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Type Mutant Stock; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Donating Investigator Peter Mombaerts, Max Planck Institute of Biophysics Development
The Olfr160 coding sequence was precisely replaced with that of Olfr545 and an IRES-tauGFP and self-excising neomycin resistance cassette were inserted 3 nucleotides downstream of the stop codon. This construct was used for homologous recombination in 129P2/OlaHsd derived E14 ES cells and a founder from clone 5072-15 was bred to C57BL/6J females.
Strains carrying other alleles of GFP
View Strains carrying other alleles of GFP (314 strains)
Strains carrying other alleles of Olfr160
View Strains carrying other alleles of Olfr160 (13 strains)
Strains carrying other alleles of Olfr545
006712 B6;129P2-Olfr545tm1Mom/MomJ 006713 B6;129P2-Olfr545tm2Mom/MomJ 006715 B6;129P2-Olfr545tm3(Olfr160)Mom/MomJ 006716 B6;129P2-Olfr545tm4Mom/MomJ View Strains carrying other alleles of Olfr545 (4 strains)
View Research Applications
Currently there is no phenotype information for this strain.Research Applications
This mouse can be used to support research in many areas including:GFP related
Research Tools
Fluorescent Proteins
| Allele Symbol | Olfr160tm11(Olfr545)Mom | ||
|---|---|---|---|
| Allele Name | targeted mutation 11, Peter Mombaerts | ||
| Allele Type | Targeted (knock-in) | ||
| Common Name(s) | S50->M72-IRES-tauGFP; | ||
| Strain of Origin | 129P2/OlaHsd | ||
| ES Cell Line Name | E14 | ||
| ES Cell Line Strain | 129P2/OlaHsd | ||
| Site of Expression | GFP is expressed in olfactory sensory neurons. | ||
| Expressed Gene | Olfr545, olfactory receptor 545, mouse, laboratory | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Molecular Note | The endogenous coding sequence was entirely replaced with that of Olfr545, an IRES-tauGFP and self-excising neomycin resistance cassette were placed downstream, and clone 5072-15 was used to generate this allele. [MGI Ref ID J:144169] | ||
| Gene Symbol and Name | Olfr160, olfactory receptor 160 | ||
| Chromosome | 9 | ||
| Gene Common Name(s) | GA_x6K02T2PVTD-31389446-31388517; M72; MOR171-3; OR11-318; OST025; | ||
Genotyping Protocols
Olfr160tm11(Olfr545)Mom, Separated PCR
Helpful Links
Genotyping resources and troubleshooting
Bozza T; Vassalli A; Fuss S; Zhang JJ; Weiland B; Pacifico R; Feinstein P; Mombaerts P. 2009. Mapping of Class I and Class II Odorant Receptors to Glomerular Domains by Two Distinct Types of Olfactory Sensory Neurons in the Mouse. Neuron 61(2):220-33. [PubMed: 19186165] [MGI Ref ID J:144169]
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2250.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
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Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2925.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
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