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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Mutant Stock; Targeted Mutation; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Donating Investigator Peter Mombaerts, The Rockefeller University Development
A taulacZ cassette was inserted downstream of a minigene of 9.2 kb of genomic sequence that included the coding and regulatory sequence Olfr151 and the construct was microinjected into (CBA x C57BL/6J)F2 pronuclei. Several founders were obtained and the lines were bred to a strain bearing Olfr160tm1Mom (commonly referred to as M71->M72-IRES-tauGFP), which was generated in 129P2/OlaHsd-derived E14 ES cells and outcrossed to C57BL/6J. In 2007 sperm was cryopreserved from males carrying both the transgene and the targeted mutation.
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (225 strains)
006740 STOCK Olfr160tm1Mom Tg(Olfr151,taulacZ)AMom/MomJ 006647 STOCK Olfr160tm1Mom/MomJ
007766 B6.129P2(Cg)-Olfr160tm6Mom/MomJ 006714 B6;129P2-Olfr160tm11(Olfr545)Mom/MomJ 006596 B6;129P2-Olfr160tm4Mom/MomJ 006687 B6;129P2-Olfr160tm7Mom/MomJ 006652 STOCK Olfr160tm2(GFP)Mom/MomJ 006696 STOCK Olfr160tm3Mom/MomJ 006636 STOCK Olfr160tm5(Cnga2)Mom/MomJ 006678 STOCK Olfr160tm6Mom/MomJ
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
Currently there is no phenotype information for this strain.This mouse can be used to support research in many areas including:GFP related
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Olfr160tm1Mom | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Peter Mombaerts | ||
| Allele Type | Targeted (knock-in) | ||
| Common Name(s) | M71-M72-GFP; M71-M72-IRES-tauGFP; M71-M72; | ||
| Mutation Made By | Peter Mombaerts, The Rockefeller University | ||
| Strain of Origin | 129P2/OlaHsd | ||
| ES Cell Line Name | E14 | ||
| ES Cell Line Strain | 129P2/OlaHsd | ||
| Gene Symbol and Name | Olfr160, olfactory receptor 160 | ||
| Chromosome | 9 | ||
| Gene Common Name(s) | GA_x6K02T2PVTD-31389446-31388517; M72; MOR171-3; OR11-318; OST025; | ||
| Molecular Note | The entire coding region of Olfr160 was replaced with Olfr151 and an IRES-tauGFP gene introduced via homologous recombination. The lacZ gene was fused after the stop codon and co-expressed with the mutant protein. [MGI Ref ID J:90716] | ||
| Allele Symbol | Tg(Olfr151,taulacZ)BMom | ||
| Allele Name | transgene insertion B, Peter Mombaerts | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | M71-TgB; | ||
| Site of Expression | GFP is expressed in olfactory sensory neurons that express the olfactory receptor Olfr151. LacZ is expressed in Zone 4 of the olfactory epithelium. | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Promoter | Olfr151, olfactory receptor 151, mouse, laboratory | ||
| Molecular Note | This transgene, generated through pronuclear injection, has 9.2 kb of genomic sequence, with 2.3 kb upstream of the putative transcription start site and 3.8 kb downstream of the coding region, and a taulacZ cassette inserted downstream of the coding sequence. [MGI Ref ID J:78878] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Olfr160tm1Mom, Separated PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Vassalli A; Rothman A; Feinstein P; Zapotocky M; Mombaerts P. 2002. Minigenes impart odorant receptor-specific axon guidance in the olfactory bulb. Neuron 35(4):681-96. [PubMed: 12194868] [MGI Ref ID J:78878]
Additional References
Olfr160tm1Mom relatedFeinstein P; Mombaerts P. 2004. A contextual model for axonal sorting into glomeruli in the mouse olfactory system. Cell 117(6):817-31. [PubMed: 15186781] [MGI Ref ID J:90716]
Health & husbandry
Health & Colony Maintenance Information
Currently there no information available for this strain. This may be due to the supply level of this strain.
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Animals Provided
Price (US dollars $) Cryorecovery Fee $1900.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
| Pricing for International shipping destinations |
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Animals Provided
Price (US dollars $) Cryorecovery Fee $2470.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
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Supply Details
| Standard Supply | Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
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Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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