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| These "Foxp3EGFP" mice co-express EGFP and the regulatory T cell-specific transcription factor Foxp3 under the control of the endogenous promoter. EGFP expression accurately identifies the Foxp3+ T cell, and Foxp3 mRNA expression strictly segregates with EGFP+ T cells. These mutant mice may be useful in immunological studies, including studies of regulatory T cell proliferation, localization, and antigen independence during the primary immune response. | ||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
Strain Information
Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Homozygote x Hemizygote (Female x Male) 04-FEB-08 Species laboratory mouse Generation N8+N1F13 (03-DEC-12)
Generation DefinitionsDonating Investigator Talal Chatila, Boston Children's Hospital Description
Homozygous mice are viable and fertile with normal T and B cell development. These "Foxp3EGFP" mice co-express EGFP and the regulatory T cell-specific transcription factor Foxp3 under the control of the endogenous promoter. EGFP expression accurately identifies the Foxp3+ T cell population (more than 97% of Foxp3+ T cells were EGFP+), and Foxp3 mRNA expression strictly segregates with EGFP+ T cells. Due to X-inactivation in females, the number of EGFP+ CD4+ T cells found in the peripheral blood of heterozygous females was approximately half that of hemizygote males. CD4+ EGFP+ cells also exhibit normal regulatory T cell suppression of effector cell proliferation (following stimulation with anti-CD3 and anti-CD28 monoclonal antibodies). Some EGFP expression is noted in a small population of CD8+ thymocytes. These mutant mice may be useful in immunological studies, including studies of regulatory T cell proliferation, localization, and antigen independence during the primary immune response.This allele is also on a C57BL/6 genetic background (see Stock No. 006772).
Development
A targeting vector was designed to place an IRES-EGFP-SV40 polyA sequence immediately downstream of the endogenous Foxp3 translational stop codon but upstream of the endogenous polyA signal. The construct also introduced a loxP-flanked PGKneo cassette into intron 9. This vector was electroporated into 129X1Sv/J-derived SCC10 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and chimeric males were mated with BALB/c females. The PGKneo cassette was removed by mating founder males with Cre-deleter female mice on a BALB/c congenic background. The resulting mice (now harboring this Foxp3EGFP allele) were then backcrossed for 8 generations to BALB/cAnNTac inbred mice prior to arrival at The Jackson Laboratory. During the importation process, this strain may have been bred with BALB/cJ inbred mice (Stock No. 000651) for at least one generation.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000651 BALB/cJ | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (357 strains)
006772 B6.Cg-Foxp3tm2Tch/J
016959 B6.129(Cg)-Foxp3tm4(YFP/cre)Ayr/J 004088 B6.Cg-Foxp3sf/J 018628 B6.Cg-Foxp3tm1Mal/J 019933 B6.Cg-Foxp3tm1Tch/J 014579 B6.NOD-Tg(Foxp3-EGFP/cre)1aJbs/J 016958 B6N.129(Cg)-Foxp3tm3Ayr/J 020654 C.129X1-Foxp3tm3Tch/J 021144 C.Cg-Foxp3tm1Tch/J 008374 C57BL/6-Foxp3tm1Flv/J 006775 NOD.Cg-Foxp3sf/DoiJ 008694 NOD/ShiLt-Tg(Foxp3-EGFP/cre)1cJbs/J 016961 STOCK Foxp3tm9(EGFP/cre/ERT2)Ayr/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested. Diabetes Mellitus, Insulin-Dependent; IDDM (FOXP3)
Immunodysregulation, Polyendocrinopathy, and Enteropathy, X-Linked; (FOXP3)
assigned by genotype
Foxp3tm2Tch/Foxp3+
C.129X1-Foxp3tm2Tch
- immune system phenotype
- *normal* immune system phenotype
Foxp3tm2Tch/Y
C.129X1-Foxp3tm2Tch
- immune system phenotype
- *normal* immune system phenotype
This mouse can be used to support research in many areas including:
GFP relatedImmunology, Inflammation and Autoimmunity Research
Immunodeficiency
T cell deficiency
Research Tools
Fluorescent Proteins
Genetics Research
Tissue/Cell Markers
Tissue/Cell Markers: T cell specific surface marker
Immunology and Inflammation Research
T cell specific surface marker
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Foxp3tm2Tch | ||
|---|---|---|---|
| Allele Name | targeted mutation 2, Talal A Chatila | ||
| Allele Type | Targeted (Reporter) | ||
| Common Name(s) | FoxP3.GFP; Foxp3-GFP KI; Foxp3EGFP; Foxp3IRES-GFP; Foxp3tm2(EGFP)Tch; | ||
| Mutation Made By | Talal Chatila, Boston Children's Hospital | ||
| Strain of Origin | 129X1/SvJ | ||
| ES Cell Line Name | SCC10 | ||
| ES Cell Line Strain | 129X1/SvJ | ||
| Site of Expression | EGFP is expressed in the Foxp3+ T cell population. Some EGFP expression is noted in a small population of CD8+ thymocytes. | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Molecular Note | An IRES-EGFP-SV40 poly A sequence was inserted immediately downstream of the endogenous Foxp3 translational stop codon, but upstream of the endogenous polyA signal, generating a bicistronic locus encoding both Foxp3 and EGFP. Expression of EGFP is restricted to the T cell lineage, primarily to the CD4+ T cell population. [MGI Ref ID J:120713] | ||
| Gene Symbol and Name | Foxp3, forkhead box P3 | ||
| Chromosome | X | ||
| Gene Common Name(s) | AIID; DIETER; IPEX; JM2; PIDX; RGD1562112; XPID; scurfin; scurfy; sf; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Foxp3tm2Tch, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Haribhai D; Lin W; Relland LM; Truong N; Williams CB; Chatila TA. 2007. Regulatory T cells dynamically control the primary immune response to foreign antigen. J Immunol 178(5):2961-72. [PubMed: 17312141] [MGI Ref ID J:120713]
Lin W; Haribhai D; Relland LM; Truong N; Carlson MR; Williams CB; Chatila TA. 2007. Regulatory T cell development in the absence of functional Foxp3. Nat Immunol 8(4):359-68. [PubMed: 17273171] [MGI Ref ID J:120636]
Additional References
Foxp3tm2Tch relatedAbel S; Luckheide N; Westendorf AM; Geffers R; Roers A; Muller W; Sparwasser T; Matuschewski K; Buer J; Hansen W. 2012. Strong impact of CD4+ Foxp3+ regulatory T cells and limited effect of T cell-derived IL-10 on pathogen clearance during Plasmodium yoelii infection. J Immunol 188(11):5467-77. [PubMed: 22544931] [MGI Ref ID J:188734]
Atarashi K; Tanoue T; Shima T; Imaoka A; Kuwahara T; Momose Y; Cheng G; Yamasaki S; Saito T; Ohba Y; Taniguchi T; Takeda K; Hori S; Ivanov II; Umesaki Y; Itoh K; Honda K. 2011. Induction of colonic regulatory T cells by indigenous Clostridium species. Science 331(6015):337-41. [PubMed: 21205640] [MGI Ref ID J:172265]
Benson A; Murray S; Divakar P; Burnaevskiy N; Pifer R; Forman J; Yarovinsky F. 2012. Microbial infection-induced expansion of effector T cells overcomes the suppressive effects of regulatory T cells via an IL-2 deprivation mechanism. J Immunol 188(2):800-10. [PubMed: 22147768] [MGI Ref ID J:180888]
Betts RJ; Prabhu N; Ho AW; Lew FC; Hutchinson PE; Rotzschke O; Macary PA; Kemeny DM. 2012. Influenza a virus infection results in a robust, antigen-responsive, and widely disseminated foxp3+ regulatory T cell response. J Virol 86(5):2817-25. [PubMed: 22205730] [MGI Ref ID J:181233]
Cai SF; Cao X; Hassan A; Fehniger TA; Ley TJ. 2010. Granzyme B is not required for regulatory T cell-mediated suppression of graft-versus-host disease. Blood 115(9):1669-77. [PubMed: 19965675] [MGI Ref ID J:157876]
Cavassani KA; Carson WF 4th; Moreira AP; Wen H; Schaller MA; Ishii M; Lindell DM; Dou Y; Lukacs NW; Keshamouni VG; Hogaboam CM; Kunkel SL. 2010. The post sepsis-induced expansion and enhanced function of regulatory T cells create an environment to potentiate tumor growth. Blood 115(22):4403-11. [PubMed: 20130237] [MGI Ref ID J:161572]
Centuori SM; Trad M; LaCasse CJ; Alizadeh D; Larmonier CB; Hanke NT; Kartchner J; Janikashvili N; Bonnotte B; Larmonier N; Katsanis E. 2012. Myeloid-derived suppressor cells from tumor-bearing mice impair TGF-beta-induced differentiation of CD4+CD25+FoxP3+ Tregs from CD4+CD25-FoxP3- T cells. J Leukoc Biol 92(5):987-97. [PubMed: 22891289] [MGI Ref ID J:189810]
Chen C; Liu Y; Liu Y; Zheng P. 2010. Mammalian target of rapamycin activation underlies HSC defects in autoimmune disease and inflammation in mice. J Clin Invest 120(11):4091-101. [PubMed: 20972332] [MGI Ref ID J:167563]
Chen X; Das R; Komorowski R; Beres A; Hessner MJ; Mihara M; Drobyski WR. 2009. Blockade of interleukin-6 signaling augments regulatory T-cell reconstitution and attenuates the severity of graft-versus-host disease. Blood 114(4):891-900. [PubMed: 19491393] [MGI Ref ID J:150726]
Cho WY; Choi HM; Lee SY; Kim MG; Kim HK; Jo SK. 2010. The role of Tregs and CD11c(+) macrophages/dendritic cells in ischemic preconditioning of the kidney. Kidney Int 78(10):981-92. [PubMed: 20686446] [MGI Ref ID J:184252]
Choi J; Ritchey J; Prior JL; Holt M; Shannon WD; Deych E; Piwnica-Worms DR; DiPersio JF. 2010. In vivo administration of hypomethylating agents mitigate graft-versus-host disease without sacrificing graft-versus-leukemia. Blood 116(1):129-39. [PubMed: 20424188] [MGI Ref ID J:162801]
Chougnet CA; Tripathi P; Lages CS; Raynor J; Sholl A; Fink P; Plas DR; Hildeman DA. 2011. A major role for Bim in regulatory T cell homeostasis. J Immunol 186(1):156-63. [PubMed: 21098226] [MGI Ref ID J:168007]
Chung HS; Lee JH; Kim H; Lee HJ; Kim SH; Kwon HK; Im SH; Bae H. 2010. Foxp3 is a novel repressor of microglia activation. Glia 58(10):1247-56. [PubMed: 20544860] [MGI Ref ID J:168043]
Clambey ET; McNamee EN; Westrich JA; Glover LE; Campbell EL; Jedlicka P; de Zoeten EF; Cambier JC; Stenmark KR; Colgan SP; Eltzschig HK. 2012. Hypoxia-inducible factor-1 alpha-dependent induction of FoxP3 drives regulatory T-cell abundance and function during inflammatory hypoxia of the mucosa. Proc Natl Acad Sci U S A 109(41):E2784-93. [PubMed: 22988108] [MGI Ref ID J:190110]
Cong Y; Feng T; Fujihashi K; Schoeb TR; Elson CO. 2009. A dominant, coordinated T regulatory cell-IgA response to the intestinal microbiota. Proc Natl Acad Sci U S A 106(46):19256-61. [PubMed: 19889972] [MGI Ref ID J:154759]
Deiuliis JA; Kampfrath T; Zhong J; Oghumu S; Maiseyeu A; Chen LC; Sun Q; Satoskar AR; Rajagopalan S. 2012. Pulmonary T cell activation in response to chronic particulate air pollution. Am J Physiol Lung Cell Mol Physiol 302(4):L399-409. [PubMed: 22160305] [MGI Ref ID J:183449]
Ehrentraut H; Westrich JA; Eltzschig HK; Clambey ET. 2012. Adora2b adenosine receptor engagement enhances regulatory T cell abundance during endotoxin-induced pulmonary inflammation. PLoS One 7(2):e32416. [PubMed: 22389701] [MGI Ref ID J:185292]
Feng T; Cong Y; Qin H; Benveniste EN; Elson CO. 2010. Generation of mucosal dendritic cells from bone marrow reveals a critical role of retinoic Acid. J Immunol 185(10):5915-25. [PubMed: 20944006] [MGI Ref ID J:165627]
Fyhrquist N; Lehtimaki S; Lahl K; Savinko T; Lappetelainen AM; Sparwasser T; Wolff H; Lauerma A; Alenius H. 2012. Foxp3+ cells control Th2 responses in a murine model of atopic dermatitis. J Invest Dermatol 132(6):1672-80. [PubMed: 22402436] [MGI Ref ID J:188756]
Guo Z; Khattar M; Schroder PM; Miyahara Y; Wang G; He X; Chen W; Stepkowski SM. 2013. A Dynamic Dual Role of IL-2 Signaling in the Two-Step Differentiation Process of Adaptive Regulatory T Cells. J Immunol 190(7):3153-62. [PubMed: 23427250] [MGI Ref ID J:194525]
Guo Z; Wen Z; Qin A; Zhou Y; Liao Z; Liu Z; Liang Y; Ren T; Xu L. 2013. Antisense Oligonucleotide Treatment Enhances the Recovery of Acute Lung Injury through IL-10-Secreting M2-like Macrophage-Induced Expansion of CD4+ Regulatory T Cells. J Immunol 190(8):4337-48. [PubMed: 23514739] [MGI Ref ID J:195286]
Hams E; McCarron MJ; Amu S; Yagita H; Azuma M; Chen L; Fallon PG. 2011. Blockade of B7-H1 (programmed death ligand 1) enhances humoral immunity by positively regulating the generation of T follicular helper cells. J Immunol 186(10):5648-55. [PubMed: 21490158] [MGI Ref ID J:173223]
Haribhai D; Williams JB; Jia S; Nickerson D; Schmitt EG; Edwards B; Ziegelbauer J; Yassai M; Li SH; Relland LM; Wise PM; Chen A; Zheng YQ; Simpson PM; Gorski J; Salzman NH; Hessner MJ; Chatila TA; Williams CB. 2011. A requisite role for induced regulatory T cells in tolerance based on expanding antigen receptor diversity. Immunity 35(1):109-22. [PubMed: 21723159] [MGI Ref ID J:174377]
Idoyaga J; Fiorese C; Zbytnuik L; Lubkin A; Miller J; Malissen B; Mucida D; Merad M; Steinman RM. 2013. Specialized role of migratory dendritic cells in peripheral tolerance induction. J Clin Invest :. [PubMed: 23298832] [MGI Ref ID J:194499]
Jeon SG; Kayama H; Ueda Y; Takahashi T; Asahara T; Tsuji H; Tsuji NM; Kiyono H; Ma JS; Kusu T; Okumura R; Hara H; Yoshida H; Yamamoto M; Nomoto K; Takeda K. 2012. Probiotic Bifidobacterium breve induces IL-10-producing Tr1 cells in the colon. PLoS Pathog 8(5):e1002714. [PubMed: 22693446] [MGI Ref ID J:195381]
Kaminitz A; Yolcu ES; Askenasy EM; Stein J; Yaniv I; Shirwan H; Askenasy N. 2011. Effector and Naturally Occurring Regulatory T Cells Display No Abnormalities in Activation Induced Cell Death in NOD Mice. PLoS One 6(6):e21630. [PubMed: 21738739] [MGI Ref ID J:174547]
Lathrop SK; Bloom SM; Rao SM; Nutsch K; Lio CW; Santacruz N; Peterson DA; Stappenbeck TS; Hsieh CS. 2011. Peripheral education of the immune system by colonic commensal microbiota. Nature 478(7368):250-4. [PubMed: 21937990] [MGI Ref ID J:177128]
Lee H; Nho D; Chung HS; Lee H; Shin MK; Kim SH; Bae H. 2010. CD4+CD25+ regulatory T cells attenuate cisplatin-induced nephrotoxicity in mice. Kidney Int 78(11):1100-9. [PubMed: 20463654] [MGI Ref ID J:184263]
Liu Z; Falo LD Jr; You Z. 2011. Knockdown of HMGB1 in tumor cells attenuates their ability to induce regulatory T cells and uncovers naturally acquired CD8 T cell-dependent antitumor immunity. J Immunol 187(1):118-25. [PubMed: 21642542] [MGI Ref ID J:176186]
Lobo PI; Bajwa A; Schlegel KH; Vengal J; Lee SJ; Huang L; Ye H; Deshmukh U; Wang T; Pei H; Okusa MD. 2012. Natural IgM anti-leukocyte autoantibodies attenuate excess inflammation mediated by innate and adaptive immune mechanisms involving Th-17. J Immunol 188(4):1675-85. [PubMed: 22262657] [MGI Ref ID J:181303]
Mashayekhi M; Sandau MM; Dunay IR; Frickel EM; Khan A; Goldszmid RS; Sher A; Ploegh HL; Murphy TL; Sibley LD; Murphy KM. 2011. CD8alpha(+) dendritic cells are the critical source of interleukin-12 that controls acute infection by Toxoplasma gondii tachyzoites. Immunity 35(2):249-59. [PubMed: 21867928] [MGI Ref ID J:176232]
Mayne CG; Spanier JA; Relland LM; Williams CB; Hayes CE. 2011. 1,25-Dihydroxyvitamin D3 acts directly on the T lymphocyte vitamin D receptor to inhibit experimental autoimmune encephalomyelitis. Eur J Immunol 41(3):822-32. [PubMed: 21287548] [MGI Ref ID J:175424]
Morlacchi S; Soldani C; Viola A; Sarukhan A. 2011. Self-antigen presentation by mouse B cells results in regulatory T-cell induction rather than anergy or clonal deletion. Blood 118(4):984-91. [PubMed: 21652680] [MGI Ref ID J:174859]
Niess JH; Adler G. 2010. Enteric flora expands gut lamina propria CX3CR1+ dendritic cells supporting inflammatory immune responses under normal and inflammatory conditions. J Immunol 184(4):2026-37. [PubMed: 20089703] [MGI Ref ID J:159471]
Oh S; Aitken M; Simons DM; Basehoar A; Garcia V; Kropf E; Caton AJ. 2012. Requirement for diverse TCR specificities determines regulatory T cell activity in a mouse model of autoimmune arthritis. J Immunol 188(9):4171-80. [PubMed: 22450809] [MGI Ref ID J:188463]
Pallotta MT; Orabona C; Volpi C; Vacca C; Belladonna ML; Bianchi R; Servillo G; Brunacci C; Calvitti M; Bicciato S; Mazza EM; Boon L; Grassi F; Fioretti MC; Fallarino F; Puccetti P; Grohmann U. 2011. Indoleamine 2,3-dioxygenase is a signaling protein in long-term tolerance by dendritic cells. Nat Immunol 12(9):870-8. [PubMed: 21804557] [MGI Ref ID J:176469]
Patterson SJ; Han JM; Garcia R; Assi K; Gao T; O'Neill A; Newton AC; Levings MK. 2011. Cutting edge: PHLPP regulates the development, function, and molecular signaling pathways of regulatory T cells. J Immunol 186(10):5533-7. [PubMed: 21498666] [MGI Ref ID J:173220]
Procaccini C; De Rosa V; Galgani M; Abanni L; Cali G; Porcellini A; Carbone F; Fontana S; Horvath TL; La Cava A; Matarese G. 2010. An oscillatory switch in mTOR kinase activity sets regulatory T cell responsiveness. Immunity 33(6):929-41. [PubMed: 21145759] [MGI Ref ID J:167293]
Procaccini C; De Rosa V; Galgani M; Carbone F; Cassano S; Greco D; Qian K; Auvinen P; Cali G; Stallone G; Formisano L; La Cava A; Matarese G. 2012. Leptin-induced mTOR activation defines a specific molecular and transcriptional signature controlling CD4+ effector T cell responses. J Immunol 189(6):2941-53. [PubMed: 22904304] [MGI Ref ID J:189915]
Ramakrishna C; Newo AN; Shen YW; Cantin E. 2011. Passively administered pooled human immunoglobulins exert IL-10 dependent anti-inflammatory effects that protect against fatal HSV encephalitis. PLoS Pathog 7(6):e1002071. [PubMed: 21655109] [MGI Ref ID J:182197]
Ray A; Basu S; Williams CB; Salzman NH; Dittel BN. 2012. A novel IL-10-independent regulatory role for B cells in suppressing autoimmunity by maintenance of regulatory T cells via GITR ligand. J Immunol 188(7):3188-98. [PubMed: 22368274] [MGI Ref ID J:183107]
Relland LM; Williams JB; Relland GN; Haribhai D; Ziegelbauer J; Yassai M; Gorski J; Williams CB. 2012. The TCR repertoires of regulatory and conventional T cells specific for the same foreign antigen are distinct. J Immunol 189(7):3566-74. [PubMed: 22933635] [MGI Ref ID J:190344]
Rivas EI; Driver JP; Garabatos N; Presa M; Mora C; Rodriguez F; Serreze DV; Stratmann T. 2011. Targeting of a T cell agonist Peptide to lysosomes by DNA vaccination induces tolerance in the nonobese diabetic mouse. J Immunol 186(7):4078-87. [PubMed: 21346228] [MGI Ref ID J:170837]
Sahu RP; Petrache I; Van Demark MJ; Rashid BM; Ocana JA; Tang Y; Yi Q; Turner MJ; Konger RL; Travers JB. 2013. Cigarette Smoke Exposure Inhibits Contact Hypersensitivity via the Generation of Platelet-Activating Factor Agonists. J Immunol 190(5):2447-54. [PubMed: 23355733] [MGI Ref ID J:193474]
Salti SM; Hammelev EM; Grewal JL; Reddy ST; Zemple SJ; Grossman WJ; Grayson MH; Verbsky JW. 2011. Granzyme B regulates antiviral CD8+ T cell responses. J Immunol 187(12):6301-9. [PubMed: 22084442] [MGI Ref ID J:180386]
Sawant DV; Sehra S; Nguyen ET; Jadhav R; Englert K; Shinnakasu R; Hangoc G; Broxmeyer HE; Nakayama T; Perumal NB; Kaplan MH; Dent AL. 2012. Bcl6 controls the Th2 inflammatory activity of regulatory T cells by repressing Gata3 function. J Immunol 189(10):4759-69. [PubMed: 23053511] [MGI Ref ID J:190587]
Schallenberg S; Petzold C; Tsai PY; Sparwasser T; Kretschmer K. 2012. Vagaries of fluorochrome reporter gene expression in Foxp3+ regulatory T cells. PLoS One 7(8):e41971. [PubMed: 22879902] [MGI Ref ID J:189833]
Schallenberg S; Tsai PY; Riewaldt J; Kretschmer K. 2010. Identification of an immediate Foxp3(-) precursor to Foxp3(+) regulatory T cells in peripheral lymphoid organs of nonmanipulated mice. J Exp Med 207(7):1393-407. [PubMed: 20584884] [MGI Ref ID J:163388]
Schmitt EG; Haribhai D; Williams JB; Aggarwal P; Jia S; Charbonnier LM; Yan K; Lorier R; Turner A; Ziegelbauer J; Georgiev P; Simpson P; Salzman NH; Hessner MJ; Broeckel U; Chatila TA; Williams CB. 2012. IL-10 Produced by Induced Regulatory T Cells (iTregs) Controls Colitis and Pathogenic Ex-iTregs during Immunotherapy. J Immunol 189(12):5638-48. [PubMed: 23125413] [MGI Ref ID J:190976]
Surls J; Nazarov-Stoica C; Kehl M; Olsen C; Casares S; Brumeanu TD. 2012. Increased membrane cholesterol in lymphocytes diverts T-cells toward an inflammatory response. PLoS One 7(6):e38733. [PubMed: 22723880] [MGI Ref ID J:187823]
Tessmer MS; Reilly EC; Brossay L. 2011. Salivary gland NK cells are phenotypically and functionally unique. PLoS Pathog 7(1):e1001254. [PubMed: 21249177] [MGI Ref ID J:172475]
Venet F; Chung CS; Huang X; Lomas-Neira J; Chen Y; Ayala A. 2009. Lymphocytes in the development of lung inflammation: a role for regulatory CD4+ T cells in indirect pulmonary lung injury. J Immunol 183(5):3472-80. [PubMed: 19641139] [MGI Ref ID J:151875]
Xiong Y; Khanna S; Grzenda AL; Sarmento OF; Svingen PA; Lomberk GA; Urrutia RA; Faubion WA Jr. 2012. Polycomb antagonizes p300/CREB-binding protein-associated factor to silence FOXP3 in a Kruppel-like factor-dependent manner. J Biol Chem 287(41):34372-85. [PubMed: 22896699] [MGI Ref ID J:191582]
Yokosuka T; Kobayashi W; Takamatsu M; Sakata-Sogawa K; Zeng H; Hashimoto-Tane A; Yagita H; Tokunaga M; Saito T. 2010. Spatiotemporal basis of CTLA-4 costimulatory molecule-mediated negative regulation of T cell activation. Immunity 33(3):326-39. [PubMed: 20870175] [MGI Ref ID J:164647]
Zaiss DM; van Loosdregt J; Gorlani A; Bekker CP; Grone A; Sibilia M; van Bergen En Henegouwen PM; Roovers RC; Coffer PJ; Sijts AJ. 2013. Amphiregulin Enhances Regulatory T Cell-Suppressive Function via the Epidermal Growth Factor Receptor. Immunity 38(2):275-84. [PubMed: 23333074] [MGI Ref ID J:193394]
Zhang W; Zhang D; Shen M; Liu Y; Tian Y; Thomson AW; Lee WP; Zheng XX. 2010. Combined administration of a mutant TGF-beta1/Fc and rapamycin promotes induction of regulatory T cells and islet allograft tolerance. J Immunol 185(8):4750-9. [PubMed: 20844194] [MGI Ref ID J:164884]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry This mutant allele is located on the X chromosome. When maintaining a live colony, homozygous females are bred with hemizygous males. Mating System Homozygote x Hemizygote (Female x Male) 04-FEB-08 Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $177.00 Male Hemizygous for Foxp3tm2Tch $177.00 Female Homozygous for Foxp3tm2Tch
Price per Pair (US dollars $) Pair Genotype $354.00 Homozygous for Foxp3tm2Tch x Hemizygous for Foxp3tm2Tch Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
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Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $230.10 Male Hemizygous for Foxp3tm2Tch $230.10 Female Homozygous for Foxp3tm2Tch
Price per Pair (US dollars $) Pair Genotype $460.20 Homozygous for Foxp3tm2Tch x Hemizygous for Foxp3tm2Tch Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000651 BALB/cJ | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
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| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.
No Liability
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.