Strain Name: |
C.Cg-Foxp3tm2Tch/J |
|---|---|
Stock Number: |
006769 |
Availability: | Repository- Live |
General Terms and Conditions |
| Strain Common Names | C.Cg-Foxp3tm2(EGFP)Tch/J; |
| Genes & Alleles | Foxp3; Foxp3tm2Tch; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Mating System Homozygote x Hemizygote (Female x Male) Species laboratory mouse Donating Investigator Talal Chatila, David Geffen School of Medicine, UCLA Generation N8+N1+F2 (03-DEC-07) Strain Description
Homozygous mice are viable and fertile with normal T and B cell development. These "Foxp3EGFP" mice co-express EGFP and the regulatory T cell-specific transcription factor Foxp3 under the control of the endogenous promoter. EGFP expression accurately identifies the Foxp3+ T cell population (more than 97% of Foxp3+ T cells were EGFP+), and Foxp3 mRNA expression strictly segregates with EGFP+ T cells. Due to X-inactivation in females, the number of EGFP+ CD4+ T cells found in the peripheral blood of heterozygous females was approximately half that of hemizygote males. CD4+ EGFP+ cells also exhibit normal regulatory T cell suppression of effector cell proliferation (following stimulation with anti-CD3 and anti-CD28 monoclonal antibodies). Some EGFP expression is noted in a small population of CD8+ thymocytes. These mutant mice may be useful in immunological studies, including studies of regulatory T cell proliferation, localization, and antigen independence during the primary immune response.This allele is also on a C57BL/6 genetic background (see Stock No. 006772).
Strain Development
A targeting vector was designed to place an IRES-EGFP-SV40 polyA sequence immediately downstream of the endogenous Foxp3 translational stop codon but upstream of the endogenous polyA signal. The construct also introduced a loxP-flanked PGKneo cassette into intron 9. This vector was electroporated into 129X1Sv/J-derived SCC10 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and chimeric males were mated with BALB/c females. The PGKneo cassette was removed by mating founder males with Cre-deleter female mice on a BALB/c congenic background. The resulting mice (now harboring this Foxp3EGFP allele) were then backcrossed for 8 generations to BALB/cAnNTac inbred mice prior to arrival at The Jackson Laboratory. During the importation process, this strain may have been bred with BALB/cJ inbred mice (Stock No. 000651) for at least one generation.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Foxp3tm2Tch | ||
|---|---|---|---|
| Allele Name | targeted mutation 2, Talal A Chatila | ||
| Common Name(s) | Foxp3EGFP; Foxp3tm2(EGFP)Tch; | ||
| Mutation Made By | Talal Chatila, David Geffen School of Medicine, UCLA | ||
| Strain of Origin | 129X1/SvJ | ||
| ES Cell Line Name | Other (see notes) | ||
| ES Cell Line Strain | 129 | ||
| Gene Symbol and Name | Foxp3, forkhead box P3 | ||
| Chromosome | X | ||
| Gene Common Name(s) | AIID; DIETER; IPEX; JM2; MGC141961; MGC141963; PIDX; RGD1562112; XPID; scurfin; scurfy; sf; | ||
| General Note | ES cell line = SSC10 | ||
| Molecular Note | An IRES-EGFP-SV40 poly A sequence was inserted immediately downstream of the endogenous Foxp3 translational stop codon, but upstream of the endogenous polyA signal, generating a bicistronic locus encoding both Foxp3 and EGFP. Expression of EGFP is restricted to the T cell lineage, primarily to the CD4+ T cell population. [J:120713] | ||
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000651 BALB/cJ | (approximate) | |
| Considerations for Choosing Controls | ||
Colony Maintenance
| Breeding & Husbandry | This mutant allele is located on the X chromosome. When maintaining a live colony, homozygous females are bred with hemizygous males. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (135 strains)
006772 B6.Cg-Foxp3tm2Tch/J
004088 B6.Cg-Foxp3sf/J 006775 NOD.Cg-Foxp3sf/DoiJ
Additional Web Information
Congenic Nomenclature
Fluorescent Proteins/lacZ Systems
Animal Health Reports
Room Number AX11
Research Applications
This mouse can be used to support research in many areas including:
Immunology and Inflammation Research
Immunodeficiency (T cell deficiency)
Research Tools
Fluorescent Proteins
Genetics Research (Tissue/Cell Markers: T cell specific surface marker)
Immunology and Inflammation Research (T cell specific surface marker)
References
Selected Reference(s)
Additional ReferencesHaribhai D; Lin W; Relland LM; Truong N; Williams CB; Chatila TA. 2007. Regulatory T cells dynamically control the primary immune response to foreign antigen. J Immunol 178(5):2961-72. [PubMed: 17312141] [J:120713]
Lin W; Haribhai D; Relland LM; Truong N; Carlson MR; Williams CB; Chatila TA. 2007. Regulatory T cell development in the absence of functional Foxp3. Nat Immunol 8(4):359-68. [PubMed: 17273171] [J:120636]
Price and Supply Information
| Strain Name: | C.Cg-Foxp3tm2Tch/J |
| Stock Number: | 006769 |
Price Details
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Supply Details
| Standard Supply | Repository-Live. No age specifications accepted. Colony sized to produce minimal quantities (typically up to 6 mice) upon order receipt; one order per strain. Expected delivery: 1-3 months. Larger quantities or custom orders arranged upon request. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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