morphology
    • in the vomeronasal system, homozygotes exhibit axonal defasciculation within the vomeronasal nerve   (MGI Ref ID J:76686)
  • craniofacial phenotype
  • abnormal vomeronasal organ morphology
    • in the vomeronasal system, homozygotes exhibit axonal defasciculation within the vomeronasal nerve   (MGI Ref ID J:76686)
  • cellular phenotype
  • abnormal axon guidance
    • homozygotes display aberrant axonal innervation of the main olfactory bulb and accessory olfactory bulb by olfactory sensory neurons and vomeronasal sensory neurons, respectively   (MGI Ref ID J:76686)

Nrp2tm1.2Mom/Nrp2tm1.2Mom

        involves: 129P2/OlaHsd * C57BL/6 * FVB/NJ
  • mortality/aging
  • increased sensitivity to induced morbidity/mortality
    • all mutants treated with KA to induce seizures die within a week   (MGI Ref ID J:166116)
  • partial postnatal lethality
    • some mice die in spontaneous status epilepticus between P7 and P21   (MGI Ref ID J:166116)
  • behavior/neurological phenotype
  • seizures   (MGI Ref ID J:166116)
    • environmentally induced seizures
      • 12 of 51 mice exhibit random handling-induced seizures   (MGI Ref ID J:166116)
    • increased susceptibility to pharmacologically induced seizures
      • all mutants treated with kainic acid (KA) to induce seizures die within a week   (MGI Ref ID J:166116)
    • myoclonus
      • 12 of 51 mice exhibit random handling-induced seizures   (MGI Ref ID J:166116)
  • muscle phenotype
  • myoclonus
    • 12 of 51 mice exhibit random handling-induced seizures   (MGI Ref ID J:166116)
  • nervous system phenotype
  • abnormal hippocampus neuron morphology
    • mutants exhibit selective loss of GABAergic cells in the hippocampus   (MGI Ref ID J:166116)
    • Parv+ and NPY+ neurons are reduced in the hippocampus   (MGI Ref ID J:166116)
  • seizures   (MGI Ref ID J:166116)
    • environmentally induced seizures
      • 12 of 51 mice exhibit random handling-induced seizures   (MGI Ref ID J:166116)
    • increased susceptibility to pharmacologically induced seizures
      • all mutants treated with kainic acid (KA) to induce seizures die within a week   (MGI Ref ID J:166116)
    • myoclonus
      • 12 of 51 mice exhibit random handling-induced seizures   (MGI Ref ID J:166116)

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Nrp2tm1.2Mom
Allele Name targeted mutation 1.2, Peter Mombaerts
Allele Type Targeted (Null/Knockout, Reporter)
Common Name(s) NP2-delta; Neuropilin-2-Delta; Nrp2del; Nrp2tm1Mom;
Mutation Made By Peter Mombaerts,   Max Planck Research Unit for Neurogenetics
Strain of Origin129P2/OlaHsd
ES Cell Line NameE14
ES Cell Line Strain129P2/OlaHsd
Gene Symbol and Name Nrp2, neuropilin 2
Chromosome 1
Gene Common Name(s) 1110048P06Rik; NP-2; NP2; NPN2; Npn-2; PRO2714; RIKEN cDNA 1110048P06 gene; VEGF165R2;
Molecular Note A loxP site 272 bp upstream of the translation initiation codon was introduced into exon 1 and a loxP-GFP FRT-flanked neo cassette was inserted 1 kb into intron 1 via homologous recombination. Heterozygous animals were crossed to an flp recombinase transgenic line for removal of the neo cassette. Subsequent mating to a cre recombinase transgenic line removed exon 1 and part of intron 1 leaving the GFP cassette intact. The exon 1 deletion removes the plasma membrane-targeting signal sequence and also brings the GFP cassette under the control of the endogenous promoter. RT-PCR analysis confirmed the removal of the signal sequence in gene transcripts from homozygous mutant animals. Immunohistochemistry of brain sections from homozygous mutants did not detect protein product. [MGI Ref ID J:76686]

Genotyping

Genotyping Information

Genotyping Protocols

Nrp2tm1.2Mom STD PCR, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Walz A; Rodriguez I; Mombaerts P. 2002. Aberrant sensory innervation of the olfactory bulb in neuropilin-2 mutant mice. J Neurosci 22(10):4025-35. [PubMed: 12019322]  [MGI Ref ID J:76686]

Additional References

Nrp2tm1.2Mom related

Bielenberg DR; Seth A; Shimizu A; Pelton K; Cristofaro V; Ramachandran A; Zwaans BM; Chen C; Krishnan R; Seth M; Huang L; Takashima S; Klagsbrun M; Sullivan MP; Adam RM. 2012. Increased smooth muscle contractility in mice deficient for neuropilin 2. Am J Pathol 181(2):548-59. [PubMed: 22688055]  [MGI Ref ID J:186996]

Gant JC; Thibault O; Blalock EM; Yang J; Bachstetter A; Kotick J; Schauwecker PE; Hauser KF; Smith GM; Mervis R; Li Y; Barnes GN. 2009. Decreased number of interneurons and increased seizures in neuropilin 2 deficient mice: implications for autism and epilepsy. Epilepsia 50(4):629-45. [PubMed: 18657176]  [MGI Ref ID J:166116]

Leinders-Zufall T; Ishii T; Chamero P; Hendrix P; Oboti L; Schmid A; Kircher S; Pyrski M; Akiyoshi S; Khan M; Vaes E; Zufall F; Mombaerts P. 2014. A family of nonclassical class I MHC genes contributes to ultrasensitive chemodetection by mouse vomeronasal sensory neurons. J Neurosci 34(15):5121-33. [PubMed: 24719092]  [MGI Ref ID J:207447]

Matsuo T; Rossier DA; Kan C; Rodriguez I. 2012. The wiring of Grueneberg ganglion axons is dependent on neuropilin 1. Development 139(15):2783-91. [PubMed: 22745317]  [MGI Ref ID J:185650]

Takahashi H; Yoshihara S; Nishizumi H; Tsuboi A. 2010. Neuropilin-2 is required for the proper targeting of ventral glomeruli in the mouse olfactory bulb. Mol Cell Neurosci 44(3):233-45. [PubMed: 20363325]  [MGI Ref ID J:164129]

Walz A; Feinstein P; Khan M; Mombaerts P. 2007. Axonal wiring of guanylate cyclase-D-expressing olfactory neurons is dependent on neuropilin 2 and semaphorin 3F. Development 134:4063-72. [PubMed: 17942483]  [MGI Ref ID J:125485]

Walz A; Mombaerts P; Greer CA; Treloar HB. 2006. Disrupted compartmental organization of axons and dendrites within olfactory glomeruli of mice deficient in the olfactory cell adhesion molecule, OCAM. Mol Cell Neurosci 32(1-2):1-14. [PubMed: 16531066]  [MGI Ref ID J:111928]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2140.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2782.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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